Abstract：
:We report the successful high-yield expression of Candida utilis uricase in Escherichia coli and the establishment of an efficient three-step protein purification protocol. The purity of the recombinant protein, which was confirmed to be C. utilis uricase by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometer analysis, was >98% and the specific activity was 38.4 IU/mg. Crystals of C. utilis uricase were grown at 18°C using 25% polyethylene glycol 3350 as precipitant. Diffraction by the crystals extends to 1.93 Å resolution, and the crystals belong to the space group P2(1)2(1)2(1) with unit cell parameters a = 69.16 Å, b = 139.31 Å, c = 256.33 Å, and α = β = γ = 90°. The crystal structure of C. utilis uricase shares a high similarity with other reported structures of the homologous uricases from other species in protein database, demonstrating that the three-dimensional structure of the protein defines critically to the catalytic activities.
journal_name
Appl Microbiol Biotechnoljournal_title
Applied microbiology and biotechnologyauthors
Liu X,Wen M,Li J,Zhai F,Ruan J,Zhang L,Li Sdoi
10.1007/s00253-011-3244-0subject
Has Abstractpub_date
2011-11-01 00:00:00pages
529-37issue
3eissn
0175-7598issn
1432-0614journal_volume
92pub_type
杂志文章abstract：:The existence of taste and odour (T&O) in drinking water is one of the principal causes of consumer complaints and is commonly related to algae growth. Numerous studies have confirmed the existence of algal blooms emerging specifically in low-temperature periods, herein referred to as "cold algae"; these include chrys...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s00253-020-10884-6
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abstract：:DNA microarrays have found widespread use as a flexible tool to investigate bacterial metabolism. Their main advantage is the comprehensive data they produce on the transcriptional response of the whole genome to an environmental or genetic stimulus. This allows the microbiologist to monitor metabolism and to define s...
journal_title：Applied microbiology and biotechnology
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abstract：:Bacillus thuringiensis bacteria show insecticidal activities that rely upon the production of insecticidal crystal proteins, which are encoded by cry or cyt genes and can target a variety of insect pests. It has been shown that cry1Ac is the only cry gene in B. thuringiensis subsp. kurstaki HD73 (B. thuringiensis HD73...
journal_title：Applied microbiology and biotechnology
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journal_title：Applied microbiology and biotechnology
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journal_title：Applied microbiology and biotechnology
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doi：10.1007/BF00172722
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pub_type： 杂志文章
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更新日期：2015-06-01 00:00:00
abstract：:The published online version contains editing mistake in Table 2. See below for the corrected Table. ...
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更新日期：2018-01-01 00:00:00
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更新日期：2012-01-01 00:00:00
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journal_title：Applied microbiology and biotechnology
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pub_type： 杂志文章
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journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
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journal_title：Applied microbiology and biotechnology
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