Molecular cloning in Lactobacillus helveticus by plasmid pSA3::pVA797 co-integrate formation and conjugal transfer.

abstract：:Increased pentose phosphate pathway flux, relative to total substrate uptake flux, is shown to enhance succinic acid (SA) yields under continuous, non-growth conditions of Actinobacillus succinogenes biofilms. Separate fermentations of glucose and xylose were conducted in a custom, continuous biofilm reactor at four d...

journal_title：Applied microbiology and biotechnology

authors： Bradfield MF,Nicol W

更新日期：2016-11-01 00:00:00

abstract：:Deoxynucleoside-5'-monophosphates (5'-dNMPs) are the basic components of DNA and are widely used in medicine and as chemical and biochemical reagents. A large amount of effort has been expended to obtain 5'-dNMPs of high quality and at a low cost. However, these procedures are inefficient and inconvenient. In this stu...

journal_title：Applied microbiology and biotechnology

authors： Zou Z,Ding Q,Ou L,Yan B

更新日期：2013-11-01 00:00:00

abstract：:Rapid sugar consumption is important for the microbial production of chemicals and fuels. Here, we show that overexpression of the NADH dehydrogenase gene (ndh) increased glucose consumption rate in Corynebacterium glutamicum under oxygen-deprived conditions through investigating the relationship between the glucose c...

journal_title：Applied microbiology and biotechnology

authors： Tsuge Y,Uematsu K,Yamamoto S,Suda M,Yukawa H,Inui M

更新日期：2015-07-01 00:00:00

abstract：:Bacillus cereus 905, one of the plant growth-promoting rhizobacteria (PGPRs), is capable of colonizing wheat roots in a large population size. From previous studies, we learned that the sodA2-encoding manganese-containing superoxide dismutase (MnSOD2) is important for B. cereus 905 to survive in wheat rhizosphere. In ...

journal_title：Applied microbiology and biotechnology

authors： Gao T,Ding M,Wang Q

更新日期：2020-11-01 00:00:00

abstract：:Trypsin is commonly used in Madin-Darby canine kidney (MDCK) cell culture-based influenza vaccine production to facilitate virus infection by proteolytic activation of viral haemagglutinin, which enables multi-cycle replication. In this study, we were able to demonstrate that trypsin also interferes with pathogen defe...

journal_title：Applied microbiology and biotechnology

authors： Seitz C,Isken B,Heynisch B,Rettkowski M,Frensing T,Reichl U

更新日期：2012-01-01 00:00:00

abstract：:Corynebacterium glutamicum, a gram-positive soil bacterium, has been regarded as an aerobe because its growth by fermentative catabolism or by anaerobic respiration has, to this date, not been demonstrated. In this study, we report on the anaerobic growth of C. glutamicum in the presence of nitrate as a terminal elect...

journal_title：Applied microbiology and biotechnology

authors： Nishimura T,Vertès AA,Shinoda Y,Inui M,Yukawa H

更新日期：2007-06-01 00:00:00

abstract：:The basidiomycete Cystoderma carcharias transformed citronellol into 3,7-dimethyl-1,6,7-octanetriol as the main product. 3,7-Dimethyl-6,7-epoxy-1-octanol was identified as important intermediary product of the biotransformation, and the allylic diols 2,6-dimethyl-2-octene-1,8-diol, 3,7-dimethyl-5-octene-1,7-diol and 3...

journal_title：Applied microbiology and biotechnology

authors： Onken J,Berger RG

更新日期：1999-02-01 00:00:00

abstract：:A recombinant form of the sweet-tasting protein thaumatin has been produced in the filamentous fungus Aspergillus niger var. awamori. Expression cassettes containing a synthetic gene encoding thaumatin II were prepared and used to transform Aspergillus niger var. awamori strain NRRL312. Several fungal strains capable ...

journal_title：Applied microbiology and biotechnology

authors： Faus I,del Moral C,Adroer N,del Río JL,Patiño C,Sisniega H,Casas C,Bladé J,Rubio V

更新日期：1998-04-01 00:00:00

abstract：:A novel system was developed for efficient transformation of the fungal biocontrol agent Beauveria bassiana. Competent blastospores were prepared and stored in LiAc- and glycerol-inclusive suspension at -76 degrees C for sequential use in transformation. The system was successfully applied to integrating phosphinothri...

journal_title：Applied microbiology and biotechnology

authors： Ying SH,Feng MG

更新日期：2006-08-01 00:00:00

abstract：:Decolorisation of azo dyes during biological effluent treatment can involve both adsorption to cell biomass and degradation by azo-bond reduction during anaerobic digestion. Degradation is expected to form aromatic amines, which may be toxic and recalcitrant to anaerobic treatment but degradable aerobically. Methods f...

