Biosynthesis and production of quercitols and their application in the production of pharmaceuticals: current status and prospects.

abstract：:Biodegradation of petroleum compounds in saline environments seems intricate and needs more attention. In this study, tetracosane was used to enrich alkane-degrading bacteria from oil-contaminated saline soils. Among the isolates, strain Qtet3, with the highest 16s rRNA gene sequence similarity to Alcanivorax dieselol...

journal_title：Applied microbiology and biotechnology

authors： Dastgheib SM,Amoozegar MA,Khajeh K,Ventosa A

更新日期：2011-04-01 00:00:00

abstract：:Leavening ability in sweet dough is required for the commercial applications of baker's yeast. This property depends on many factors, such as glycolytic activity, sucrase activity, and osmotolerance. This study explored the importance of sucrase level on the leavening ability of baker's yeast in sweet dough. Furthermo...

journal_title：Applied microbiology and biotechnology

authors： Zhang CY,Lin X,Feng B,Liu XE,Bai XW,Xu J,Pi L,Xiao DG

更新日期：2016-07-01 00:00:00

abstract：:In the Candida antarctica lipase B-catalyzed hydrolysis of (R,S)-azolides derived from (R,S)-N-protected proline in water-saturated methyl tert-butyl ether (MTBE), high enzyme activity with excellent enantioselectivity (V (S) V (R) ...

journal_title：Applied microbiology and biotechnology

authors： Wu CH,Pen CW,Wang PY,Tsai SW

更新日期：2013-02-01 00:00:00

abstract：:To understand the effect of daily bioaugmentation in full-scale grease interceptors (GIs), we compared the microbial communities occurring in two full-scale GIs during bioaugmented and non-bioaugmented cycles. The changes in microbial communities were determined using terminal restriction fragment length polymorphism ...

journal_title：Applied microbiology and biotechnology

authors： He X,So MJ,de Los Reyes FL 3rd

更新日期：2016-08-01 00:00:00

abstract：:Arthrobacter sp. strain KI72 grows on a 6-aminohexanoate oligomer, which is a by-product of nylon-6 manufacturing, as a sole source of carbon and nitrogen. We cloned the two genes, nylD 1 and nylE 1 , responsible for 6-aminohexanoate metabolism on the basis of the draft genomic DNA sequence of strain KI72. We amplifie...

journal_title：Applied microbiology and biotechnology

authors： Takehara I,Fujii T,Tanimoto Y,Kato DI,Takeo M,Negoro S

更新日期：2018-01-01 00:00:00

abstract：:The 1,2-propanediol (1,2-PD) inducible membrane-bound L-pantoyl lactone (L-PL) dehydrogenase (LPLDH) has been isolated from Rhodococcus erythropolis AKU2103 (Kataoka et al. in Eur J Biochem 204:799, 1992). Based on the N-terminal amino acid sequence of LPLDH and the highly conserved amino acid sequence in homology sea...

journal_title：Applied microbiology and biotechnology

authors： Si D,Urano N,Nozaki S,Honda K,Shimizu S,Kataoka M

更新日期：2012-07-01 00:00:00

abstract：:Two bacterial strains, Ralstonia sp. LD35 and Pseudomonas putida DSM 1868, were assayed for their ability to degrade the monocyclic aromatic compounds commonly found in olive mill wastewaters (OMWs). The goal was to study the possibility of employing the two strains in the removal of these recalcitrant and toxic compo...

journal_title：Applied microbiology and biotechnology

authors： Di Gioia D,Fava F,Bertin L,Marchetti L

更新日期：2001-05-01 00:00:00

abstract：:Functional screening for lipolytic enzymes at low temperatures resulted in the isolation of the novel cold-active esterases, EstM-N1 and EstM-N2, from a metagenomic DNA library of arctic soil samples. EstM-N1 and EstM-N2 were 395 and 407 amino acids in length, respectively, and showed the highest similarity to class C...

journal_title：Applied microbiology and biotechnology

authors： Yu EY,Kwon MA,Lee M,Oh JY,Choi JE,Lee JY,Song BK,Hahm DH,Song JK

更新日期：2011-04-01 00:00:00

abstract：:The gene for the thermostable pyruvate kinase of Microbispora thermodiastatica IFO 14046, a moderate thermophilic actinomycete, was cloned in Escherichia coli. This gene consists of an open reading frame of 1422 nucleotides and encodes a protein of 474 amino acids with molecular mass of 50,805 Da. The open reading fra...

journal_title：Applied microbiology and biotechnology

authors： Arai A,Masuda S,Matsuyama A,Murakami S,Nakajima M

更新日期：1998-03-01 00:00:00

abstract：:When sucrose was used as the carbon source, the Basidiomycete Coprinopsis cinerea showed poor growth and low laccase activity in pure culture, but greatly enhanced the level of laccase activity (>1800 U/L) during coculture with the Mucoromycete Gongronella sp. w5. As a result, the mechanism of laccase overproduction i...

