当前位置： SCI文献检索 > APPLIED MICROBIOLOGY AND BIOTECHNOLOGY期刊下所有文献 > Activity and characterization of mixed organic compounds extracted from Rhodobacter sphaeroides as alternative materials to serum for mammalian cell growth.

Activity and characterization of mixed organic compounds extracted from Rhodobacter sphaeroides as alternative materials to serum for mammalian cell growth.

Abstract：

:Photosynthetic microorganisms produce relatively large amounts of physiologically active materials which stimulate the physiological activity of other organisms. In this study, mammalian HeLa cells were cultured in different culture media which were Dulbecco's modified Eagle medium (DMEM) with newborn calf serum (NCS), and DMEM including different types of physiologically activating compounds (PACs) extracted from Rhodobacter sphaeroides grown under various culture conditions. R. sphaeroides was grown under the following five different culture conditions: anaerobically in the light, anaerobically in the dark and treated with dimethyl sulfoxide, aerobically in the dark for 48 h, in the light for 48 h, and in the light for 24 h and changed after previous culturing in the dark for 24 h. The growth of HeLa cell was measured by cell counting using a hemocytometer, and the fluorescent intensities of cellular lysosomes were measured to check the level of cellular stress caused by adding PACs. The growth of HeLa cells cultured in DMEM with PACs extracted from R. sphaeroides aerobically grown under dark conditions was enhanced compared to that of cells grown with NCS. We also found that a high concentration of pigments such as bacteriochlorophylls and carotenoids and a high concentration of arginine produced by R. sphaeroides aerobically grown in the dark were implicated in increased growth of the HeLa cells. Therefore, our results suggest that PACs extracted from R. sphaeroides aerobically cultured in dark conditions can enhance the physiological activity of mammalian cells and serve as nontoxic and bioavailable materials.

journal_name

Appl Microbiol Biotechnol

journal_title

Applied microbiology and biotechnology

authors

Lee HJ,Park JY,Yoo KS,Yoon J,Kim YH,Min J

doi

10.1007/s00253-012-4653-4

subject

Has Abstract

pub_date

2013-11-01 00:00:00

pages

9561-7

issue

21

eissn

0175-7598

issn

1432-0614

journal_volume

97

pub_type

杂志文章

相关文献

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY文献大全
  • A lab-scale model system for cocoa bean fermentation.

    abstract：:Lab-scale systems modelling the spontaneous cocoa bean fermentation process are useful tools to research the influence of process parameters on the fermentation and the final bean quality. In this study in Honduras, a 1-kg lab-scale fermentation (LS-F) was compared to a 300-kg on-farm fermentation (OF-F) in a multipha...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-018-8835-6

    authors： Romanens E,Näf R,Lobmaier T,Pedan V,Leischtfeld SF,Meile L,Schwenninger SM

    更新日期：2018-04-01 00:00:00

  • Effect of cysteine desulfhydrase gene disruption on L-cysteine overproduction in Escherichia coli.

    abstract：:In Escherichia coli, the enzyme called cysteine desulfhydrase (CD), which is responsible for L-cysteine degradation, was investigated by native-PAGE and CD activity staining of crude cell extracts. Analyses with gene-disrupted mutants showed that CD activity resulted from two enzymes: tryptophanase (TNase) encoded by ...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-003-1262-2

    authors： Awano N,Wada M,Kohdoh A,Oikawa T,Takagi H,Nakamori S

    更新日期：2003-08-01 00:00:00

  • Production of L-phenylacetylcarbinol by microbial transformation in polyethylene glycol-induced cloud point system.

    abstract：:Microbial transformation of benzaldehyde into L: -phenylacetylcarbinol by whole cell Saccharomyces cerevisiae has been carried out in a novel polyethylene glycol (PEG)-induced cloud point system. The system is composed of 80 g PEG 20,000, 75 ml Triton X-100, 20 g peptone, 10 g yeast extract, 25 g glucose, 1 g MgSO(4)....

