Isolation and characterization of an antifungal peptide with antiproliferative activity from seeds of Phaseolus vulgaris cv. 'Spotted Bean'.

Abstract:

:A 7.3-kDa antifungal peptide with an N-terminal sequence exhibiting remarkable homology to defensins from other leguminous plants was isolated from Phaseolus vulgaris cv. 'Spotted Bean'. The isolation procedure involved ion exchange chromatography on O-diethylaminoethyl (DEAE) cellulose, affinity chromatography on Affi-gel blue gel, ion exchange chromatography on SP-Sepharose, and gel filtration by fast protein liquid chromatography on Superdex 75. The peptide was unadsorbed on DEAE-cellulose and adsorbed on Affi-gel blue gel and SP-Sepharose. It exerted an antifungal action on Fusarium oxysporum and Mycosphaerella arachidicola. It inhibited mycelial growth in F. oxysporum with an IC(50) value of 1.8 microM. It suppressed [methyl-(3)H]-thymidine incorporation by leukemia L1210 cells and MBL2 cells with an IC(50) value of 4.0 and 9.0 microM, respectively. There was no effect on HIV-1 reverse transcriptase activity when the peptide was tested up to 0.1 mM.

authors

Wang HX,Ng TB

doi

10.1007/s00253-006-0650-9

subject

Has Abstract

pub_date

2007-02-01 00:00:00

pages

125-30

issue

1

eissn

0175-7598

issn

1432-0614

journal_volume

74

pub_type

杂志文章
  • Biodegradation of fat, oil and grease (FOG) deposits under various redox conditions relevant to sewer environment.

    abstract::Fat, oil and, grease (FOG) deposits are one primary cause of sanitary sewer overflows (SSOs). While numerous studies have examined the formation of FOG deposits in sewer pipes, little is known about their biodegradation under sewer environments. In this study, FOG deposit biodegradation potential was determined by stu...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-015-6457-9

    authors: He X,Zhang Q,Cooney MJ,Yan T

    更新日期:2015-07-01 00:00:00

  • Bioproducts from Aureobasidium pullulans, a biotechnologically important yeast.

    abstract::It has been well documented that Aureobasidium pullulans is widely distributed in different environments. Different strains of A. pullulans can produce amylase, proteinase, lipase, cellulase, xylanase, mannanase, transferases, pullulan, siderophore, and single-cell protein, and the genes encoding proteinase, lipase, c...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-009-1882-2

    authors: Chi Z,Wang F,Chi Z,Yue L,Liu G,Zhang T

    更新日期:2009-04-01 00:00:00

  • Protein compositional analysis of inclusion bodies produced in recombinant Escherichia coli.

    abstract::Culture conditions favouring the simultaneous formation of soluble protein and inclusion bodies (IBs) were chosen for producing the cytoplasmic protein beta-galactosidase or the periplasmic protein TEM-beta-lactamase. Soluble and insoluble cell fractions of Escherichia coli producing either beta-galactosidase or TEM-b...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00240735

    authors: Rinas U,Bailey JE

    更新日期:1992-08-01 00:00:00

  • Characterization and complementation of a cephalosporin-deficient mutant of Streptomyces clavuligerus NRRL 3585.

    abstract::We have characterized a mutant of Streptomyces clavuligerus NRRL 3585 which is almost completely blocked in cephalosporin biosynthesis and exhibits depressed activities of both the delta(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV) synthetase and cyclase enzymes of the cephalosporin pathway. A wild-type DNA region ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00173728

    authors: Piret J,Resendiz B,Mahro B,Zhang JY,Serpe E,Romero J,Connors N,Demain AL

    更新日期:1990-02-01 00:00:00

  • Reconstructing the clostridial n-butanol metabolic pathway in Lactobacillus brevis.

    abstract::A Lactobacillus brevis strain with the ability to synthesize butanol from glucose was constructed by metabolic engineering. The genes crt, bcd, etfB, etfA, and hbd, composing the bcs-operon, and the thl gene encode the enzymes of the lower part of the clostridial butanol pathway (crotonase, butyryl-CoA-dehydrogenase, ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-010-2480-z

    authors: Berezina OV,Zakharova NV,Brandt A,Yarotsky SV,Schwarz WH,Zverlov VV

    更新日期:2010-06-01 00:00:00

  • Coupling effects of osmotic pressure and temperature on the viability of Saccharomyces cerevisiae.