journal_title：Applied microbiology and biotechnology

authors： O'Neill C,Lopez A,Esteves S,Hawkes FR,Hawkes DL,Wilcox S

更新日期：2000-02-01 00:00:00

abstract：:Phytases are important industrial enzymes able to catalyze the release of up to six phosphates from phytate in a stepwise hydrolysis reaction. Phytases are almost exclusively used as a feed supplement. However, phytases are also used in human nutrition, food processing, non-food industrial products, and emerging appli...

journal_title：Applied microbiology and biotechnology

authors： Herrmann KR,Ruff AJ,Infanzón B,Schwaneberg U

更新日期：2019-08-01 00:00:00

abstract：:Corynebacterium glutamicum, a high GC content gram-positive soil bacterium in Actinobacteria, has been used for the industrial production of amino acids and engineered to produce various compounds, including polymer building blocks and biofuels. Since its genome sequence was first published, its versatile metabolic pa...

journal_title：Applied microbiology and biotechnology

authors： Toyoda K,Inui M

更新日期：2016-01-01 00:00:00

abstract：:Genetic engineering of transcription factors is an efficient strategy to improve lignocellulolytic enzyme production in fungi. In this study, the xylanase transcriptional regulators of Trichoderma reesei (Xyr1) and Neurospora crassa (XLR-1), as well as their constitutively active mutants (Xyr1A824V and XLR-1A828V), we...

journal_title：Applied microbiology and biotechnology

authors： Xia C,Li Z,Xu Y,Yang P,Gao L,Yan Q,Li S,Wang Y,Qu Y,Song X

更新日期：2019-03-01 00:00:00

abstract：:Mitochondrial dysfunction in Saccharomyces cerevisiae was selected as a marker of ion penetration following carbon ion beam (CIB) irradiation. Respiration-deficient mutants were screened. Following confirmation of negligible spontaneous mutation, eight genetically stable S. cerevisiae respiration-deficient mutant stra...

journal_title：Applied microbiology and biotechnology

authors： Guo X,Zhang M,Gao Y,Cao G,Yang Y,Lu D,Li W

更新日期：2019-02-01 00:00:00

abstract：:Myxobacteria are Gram-negative soil microorganisms that prey on other microorganisms. Myxobacteria have significant potential for applications in biotechnology because of their extraordinary ability to produce natural products such as secondary metabolites. Myxobacteria also stand out as model organisms for the study ...

journal_title：Applied microbiology and biotechnology

authors： Ueki T,Inouye S

更新日期：2006-08-01 00:00:00

abstract：:Serratia marcescens has been proved to be a potential strain for industrial 2,3-butanediol production for its high yield, productivity, and other advantages. In this study, the genes slaA, slaB, slaC, and slaR were successfully cloned which were further confirmed to be encoding acetolactate decarboxylase, acetolactate...

journal_title：Applied microbiology and biotechnology

authors： Rao B,Zhang LY,Sun J,Su G,Wei D,Chu J,Zhu J,Shen Y

更新日期：2012-03-01 00:00:00

abstract：:A cytochrome P450 and an iron-sulfur protein, whose expression was specifically induced during growth of Mycobacterium sp. strain HE5 on morpholine as the sole source of carbon, nitrogen, and energy were purified to apparent homogeneity. Due to the lack of enzymatic activity, carbon monoxide difference spectra and det...

journal_title：Applied microbiology and biotechnology

authors： Sielaff B,Andreesen JR,Schräder T

更新日期：2001-08-01 00:00:00

abstract：:Over 9,000 flavonoid compounds have been found in various plants, comprising one of the largest families of natural products. Flavonoids are an essential factor in plant interactions with the environment, often serving as the first line of defense against UV irradiation and pathogen attacks. Flavonoids are also major ...

journal_title：Applied microbiology and biotechnology

authors： Wang Y,Chen S,Yu O

更新日期：2011-08-01 00:00:00

abstract：:Time-resolved analysis assays of receptor-ligand interactions are fundamental in basic research and drug discovery. Adequate methods are well developed for the analysis of recombinant proteins such as antibody-antigen interactions. However, assays for time-resolved ligand-binding processes on living cells are still ra...

journal_title：Applied microbiology and biotechnology

authors： Encarnação JC,Schulte T,Achour A,Björkelund H,Andersson K

更新日期：2018-05-01 00:00:00

abstract：:β-Glucuronidase is a lysosomal enzyme and a molecular model of a class of therapeutics approved as enzyme replacement therapies for lysosomal storage diseases. Understanding the effect of bioreactor process variables on the production and quality of the biologics is critical for maintaining quality and efficacy of the...

journal_title：Applied microbiology and biotechnology

authors： Parhiz H,Ketcham SA,Zou G,Ghosh B,Fratz-Berilla EJ,Ashraf M,Ju T,Madhavarao CN

