A new photobioreactor for continuous marennin production with a marine diatom: influence of the light intensity and the immobilised-cell matrix (alginate beads or agar layer).

abstract：:There are alarming reports of growing microbial resistance to all classes of antimicrobial agents used against different infections. Also the existing classes of anticancer drugs used against different tumours warrant the urgent search for more effective alternative agents for treatment. Broad-spectrum bioactivities o...

journal_title：Applied microbiology and biotechnology

authors： Rai M,Kon K,Ingle A,Duran N,Galdiero S,Galdiero M

更新日期：2014-03-01 00:00:00

abstract：:The extracellular production of Escherichia coli phytase was studied in fed-batch fermentations. Two different feeding strategies were compared: control by keeping the glucose concentration constant, and control by keeping a low constant oxygen level in the medium. For the feeding control based on glucose concentratio...

journal_title：Applied microbiology and biotechnology

authors： Kleist S,Miksch G,Hitzmann B,Arndt M,Friehs K,Flaschel E

更新日期：2003-06-01 00:00:00

abstract：:Acetic acid bacteria are well-known for their membrane-bound dehydrogenases rapidly oxidizing a variety of substrates in the periplasm. Since many acetic acid bacteria have not been successfully cultured in the laboratory yet, studying membrane-bound dehydrogenases directly from a metagenome of vinegar microbiota seem...

journal_title：Applied microbiology and biotechnology

authors： Peters B,Mientus M,Kostner D,Daniel R,Liebl W,Ehrenreich A

更新日期：2017-11-01 00:00:00

abstract：:The behavior of Streptomyces peucetius var. caesius N47 was studied in a glucose limited chemostat with a complex cultivation medium. The steady-state study yielded the characteristic constants mu (max) over 0.10 h(-1), Y (XS) 0.536 g g(-1), and m(S) 0.54 mg g(-1) h(-1). The product of secondary metabolism, epsilon-rh...

journal_title：Applied microbiology and biotechnology

authors： Kiviharju K,Moilanen U,Leisola M,Eerikäinen T

更新日期：2007-01-01 00:00:00

abstract：:Streptococcus mutans is the main cause of dental caries. We evaluated the therapeutic effect of variable regions of a llama heavy chain antibody fragments directed against S. mutans named S36-VHH (S for Streptococcus) alone or fused with glucose oxidase (GOx) from Aspergillus niger. Western blot analysis and ELISA rev...

journal_title：Applied microbiology and biotechnology

authors： Krüger C,Hultberg A,Marcotte H,Hermans P,Bezemer S,Frenken LG,Hammarström L

更新日期：2006-10-01 00:00:00

abstract：:A cephalosporin deacetylating acetyl xylan esterase was cloned from the genomic DNA of Bacillus subtilis CICC 20034 and functionally expressed in Escherichia coli. Its gene contained an open reading frame of 957 bp encoding 318 amino acids with a calculated mass of 35,607 Da, and it displayed significant identity to a...

journal_title：Applied microbiology and biotechnology

authors： Tian Q,Song P,Jiang L,Li S,Huang H

更新日期：2014-03-01 00:00:00

abstract：:The extrusion technology of blends formed by compounds with different physicochemical properties often results in new materials that present properties distinctive from its original individual constituents. Here, we report the use of melt extrusion of blends made from low-cost materials to produce a biodegradable foam...

journal_title：Applied microbiology and biotechnology

authors： Marcelino PR,Milani KM,Mali S,Santos OJ,de Oliveira AL

更新日期：2016-08-01 00:00:00

abstract：:Guanosine 5'-triphosphate (GTP) is the key substrate for biosynthesis of guanosine 5'-diphosphate (GDP)-L-fucose. In this study, improvement of GDP-L-fucose production was attempted by manipulating the biosynthetic pathway for guanosine nucleotides in recombinant Escherichia coli-producing GDP-L-fucose. The effects of...

journal_title：Applied microbiology and biotechnology

authors： Lee WH,Shin SY,Kim MD,Han NS,Seo JH

更新日期：2012-03-01 00:00:00

abstract：:In an effort to produce ricinoleic acid (RA), an important natural raw material with great values as a petrochemical replacement, in Schizosaccharomyces pombe, we introduced Claviceps purpurea oleate Δ12-hydroxylase gene (CpFAH12) to S. pombe, putting it under the control of an inducible nmt1 promoter. However, RA was...

journal_title：Applied microbiology and biotechnology

authors： Yazawa H,Holic R,Kumagai H,Uemura H

更新日期：2013-09-01 00:00:00

abstract：:Bacterial glucans have aroused increasing interest in commercial applications in the food and pharmaceutical sectors. A number of bacterial glucans have been reported over recent decades, and their structure, production, and functional properties have been extensively studied. In this paper, we review recent researche...

