Biology, dynamics, and applications of transposable elements in basidiomycete fungi.

abstract：:In oyster ponds, the marine diatom Haslea ostrearia synthesises and excretes a hydrosoluble pigment of commercial interest called marennin. During the benthic stage, when algal cells are naturally immobilised in their own polysaccharides, marennin production is higher. To optimise this production, axenic cultures of H...

journal_title：Applied microbiology and biotechnology

authors： Lebeau T,Gaudin P,Moan R,Robert JM

更新日期：2002-07-01 00:00:00

abstract：:The first two genes of the threonine pathway, ask and asd, were cloned and sequenced from the aminoethoxyvinylglycine-producing Streptomyces sp. NRRL 5331. The two genes are organized in a bicistronic operon. ask, encoding the apartokinase (ASK), is located upstream from asd. The presence of a ribosome-binding site wi...

journal_title：Applied microbiology and biotechnology

authors： Cuadrado Y,Fernández M,Recio E,Aparicio JF,Martín JF

更新日期：2004-04-01 00:00:00

abstract：:We have constructed a chimeric protein composed of the receptor binding and membrane translocation domains of Pseudomonas exotoxin A (PE) with the outer membrane proteins I and F, together designated as PEIF. The potential of PEIF as a vaccine against Pseudomonas infection was evaluated in BALB/c mice and New Zealand ...

journal_title：Applied microbiology and biotechnology

authors： Chen TY,Shang HF,Chen TL,Lin CP,Hui CF,Hwang J

更新日期：1999-10-01 00:00:00

abstract：:The transport of infectious and biological material is regulated by a number of international organizations. This mini-review has been compiled to increase awareness within the scientific community of problems caused by differences in terminology (such as infectious materials/substances, biological products, diagnosti...

journal_title：Applied microbiology and biotechnology

authors： Lelieveld HL,Bachmayer H,Boon B,Bennett A,Brunius G,Bürki K,Cantley M,Collard JM,Collins CH,Crooy P,Doblhoff-Dier O,Dubakiene R,Economidis I,Elmqvist A,Frontali-Botti C,Havenaar R,Haymerle H,Käppeli O,Leaver G,Lex M

更新日期：1997-08-01 00:00:00

abstract：:Porcine parvovirus (PPV) virus-like particles (VLPs) constitute a potential vaccine for prevention of parvovirus-induced reproductive failure in gilts. Here we report the development of a large scale (25 l) production process for PPV-VLPs with baculovirus-infected insect cells. A low multiplicity of infection (MOI) st...

journal_title：Applied microbiology and biotechnology

authors： Maranga L,Rueda P,Antonis AF,Vela C,Langeveld JP,Casal JI,Carrondo MJ

更新日期：2002-06-01 00:00:00

abstract：:Toluene dioxygenase (TDO) catalyzes asymmetric cis-dihydroxylation of aromatic compounds. To achieve high efficient biotransformation of benzene to benzene cis-diols, Pseudomonas putida KT2442, Pseudomonas stutzeri 1317, and Aeromonas hydrophila 4AK4 were used as hosts to express TDO gene tod. Plasmid pSPM01, a deriva...

journal_title：Applied microbiology and biotechnology

authors： Ouyang SP,Sun SY,Liu Q,Chen J,Chen GQ

更新日期：2007-02-01 00:00:00

abstract：:This study was aimed to investigate the effect of a single-chain fragment variable antibody of connective tissue growth factor (anti-CTGF scFv) against the differentiation of fibroblast into myofibroblast. The scFv antibody was firstly expressed in Escherichia coli cells and was then purified by affinity chromatograph...

journal_title：Applied microbiology and biotechnology

authors： Wu G,Wang X,Deng X,Wu P,Xue X,Yan X,Wang X

更新日期：2012-03-01 00:00:00

abstract：:Only two polyethylene glycol terephthalate (PET)-degrading enzymes have been reported, and their mechanism for the biochemical degradation of PET remains unclear. To identify a novel PET-degrading enzyme, a putative cutinase gene (cut190) was cloned from the thermophile Saccharomonospora viridis AHK190 and expressed i...

journal_title：Applied microbiology and biotechnology

authors： Kawai F,Oda M,Tamashiro T,Waku T,Tanaka N,Yamamoto M,Mizushima H,Miyakawa T,Tanokura M

更新日期：2014-12-01 00:00:00

abstract：:Corynebacterium glutamicum, a gram-positive soil bacterium, has been regarded as an aerobe because its growth by fermentative catabolism or by anaerobic respiration has, to this date, not been demonstrated. In this study, we report on the anaerobic growth of C. glutamicum in the presence of nitrate as a terminal elect...

journal_title：Applied microbiology and biotechnology

authors： Nishimura T,Vertès AA,Shinoda Y,Inui M,Yukawa H

更新日期：2007-06-01 00:00:00

abstract：:Epilactose (4-O-β-D-galactopyranosyl-D-mannose), an epimer of lactose, is a rare disaccharide existing extremely small quantities in heat-treated milk, in which epilactose is produced by non-enzymatic catalysis from lactose. This disaccharide is a kind of non-digestible carbohydrate, has a good prebiotic effect, and p...