    abstract::The osmotic tolerance of cells of Saccharomyces cerevisiae as a function of glycerol concentration and temperature has been investigated. Results show that under isothermal conditions (25 degrees C) cells are resistant (94% viability) to hyperosmotic treatment at 49.2 MPa. A thigher osmotic pressure, cell viability de...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s002530100619

    authors: Beney L,Marechal PA,Gervais P

    更新日期:2001-08-01 00:00:00

  • High-level expression of aryl-alcohol oxidase 2 from Pleurotus eryngii in Pichia pastoris for production of fragrances and bioactive precursors.

    abstract::The fungal secretome comprises various oxidative enzymes participating in the degradation of lignocellulosic biomass as a central step in carbon recycling. Among the secreted enzymes, aryl-alcohol oxidases (AAOs) are of interest for biotechnological applications including production of bio-based precursors for plastic...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-020-10878-4

    authors: Jankowski N,Koschorreck K,Urlacher VB

    更新日期:2020-11-01 00:00:00

  • Induction, purification and characterization of alpha-N-acetylgalactosaminidase from Aspergillus Niger.

    abstract::A set of filamentous fungi (42 strains) was screened for alpha-N-acetylgalactosaminidase activity, and a series of inducers and different cultivation conditions were tested. Enzyme production by the best producer Aspergillus niger CCIM K2 was optimized and scaled up. alpha-N-Acetylgalactosaminidase was purified to app...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-008-1485-3

    authors: Weignerová L,Filipi T,Manglová D,Kren V

    更新日期:2008-07-01 00:00:00

  • Metabolites from nematophagous fungi and nematicidal natural products from fungi as an alternative for biological control. Part I: metabolites from nematophagous ascomycetes.

    abstract::Plant-parasitic nematodes are estimated to cause global annual losses of more than US$ 100 billion. The number of registered nematicides has declined substantially over the last 25 years due to concerns about their non-specific mechanisms of action and hence their potential toxicity and likelihood to cause environment...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-015-7233-6

    authors: Degenkolb T,Vilcinskas A

    更新日期:2016-05-01 00:00:00

  • Bringing nitrilase sequences from databases to life: the search for novel substrate specificities with a focus on dinitriles.

    abstract::The aim of this study was to discover new nitrilases with useful activities, especially towards dinitriles that are precursors of high-value cyano acids. Genes coding for putative nitrilases of different origins (fungal, plant, or bacterial) with moderate similarities to known nitrilases were selected by mining the Ge...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-015-7023-1

    authors: Veselá AB,Rucká L,Kaplan O,Pelantová H,Nešvera J,Pátek M,Martínková L

    更新日期:2016-03-01 00:00:00

  • Advances in acrylamide bioproduction catalyzed with Rhodococcus cells harboring nitrile hydratase.

    abstract::Acrylamide is an important bulk chemical used for producing polyacrylamide, which is widely applied in diverse fields, such as enhanced oil recovery and water treatment. Acrylamide production with a superior biocatalyst, free-resting Rhodococcus cells containing nitrile hydratase (NHase), has been proven to be simple ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-019-10284-5

    authors: Jiao S,Li F,Yu H,Shen Z

    更新日期:2020-02-01 00:00:00

  • Effect of Lactobacillus plantarum expressing multifunctional glycoside hydrolases on the characteristics of alfalfa silage.

    abstract::For the first time, Lactobacillus plantarum strains carrying heterologous genes encoding multifunctional glycoside hydrolases were constructed and used as additives for alfalfa silage. The chemical characteristics, nonstructural carbohydrate composition, and fermentation quality of alfalfa silage were examined. The su...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-019-10097-6

    authors: Guo J,Xie Y,Yu Z,Meng G,Wu Z

    更新日期:2019-10-01 00:00:00

  • Improved beta-thujaplicin production in Cupressus lusitanica suspension cultures by fungal elicitor and methyl jasmonate.

    abstract::Production of a novel antimicrobial tropolone, beta-thujaplicin, in Cupressus lusitanica suspension cultures was studied by using a variety of chemicals and fungal elicitors. Sodium alginate, chitin, and methyl jasmonate resulted in 2-, 2.5-, and 3-fold higher beta-thujaplicin production, respectively, than in the con...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s002530000555

    authors: Zhao J,Fujita K,Yamada J,Sakai K

    更新日期:2001-04-01 00:00:00

  • Isolation and lipid degradation profile of Raoultella planticola strain 232-2 capable of efficiently catabolizing edible oils under acidic conditions.

    abstract::The lipids (fats and oils) degradation capabilities of soil microorganisms were investigated for possible application in treatment of lipids-contaminated wastewater. We isolated a strain of the bacterium Raoultella planticola strain 232-2 that is capable of efficiently catabolizing lipids under acidic conditions such ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-012-3982-7

    authors: Sugimori D,Watanabe M,Utsue T

    更新日期:2013-01-01 00:00:00

  • Process characterization and influence of alternative carbon sources and carbon-to-nitrogen ratio on organic acid production by Aspergillus oryzae DSM1863.

