Bacteriophage-encoded virion-associated enzymes to overcome the carbohydrate barriers during the infection process.

abstract：:To measure the toxicity experienced by superoxide-generating compounds, two plasmids were constructed in which the superoxide-inducible fpr and zwf promoters from Escherichia coli were fused to promoterless Vibrio fischeri luxCDABE operon present in plasmid pUCD615. The bioluminescent response of E. coli harboring the...

journal_title：Applied microbiology and biotechnology

authors： Niazi JH,Kim BC,Gu MB

更新日期：2007-04-01 00:00:00

abstract：:Escherichia coli KJ122 was previously engineered to produce high concentration and yield of succinate in mineral salt medium containing glucose and sucrose under anaerobic conditions. However, this strain does not efficiently utilize xylose. To improve the xylose uptake and utilization in the strain KJ122, xylFGH and ...

journal_title：Applied microbiology and biotechnology

authors： Khunnonkwao P,Jantama SS,Kanchanatawee S,Jantama K

更新日期：2018-01-01 00:00:00

abstract：:Nitrilase-mediated hydrolysis of isobutylsuccinonitrile (IBSN) is a highly attractive approach for (S)-3-cyano-5-methylhexanoic acid ((S)-CMHA), the critical chiral intermediate of pregabalin. In this study, a robust nitrilase from Arabis alpina (AaNIT) was screened and engineered. The N258D mutant was obtained with h...

journal_title：Applied microbiology and biotechnology

authors： Zhang Q,Wu ZM,Hao CL,Tang XL,Zheng RC,Zheng YG

更新日期：2019-07-01 00:00:00

abstract：:Microalgal starch is a potential feedstock for biofuel production. Nutrient stress is widely used to stimulate starch accumulation in microalgae. Cell growth and starch accumulation in the marine green microalga Tetraselmis subcordiformis were evaluated under extracellular phosphorus deprivation with initial cell dens...

journal_title：Applied microbiology and biotechnology

authors： Yao CH,Ai JN,Cao XP,Xue S

更新日期：2013-07-01 00:00:00

abstract：:The carbon and nitrogen sources most suitable for L-asparaginase production by Enterobacter aerogenes were selected and their concentrations optimized in shake-flask cultures. Sodium citrate (1.0%) and diammonium hydrogen phosphate (0.16%) proved to be the best sources of carbon and nitrogen, respectively. Nitrogen ca...

journal_title：Applied microbiology and biotechnology

authors： Mukherjee J,Majumdar S,Scheper T

更新日期：2000-02-01 00:00:00

abstract：:Previous investigations have reported that bacterial suspension cultures grow to higher stationary concentrations in space flight than on Earth; however, none of these investigations included extensive ground controls under varied inertial conditions. This study includes extensive controls and cell-growth data taken a...

journal_title：Applied microbiology and biotechnology

authors： Kacena MA,Merrell GA,Manfredi B,Smith EE,Klaus DM,Todd P

更新日期：1999-02-01 00:00:00

abstract：:The sustainable and practical degradation of intact chicken feathers by a newly isolated thermophilic bacterium Meiothermus ruber H328 was presented with extensive data. Aerobic cultivation with moderately thermophilic strain H328 at 55 degrees C for 6 days led to the apparently complete decay of the truly intact feat...

journal_title：Applied microbiology and biotechnology

authors： Matsui T,Yamada Y,Mitsuya H,Shigeri Y,Yoshida Y,Saito Y,Matsui H,Watanabe K

更新日期：2009-04-01 00:00:00

abstract：:D-Tagatose is a hexoketose monosaccharide sweetener, which is an isomer of D-galactose and is rarely found in nature. Recently, there has been industrial interest in D-tagatose as a low-calorie sugar-substituting sweetener. This article describes the properties and metabolism of tagatose as well as its commercial impo...

journal_title：Applied microbiology and biotechnology

authors： Kim P

更新日期：2004-08-01 00:00:00

abstract：:Manipulation of product heterogeneity was attempted by using yeast extract as nitrogen source in Alternaria alternata S-f6 transformation process of 4'-demethylepipodophyllotoxin. When the nitrogen source of NaNO(3) was replaced by yeast extract, the heterogeneity of biotransformation products was significantly varied...

journal_title：Applied microbiology and biotechnology

authors： Zhao W,Li HM,Wan DJ,Tang YJ

更新日期：2012-01-01 00:00:00

abstract：:A newly isolated strain of Cunninghamella echinulata grown on glucose produced significant quantities of biomass and cellular lipids in media with high C/N ratio. The oil yield from glucose consumed increased after nitrogen exhaustion in the growth medium, but gamma-linolenic acid (GLA) content in cellular oil systema...

