Cholesterol oxidase ChoL is a critical enzyme that catalyzes the conversion of diosgenin to 4-ene-3-keto steroids in Streptomyces virginiae IBL-14.

abstract：:Furfural is one of the major inhibitors generated during sugar production from cellulosic materials and, as an aldehyde, inhibits various cellular activities of microorganisms used, leading to prolonged lag time during ethanologenic fermentation. Since Saccharomyces cerevisiae strains tolerant to furfural are of great...

journal_title：Applied microbiology and biotechnology

authors： Kim HS,Kim NR,Kim W,Choi W

更新日期：2012-07-01 00:00:00

abstract：:Overproduction of recombinant secretory proteins triggers numerous physiological perturbations. Depending on a given heterologous protein characteristics, the producer cell is faced with different challenges which lead to varying responses in terms of its physiology and the target protein production rate. In the prese...

journal_title：Applied microbiology and biotechnology

authors： Korpys-Woźniak P,Kubiak P,Białas W,Celińska E

更新日期：2020-11-01 00:00:00

abstract：:The filamentous fungus Aspergillus flavus is an agriculturally important opportunistic plant pathogen that produces potent carcinogenic compounds called aflatoxins. We identified the A. flavus rtfA gene, the ortholog of rtf1 in Saccharomyces cerevisiae and rtfA in Aspergillus nidulans. Interestingly, rtfA has multiple...

journal_title：Applied microbiology and biotechnology

authors： Lohmar JM,Harris-Coward PY,Cary JW,Dhingra S,Calvo AM

更新日期：2016-06-01 00:00:00

abstract：:Escherichia coli BL21, expressing a quintuple mutant of P450(BM-3), oxyfunctionalizes alpha-pinene in an NADPH-dependent reaction to alpha-pinene oxide, verbenol, and myrtenol. We optimized the whole-cell biocatalyst by integrating a recombinant intracellular NADPH regeneration system through co-expression of a glucos...

journal_title：Applied microbiology and biotechnology

authors： Schewe H,Kaup BA,Schrader J

更新日期：2008-02-01 00:00:00

abstract：:Lipophilic extractives in wood and other lignocellulosic materials exert a negative impact in pulp and paper manufacturing causing the so-called pitch problems. In this work, the appropriateness of an enzymatic treatment using the laccase-mediator system for pitch biocontrol is evaluated. With this purpose, three pulp...

journal_title：Applied microbiology and biotechnology

authors： Gutiérrez A,del Río JC,Rencoret J,Ibarra D,Martínez AT

更新日期：2006-10-01 00:00:00

abstract：:Various techniques are used to adjust the flavors of foods and beverages to new market demands. Although synthetic flavoring chemicals are still widely used, flavors produced by biological methods (bioflavors) are now more and more requested by consumers, increasingly concerned with health and environmental problems c...

journal_title：Applied microbiology and biotechnology

authors： Vanderhaegen B,Neven H,Coghe S,Verstrepen KJ,Derdelinckx G,Verachtert H

更新日期：2003-08-01 00:00:00

abstract：:Human interleukin 6 (hIL-6) overproduced in Escherichia coli HB101 was found to partially retain the initiator methionine (Met) residue (Met-hIL-6). In order to remove the residual N-terminal Met in vivo, an attempt was made to express hIL-6 in aminopeptidase-P (Ap-P)-hyperproducing strains, since the N-terminus Met-P...

journal_title：Applied microbiology and biotechnology

authors： Yasueda H,Kikuchi Y,Kojima H,Nagase K

更新日期：1991-11-01 00:00:00

abstract：:The extrusion technology of blends formed by compounds with different physicochemical properties often results in new materials that present properties distinctive from its original individual constituents. Here, we report the use of melt extrusion of blends made from low-cost materials to produce a biodegradable foam...

journal_title：Applied microbiology and biotechnology

authors： Marcelino PR,Milani KM,Mali S,Santos OJ,de Oliveira AL

更新日期：2016-08-01 00:00:00

abstract：:Photosynthetic microorganisms produce relatively large amounts of physiologically active materials which stimulate the physiological activity of other organisms. In this study, mammalian HeLa cells were cultured in different culture media which were Dulbecco's modified Eagle medium (DMEM) with newborn calf serum (NCS)...

journal_title：Applied microbiology and biotechnology

authors： Lee HJ,Park JY,Yoo KS,Yoon J,Kim YH,Min J

更新日期：2013-11-01 00:00:00

abstract：:Butenyl-spinosyn produced by Saccharopolyspora pogona exhibits strong insecticidal activity and broad pesticidal spectrum. However, its synthetic level was low in the wild-type strain. At present, important functional genes involved in butenyl-spinosyn biosynthesis remain unknown, which leads to difficulty in efficien...

journal_title：Applied microbiology and biotechnology

authors： Rang J,Zhu Z,Li Y,Cao L,He H,Tang J,Hu J,Chen J,Hu S,Huang W,Yu Z,Ding X,Sun Y,Xie Q,Xia L