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-007-1304-2

    authors： Zhang W,Wang Z,Li W,Zhuang B,Qi H

    更新日期：2008-02-01 00:00:00

  • Characterization of the metabolic shift of Saccharomyces bayanus var. uvarum by continuous aerobic culture.

    abstract：:Saccharomyces bayanus, being of interest for wine-making, is not as well known as S. cerevisiae and, due to many changes in the yeast classification, accurate data concerning its metabolic activity are difficult to find. In order to produce this yeast as an active dry yeast to be used as a starter in wine-making, its ...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-003-1288-5

    authors： Serra A,Strehaiano P,Taillandier P

    更新日期：2003-10-01 00:00:00

  • Use of uridine auxotrophy (ura3) for markerless transformation of the mycoinsecticide Beauveria bassiana.

    abstract：:Genetic engineering offers a practical route for enhancing the insect biological control potential of entomopathogenic fungi such as Beauveria bassiana. To date, however, such efforts have relied upon transformation protocols that utilize antibiotic or herbicidal resistance markers as selection agents for the introduc...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-012-4426-0

    authors： Ying SH,Feng MG,Keyhani NO

    更新日期：2013-04-01 00:00:00

  • Diallyl disulfide from garlic oil inhibits Pseudomonas aeruginosa virulence factors by inactivating key quorum sensing genes.

    abstract：:Garlic oil can disrupt the quorum sensing (QS) pathways of the opportunistic pathogen Pseudomonas aeruginosa; however, the underlying mechanisms for this effect are unclear. Diallyl disulfide (DADS) is one of the most abundant sulfur-containing compounds in garlic oil. This study investigated the effects of DADS on th...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-018-9175-2

    authors： Li WR,Ma YK,Shi QS,Xie XB,Sun TL,Peng H,Huang XM

    更新日期：2018-09-01 00:00:00

  • Cumulative improvements of thermostability and pH-activity profile of Aspergillus niger PhyA phytase by site-directed mutagenesis.

    abstract：:Aspergillus niger phytase (PhyA) has been used as a feed supplement to reduce manure phosphorus excretion of swine and poultry but lacks sufficient thermostability for feed pelleting and appropriate pH-activity profile for phytate hydrolysis in the stomach of animals. Previously, a thermostable mutant PhyA18 and two p...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-007-1239-7

    authors： Zhang W,Lei XG

    更新日期：2008-01-01 00:00:00

  • Carbamoylases: characteristics and applications in biotechnological processes.

    abstract：:Enzymatic kinetic resolution is a widely used biotechnological tool for the production of enantiomerically pure/enriched compounds. This technique takes advantage of the enantioselectivity or enantiospecificity of an enzyme for one of the enantiomers of a racemic substrate to isolate the desired isomer. N-Carbamoyl-D-...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章,评审

    doi：10.1007/s00253-009-2250-y

    authors： Martínez-Rodríguez S,Martínez-Gómez AI,Rodríguez-Vico F,Clemente-Jiménez JM,Las Heras-Vázquez FJ

    更新日期：2010-01-01 00:00:00

  • Current advances in Phellinus sensu lato: medicinal species, functions, metabolites and mechanisms.

    abstract：:Twenty-six species of Phellinus sensu lato, reported as medicinal mushrooms, are enumerated in this review. The species' names were checked and revised according to contemporary taxonomy and the latest version of the International Code of Botanical Nomenclature (Vienna Code); two misapplied names of Phellinus baumii P...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章,评审

    doi：10.1007/s00253-010-2711-3

    authors： Dai YC,Zhou LW,Cui BK,Chen YQ,Decock C

    更新日期：2010-08-01 00:00:00

  • 1,3-Propanediol production in a two-step process fermentation from renewable feedstock.

