Trypsin promotes efficient influenza vaccine production in MDCK cells by interfering with the antiviral host response.

abstract：:By employing living cells of the green alga Chlamydomonas reinhardtii, we demonstrate the possibility of direct electricity generation from microbial photosynthetic activity. The presented concept is based on an in situ oxidative depletion of hydrogen, photosynthetically produced by C. reinhardtii under sulfur-deprive...

journal_title：Applied microbiology and biotechnology

authors： Rosenbaum M,Schröder U,Scholz F

更新日期：2005-10-01 00:00:00

abstract：:Engineered S. cerevisiae employing the xylose reductase pathway enables efficient xylose valorization to fuels and chemicals. However, toxicity of thermochemically pretreated biomass hydrolysate on S. cerevisiae is one of the key technical challenges to upgrade biomass-derived sugars including xylose and glucose into ...

journal_title：Applied microbiology and biotechnology

authors： Jayakody LN,Turner TL,Yun EJ,Kong II,Liu JJ,Jin YS

更新日期：2018-09-01 00:00:00

abstract：:We have fused the epidermal growth factor (EGF) to the amino terminus of Pseudomonas exotoxin A (PE) to create a cytotoxic agent, designated EGF-PE, which preferentially kills EGF-receptor-bearing cells. In this study, we analyzed the effect of the Ia domain, the binding domain of PE on the cytotoxicity of EGF-PE towa...

journal_title：Applied microbiology and biotechnology

authors： Liao CW,Hseu TH,Hwang J

更新日期：1995-07-01 00:00:00

abstract：:This review addresses metal uptake specifically by yeast. Metal uptake may be passive, active or both, depending on the viability of the biomass, and is influenced by a number of environmental and experimental factors. Uptake is typically accompanied by a degree of ion exchange and, under certain conditions, may be en...

journal_title：Applied microbiology and biotechnology

authors： Blackwell KJ,Singleton I,Tobin JM

更新日期：1995-08-01 00:00:00

abstract：:Nitric oxide (NO) is known to be involved in modulating production of styrylpyrone polyphenols in the basidiomycete Inonotus obliquus. However, it remains unknown how NO orchestrates fungal styrylpyrone biosynthesis. Here, we show that a transient NO burst correlated with an enhanced expression of phenylalanine ammoni...

journal_title：Applied microbiology and biotechnology

authors： Zhao Y,He M,Xi Q,Ding J,Hao B,Keller NP,Zheng W

更新日期：2016-05-01 00:00:00

abstract：:Inactivation of D-amino acid oxidase occurred by different mechanisms. The enzyme showed a rapid loss of activity in the presence of micromolar amounts of Cu2+ and Hg2+. It was also sensitive to oxidative inactivation by Fe2+ and H2O2 when both reagents were added in millimolar amounts. When oxidatively inactivated D-...

journal_title：Applied microbiology and biotechnology

authors： Schräder T,Andreesen JR

更新日期：1996-05-01 00:00:00

abstract：:Entomopathogenic fungi are widely produced for use as mycoinsecticides. Therefore, improvement of the shelf life of fungal propagules under good and adverse conditions should be a pre-requisite of their production. In order to improve conidial physiology as well as mycoinsecticide efficiency, culture conditions may be...

journal_title：Applied microbiology and biotechnology

authors： Tarocco F,Lecuona RE,Couto AS,Arcas JA

更新日期：2005-09-01 00:00:00

abstract：:Enzymatic production of biodiesel by transesterification of triglycerides and alcohol, catalyzed by lipases, offers an environmentally friendly and efficient alternative to the chemically catalyzed process while using low-grade feedstocks. Methanol is utilized frequently as the alcohol in the reaction due to its react...

journal_title：Applied microbiology and biotechnology

authors： Dror A,Kanteev M,Kagan I,Gihaz S,Shahar A,Fishman A

更新日期：2015-11-01 00:00:00

abstract：:Phytophthora infestans is the causative agent of potato blight that resulted in the great famine in Ireland in the nineteenth century. This microbe can release large amounts of the C20 very long-chain polyunsaturated fatty acids arachidonic acid (ARA; 20:4Δ(5, 8, 11, 14)) and eicosapentaenoic acid (EPA; 20:5Δ(5, 8, 11...

journal_title：Applied microbiology and biotechnology

authors： Sun Q,Liu J,Zhang Q,Qing X,Dobson G,Li X,Qi B

更新日期：2013-09-01 00:00:00

abstract：:Levulinic acid was esterified with methanol, ethanol, and 1-butanol with the final goal to predict the maximum yield of these equilibrium-limited reactions as function of medium composition. In a first step, standard reaction data (standard Gibbs energy of reaction Δ R g 0 ) were determined from experimental formation...

journal_title：Applied microbiology and biotechnology

authors： Altuntepe E,Emel'yanenko VN,Forster-Rotgers M,Sadowski G,Verevkin SP,Held C