journal_title：Applied microbiology and biotechnology

authors： Mu W,Li Q,Fan C,Zhou C,Jiang B

更新日期：2013-03-01 00:00:00

abstract：:The adhesion properties of 14 Streptococcus salivarius strains to mucus (HT29-MTX) and non-mucus secreting (Caco-2/TC7) human intestinal epithelial cells were investigated. Ability to adhere to these two eukaryotic cell lines greatly differs between strains. The presence of mucus played a major factor in adhesion, lik...

journal_title：Applied microbiology and biotechnology

authors： Chaffanel F,Charron-Bourgoin F,Soligot C,Kebouchi M,Bertin S,Payot S,Le Roux Y,Leblond-Bourget N

更新日期：2018-03-01 00:00:00

abstract：:The present study is focused on systematic process and kinetic investigation of hyaluronic acid (HA) production strategy unraveling the role of dissolved oxygen (DO) and N-acetyl glucosamine (GlcNAc) towards the enhancement of HA titer and its molecular weight. Maintaining excess DO levels (10-40% DO) through DO-stat ...

journal_title：Applied microbiology and biotechnology

authors： Mohan N,Tadi SRR,Pavan SS,Sivaprakasam S

更新日期：2020-04-01 00:00:00

abstract：:Blowfly (Diptera: Calliphoridae) species Lucilia sericata (Meigen) and related species Lucilia cuprina (Wiedmann) are important agricultural pests, assist in forensic fields and also have a therapeutic role in medicine. Both species (though predominantly L. sericata) are utilised in a clinical setting for maggot debri...

journal_title：Applied microbiology and biotechnology

authors： Gasz NE,Geary MJ,Doggett SL,Harvey ML

更新日期：2021-01-07 00:00:00

abstract：:The impact of glucose on glycerol metabolism, especially on 3-hydroxypropionaldehyde (3-HPA) accumulation by resting cells of Lactobacillus reuteri has been investigated. Two systems were used in the study: MRS(-) (modified MRS - omitting glucose, acetate and Tween 80) and distilled water (H(2)O). In MRS(-), addition ...

journal_title：Applied microbiology and biotechnology

authors： Lüthi-Peng Q,Dileme FB,Puhan Z

更新日期：2002-07-01 00:00:00

abstract：:Leavening ability in sweet dough is required for the commercial applications of baker's yeast. This property depends on many factors, such as glycolytic activity, sucrase activity, and osmotolerance. This study explored the importance of sucrase level on the leavening ability of baker's yeast in sweet dough. Furthermo...

journal_title：Applied microbiology and biotechnology

authors： Zhang CY,Lin X,Feng B,Liu XE,Bai XW,Xu J,Pi L,Xiao DG

更新日期：2016-07-01 00:00:00

abstract：:Aspergillus niger phytase (PhyA) has been used as a feed supplement to reduce manure phosphorus excretion of swine and poultry but lacks sufficient thermostability for feed pelleting and appropriate pH-activity profile for phytate hydrolysis in the stomach of animals. Previously, a thermostable mutant PhyA18 and two p...

journal_title：Applied microbiology and biotechnology

authors： Zhang W,Lei XG

更新日期：2008-01-01 00:00:00

abstract：:Microbial sulfate reduction with acetate as carbon source and electron donor was investigated at salinity levels between 0.53 and 1.48%. The experiment was carried out in a 2.3-1 upflow anaerobic sludge blanket reactor inoculated with granular methanogenic sludge. A pH of 8.3, a temperature of 32 +/- 1 degrees C and a...

journal_title：Applied microbiology and biotechnology

authors： Muthumbi W,Boon N,Boterdaele R,De Vreese I,Top EM,Verstraete W

更新日期：2001-06-01 00:00:00

abstract：:Metabolic stress is a phenomenon often discussed in conjunction with recombinant protein production in Escherichia coli. This investigation shows how heterologous protein production and the presence of host cell proteases is related to: (1) Isopropyl-beta- D-thiogalactopyranoside (IPTG) induction, (2) cell-mass concen...

journal_title：Applied microbiology and biotechnology

authors： Ramchuran SO,Nordberg Karlsson E,Velut S,De Maré L,Hagander P,Holst O

更新日期：2002-12-01 00:00:00

abstract：:Effects of select electron mediators [9,10-anthraquinone-2,6-disulfonic acid disodium salt (AQDS), safranine O, resazurin, methylene blue, and humic acids] on metabolic end-products and current production from cellulose digestion by Clostridium cellulolyticum in microbial fuel cells (MFCs) were studied using capillary...

journal_title：Applied microbiology and biotechnology

authors： Sund CJ,McMasters S,Crittenden SR,Harrell LE,Sumner JJ

更新日期：2007-09-01 00:00:00

abstract：:Chitin was purified from Penaeus monodon and Crangon crangon shells using a two-stage fermentation process with anaerobic deproteination followed by decalcification through homofermentative lactic acid fermentation. Deproteinating enrichment cultures from sewage sludge and ground meat (GM) were used with a proteolytic...