更新日期：2019-08-01 00:00:00

abstract：:The hydrolysis of cellulose into fermentable sugars is a costly and rate-limiting step in the production of biofuels from renewable feedstocks. Developing new cellulase systems capable of increased cellulose hydrolysis rates would reduce biofuel production costs. With this in mind, we screened 55 fungal endoglucanases...

journal_title：Applied microbiology and biotechnology

authors： Tambor JH,Ren H,Ushinsky S,Zheng Y,Riemens A,St-Francois C,Tsang A,Powlowski J,Storms R

更新日期：2012-01-01 00:00:00

abstract：:Enantioselective oxidation of racemic phenyl-1,2-ethanediol into (R)-(-)-mandelic acid by a newly isolated Brevibacterium lutescens CCZU12-1 was demonstrated. It was found that optically active (R)-(-)-mandelic acid (e.e.p > 99.9 %) is produced leaving the other enantiomer (S)-(+)-phenyl-1,2-ethanediol intact. Using f...

journal_title：Applied microbiology and biotechnology

authors： He YC,Ma CL,Zhang X,Li L,Xu JH,Wu MX

更新日期：2013-08-01 00:00:00

abstract：:Cytophaga hutchinsonii is a Gram-negative aerobic soil bacterium which can digest crystalline cellulose completely through a strategy different from that of the well-studied cellulolytic aerobic fungi and anaerobic bacteria. However, despite the availability of a published genome sequence, studies on this organism hav...

journal_title：Applied microbiology and biotechnology

authors： Xu Y,Ji X,Chen N,Li P,Liu W,Lu X

更新日期：2012-01-01 00:00:00

abstract：:Cupriavidus basilensis is a species with diverse metabolic capabilities, including degradation of xenobiotics and heavy metal resistance. Although the genomes of several strains of this species have been sequenced, no plasmid has yet been constructed for genetic engineering in this species. In this study, we identifie...

journal_title：Applied microbiology and biotechnology

authors： Wang S,Zhang MN,Bai NL,Ding HT,Zhu XF,Zhao YH

更新日期：2017-02-01 00:00:00

abstract：:An isolated bacterium that converted unsaturated fatty acids to hydroxy fatty acids was identified as Stenotrophomonas nitritireducens by API analysis, cellular fatty acids compositions, sequencing the full 16S ribosomal ribonucleic acid, and evaluating its nitrite reduction ability. S. nitritireducens has unique regi...

journal_title：Applied microbiology and biotechnology

authors： Yu IS,Yeom SJ,Kim HJ,Lee JK,Kim YH,Oh DK

更新日期：2008-02-01 00:00:00

abstract：:The 3D structures of Fim H and PapG proteins complexed with the host carbohydrate receptor demonstrate that both utilize binding-pocket asparagines for contact or stabilization with the carbohydrate. Pretreatment of whole bacteria with asparaginase resulted in decreased fimbriae-mediated attachment to urinary epitheli...

journal_title：Applied microbiology and biotechnology

authors： Wehner MR,Koch SR,Kindinger L,Nelson N,Cowan MM

更新日期：2004-11-01 00:00:00

abstract：:Agar is a major cell wall carbohydrate of red macroalgae (Rhodophyta). Sugars derived from agar, such as agarooligosaccharides (AOSs), neoagarooligosaccharides (NAOSs), neoagarobiose (NAB), and 3,6-anhydro-L-galactose (L-AHG), possess various physiological activities. These agar-derived sugars can be produced by hydro...

journal_title：Applied microbiology and biotechnology

authors： Yun EJ,Yu S,Kim KH

更新日期：2017-07-01 00:00:00

abstract：:The sheep rumen microbial community plays an important role in animal performance and the environment. Few studies have paid close attention to the impact of different levels of dietary nutrition on rumen microbial populations. A total of 112 healthy Tan sheep were selected and randomly allotted to one of four dietary...

journal_title：Applied microbiology and biotechnology

authors： Wang Y,Cao P,Wang L,Zhao Z,Chen Y,Yang Y

更新日期：2017-05-01 00:00:00

abstract：:River-bay system is a transitional zone connecting land and ocean and an important natural source for methane emission. Methanogens play important roles in the global greenhouse gas budget and carbon cycle since they produce methane. The abundance and community assemblage of methanogens in such a dynamic system are no...

journal_title：Applied microbiology and biotechnology

authors： Zhang CJ,Chen YL,Pan J,Wang YM,Li M

更新日期：2020-05-01 00:00:00

abstract：:A recombinant oleate hydratase from Lysinibacillus fusiformis converted ricinoleic acid to a product, whose chemical structure was identified as the novel compound 10,12-dihydroxystearic acid by gas chromatograph/mass spectrometry, Fourier transform infrared, and nuclear magnetic resonance analysis. The reaction condi...

journal_title：Applied microbiology and biotechnology

authors： Seo MH,Kim KR,Oh DK

更新日期：2013-10-01 00:00:00