    abstract：:In this work, the production of 1,3-propanediol from glucose and molasses was studied in a two-step process using two recombinant microorganisms. The first step of the process is the conversion of glucose or other sugar into glycerol by the metabolic engineered Saccharomyces cerevisiae strain HC42 adapted to high (>20...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-011-3369-1

    authors： Mendes FS,González-Pajuelo M,Cordier H,François JM,Vasconcelos I

    更新日期：2011-11-01 00:00:00

  • Profile of bacterial communities in South African mine-water samples using Illumina next-generation sequencing platform.

    abstract：:Mine water is an example of an extreme environment that contains a large number of diverse and specific bacteria. It is imperative to gain an understanding of these bacterial communities in order to develop effective strategies for the bioremediation of polluted aquatic systems. In this study, the high-throughput sequ...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-014-6213-6

    authors： Keshri J,Mankazana BB,Momba MN

    更新日期：2015-04-01 00:00:00

  • Behaviour of dehydrated baker's yeast during reduction reactions in a biphasic medium.

    abstract：:The behaviour of dry baker's yeast ( Saccharomyces cerevisiae type II, Sigma) used as biocatalyst without preliminary growth for the synthesis of 2-heptanol from 2-heptanone in a biphasic system is presented. Cells undergo intracellular trehalose consumption with a stoichiometric ethanol production during the first 15...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-003-1500-7

    authors： Cappaert L,Larroche C

    更新日期：2004-06-01 00:00:00

  • Kinetic studies on autohydrogenotrophic growth of Ralstonia eutropha with nitrate as terminal electron acceptor.

    abstract：:Autohydrogenotrophic batch growth of Ralstonia eutropha H16 was studied in a stirred-tank reactor with nitrate and nitrite as terminal electron acceptors and the sole limiting substrates. Assuming product inhibition by nitrite, saturation kinetics with the two limiting substrates and a simple switching function, which...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-007-0983-z

    authors： Tiemeyer A,Link H,Weuster-Botz D

    更新日期：2007-08-01 00:00:00

  • Lactobacillus reuteri CRL 1101 highly produces mannitol from sugarcane molasses as carbon source.

    abstract：:Mannitol is a natural polyol extensively used in the food industry as low-calorie sugar being applicable for diabetic food products. We aimed to evaluate mannitol production by Lactobacillus reuteri CRL 1101 using sugarcane molasses as low-cost energy source. Mannitol formation was studied in free-pH batch cultures us...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-012-3945-z

    authors： Ortiz ME,Fornaguera MJ,Raya RR,Mozzi F

    更新日期：2012-08-01 00:00:00

  • Vinyl ketone reduction by three distinct Gluconobacter oxydans 621H enzymes.

    abstract：:Three cytosolic NADPH-dependent flavin-associated proteins (Gox2107, Gox0502, and Gox2684) from Gluconobacter oxydans 621H were overproduced in Escherichia coli, and the recombinant enzymes were purified and characterized. Apparent native molecular masses of 65.2, 78.2, and 78.4 kDa were observed for Gox2107, Gox0502,...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-008-1600-5

    authors： Schweiger P,Gross H,Wesener S,Deppenmeier U

    更新日期：2008-10-01 00:00:00

  • Development of sandwich ELISAs that can distinguish different types of coxsackievirus A16 viral particles.

    abstract：:Coxsackievirus A16 (CA16) is one of the major causative agents of hand, foot, and mouth disease (HFMD). No CA16 vaccine candidates have progressed to clinical trials so far. Immunogenicity studies indicated that different CA16 particles have much influence on the efficacy of a candidate vaccine. However, there are sti...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-016-7296-z

    authors： Ye X,Yang L,Jia J,Han J,Li S,Liu Y,Xu L,Zhao H,Chen Y,Li Y,Cheng T,Xia N

    更新日期：2016-03-01 00:00:00

  • The use of extracellular enzymes from Streptomyces albus ATCC 3005 for the bleaching of eucalyptus kraft pulp.