journal_title：Applied microbiology and biotechnology

authors： Xu L,Zhang J

更新日期：2016-11-01 00:00:00

abstract：:More than 70,000 different terpenoid structures are known so far; many of them offer highly interesting applications as pharmaceuticals, flavors and fragrances, or biofuels. Extraction of these compounds from their natural sources or chemical synthesis is-in many cases-technically challenging with low or moderate yiel...

journal_title：Applied microbiology and biotechnology

authors： Moser S,Pichler H

更新日期：2019-07-01 00:00:00

abstract：:In this study, we purified and molecularly characterized a cold-active beta-galactosidase from Arthrobacter psychrolactophilus strain F2. The purified beta-galactosidase from strain F2 exhibited high activity at 0 degrees C, and its optimum temperature and pH were 10 degrees C and 8.0, respectively. It was possible to...

journal_title：Applied microbiology and biotechnology

authors： Nakagawa T,Fujimoto Y,Ikehata R,Miyaji T,Tomizuka N

更新日期：2006-10-01 00:00:00

abstract：:Gene therapy, i.e., the expression in cells of genetic material with therapeutic activity, holds great promise for the treatment of human diseases. A delivery vehicle (vector), of either viral or non-viral origin, must be used to carry the foreign gene into a cell. Viral vectors take advantage of the facile integratio...

journal_title：Applied microbiology and biotechnology

authors： Thomas M,Klibanov AM

更新日期：2003-07-01 00:00:00

abstract：:A new pmoA gene-based PCR primer set was designed for detection of nitrite-dependent anaerobic oxidation of methane (n-damo) bacteria from four different ecosystems, namely rice paddy soil, freshwater reservoir, reed bed, and sludge from wastewater treatment plant. This primer set showed high specificity and efficienc...

journal_title：Applied microbiology and biotechnology

authors： Han P,Gu JD

更新日期：2013-12-01 00:00:00

abstract：:Previous investigations have reported that bacterial suspension cultures grow to higher stationary concentrations in space flight than on Earth; however, none of these investigations included extensive ground controls under varied inertial conditions. This study includes extensive controls and cell-growth data taken a...

journal_title：Applied microbiology and biotechnology

authors： Kacena MA,Merrell GA,Manfredi B,Smith EE,Klaus DM,Todd P

更新日期：1999-02-01 00:00:00

abstract：:With a cell concentration of 125 g dry biomass l-1 and a dilution rate of 0.1 h-1, Propionibacterium acidipropionici produces 30 g propionic acid l-1 from sugar with a productivity of 3 g l-1 h-1. The yield of propionic acid is approx. 0.36-0.45 g propionic acid g-1 sucrose and is independent of the dilution rate and ...

journal_title：Applied microbiology and biotechnology

authors： Quesada-Chanto A,Afschar AS,Wagner F

更新日期：1994-06-01 00:00:00

abstract：:Some strains belonging to the genera Citrobacter and Enterobacter have been reported to produce chitin/chitosan-like bioflocculants (BFs) from acetate. In this study, to investigate the distribution of the BF-producing potential in the genus Citrobacter and to screen stably and highly BF-producing strains, we obtained...

journal_title：Applied microbiology and biotechnology

authors： Kimura K,Inoue T,Kato D,Negoro S,Ike M,Takeo M

更新日期：2013-11-01 00:00:00

abstract：:Phytoplankton blooms are predictable features of marine and freshwater habitats. Despite a good knowledge base of the environmental factors controlling blooms, complex interactions between the bacterial and archaeal communities and phytoplankton bloom taxa are only now emerging. Here, the current research on bacterial...

journal_title：Applied microbiology and biotechnology

authors： Song L

更新日期：2017-06-01 00:00:00

abstract：:Biocatalytic asymmetric preparation of (R)-1,3-butanediol has been attracting much attention in pharmaceuticals industry. A new ideal strain, ZJB-09162, which exhibited high reduction activity and excellent (R)-stereospecificity towards 4-hydroxy-2-butanone, has been successfully isolated from soil samples. Based on m...

journal_title：Applied microbiology and biotechnology

authors： Zheng RC,Ge Z,Qiu ZK,Wang YS,Zheng YG

更新日期：2012-05-01 00:00:00

abstract：:The engineered Saccharomyces cerevisiae strain △faa1△faa4 [Acot5s] was demonstrated to accumulate more free fatty acids (FFA) previously. Here, comparative proteomic analysis was performed to get a global overview of metabolic regulation in the strain. Over 500 proteins were identified, and 82 of those proteins were f...

