Construction of a new host-vector system in Arthrobacter sp. and cloning of the lipase gene.

abstract：:Cytophaga hutchinsonii is a Gram-negative aerobic soil bacterium which can digest crystalline cellulose completely through a strategy different from that of the well-studied cellulolytic aerobic fungi and anaerobic bacteria. However, despite the availability of a published genome sequence, studies on this organism hav...

journal_title：Applied microbiology and biotechnology

authors： Xu Y,Ji X,Chen N,Li P,Liu W,Lu X

更新日期：2012-01-01 00:00:00

abstract：:Aldolases are a specific group of lyases that catalyze the reversible stereoselective addition of a donor compound (nucleophile) onto an acceptor compound (electrophile). Whereas most aldolases are specific for their donor compound in the aldolization reaction, they often tolerate a wide range of aldehydes as acceptor...

journal_title：Applied microbiology and biotechnology

authors： Samland AK,Sprenger GA

更新日期：2006-07-01 00:00:00

abstract：:High vanillin productivity was achieved in the batch biotransformation of ferulic acid by Streptomyces sp. strain V-1. Due to the toxicity of vanillin and the product inhibition, fed-batch biotransformation with high concentration of ferulic acid was unsuccessful. To solve this problem and improve the vanillin yield, ...

journal_title：Applied microbiology and biotechnology

authors： Hua D,Ma C,Song L,Lin S,Zhang Z,Deng Z,Xu P

更新日期：2007-03-01 00:00:00

abstract：:Many different organic compounds may be converted by microbial biotransformation to high-value products for the chemical and pharmaceutical industries. This review summarizes the use of strains of Aspergillus niger, a well-known filamentous fungus used in numerous biotechnological processes, for biochemical transforma...

journal_title：Applied microbiology and biotechnology

authors： Parshikov IA,Woodling KA,Sutherland JB

更新日期：2015-09-01 00:00:00

abstract：:The dawn of a new Proteomics era, just over a decade ago, allowed for large-scale protein profiling studies that have been applied in the identification of distinctive molecular cell signatures. Proteomics provides a powerful approach for identifying and studying these multiple molecular markers in a vast array of bio...

journal_title：Applied microbiology and biotechnology

authors： da Costa JP,Carvalhais V,Ferreira R,Amado F,Vilanova M,Cerca N,Vitorino R

更新日期：2015-09-01 00:00:00

abstract：:To investigate how the microbial community in activated sludge responded to high antibiotic levels, a bench-scale aerobic wastewater treatment system was used to treat oxytetracycline (OTC) mother liquor (OTC-ML). Removal efficiency of chemical oxygen demand decreased from 64.9 to 51.0 % when the OTC level increased f...

journal_title：Applied microbiology and biotechnology

authors： Liu M,Zhang Y,Ding R,Gao Y,Yang M

更新日期：2013-10-01 00:00:00

abstract：:The complete biodegradation of azo dye, Fast Acid Red GR, was observed under microaerophilic conditions by Shewanella decolorationis S12. Although the highest decolorizing rate was measured under anaerobic condition and the highest biomass was obtained under aerobic condition, a further biodegradation of decolorizing ...

journal_title：Applied microbiology and biotechnology

authors： Xu M,Guo J,Sun G

更新日期：2007-09-01 00:00:00

abstract：:Infectious bursal disease is a widely spread threatening contagious viral infection of chickens that induces major damages to the Bursa of Fabricius and leads to severe immunosuppression in young birds causing significant economic losses for poultry farming. The etiological agent is the infectious bursal disease virus...

journal_title：Applied microbiology and biotechnology

authors： Rage E,Marusic C,Lico C,Baschieri S,Donini M

更新日期：2020-03-01 00:00:00

abstract：:An enzyme preparation from the thermophilic fungus Humicola insolens, Ultraflo L, was able to solubilise more than half of the biomass of brewer's grain and wheat bran, two agro-industrial co-products. While almost all of the ferulic acid was released in the free form, the majority of diferulates were released still a...

journal_title：Applied microbiology and biotechnology

authors： Faulds CB,Mandalari G,LoCurto R,Bisignano G,Waldron KW

更新日期：2004-06-01 00:00:00

abstract：:The TM1072 gene from Thermotoga maritima codifies for a putative form of a rhamnulose-1-phosphate aldolase (Rha-1PA Tm). To investigate this enzyme further, its gene was cloned and expressed in Escherichia coli. The purified enzyme was activated by Co(2+) as a divalent metal ion cofactor, instead of Zn(2+) as its E. c...