journal_title：Applied microbiology and biotechnology

authors： Hu J,Zhang Y,Xu Y,Sun Q,Liu J,Fang W,Xiao Y,Kües U,Fang Z

更新日期：2019-01-01 00:00:00

abstract：:Guanosine 5'-triphosphate (GTP) is the key substrate for biosynthesis of guanosine 5'-diphosphate (GDP)-L-fucose. In this study, improvement of GDP-L-fucose production was attempted by manipulating the biosynthetic pathway for guanosine nucleotides in recombinant Escherichia coli-producing GDP-L-fucose. The effects of...

journal_title：Applied microbiology and biotechnology

authors： Lee WH,Shin SY,Kim MD,Han NS,Seo JH

更新日期：2012-03-01 00:00:00

abstract：:Both aerobic methane-oxidizing bacteria (MOB) and nitrite-dependent anaerobic methane oxidation (n-damo) bacteria can play an important role in mitigating the methane emission produced in anoxic sediment layers to the atmosphere. However, the environmental factors regulating the distribution of these methane-oxidizing...

journal_title：Applied microbiology and biotechnology

authors： Liu Y,Zhang J,Zhao L,Li Y,Yang Y,Xie S

更新日期：2015-03-01 00:00:00

abstract：:Diosgenin transformation was studied in Streptomyces virginiae IBL-14, a soil-dwelling bacterium with diosgenin-degrading capacity. All of the derivatives isolated were identified as 4-ene-3-keto steroids. We cloned ChoL, a fragment of a cholesterol oxidase from S. virginiae IBL-14, and used gene-disruption techniques...

journal_title：Applied microbiology and biotechnology

authors： Li B,Wang W,Wang FQ,Wei DZ

更新日期：2010-02-01 00:00:00

abstract：:The fungus Colletotrichum lindemuthianum is the causal agent of anthracnose in the common bean (Phaseolus vulgaris), and anthracnose is one of the most devastating diseases of this plant species. However, little is known about the proteins that are essential for the fungus-plant interactions. Knowledge of the fungus' ...

journal_title：Applied microbiology and biotechnology

authors： de Queiroz CB,Correia HLN,Santana MF,Batista DS,Vidigal PMP,Brommonschenkel SH,de Queiroz MV

更新日期：2019-03-01 00:00:00

abstract：:A widespread and hitherto by far underexploited potential among ecologically diverse fungi to pretreat wheat straw and digestate from maize silage in the future perspective of using such lignocellulosic feedstock for fermentative bioenergy production was inferred from a screening of nine freshwater ascomycetes, 76 iso...

journal_title：Applied microbiology and biotechnology

authors： Singh S,Harms H,Schlosser D

更新日期：2014-04-01 00:00:00

abstract：:Soluble expression of recombinant therapeutic proteins in Escherichia coli (E. coli) has been a challenging task in biopharmaceutical development. In this study, a novel self-cleavable tag Zbasic-intein has been constructed for the soluble expression and purification of a recombinant cytokine, human interleukin-15 (IL...

journal_title：Applied microbiology and biotechnology

authors： Shi S,Chen H,Jiang H,Xie Y,Zhang L,Li N,Zhu C,Chen J,Luo H,Wang J,Feng L,Lu H,Zhu J

更新日期：2017-02-01 00:00:00

abstract：:N-acetyl glutamate kinase (NAGK) is a key enzyme in the synthesis of L-arginine that is inhibited by its end product L-arginine in Corynebacterium glutamicum (C. glutamicum). In this study, the potential binding sites of arginine and the residues essential for its inhibition were identified by homology modeling, inhib...

journal_title：Applied microbiology and biotechnology

authors： Huang Y,Zhang H,Tian H,Li C,Han S,Lin Y,Zheng S

更新日期：2015-09-01 00:00:00

abstract：:The merC gene from the Tn21-encoded mer operon has potential uses as a molecular tool for bioremediation. It was overexpressed as the fusion proteins MerC-Sso1p or MerC-Vam3p in Saccharomyces cerevisiae. Green fluorescent protein (GFP)-MerC-Sso1p fusion proteins located primarily in the plasma membrane, although some ...

journal_title：Applied microbiology and biotechnology

authors： Kiyono M,Miyahara K,Sone Y,Pan-Hou H,Uraguchi S,Nakamura R,Sakabe K

更新日期：2010-03-01 00:00:00

abstract：:The present work created an esterase variant from Rhodobacter sphaeroides (RspE) with enhanced selectivity in hydrolytic kinetic resolutions by directed evolution. A "model" substrate, methyl mandelate, was introduced in the high-throughput screening procedure. E values of a variant CH (Asn62Cys/Leu145His) for six dif...

journal_title：Applied microbiology and biotechnology

authors： Ma J,Wu L,Guo F,Gu J,Tang X,Jiang L,Liu J,Zhou J,Yu H

更新日期：2013-06-01 00:00:00

abstract：:Paddy fields are a significant source of methane and contribute up to 20% of total methane emissions from wetland ecosystems. These inundated, anoxic soils featuring abundant nitrogen compounds and methane are an ideal niche for nitrate-dependent anaerobic methanotrophs. After 2 years of enrichment with a continuous s...