journal_title：Applied microbiology and biotechnology

authors： Xu Y,Gallert C,Winter J

更新日期：2008-06-01 00:00:00

abstract：:Pneumococcal surface protein A (PspA) is essential for Streptococcus pneumoniae virulence and its use either as a novel pneumococcal vaccine or as carrier in a conjugate vaccine would improve the protection and the coverage of the vaccine. Within this context, the development of scalable production and purification pr...

journal_title：Applied microbiology and biotechnology

authors： Carvalho RJ,Cabrera-Crespo J,Tanizaki MM,Gonçalves VM

更新日期：2012-05-01 00:00:00

abstract：:Physiological heterogeneity constitutes a critical parameter in biotechnological systems since both metabolite yield and productivity are often hampered by the presence of undesired physiological cell subpopulations. In the present study, the physiological status and functionality of Pseudomonas taetrolens cells were ...

journal_title：Applied microbiology and biotechnology

authors： Alonso S,Rendueles M,Díaz M

更新日期：2012-12-01 00:00:00

abstract：:Biotin (Vitamin B7) is a critical enzyme co-factor in metabolic pathways important for bacterial survival. Biotin is obtained either from the environment or by de novo synthesis, with some bacteria capable of both. In certain species, the bifunctional protein BirA plays a key role in biotin homeostasis as it regulates...

journal_title：Applied microbiology and biotechnology

authors： Satiaputra J,Eijkelkamp BA,McDevitt CA,Shearwin KE,Booker GW,Polyak SW

更新日期：2018-04-01 00:00:00

abstract：:Linkages among bioreactor operation and performance and microbial community structure were investigated for a fixed-bed biofilm system designed to remove perchlorate from drinking water. Perchlorate removal was monitored to evaluate reactor performance during and after the frequency and intensity of the backwash proce...

journal_title：Applied microbiology and biotechnology

authors： Li X,Yuen W,Morgenroth E,Raskin L

更新日期：2012-11-01 00:00:00

abstract：:Bacteriophages are bacterial viruses that infect the host after successful receptor recognition and adsorption to the cell surface. The irreversible adherence followed by genome material ejection into host cell cytoplasm must be preceded by the passage of diverse carbohydrate barriers such as capsule polysaccharides (...

journal_title：Applied microbiology and biotechnology

authors： Latka A,Maciejewska B,Majkowska-Skrobek G,Briers Y,Drulis-Kawa Z

更新日期：2017-04-01 00:00:00

abstract：:In Escherichia coli, the enzyme called cysteine desulfhydrase (CD), which is responsible for L-cysteine degradation, was investigated by native-PAGE and CD activity staining of crude cell extracts. Analyses with gene-disrupted mutants showed that CD activity resulted from two enzymes: tryptophanase (TNase) encoded by ...

journal_title：Applied microbiology and biotechnology

authors： Awano N,Wada M,Kohdoh A,Oikawa T,Takagi H,Nakamori S

更新日期：2003-08-01 00:00:00

abstract：:A 7.3-kDa antifungal peptide with an N-terminal sequence exhibiting remarkable homology to defensins from other leguminous plants was isolated from Phaseolus vulgaris cv. 'Spotted Bean'. The isolation procedure involved ion exchange chromatography on O-diethylaminoethyl (DEAE) cellulose, affinity chromatography on Aff...

journal_title：Applied microbiology and biotechnology

authors： Wang HX,Ng TB

更新日期：2007-02-01 00:00:00

abstract：:Time-resolved analysis assays of receptor-ligand interactions are fundamental in basic research and drug discovery. Adequate methods are well developed for the analysis of recombinant proteins such as antibody-antigen interactions. However, assays for time-resolved ligand-binding processes on living cells are still ra...

journal_title：Applied microbiology and biotechnology

authors： Encarnação JC,Schulte T,Achour A,Björkelund H,Andersson K

更新日期：2018-05-01 00:00:00

abstract：:DNA microarrays have found widespread use as a flexible tool to investigate bacterial metabolism. Their main advantage is the comprehensive data they produce on the transcriptional response of the whole genome to an environmental or genetic stimulus. This allows the microbiologist to monitor metabolism and to define s...

journal_title：Applied microbiology and biotechnology

authors： Ehrenreich A

更新日期：2006-11-01 00:00:00

abstract：:The ability to produce (R)- or (S)-beta-phenylalanine ethyl ester (3-amino-3-phenylpropionic acid ethyl ester, BPAE) from racemic BPAE through stereoselective hydrolysis was screened for in BPAE-assimilating microorganisms. Sphingobacterium sp. 238C5 and Arthrobacter sp. 219D2 were found to be potential catalysts for ...

journal_title：Applied microbiology and biotechnology

authors： Ogawa J,Mano J,Shimizu S

更新日期：2006-05-01 00:00:00