    abstract::L-Malic acid and fumaric acid are C4 dicarboxylic organic acids and considered as promising chemical building blocks. They can be applied as food preservatives and acidulants in rust removal and as polymerization starter units. Molds of the genus Aspergillus are able to produce malic acid in large quantities from gluc...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-014-5614-x

    authors: Ochsenreither K,Fischer C,Neumann A,Syldatk C

    更新日期:2014-06-01 00:00:00

  • The plant strengthening root endophyte Piriformospora indica: potential application and the biology behind.

    abstract::The successful conversion of plant production systems from conventional resource-exhausting to sustainable strategies depends on knowledge-based management of environmental factors. Root-inhabiting fungi came more and more into focus because their hyphae connect in ideal manner resources and challenges of the surround...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-012-4506-1

    authors: Franken P

    更新日期:2012-12-01 00:00:00

  • Genome engineering in actinomycetes using site-specific recombinases.

    abstract::The rational modification of the actinomycetes genomes has a variety of applications in research, medicine, and biotechnology. The use of site-specific recombinases allows generation of multiple mutations, large DNA deletions, integrations, and inversions and may lead to significant progress in all of these fields. De...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-013-4866-1

    authors: Myronovskyi M,Luzhetskyy A

    更新日期:2013-06-01 00:00:00

  • Optimization of erythritol and glycerol accumulation in conidia of Beauveria bassiana by solid-state fermentation, using response surface methodology.

    abstract::Entomopathogenic fungi are widely produced for use as mycoinsecticides. Therefore, improvement of the shelf life of fungal propagules under good and adverse conditions should be a pre-requisite of their production. In order to improve conidial physiology as well as mycoinsecticide efficiency, culture conditions may be...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-005-1901-x

    authors: Tarocco F,Lecuona RE,Couto AS,Arcas JA

    更新日期:2005-09-01 00:00:00

  • Influence of the surface speciation on biofilm attachment to chalcopyrite by Acidithiobacillus thiooxidans.

    abstract::Surfaces of massive chalcopyrite (CuFeS2) electrodes were modified by applying variable oxidation potential pulses under growth media in order to induce the formation of different secondary phases (e.g., copper-rich polysulfides, S n(2-); elemental sulfur, S(0); and covellite, CuS). The evolution of reactivity (oxidat...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-012-4099-8

    authors: Lara RH,García-Meza JV,González I,Cruz R

    更新日期:2013-03-01 00:00:00

  • Efficient multi-enzyme-catalyzed CDP-choline production driven by an ATP donor module.

    abstract::Cytidine diphosphate choline (CDP-choline) has been applied for treating acute craniocerebral injury and allowing recovery of consciousness after brain surgery. In this study, an acetate kinase (ACK)/acetyl phosphate system was used to supply ATP and combined with Escherichia coli-overexpressed CMP kinase (CMK), NDP k...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-016-7874-0

    authors: Liu Y,Wang J,Xu C,Chen Y,Yang J,Liu D,Niu H,Jiang Y,Yang S,Ying H

    更新日期:2017-02-01 00:00:00

  • Biotransformation of gallic acid by Beauveria sulfurescens ATCC 7159.

    abstract::Preparative-scale fermentation of gallic acid (3,4,5-trihydroxybenzoic acid) (1) with Beauveria sulfurescens ATCC 7159 gave two new glucosidated compounds, 4-(3,4-dihydroxy-6-hydroxymethyl-5-methoxy-tetrahydro-pyran-2-yloxy)-3-hydroxy-5-methoxy-benzoic acid (4), 3-hydroxy-4,5-dimethoxy-benzoic acid 3,4-dihydroxy-6-hyd...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-006-0692-z

    authors: Hsu FL,Yang LM,Chang SF,Wang LH,Hsu CY,Liu PC,Lin SJ

    更新日期:2007-03-01 00:00:00

  • Metabolic reconstruction of Pseudomonas chlororaphis ATCC 9446 to understand its metabolic potential as a phenazine-1-carboxamide-producing strain.

    abstract::Pseudomonas chlororaphis is a plant-associated bacterium with reported antagonistic activity against different organisms and plant growth-promoting properties. P. chlororaphis possesses exciting biotechnological features shared with another Pseudomonas with a nonpathogenic phenotype. Part of the antagonistic role of P...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-020-10913-4

    authors: Moreno-Avitia F,Utrilla J,Bolívar F,Nogales J,Escalante A

    更新日期:2020-12-01 00:00:00

  • Structural modulation of gut microbiota in Bama minipigs in response to treatment with a "growth-promoting agent", salbutamol.