journal_title：Applied microbiology and biotechnology

authors： Oroz-Guinea I,Sánchez-Moreno I,Mena M,García-Junceda E

更新日期：2015-04-01 00:00:00

abstract：:A strong promoter increases transcription of the genes of interest and enhances the production of various valuable substances. For a hyperthermophilic archaeon Thermococcus onnurineus NA1, which can produce H2 from carbon monoxide oxidation, we searched for a novel endogenous strong promoter by transcriptome analysis ...

journal_title：Applied microbiology and biotechnology

authors： Lee SH,Kim MS,Jung HC,Lee J,Lee JH,Lee HS,Kang SG

更新日期：2015-05-01 00:00:00

abstract：:Probiotics and bovine colostrums had been proven to be beneficial for human health. Lactobacillus rhamnosus ZDY114 and anti-Helicobacter pylori bovine colostrums were used for the preparation of microecological additives, and their synergistic antidigestion effect in the simulated gastrointestinal tract (in vitro) was...

journal_title：Applied microbiology and biotechnology

authors： Wei H,Xu Y,Xiong Y,Xu F,Liu G

更新日期：2007-06-01 00:00:00

abstract：:Biodegradation can achieve complete and cost-effective elimination of aromatic pollutants through harnessing diverse microbial metabolic processes. Aromatics biodegradation plays an important role in environmental cleanup and has been extensively studied since the inception of biodegradation. These studies, however, a...

journal_title：Applied microbiology and biotechnology

authors： Cao B,Nagarajan K,Loh KC

更新日期：2009-11-01 00:00:00

abstract：:Wetlands contribute to 30% of global methane emissions due to an imbalance between microbial methane production and consumption. Methanogenesis and methanotrophy have mainly been studied separately, and little is known about their potential interactions in aquatic environments. To mimic the interaction between methane...

journal_title：Applied microbiology and biotechnology

authors： In 't Zandt MH,van den Bosch TJM,Rijkers R,van Kessel MAHJ,Jetten MSM,Welte CU

更新日期：2018-07-01 00:00:00

abstract：:In bioelectrochemical systems, exoelectrogenic bacteria respire with anode electrodes as their extracellular electron acceptor; therefore, lower anode potentials can reduce the energy gain to each microbe and select against ones that are not able to respire at a lower potential range. Often fully developed anode commu...

journal_title：Applied microbiology and biotechnology

authors： Yan H,Yates MD,Regan JM

更新日期：2015-11-01 00:00:00

abstract：:We in this study describe a new method for genomic studies of individual uncultured prokaryotic organisms, which was used for the isolation and partial genome sequencing of a soil archaeon. The diversity of Archaea in a soil sample was mapped by generating a clone library using group-specific primers in combination wi...

journal_title：Applied microbiology and biotechnology

authors： Kvist T,Ahring BK,Lasken RS,Westermann P

更新日期：2007-03-01 00:00:00

abstract：:Genome walking is a commonly used technique for the identification of DNA sequences adjacent to known regions. Despite the development of various genome walking methods, nonspecific products are often produced in certain circumstances, especially when GC-rich DNA sequences are dealt with. To effectively resolve such t...

journal_title：Applied microbiology and biotechnology

authors： Deng J,Wei M,Yu B,Chen Y

更新日期：2010-06-01 00:00:00

abstract：:The alpha-amylase gene (amy) from Streptomyces griseus IMRU 3570 and the beta-galactosidase gene (lac) from S. lividans were subcloned into Brevibacterium lactofermentum or B. lactofermentum/Escherichia coli shuttle vectors. The amy gene was not expressed in B. lactofermentum from its own promoter but was efficiently ...

journal_title：Applied microbiology and biotechnology

authors： Cadenas RF,Gil JA,Martín JF

更新日期：1992-12-01 00:00:00

abstract：:Six local isolates of yeasts were screened for cell mass and lipid production in mixed glucose and xylose medium. Candida tropicalis SY005 and Trichosporon (Apiotrichum) loubieri SY006 showed significant lipid accumulation of 24.6% and 32% (dry cell weight), respectively when grown in medium containing equal mass of b...

journal_title：Applied microbiology and biotechnology

authors： Chattopadhyay A,Maiti MK

更新日期：2020-04-01 00:00:00

abstract：:This study isolated a novel erythritol-producing yeast strain, which is capable of growth at high osmolarity. Characteristics of the strain include asexual reproduction by multilateral budding, absence of extracellular starch-like compounds, and a negative Diazonium blue B color reaction. Phylogenetic analysis based o...

journal_title：Applied microbiology and biotechnology

authors： Jeya M,Lee KM,Tiwari MK,Kim JS,Gunasekaran P,Kim SY,Kim IW,Lee JK

更新日期：2009-05-01 00:00:00

abstract：:Levulinic acid was esterified with methanol, ethanol, and 1-butanol with the final goal to predict the maximum yield of these equilibrium-limited reactions as function of medium composition. In a first step, standard reaction data (standard Gibbs energy of reaction Δ R g 0 ) were determined from experimental formation...