更新日期：2021-01-23 00:00:00

abstract：:Acrylic polymers (AP) are a diverse group of materials with broad applications, frequent use, and increasing demand. Some of the most used AP are polyacrylamide, polyacrylic acid, polymethyl methacrylates, and polyacrylonitrile. Although no information for the production of all AP types is published, data for the most...

journal_title：Applied microbiology and biotechnology

authors： Gaytán I,Burelo M,Loza-Tavera H

更新日期：2021-01-11 00:00:00

abstract：:Yeast artificial chromosomes (YAC) splitting technology was developed as a means to subclone any desired region of eukaryotic chromosomes from one YAC into new YACs. In the present study, the conventional YAC splitting technology was improved by incorporating PCR-mediated chromosome splitting technique and by adding a...

journal_title：Applied microbiology and biotechnology

authors： Kim Y,Sugiyama M,Yamagishi K,Kaneko Y,Fukui K,Kobayashi A,Harashima S

更新日期：2005-11-01 00:00:00

abstract：:A highly hydrophobic and non-toxic colloidal silica nanoparticle/polyvinyl butyral sol-gel composite membrane was prepared on a platinum wire electrode. With diphtheria-toxoid (D-Ag) as a model antigen and encapsulation of diphtheria antibody (D-Ab) in the composite architecture, this membrane could be used for reagen...

journal_title：Applied microbiology and biotechnology

authors： Wang FC,Yuan R,Chai YQ

更新日期：2006-10-01 00:00:00

abstract：:C-phycocyanin (C-PC) was extracted from fresh Spirulina platensis by deploying a species of non-pathogenic nitrogen-fixing bacteria, namely, Klebsiella pneumoniae. The algal slurry was neither washed nor centrifuged; the bacterial culture was poured into the slurry, the vessel sealed, and crude C-PC extracted after ab...

journal_title：Applied microbiology and biotechnology

authors： Zhu Y,Chen XB,Wang KB,Li YX,Bai KZ,Kuang TY,Ji HB

更新日期：2007-02-01 00:00:00

abstract：:N-acetyl glutamate kinase (NAGK) is a key enzyme in the synthesis of L-arginine that is inhibited by its end product L-arginine in Corynebacterium glutamicum (C. glutamicum). In this study, the potential binding sites of arginine and the residues essential for its inhibition were identified by homology modeling, inhib...

journal_title：Applied microbiology and biotechnology

authors： Huang Y,Zhang H,Tian H,Li C,Han S,Lin Y,Zheng S

更新日期：2015-09-01 00:00:00

abstract：:The TonB system functions in iron transport and has been identified in certain Gram-negative bacteria. Recently, we reported three TonB systems in the Aeromonas hydrophila Chinese epidemic strain NJ-35, but the functions of these systems have not been thoroughly elucidated to date. In this study, we investigated the r...

journal_title：Applied microbiology and biotechnology

authors： Dong Y,Geng J,Liu J,Pang M,Awan F,Lu C,Liu Y

更新日期：2019-05-01 00:00:00

abstract：:The membraneless bioelectrochemical reactor (Ml-BER) is useful for dark hydrogen fermentation. The effect of the electrochemical reaction on microorganisms in the Ml-BER was investigated using glucose as the substrate and compared with organisms in a membraneless non-bioelectrochemical reactor (Ml-NBER) and bioelectro...

journal_title：Applied microbiology and biotechnology

authors： Sasaki K,Morita M,Sasaki D,Ohmura N,Igarashi Y

更新日期：2013-08-01 00:00:00

abstract：:The production of biogas by anaerobic digestion (AD) of agricultural residues, organic wastes, animal excrements, municipal sludge, and energy crops has a firm place in sustainable energy production and bio-economy strategies. Focusing on the microbial community involved in biomass conversion offers the opportunity to...

journal_title：Applied microbiology and biotechnology

authors： Hassa J,Maus I,Off S,Pühler A,Scherer P,Klocke M,Schlüter A

更新日期：2018-06-01 00:00:00

abstract：:Currently, increasing attention cancer treatment has focused on molecularly targeted therapies and more recently on immunotherapies targeting immune checkpoints. However, even such advanced treatment may be ineffective. The reasons for this are sought, inter alia, in the human microbiome. In our intestines, there are ...

journal_title：Applied microbiology and biotechnology

authors： Grenda A,Krawczyk P

更新日期：2021-01-25 00:00:00

abstract：:Oil-producing fungus Mortierella alpina 1S-4 is an industrial strain. To determine its physiological properties and to clarify the biosynthetic pathways for polyunsaturated fatty acids, a transformation system for this fungus was established using a derivative of it, i.e., a ura5- mutant lacking orotate phosphoribosyl...