    abstract：:The suitability of culture supernatant from Streptomyces albus ATCC 3005 for use in the biobleaching of eucalyptus kraft pulp was investigated. S. albus was found to grow on a minimal salts medium containing oat spelts xylan and yeast extract as the main carbon and nitrogen sources, respectively. Maximal extracellular...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s002530100740

    authors： Antonopoulos VT,Hernandez M,Arias ME,Mavrakos E,Ball AS

    更新日期：2001-10-01 00:00:00

  • Cold adaptation of a mesophilic serine protease, subtilisin, by in vitro random mutagenesis.

    abstract：:Artificial cold adaptation of a mesophilic protease, subtilisin BPN' was attempted by means of random mutagenesis of its entire gene coupled with screening of cleared-zone-forming colonies on skim-milk plates at a low temperature. Out of sixty clones screened at 10 degrees C, one mutant enzyme (termed M-15) was found ...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s002530050886

    authors： Kano H,Taguchi S,Momose H

    更新日期：1997-01-01 00:00:00

  • The diversity and distribution of anammox bacteria in the marine aquaculture zones.

    abstract：:The accumulation of toxic inorganic nitrogen is one of the major water quality problems in intensive aquaculture systems, thus the N removal in aquaculture systems is an important issue for the sustainable development of aquaculture. To understand one of the major microbial N removal processes, anaerobic ammonium oxid...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-016-7690-6

    authors： Li M,Gu JD

    更新日期：2016-10-01 00:00:00

  • Biology, dynamics, and applications of transposable elements in basidiomycete fungi.

    abstract：:The phylum Basidiomycota includes filamentous fungi and yeast species with different ecological and genomic characteristics. Transposable elements (TEs) are abundant components of most eukaryotic genomes, and their transition from being genomic parasites to key drivers of genomic architecture, functionality, and evolu...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章,评审

    doi：10.1007/s00253-017-8097-8

    authors： Castanera R,Borgognone A,Pisabarro AG,Ramírez L

    更新日期：2017-02-01 00:00:00

  • Isolation and characterization of an antifungal peptide with antiproliferative activity from seeds of Phaseolus vulgaris cv. 'Spotted Bean'.

    abstract：:A 7.3-kDa antifungal peptide with an N-terminal sequence exhibiting remarkable homology to defensins from other leguminous plants was isolated from Phaseolus vulgaris cv. 'Spotted Bean'. The isolation procedure involved ion exchange chromatography on O-diethylaminoethyl (DEAE) cellulose, affinity chromatography on Aff...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-006-0650-9

    authors： Wang HX,Ng TB

    更新日期：2007-02-01 00:00:00

  • Structural and physicochemical properties of polar lipids from thermophilic archaea.

    abstract：:The essential general features required for lipid membranes of extremophilic archaea to fulfill biological functions are that they are in the liquid crystalline phase and have extremely low permeability of solutes that is much less temperature sensitive due to a lack of lipid-phase transition and highly branched isopr...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章,评审

    doi：10.1007/s00253-009-2102-9

    authors： Ulrih NP,Gmajner D,Raspor P

    更新日期：2009-08-01 00:00:00

  • A promiscuous prenyltransferase from Aspergillus oryzae catalyses C-prenylations of hydroxynaphthalenes in the presence of different prenyl donors.

    abstract：:Prenyltransferases of the dimethylallyltryptophan synthase (DMATS) superfamily are involved in the biosynthesis of secondary metabolites and show broad substrate specificity towards their aromatic substrates with a high regioselectivity for the prenylation reactions. Most members of this superfamily accepted as prenyl...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-014-5509-x

    authors： Pockrandt D,Sack C,Kosiol T,Li SM

    更新日期：2014-06-01 00:00:00

  • Genome engineering in actinomycetes using site-specific recombinases.