journal_title：Applied microbiology and biotechnology

authors： Gema H,Kavadia A,Dimou D,Tsagou V,Komaitis M,Aggelis G

更新日期：2002-03-01 00:00:00

abstract：:The 454 pyrosequencing technique was applied to evaluate microbial community composition in sediment and water samples collected from the river receiving effluents from a swine farm and a farmhouse restaurant, respectively. For each sample, 4,600 effective sequences were selected and used to do the bacterial diversity...

journal_title：Applied microbiology and biotechnology

authors： Lu XM,Lu PZ

更新日期：2014-09-01 00:00:00

abstract：:Epitope vaccine based on the enzyme urease of Helicobacter pylori is a promising option for prophylactic and therapeutic vaccination against H. pylori infection. In our previous study, the epitope vaccine CTB-UA, which was composed of the mucosal adjuvant cholera toxin B subunit (CTB) and an epitope (UreA₁₈₃₋₂₀₃) from...

journal_title：Applied microbiology and biotechnology

authors： Guo L,Liu K,Xu G,Li X,Tu J,Tang F,Xing Y,Xi T

更新日期：2012-09-01 00:00:00

abstract：:Intervention strategies for obesity are global issues that require immediate attention. The objective of this study was to assess the possibility that Clostridium butyricum and its potential components could reduce lipogenesis. Co-culture experiments of Caco-2 cells and 1 × 10(6), 1 × 10(7), and 1 × 10(8) CFU/ml of C....

journal_title：Applied microbiology and biotechnology

authors： Zhao X,Guo Y,Liu H,Gao J,Nie W

更新日期：2014-09-01 00:00:00

abstract：:MeBglD2, a β-glycosidase that is highly activated in the presence of various monosaccharides and disaccharides, was isolated from a soil metagenomic library. MeBglD2 had not only β-glucosidase activity but also β-galactosidase and β-fucosidase activities. MeBglD2 β-glucosidase activity increased in a cellobiose concen...

journal_title：Applied microbiology and biotechnology

authors： Matsuzawa T,Yaoi K

更新日期：2017-01-01 00:00:00

abstract：:Arthrobacter sp. strain MIS38 was transformed with a shuttle vector containing the kanamycin resistant gene kan (derived from Tn5) by an electroporation method. This shuttle vector is from Brevibacterium lactofermentum and Escherichia coli, pULRS8. The following optimal condition of electroporation was determined. A s...

journal_title：Applied microbiology and biotechnology

authors： Morikawa M,Daido H,Pongpobpibool S,Imanaka T

更新日期：1994-11-01 00:00:00

abstract：:Thermochemical processing of biomass by fast pyrolysis provides a nonenzymatic route for depolymerization of biomass into sugars that can be used for the biological production of fuels and chemicals. Fermentative utilization of this bio-oil faces two formidable challenges. First is the fact that most bio-oil-associate...

journal_title：Applied microbiology and biotechnology

authors： Jarboe LR,Wen Z,Choi D,Brown RC

更新日期：2011-09-01 00:00:00

abstract：:In Catharanthus roseus cell cultures the time courses of four enzyme activities, tryptophan decarboxylase (TDC), strictosidine synthase (SSS), geraniol-10-hydroxylase (G10H) and anthranilate synthase (AS), and alkaloid accumulation were compared under two different culture conditions (low-inoculum density and high-ino...

journal_title：Applied microbiology and biotechnology

authors： Moreno PR,Schlatmann JE,van der Heijden R,van Gulik WM,ten Hoopen HJ,Verpoorte R,Heijnen JJ

更新日期：1993-04-01 00:00:00

abstract：:Nanobiotechnology raises fascinating possibilities for new analytical assays in various fields such as bioelectronic assembly, biomechanics and sampling techniques, as well as in chips or micromachined devices. Recently, nanotechnology has greatly impacted biotechnological research with its potential applications in s...

journal_title：Applied microbiology and biotechnology

authors： Lee SJ,Lee SY

更新日期：2004-04-01 00:00:00

abstract：:We report the successful high-yield expression of Candida utilis uricase in Escherichia coli and the establishment of an efficient three-step protein purification protocol. The purity of the recombinant protein, which was confirmed to be C. utilis uricase by sodium dodecyl sulfate-polyacrylamide gel electrophoresis an...

journal_title：Applied microbiology and biotechnology

authors： Liu X,Wen M,Li J,Zhai F,Ruan J,Zhang L,Li S

更新日期：2011-11-01 00:00:00

abstract：:Significant decreases in the apparent viscosity of a bacterial biofilm suspension were measured following addition of sodium, potassium, magnesium, or calcium salts, whereas iron salts increased the viscosity. Electrostatic interactions contribute to biofilm cohesion and iron cations are potent crosslinkers of the bio...