journal_title：Applied microbiology and biotechnology

authors： Chen L,Lee JJL,Zhang J,Chen WN

更新日期：2016-02-01 00:00:00

abstract：:This study aimed to model the inactivation of Lactobacillus brevis DSM 6235 while retaining the viability of yeasts during washing brewer's yeast with phosphoric acid and chlorine dioxide. The independent variables in the acid washing were pH (1-3) and temperature (1-9 °C), whereas in the washing with chlorine dioxide...

journal_title：Applied microbiology and biotechnology

authors： Munford ARG,Chaves RD,Granato D,Sant'Ana AS

更新日期：2020-05-01 00:00:00

abstract：:5-Hydroxytryptophan (5-HTP) is the precursor of the neurotransmitter serotonin and has been used for the treatment of various diseases such as depression, insomnia, chronic headaches, and binge eating associated obesity. The production of 5-HTP had been achieved in our previous report, by the development of a recombin...

journal_title：Applied microbiology and biotechnology

authors： Xu D,Fang M,Wang H,Huang L,Xu Q,Xu Z

更新日期：2020-03-01 00:00:00

abstract：:The original version of this article was revised: After publication of this article, the publisher was notified that Michael Klocke has been listed as an author without his consent. ...

journal_title：Applied microbiology and biotechnology

authors： Carabeo-Pérez A,Guerra-Rivera G,Ramos-Leal M,Jiménez-Hernández J

更新日期：2019-12-01 00:00:00

abstract：:In this study, we applied a 16S ribosomal RNA (rRNA) metagenomics approach to survey inanimate hospital environments (IHEs) in a respiratory care center (RCC). A total of 16 samples, including 9 from medical devices and 7 from workstations, were analyzed. Besides, clinical isolates were retrospectively analyzed during...

journal_title：Applied microbiology and biotechnology

authors： Tang CY,Yiu SM,Kuo HY,Tan TS,Liao KH,Liu CC,Hon WK,Liou ML

更新日期：2015-03-01 00:00:00

abstract：:Herein, we present data demonstrating that the application of initial cell culture enrichment could significantly improve mycoplasma testing methods based on the nucleic acid amplification technology (NAT) including a polymerase chain reaction (PCR)/microarray method. The results of the study using Vero cells demonstr...

journal_title：Applied microbiology and biotechnology

authors： Kong H,Volokhov DV,George J,Ikonomi P,Chandler D,Anderson C,Chizhikov V

更新日期：2007-11-01 00:00:00

abstract：:Steroid phosphate esters are very rare natural lipids that have been comparatively recently isolated from fractions of polar lipids of marine sponges and starfish. These steroids exhibit interesting biological activities. When using the PASS computer program, we showed that many of steroid phosphate esters showed anti...

journal_title：Applied microbiology and biotechnology

authors： Dembitsky VM,Gloriozova TA,Savidov N

更新日期：2018-09-01 00:00:00

abstract：:The effects of copper (Cu), chromium (Cr), cadmium (Cd), lead (Pb) and zinc (Zn) on the biotransformation of organic acids (acetate, propionate and butyrate) and H2 were assessed in serum-bottle microcosms. Experiments were performed over a range of metal concentrations (20-200 mg/l) using biomass from an anaerobic bi...

journal_title：Applied microbiology and biotechnology

authors： Kong IC,Hubbard JS,Jones WJ

更新日期：1994-11-01 00:00:00

abstract：:Diastereoselective reduction of diketones with Lactobacillus kefir DSM 20587 was examined. The reduction of both oxo-functions proceeded highly diastereoselectively. (2 R,5 R)-Hexanediol 3 was produced starting from (2,5)-hexanedione 1 in quantitative yields with enantiomeric excess >99% and diastereomeric excess >99%...

journal_title：Applied microbiology and biotechnology

authors： Haberland J,Kriegesmann A,Wolfram E,Hummel W,Liese A

更新日期：2002-04-01 00:00:00

abstract：:The merC gene from the Tn21-encoded mer operon has potential uses as a molecular tool for bioremediation. It was overexpressed as the fusion proteins MerC-Sso1p or MerC-Vam3p in Saccharomyces cerevisiae. Green fluorescent protein (GFP)-MerC-Sso1p fusion proteins located primarily in the plasma membrane, although some ...

journal_title：Applied microbiology and biotechnology

authors： Kiyono M,Miyahara K,Sone Y,Pan-Hou H,Uraguchi S,Nakamura R,Sakabe K

更新日期：2010-03-01 00:00:00

abstract：:Staphylococcus aureus-induced bovine mastitis causes significant losses to the dairy industry and available vaccines do not confer adequate protection. As a more attractive alternative, we propose the use of antibody (Ab) therapy. In our previous study, we constructed a bovine single-chain variable fragment (scFv) Ab ...

journal_title：Applied microbiology and biotechnology

authors： Wang M,Wang T,Guan Y,Wang F,Zhu J

更新日期：2019-02-01 00:00:00