更新日期：2017-10-01 00:00:00

abstract：:Two bioactive O-methylflavonoids, sakuranetin (7-O-methylnaringenin) and ponciretin (7-O-methylnaringenin), were synthesized in Escherichia coli. Sakuranetin inhibits germination of Magnaporthe grisea, and ponciretin is a potential inhibitor of Helicobacter pylori. To achieve this, we reconstructed the naringenin bios...

journal_title：Applied microbiology and biotechnology

authors： Kim MJ,Kim BG,Ahn JH

更新日期：2013-08-01 00:00:00

abstract：:This research investigated the effect of microwave irradiation (MWI) on cell disintegration in municipal secondary sludge (MSS). A representative MSS Gram-positive bacterium (Bacillus subtilis) and Gram-negative bacteria (Acinetobacter calcoaceticus and Pseudomonas aeruginosa) were pure cultured separately and treated...

journal_title：Applied microbiology and biotechnology

authors： Zhou BW,Shin SG,Hwang K,Ahn JH,Hwang S

更新日期：2010-06-01 00:00:00

abstract：:The pathogenicity of Salmonella Typhimurium, a foodborne pathogen, is mainly attributed to its ability to form biofilm on food contact surfaces. ε-polylysine, a polymer of positively charged lysine, is reported to inhibit biofilm formation of both gram-positive and gram-negative bacteria. To elucidate the mechanism un...

journal_title：Applied microbiology and biotechnology

authors： Shen C,Islam MT,Masuda Y,Honjoh KI,Miyamoto T

更新日期：2020-06-01 00:00:00

abstract：:Inteins are internal protein elements that self-excise from their host protein and catalyze ligation of the flanking sequences (exteins) with a peptide bond. They are found in organisms in all three domains of life, and in viral proteins. Intein excision is a posttranslational process that does not require auxiliary e...

journal_title：Applied microbiology and biotechnology

authors： Elleuche S,Pöggeler S

更新日期：2010-06-01 00:00:00

abstract：:To meet the demand for sustainable energy, aquifer thermal energy storage (ATES) is widely used in the subsurface in urban areas. However, contamination of groundwater, especially with chlorinated volatile organic compounds (CVOCs), is often being encountered. This is commonly seen as an impediment to ATES implementat...

journal_title：Applied microbiology and biotechnology

authors： Ni Z,van Gaans P,Smit M,Rijnaarts H,Grotenhuis T

更新日期：2016-04-01 00:00:00

abstract：:Coxsackievirus A16 (CA16) is one of the major causative agents of hand, foot, and mouth disease (HFMD). No CA16 vaccine candidates have progressed to clinical trials so far. Immunogenicity studies indicated that different CA16 particles have much influence on the efficacy of a candidate vaccine. However, there are sti...

journal_title：Applied microbiology and biotechnology

authors： Ye X,Yang L,Jia J,Han J,Li S,Liu Y,Xu L,Zhao H,Chen Y,Li Y,Cheng T,Xia N

更新日期：2016-03-01 00:00:00

abstract：:The Bacillus subtilis lipoprotein PrsA enhances the yield of several homologous and heterologous exported proteins in B. subtilis by being involved in the posttranslocational stage of the secretion process. In this work, we have studied the effect of B. subtilis PrsA on the secretion of Bacillus amyloliquefaciens alph...

journal_title：Applied microbiology and biotechnology

authors： Lindholm A,Ellmén U,Tolonen-Martikainen M,Palva A

更新日期：2006-12-01 00:00:00

abstract：:A 6-kDa antifungal peptide with inhibitory activity on mycelial growth in Fusarium oxysporum, Mycosphaerella arachidicola, and Physalospora piricola was isolated from baby lima beans. The peptide suppressed growth in M. arachidicola with an IC(50) of 0.87 muM and inhibited activity of HIV-1 reverse transcriptase with ...

journal_title：Applied microbiology and biotechnology

authors： Wang HX,Ng TB

更新日期：2006-12-01 00:00:00

abstract：:To investigate how the microbial community in activated sludge responded to high antibiotic levels, a bench-scale aerobic wastewater treatment system was used to treat oxytetracycline (OTC) mother liquor (OTC-ML). Removal efficiency of chemical oxygen demand decreased from 64.9 to 51.0 % when the OTC level increased f...

journal_title：Applied microbiology and biotechnology

authors： Liu M,Zhang Y,Ding R,Gao Y,Yang M

更新日期：2013-10-01 00:00:00

abstract：:The alpha-amylase gene (amy) from Streptomyces griseus IMRU 3570 and the beta-galactosidase gene (lac) from S. lividans were subcloned into Brevibacterium lactofermentum or B. lactofermentum/Escherichia coli shuttle vectors. The amy gene was not expressed in B. lactofermentum from its own promoter but was efficiently ...

journal_title：Applied microbiology and biotechnology

authors： Cadenas RF,Gil JA,Martín JF

更新日期：1992-12-01 00:00:00

abstract：:The alpha-amylase of Streptomyces sp. IMD 2679 was subject to catabolite repression. Four different growth rates were achieved when the organism was grown at 40 degrees C and 55 degrees C in the presence and absence of cobalt, with an inverse relationship between alpha-amylase production and growth rate. Highest alpha...

