当前位置： SCI文献检索 > APPLIED MICROBIOLOGY AND BIOTECHNOLOGY期刊下所有文献 > A novel single-chain Fv antibody for connective tissue growth factor against the differentiation of fibroblast into myofibroblast.

A novel single-chain Fv antibody for connective tissue growth factor against the differentiation of fibroblast into myofibroblast.

Abstract：

:This study was aimed to investigate the effect of a single-chain fragment variable antibody of connective tissue growth factor (anti-CTGF scFv) against the differentiation of fibroblast into myofibroblast. The scFv antibody was firstly expressed in Escherichia coli cells and was then purified by affinity chromatography. The yield scFv protein reached a purity over 95% after purification. Immunoreactivity assay demonstrated that scFv possessed a special affinity toward CTGF. RT-PCR, western blot, and immunofluorescence experiments showed that increased expression of α-smooth muscle actin induced by TGF-β1 could be suppressed by this scFv antibody through inhibiting the phosphorylation of Akt.

journal_name

Appl Microbiol Biotechnol

journal_title

Applied microbiology and biotechnology

authors

Wu G,Wang X,Deng X,Wu P,Xue X,Yan X,Wang X

doi

10.1007/s00253-011-3755-8

subject

Has Abstract

pub_date

2012-03-01 00:00:00

pages

2475-82

issue

6

eissn

0175-7598

issn

1432-0614

journal_volume

93

pub_type

杂志文章

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