journal_title：Applied microbiology and biotechnology

authors： Chen X,Stewart PS

更新日期：2002-09-01 00:00:00

abstract：:Baicalin (baicalein 7-O-β-D-glucuronide) is one of the major flavonoid glucuronides found in traditional herbal medicines. Because its aglycone, baicalein, is absorbed more quickly and shows more effective properties than baicalin, the conversion of baicalin into baicalein by β-glucuronidase (GUS) has drawn the attent...

journal_title：Applied microbiology and biotechnology

authors： Sakurama H,Kishino S,Uchibori Y,Yonejima Y,Ashida H,Kita K,Takahashi S,Ogawa J

更新日期：2014-05-01 00:00:00

abstract：:The temporal and spatial distribution of the genus Bacillus and Clostridium histolyticum group in swine manure composting was determined by fluorescent in situ hybridization using fluorescently labeled 16S rRNA-targeted oligonucleotide probes LGC353b and Chis150, respectively. The temporal distribution of total bacter...

journal_title：Applied microbiology and biotechnology

authors： Yi J,Zheng R,Li F,Chao Z,Deng CY,Wu J

更新日期：2012-03-01 00:00:00

abstract：:Glycerol formation is vital for reoxidation of nicotinamide adenine dinucleotide (reduced form; NADH) under anaerobic conditions and for the hyperosmotic stress response in the yeast Saccharomyces cerevisiae. However, relatively few studies have been made on hyperosmotic stress under anaerobic conditions. To study the...

journal_title：Applied microbiology and biotechnology

authors： Modig T,Granath K,Adler L,Lidén G

更新日期：2007-05-01 00:00:00

abstract：:A comparative study was carried out in anaerobic batch cultures on 20 g/l of either glycerol or glucose using two propionibacteria strains, Propionibacterium acidipropionici and Propionibacterium freudenreichii ssp. shermanii. In all cases, fermentation end-products were the same and consisted of propionic acid as the...

journal_title：Applied microbiology and biotechnology

authors： Himmi EH,Bories A,Boussaid A,Hassani L

更新日期：2000-04-01 00:00:00

abstract：:2-Hydroxyalkanoates (2HAs) have become the new monomeric constituents of bacterial polyhydroxyalkanoates (PHAs). PHAs containing 2HA monomers, lactate (LA), glycolate (GL), and 2-hydroxybutyrate (2HB) can be synthesized by engineered microbes in which the broad substrate specificities of PHA synthase and propionyl-CoA...

journal_title：Applied microbiology and biotechnology

authors： Matsumoto K,Taguchi S

更新日期：2013-09-01 00:00:00

abstract：:The binaphthyl derivative cladosporol 3 was supplied from 60 to 200 mg l(-1) to shaken cultures of Cladosporium cf. cladosporioides. Compared to blank, fungal biomass was not affected by adding cladosporol till 100 mg l(-1): it rather increased at higher ratios between 150 and 200 mg l(-1). The production of the major...

journal_title：Applied microbiology and biotechnology

authors： Assante G,Bava A,Nasini G

更新日期：2006-02-01 00:00:00

abstract：:The effect of vitamins on the aerobic degradation and dechlorination of 2-chlorophenol and 4-chlorophenol by Pseudomonas pickettii, strain LD1, and 4-chlorobiphenyl by Pseudomonas sp. strain CPE1 was determined. These microorganisms are capable of using the target compounds as the sole carbon and energy source, but do...

journal_title：Applied microbiology and biotechnology

authors： Kafkewitz D,Fava F,Armenante PM

更新日期：1996-11-01 00:00:00

abstract：:Humus as an electron mediator is recognized as an effective strategy to improve the biological transformation and degradation of toxic substances, yet the action of humus in microbial detoxification of chromate is still unknown. In this study, a humus-reducing strain 3C(3) was isolated from mangrove sediment. Based on...

journal_title：Applied microbiology and biotechnology

authors： Hong Y,Wu P,Li W,Gu J,Duan S

更新日期：2012-03-01 00:00:00

abstract：:Application of soil amendments has been wildly used to increase soil pH and control bacterial wilt. However, little is known about causal shifts in the rhizosphere microbial community of crops, especially when the field naturally harbors the disease of bacterial wilt to tobacco for many years due to long-term continuo...

journal_title：Applied microbiology and biotechnology

authors： Shen G,Zhang S,Liu X,Jiang Q,Ding W

更新日期：2018-11-01 00:00:00

abstract：:A novel fluoro-containing jasmonate derivative was chemically synthesized and evaluated as a potential elicitor with respect to the induction of plant defense responses and the biosynthesis of plant secondary metabolites. A bioactive taxuyunnanine C (Tc)-producing cell line of Taxus chinensis was taken as a model plan...

journal_title：Applied microbiology and biotechnology

authors： Qian ZG,Zhao ZJ,Xu Y,Qian X,Zhong JJ

更新日期：2005-07-01 00:00:00