journal_title：Applied microbiology and biotechnology

authors： Mc Mahon HE,Kelly CT,Fogarty WM

更新日期：1997-10-01 00:00:00

abstract：:The suitability of culture supernatant from Streptomyces albus ATCC 3005 for use in the biobleaching of eucalyptus kraft pulp was investigated. S. albus was found to grow on a minimal salts medium containing oat spelts xylan and yeast extract as the main carbon and nitrogen sources, respectively. Maximal extracellular...

journal_title：Applied microbiology and biotechnology

authors： Antonopoulos VT,Hernandez M,Arias ME,Mavrakos E,Ball AS

更新日期：2001-10-01 00:00:00

abstract：:Cyanobacterial blooms have become a serious problem in Lake Taihu during the last 20 years, and Microcystis aeruginosa and Synechococcus sp. are the two dominant species in cyanobacterial blooms of Lake Taihu. A freshwater bacterial strain, Shewanella sp. Lzh-2, with strong algicidal properties against harmful cyanoba...

journal_title：Applied microbiology and biotechnology

authors： Li Z,Lin S,Liu X,Tan J,Pan J,Yang H

更新日期：2014-05-01 00:00:00

abstract：:Traditional temperature-sensitive systems use either heat shock (40-42 °C) or cold shock (15-23 °C) to induce gene expression at temperatures that are not the optimal temperature for host cell growth (37 °C). This impacts the overall productivity and yield by disturbing cell growth and cellular metabolism. Here, we ha...

journal_title：Applied microbiology and biotechnology

authors： Jiang X,Zhang H,Yang J,Liu M,Feng H,Liu X,Cao Y,Feng D,Xian M

更新日期：2013-06-01 00:00:00

abstract：:(S)-Hydroxynitrile lyase from Manihot esculenta (MeHNL) was shown for the first time to be able to catalyze the enantioselective transcyanation of acetyltrimethylsilane (ATMS) with acetone cyanohydrin to form (S)-2-trimethylsilyl-2-hydroxyl-propionitrile in an aqueous/organic biphasic system. To better understand the ...

journal_title：Applied microbiology and biotechnology

authors： Xu R,Zong MH,Liu YY,He J,Zhang YY,Lou WY

更新日期：2004-11-01 00:00:00

abstract：:The catalytic domain encoded by an adenine-thymine (AT)-rich xylanase gene (xynA) of the anaerobic fungus Orpinomyces was expressed in Hypocrea jecorina under the control of the cel7A promoter and terminator. No XynA protein was detected in H. jecorina culture supernatants when the original sequence was fused to the H...

journal_title：Applied microbiology and biotechnology

authors： Li XL,Skory CD,Ximenes EA,Jordan DB,Dien BS,Hughes SR,Cotta MA

更新日期：2007-04-01 00:00:00

abstract：:To improve the stability and catalytic efficiency of pyranose 2-oxidase (P2Ox) by molecular enzyme evolution, we cloned P2Ox cDNA by RACE-PCR from a cDNA library derived from the basidiomycete Peniophora gigantea. The P2Ox gene was expressed in Escherichia coli BL21(DE3), yielding an intracellular and enzymatically ac...

journal_title：Applied microbiology and biotechnology

authors： Bastian S,Rekowski MJ,Witte K,Heckmann-Pohl DM,Giffhorn F

更新日期：2005-06-01 00:00:00

abstract：:Phomopsis sp. XP-8 is an endophytic fungus that has the ability to produce pinoresinol diglucoside (PDG) in vitro and thus has potential application for the biosynthesis of PDG independent of plants. When cultivated in mung bean medium, PDG production was significantly improved and pinoresinol monoglucoside (PMG) and ...

journal_title：Applied microbiology and biotechnology

authors： Zhang Y,Shi J,Gao Z,Yangwu R,Jiang H,Che J,Liu Y

更新日期：2015-06-01 00:00:00

abstract：:L-Malic acid and fumaric acid are C4 dicarboxylic organic acids and considered as promising chemical building blocks. They can be applied as food preservatives and acidulants in rust removal and as polymerization starter units. Molds of the genus Aspergillus are able to produce malic acid in large quantities from gluc...

journal_title：Applied microbiology and biotechnology

authors： Ochsenreither K,Fischer C,Neumann A,Syldatk C

更新日期：2014-06-01 00:00:00

abstract：:Manipulation of product heterogeneity was attempted by using yeast extract as nitrogen source in Alternaria alternata S-f6 transformation process of 4'-demethylepipodophyllotoxin. When the nitrogen source of NaNO(3) was replaced by yeast extract, the heterogeneity of biotransformation products was significantly varied...

journal_title：Applied microbiology and biotechnology

authors： Zhao W,Li HM,Wan DJ,Tang YJ

更新日期：2012-01-01 00:00:00