    abstract：:The rational modification of the actinomycetes genomes has a variety of applications in research, medicine, and biotechnology. The use of site-specific recombinases allows generation of multiple mutations, large DNA deletions, integrations, and inversions and may lead to significant progress in all of these fields. De...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章,评审

    doi：10.1007/s00253-013-4866-1

    authors： Myronovskyi M,Luzhetskyy A

    更新日期：2013-06-01 00:00:00

  • Novel nitrifiers and comammox in a full-scale hybrid biofilm and activated sludge reactor revealed by metagenomic approach.

    abstract：:Biofilms are widely used in wastewater treatment for their particular enhancement of nitrogen removal and other significant advantages. In this study, the diversity and potential functions of nitrogen removal bacteria in suspended activated sludge (AS) and biofilm of a full-scale hybrid reactor were uncovered by metag...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-016-7655-9

    authors： Chao Y,Mao Y,Yu K,Zhang T

    更新日期：2016-09-01 00:00:00

  • Stress tolerance phenotype of industrial yeast: industrial cases, cellular changes, and improvement strategies.

    abstract：:Yeast is widely used in the baking, biocontrol, brewing, and bio manufacturing industries. In the baking industry alone, around two million tons of yeast are consumed worldwide every year. While yeast brings delicious and healthy lives to humans, we find that stress resistance of yeast is essential for the development...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章,评审

    doi：10.1007/s00253-019-09993-8

    authors： Qiu X,Zhang J,Zhou J,Fang Z,Zhu Z,Li J,Du G

    更新日期：2019-08-01 00:00:00

  • On-line infrared spectroscopy for bioprocess monitoring.

    abstract：:One of the major aims of bioprocess engineering is the real-time monitoring of important process variables. This is the basis of precise process control and is essential for high productivity as well as the exact documentation of the overall production process. Infrared spectroscopy is a powerful analytical technique ...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章,评审

    doi：10.1007/s00253-010-2743-8

    authors： Landgrebe D,Haake C,Höpfner T,Beutel S,Hitzmann B,Scheper T,Rhiel M,Reardon KF

    更新日期：2010-09-01 00:00:00

  • An L-arabinose isomerase from Acidothermus cellulolytics ATCC 43068: cloning, expression, purification, and characterization.

    abstract：:The araA gene encoding an L-arabinose isomerase (L-AI) from the acido-thermophilic bacterium Acidothermus cellulolytics ATCC 43068 was cloned and overexpressed in Escherichia coli. The open reading frame of the L-AI consisted of 1,503 nucleotides encoding 501 amino acid residues. The recombinant L-AI was purified to h...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-009-2322-z

    authors： Cheng L,Mu W,Zhang T,Jiang B

    更新日期：2010-04-01 00:00:00

  • In vitro propagation and cell cultures of memory tonic herb Evolvulus alsinoides: a best source for elicited production of scopoletin.

    abstract：:Evolvulus alsinoides L. is used for preparation of 'Shankhapushpi', an important popular ayurvedic drug that contributes considerably to the improvement of memory power. The improvement is attributed to the presence of furanocoumarin scopoletin, a metabolite with a wide range of biological activities. This report desc...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-015-7153-5

    authors： Naikawadi VB,Ahire ML,Lahiri A,Nikam TD

    更新日期：2016-04-01 00:00:00

  • Biosynthesis of mono-acylated mannosylerythritol lipid in an acyltransferase gene-disrupted mutant of Pseudozyma tsukubaensis.

    abstract：:The basidiomycetous yeast genus Pseudozyma produce large amounts of mannosylerythritol lipids (MELs), which are biosurfactants. A few Pseudozyma strains produce mono-acylated MEL as a minor compound using excess glucose as the sole carbon source. Mono-acylated MEL shows higher hydrophilicity than di-acylated MEL and h...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-017-8698-2

    authors： Saika A,Utashima Y,Koike H,Yamamoto S,Kishimoto T,Fukuoka T,Morita T

    更新日期：2018-02-01 00:00:00