journal_title：Applied microbiology and biotechnology

authors： Takeno S,Sakuradani E,Murata S,Inohara-Ochiai M,Kawashima H,Ashikari T,Shimizu S

更新日期：2004-09-01 00:00:00

abstract：:The biofilm formation of Pseudomonas aeruginosa, an opportunistic human pathogen, is developed by cell-to-cell signaling, so-called quorum sensing (QS). To control the biofilm formation, we designed and synthesized new QS inhibitors of P. aeruginosa based on the structure of the previously known QS inhibitor, furanone...

journal_title：Applied microbiology and biotechnology

authors： Kim C,Kim J,Park HY,Park HJ,Lee JH,Kim CK,Yoon J

更新日期：2008-08-01 00:00:00

abstract：:Poly(lactate-co-3-hydroxybutyrate) (P(LA-co-3HB)) was previously produced from xylose in engineered Escherichia coli. The aim of this study was to increase the polymer productivity and LA fraction in P(LA-co-3HB) using two metabolic engineering approaches: (1) deletions of competing pathways to lactate production and ...

journal_title：Applied microbiology and biotechnology

authors： Nduko JM,Matsumoto K,Ooi T,Taguchi S

更新日期：2014-03-01 00:00:00

abstract：:Traditional temperature-sensitive systems use either heat shock (40-42 °C) or cold shock (15-23 °C) to induce gene expression at temperatures that are not the optimal temperature for host cell growth (37 °C). This impacts the overall productivity and yield by disturbing cell growth and cellular metabolism. Here, we ha...

journal_title：Applied microbiology and biotechnology

authors： Jiang X,Zhang H,Yang J,Liu M,Feng H,Liu X,Cao Y,Feng D,Xian M

更新日期：2013-06-01 00:00:00

abstract：:Xylose is, after glucose, the dominant sugar in agricultural wastes. In anaerobic environments, carbohydrates are converted into volatile fatty acids and alcohols. These can be used as building blocks in biotechnological or chemical processes, e.g., to produce bioplastics. In this study, xylose fermentation by mixed m...

journal_title：Applied microbiology and biotechnology

authors： Temudo MF,Mato T,Kleerebezem R,van Loosdrecht MC

更新日期：2009-02-01 00:00:00

abstract：:Functional genomics of filamentous fungi has gradually uncovered gene information for constructing 'cell factories' and controlling pathogens. Available gene manipulation methods of filamentous fungi include random integration methods, gene targeting technology, gene editing with artificial nucleases and RNA technolog...

journal_title：Applied microbiology and biotechnology

authors： Wang S,Chen H,Tang X,Zhang H,Chen W,Chen YQ

更新日期：2017-11-01 00:00:00

abstract：:The quorum sensing signalling molecules 2-heptyl-3-hydroxy-4(1H)-quinolone, termed the "Pseudomonas quinolone signal" (PQS), and 2-heptyl-4(1H)-quinolone (HHQ) play an important role in the control of virulence gene expression in Pseudomonas aeruginosa. To construct a bioreporter for the specific and sensitive detecti...

journal_title：Applied microbiology and biotechnology

authors： Müller C,Fetzner S

更新日期：2013-01-01 00:00:00

abstract：:The pathogenicity of Salmonella Typhimurium, a foodborne pathogen, is mainly attributed to its ability to form biofilm on food contact surfaces. ε-polylysine, a polymer of positively charged lysine, is reported to inhibit biofilm formation of both gram-positive and gram-negative bacteria. To elucidate the mechanism un...

journal_title：Applied microbiology and biotechnology

authors： Shen C,Islam MT,Masuda Y,Honjoh KI,Miyamoto T

更新日期：2020-06-01 00:00:00

abstract：:Cell growth needs to be monitored in biological studies and bioprocess optimization. In special circumstances, such as microbial fermentations in media containing insoluble particles, accurate cell growth quantification is a challenge with current methods. Only the Burton method is applicable in such circumstances. Th...

journal_title：Applied microbiology and biotechnology

authors： Zhao Y,Xiang S,Dai X,Yang K

更新日期：2013-06-01 00:00:00

abstract：:The objective of this study was to develop a non-virulent simulant to replace several virulent organisms during the development of detection and identification methods for biological threat agents. We identified and selected specific genes to detect Yersinia pestis, Francisella tularensis, Burkholderia mallei, Burkhol...

journal_title：Applied microbiology and biotechnology

authors： Carrera M,Sagripanti JL

更新日期：2009-01-01 00:00:00

abstract：:The alpha-amylase gene (amy) from Streptomyces griseus IMRU 3570 and the beta-galactosidase gene (lac) from S. lividans were subcloned into Brevibacterium lactofermentum or B. lactofermentum/Escherichia coli shuttle vectors. The amy gene was not expressed in B. lactofermentum from its own promoter but was efficiently ...

journal_title：Applied microbiology and biotechnology

authors： Cadenas RF,Gil JA,Martín JF

更新日期：1992-12-01 00:00:00