journal_title：Applied microbiology and biotechnology

authors： Vaksmaa A,Guerrero-Cruz S,van Alen TA,Cremers G,Ettwig KF,Lüke C,Jetten MSM

更新日期：2017-09-01 00:00:00

abstract：:We report the study of the dynamics of substrate utilization by the genetic modified strain Yarrowia lipolytica H222-S4(p67ICL1) T5. In contrast to its wild-type equivalent, this recombinant strain is able to excrete the sucrose cleaving enzyme invertase. Both the sucrose degradation rate and the glucose and fructose ...

journal_title：Applied microbiology and biotechnology

authors： Moeller L,Zehnsdorf A,Aurich A,Bley T,Strehlitz B

更新日期：2012-02-01 00:00:00

abstract：:AmyC, a glycoside hydrolase family 57 (GH57) enzyme of Thermotoga maritima MSB8, has previously been identified as an intracellular α-amylase playing a role in either maltodextrin utilization or storage polysaccharide metabolism. However, the α-amylase specificity of AmyC is questionable as extensive phylogenetic anal...

journal_title：Applied microbiology and biotechnology

authors： Zhang X,Leemhuis H,Janeček Š,Martinovičová M,Pijning T,van der Maarel MJEC

更新日期：2019-08-01 00:00:00

abstract：:Laccases are blue multicopper oxidases with potential applications in environmental and industrial biotechnology. In this study, a new bacterial laccase gene of 1.32 kb was obtained from a marine microbial metagenome of the South China Sea by using a sequence screening strategy. The protein (named as Lac15) of 439 ami...

journal_title：Applied microbiology and biotechnology

authors： Fang Z,Li T,Wang Q,Zhang X,Peng H,Fang W,Hong Y,Ge H,Xiao Y

更新日期：2011-02-01 00:00:00

abstract：:A new medium supplement mixture, PPRF92, has been developed to enable the serial subculture of human diploid fibroblasts (MRC-5 cells) on microcarriers. Furthermore, the PPRF92 supplements enable cell growth at serum levels as low as 1%. Through an optimization programme, the PPRF92 supplements have evolved into a sim...

journal_title：Applied microbiology and biotechnology

authors： Forestell SP,Kalogerakis N,Behie LA

更新日期：1992-11-01 00:00:00

abstract：:Because engineering of the 101.016-bp megaplasmid pKB1 of Gordonia westfalica Kb1 failed due to the absence of an effective transfer system, pKB1 was transferred by conjugation from G. westfalica Kb1 to a kanamycin-resistant mutant of Rhodococcus opacus PD630 at a frequency of about 6.2 x 10(-8) events per recipient c...

journal_title：Applied microbiology and biotechnology

authors： Bröker D,Arenskötter M,Steinbüchel A

更新日期：2008-01-01 00:00:00

abstract：:With advances in the development of DNA vaccines and gene therapy, there is a growing need for plasmid DNA with high quality for fundamental research and clinical trials. In this report, a scalable automated process for large-scale preparation of plasmid is described. This process is based on alkaline lysis and can be...

journal_title：Applied microbiology and biotechnology

authors： Li X,Jin H,Wu Z,Rayner S,Yin J,Yu Y,Zhang W,He Z,Wang C,Wang B

更新日期：2007-07-01 00:00:00

abstract：:Cytochromes P450 (CYPs) belong to the superfamily of heme b containing monooxygenases with currently more than 21,000 members. These enzymes accept a vast range of organic molecules and catalyze diverse reactions. These extraordinary capabilities of CYP systems that are unmet by other enzymes make them attractive for ...

journal_title：Applied microbiology and biotechnology

authors： Bernhardt R,Urlacher VB

更新日期：2014-01-01 00:00:00

abstract：:This study investigated the effects of vanillin on methanogenesis and rumen fermentation, and the responses of ruminal protein-degrading bacteria to vanillin (at concentrations of 0, 0.76 and 1.52 g/L), essential oils (clove oil, 1 g/L; origanum oil, 0.50 g/L, and peppermint oil, 1 g/L), and quillaja saponin (at conce...

journal_title：Applied microbiology and biotechnology

authors： Patra AK,Yu Z

更新日期：2014-01-01 00:00:00

abstract：:Due to efficient photosynthetic capability, robust growth, and clear genetic background, cyanobacteria are recently used for production of different biofuel and biochemical molecules by genetic engineering and showed great potentials as the next-generation microbial cell factory. For improving the production of bio-pr...

journal_title：Applied microbiology and biotechnology

authors： Tan X,Liang F,Cai K,Lu X

更新日期：2013-07-01 00:00:00

abstract：:Mediated electrochemical detection of catabolism in prokaryotic cells is well documented; however, the application of this technique to eukaryotic cells has received less attention. Two catabolic substrate-dependent mediated electrochemical signals were detected in the yeast Saccharomyces cerevisiae. The signal using ...

journal_title：Applied microbiology and biotechnology

authors： Baronian KH,Downard AJ,Lowen RK,Pasco N

更新日期：2002-10-01 00:00:00