    abstract::Even though salbutamol (SAL) had remarkable effects on the enhancement of growth rate and carcass composition in different livestock species such as cattle, pigs, sheep and poultry, it was banned as a growth promoter because of its adverse effects on health. However, the specific mechanism by which salbutamol enhances...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-017-8329-y

    authors: Lu C,Zhou J,Li Y,Zhang D,Wang Z,Li Y,Cheong L,Zhang C,Su X

    更新日期:2017-07-01 00:00:00

  • Motility influences biofilm architecture in Escherichia coli.

    abstract::Eight Escherichia coli strains were studied in minimal medium with a continuous flow system using confocal microscopy. K12 wild-type strains ATCC 25404 and MG1655 formed the best biofilms ( approximately 43 microm thick, 21 to 34% surface coverage). JM109, DH5alpha, and MG1655 motA formed intermediate biofilms ( appro...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-005-0263-8

    authors: Wood TK,González Barrios AF,Herzberg M,Lee J

    更新日期:2006-09-01 00:00:00

  • Label-free Raman imaging of live osteosarcoma cells with multivariate analysis.

    abstract::Confocal Raman microspectral imaging (CRMI) is an advanced cell-imaging method that maps endogenous molecular compositions with their unique spectral fingerprint indicators. The aim of this work was to provide a visualized understanding of subcellular features of live osteosarcoma cells using a 532-nm laser excitation...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-019-09952-3

    authors: Li J,Qin J,Zhang X,Wang R,Liang Z,He Q,Wang Z,Wang K,Wang S

    更新日期:2019-08-01 00:00:00

  • Synthesis of gamma-aminobutyric acid (GABA) by Lactobacillus plantarum DSM19463: functional grape must beverage and dermatological applications.

    abstract::Agriculture surplus were used as substrates to synthesize gamma-aminobutyric acid (GABA) by Lactobacillus plantarum DSM19463 for the manufacture of a functional beverage or as a novel application for dermatological purposes. Dilution of the grape must to 1 or 4% (w/v) of total carbohydrates favored higher cell yield a...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-009-2370-4

    authors: Di Cagno R,Mazzacane F,Rizzello CG,De Angelis M,Giuliani G,Meloni M,De Servi B,Gobbetti M

    更新日期:2010-03-01 00:00:00

  • Phosphate starvation controls lactose metabolism to produce recombinant protein in Escherichia coli.

    abstract::Phosphate is one of the major constituents in growth media. It closely regulates central carbon and energy metabolism. Biochemical reactions in central carbon metabolism are known to be regulated by phosphorylation and dephosphorylation of enzymes. Phosphate scarcity can limit microbial productivity. However, microorg...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-020-10935-y

    authors: Pandi K,Chauhan AS,Khan WH,Rathore AS

    更新日期:2020-11-01 00:00:00

  • Laccase from the medicinal mushroom Agaricus blazei: production, purification and characterization.

    abstract::The medicinal mushroom Agaricus blazei produced high amounts of laccase (up to 5,000 units l(-1)) in a complex, agitated liquid medium based on tomato juice, while only traces of the enzyme (<100 units l(-1)) were detected in synthetic glucose-based medium. Purification of the enzyme required three chromatographic ste...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-004-1861-6

    authors: Ullrich R,Huong le M,Dung NL,Hofrichter M

    更新日期:2005-05-01 00:00:00

  • Metagenome, metatranscriptome, and metaproteome approaches unraveled compositions and functional relationships of microbial communities residing in biogas plants.

    abstract::The production of biogas by anaerobic digestion (AD) of agricultural residues, organic wastes, animal excrements, municipal sludge, and energy crops has a firm place in sustainable energy production and bio-economy strategies. Focusing on the microbial community involved in biomass conversion offers the opportunity to...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-018-8976-7

    authors: Hassa J,Maus I,Off S,Pühler A,Scherer P,Klocke M,Schlüter A

    更新日期:2018-06-01 00:00:00

  • Initial stages of microbiologically influenced tarnishing on titanium after 20 months of immersion in freshwater.

    abstract::This paper studies the initial stages of iridescent tarnishes on titanium heat exchanger tubes in contact with running freshwater on the river Tagus in Spain for up to 20 months. Electrochemical impedance spectroscopy (EIS), scanning electron microscopy [(SEM with energy dispersive X-ray (EDX)] and X-ray photoelectron...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-003-1472-7

    authors: Moreno DA,Cano E,Ibars JR,Polo JL,Montero F,Bastidas JM

    更新日期:2004-05-01 00:00:00