journal_title：Applied microbiology and biotechnology

authors： Altuntepe E,Emel'yanenko VN,Forster-Rotgers M,Sadowski G,Verevkin SP,Held C

更新日期：2017-10-01 00:00:00

abstract：:The principal point of this paper is that the discovery of penicillin and the development of the supporting technologies in microbiology and chemical engineering leading to its commercial scale production represent it as the medicine with the greatest impact on therapeutic outcomes. Our nomination of penicillin for th...

journal_title：Applied microbiology and biotechnology

authors： Kardos N,Demain AL

更新日期：2011-11-01 00:00:00

abstract：:Two extracellular enzymes (MsP1 and MsP2) capable of efficient beta-carotene degradation were purified from culture supernatants of the basidiomycete Marasmius scorodonius (garlic mushroom). Under native conditions, the enzymes exhibited molecular masses of approximately 150 and approximately 120 kDa, respectively. SD...

journal_title：Applied microbiology and biotechnology

authors： Scheibner M,Hülsdau B,Zelena K,Nimtz M,de Boer L,Berger RG,Zorn H

更新日期：2008-01-01 00:00:00

abstract：:Based on natural selection and the survival of the fittest by evolutionary adaption, a smart high-throughput system was developed to select active haloalkane dehalogenase variants from a large mutant library. Only active enzyme variants can hydrolyse toxic halogenated alkanes to promote growth, whereas inactive mutant...

journal_title：Applied microbiology and biotechnology

authors： Fibinger MP,Davids T,Böttcher D,Bornscheuer UT

更新日期：2015-11-01 00:00:00

abstract：:Production of volatile sulphur compounds (VSC) was assessed in culture media supplemented with L-methionine or L-methionine/L-cysteine mixtures, using five cheese-ripening yeasts: Debaryomyces hansenii DH47(8), Kluyveromyces lactis KL640, Geotrichum candidum GC77, Yarrowia lipolytica YL200 and Saccharomyces cerevisiae...

journal_title：Applied microbiology and biotechnology

authors： López Del Castillo-Lozano M,Delile A,Spinnler HE,Bonnarme P,Landaud S

更新日期：2007-07-01 00:00:00

abstract：:In this work, the production of 1,3-propanediol from glucose and molasses was studied in a two-step process using two recombinant microorganisms. The first step of the process is the conversion of glucose or other sugar into glycerol by the metabolic engineered Saccharomyces cerevisiae strain HC42 adapted to high (>20...

journal_title：Applied microbiology and biotechnology

authors： Mendes FS,González-Pajuelo M,Cordier H,François JM,Vasconcelos I

更新日期：2011-11-01 00:00:00

abstract：:Fat, oil and, grease (FOG) deposits are one primary cause of sanitary sewer overflows (SSOs). While numerous studies have examined the formation of FOG deposits in sewer pipes, little is known about their biodegradation under sewer environments. In this study, FOG deposit biodegradation potential was determined by stu...

journal_title：Applied microbiology and biotechnology

authors： He X,Zhang Q,Cooney MJ,Yan T

更新日期：2015-07-01 00:00:00

abstract：:Ene-reductases originating from extremophiles are gaining importance in the field of biocatalysis due to higher-stability properties. The genome of the acidophilic iron-oxidizing bacterium "Ferrovum" sp. JA12 was found to harbor a thermophilic-like ene-reductase (FOYE-1). The foye-1 gene was ligated into a pET16bp exp...

journal_title：Applied microbiology and biotechnology

authors： Scholtissek A,Ullrich SR,Mühling M,Schlömann M,Paul CE,Tischler D

更新日期：2017-01-01 00:00:00

abstract：:Nanotechnology is a promising field of interdisciplinary research. It opens up a wide array of opportunities in various fields like medicine, pharmaceuticals, electronics and agriculture. The potential uses and benefits of nanotechnology are enormous. These include insect pests management through the formulations of n...

journal_title：Applied microbiology and biotechnology

authors： Rai M,Ingle A

更新日期：2012-04-01 00:00:00

abstract：:Smokeless tobacco (ST) products are used worldwide and are a major public health concern. In addition to harmful chemicals found in these products, microbes found in ST products are believed to be responsible for generating harmful tobacco-specific nitrosamines (TSNAs), the most abundant carcinogens in ST. These micro...

journal_title：Applied microbiology and biotechnology

authors： Tyx RE,Rivera AJ,Keong LM,Stanfill SB

更新日期：2020-01-01 00:00:00