Characterization of three novel desaturases involved in the delta-6 desaturation pathways for polyunsaturated fatty acid biosynthesis from Phytophthora infestans.

abstract：:Real-time polymerase chain reaction (RT-PCR) was used to quantify seven species of lactic acid bacteria (LAB) in alfalfa silage prepared in the presence or absence of four commercial inoculants and in uninoculated corn stover harvested and stored under a variety of field conditions. Species-specific PCR primers were d...

journal_title：Applied microbiology and biotechnology

authors： Stevenson DM,Muck RE,Shinners KJ,Weimer PJ

更新日期：2006-07-01 00:00:00

abstract：:6-Oxopiperidine-2-carboxylic acid (OCA; cyclic alpha-aminoadipic acid) reverses the L-lysine inhibition of penicillin G production by Penicillium chrysogenum PQ-96. The reaction probably depends on the recovery of L-alpha-aminoadipic acid for penicillin G production from OCA. ...

journal_title：Applied microbiology and biotechnology

authors： Kurzatkowski W,Kurzatkowski JD,Filipek J,Solecka J,Kuryłowicz W

更新日期：1990-12-01 00:00:00

abstract：:Polyphosphosphate kinases (PPKs) catalyse the reversible transfer of the γ-phosphate group of a nucleoside-triphosphate to a growing chain of polyphosphate. Most known PPKs are specific for ATP, but some can also use GTP as a phosphate donor. In this study, we describe the properties of a PPK2-type PPK of the β-proteo...

journal_title：Applied microbiology and biotechnology

authors： Hildenbrand JC,Teleki A,Jendrossek D

更新日期：2020-08-01 00:00:00

abstract：:Drought, high/low temperature, and salinity are abiotic stress factors accepted as the main reason for crop yield losses in a world with growing population and food price increases. Additional problems create nutrient limitations and particularly low P soil status. The problem of phosphate fertilizers, P plant nutriti...

journal_title：Applied microbiology and biotechnology

authors： Vassilev N,Eichler-Löbermann B,Vassileva M

更新日期：2012-08-01 00:00:00

abstract：:Pseudomonads are ubiquitous Gram-negative gamma proteobacteria known for their extreme versatility and adaptability. Some are plant pathogens (Pseudomonas syringae) which have to survive on the surface of leaves while others can colonize the rhizosphere or survive in soil (Pseudomonas fluorescens, Pseudomonas putida),...

journal_title：Applied microbiology and biotechnology

authors： Cornelis P

更新日期：2010-05-01 00:00:00

abstract：:The marine photosynthetic bacterium Rhodovulum sulfidophilum produces extracellular nucleic acids involved in its flocculation. Previously, we showed that the RNA fraction of these extracellular nucleic acids released into the culture medium contains mainly non-aminoacylated fully mature-sized tRNAs and fragments of 1...

journal_title：Applied microbiology and biotechnology

authors： Suzuki H,Daimon M,Awano T,Umekage S,Tanaka T,Kikuchi Y

更新日期：2009-08-01 00:00:00

abstract：:A family I cellulose-binding domain (CBD) and a serine- and threonine-rich linker peptide were cloned from the fungi Aspergillus japonicus and Aspergillus aculeatus. A glutathione S-transferase (GST) fusion protein comprising GST and a peptide linker with the CBD fused to its C-terminus, was expressed in Escherichia c...

journal_title：Applied microbiology and biotechnology

authors： Quentin M,Ebbelaar M,Derksen J,Mariani C,van Der Valk H

更新日期：2002-04-01 00:00:00

abstract：:A novel fluoro-containing jasmonate derivative was chemically synthesized and evaluated as a potential elicitor with respect to the induction of plant defense responses and the biosynthesis of plant secondary metabolites. A bioactive taxuyunnanine C (Tc)-producing cell line of Taxus chinensis was taken as a model plan...

journal_title：Applied microbiology and biotechnology

authors： Qian ZG,Zhao ZJ,Xu Y,Qian X,Zhong JJ

更新日期：2005-07-01 00:00:00

abstract：:We have recently reported that expression of an unidentified heme protein is enhanced in a nitrifying activated sludge community under low (0.1 mg O2/L) dissolved oxygen (DO) conditions. A preliminary assessment suggested it may be a type of hemoglobin (Hb) or a lesser-known component of the energy-transducing pathway...

journal_title：Applied microbiology and biotechnology

authors： Arnaldos M,Kunkel SA,Stark BC,Pagilla KR

更新日期：2014-04-01 00:00:00

abstract：:The gene from Xanthomonas campestris pv. phaseoli that is involved in the C5 pathway of delta-amino-levulinic acid (ALA) of Escherichia coli. Subcloning of deletion fragments from the initial 2.5-kilobase (kb) chromosomal fragment allowed the isolation of a 1.6-kb fragment that could complement the hemM mutation. Nucl...

journal_title：Applied microbiology and biotechnology

authors： Asahara N,Murakami K,Korbrisate S,Hashimoto Y,Murooka Y

更新日期：1994-02-01 00:00:00

abstract：:The goal of sustainable production of biochemicals and biofuels has driven the engineering of microbial cell as factories that convert low-value substrates to high-value products. Xylose is the second most abundant sugar substrate in lignocellulosic hydrolysates. We analyzed the mechanisms of xylose metabolism using g...

journal_title：Applied microbiology and biotechnology

authors： Kim D,Woo HM

更新日期：2018-11-01 00:00:00

abstract：:This study examines the bioremediation potential and cadmium-induced cellular response on a molecular level in Candida tropicalis 3Aer. Spectroscopic analysis clearly illustrated the involvement of yeast cell wall components in biosorption. Cadmium bioaccumulation was confirmed by TEM, SEM, and EDX examination. TEM im...

journal_title：Applied microbiology and biotechnology

authors： Khan Z,Rehman A,Nisar MA,Zafar S,Hussain SZ,Zerr I,Hussain I,Waseem M,Arif M

更新日期：2017-10-01 00:00:00

abstract：:The helix-loop-helix (HLH) family of transcriptional factors is a key player in a wide range of developmental processes in organisms from mammals to microbes. We previously identified the bHLH transcription factor SclR in Aspergillus oryzae and found that the loss of SclR function led to significant phenotypic changes...

journal_title：Applied microbiology and biotechnology

authors： Jin FJ,Han P,Zhuang M,Zhang ZM,Jin L,Koyama Y

更新日期：2018-01-01 00:00:00

abstract：:A large percentage of organic fuel consumed in a microbial fuel cell (MFC) is lost as a result of oxygen transfer through the cathode. In order to understand how this oxygen transfer affects the microbial community structure, reactors were operated in duplicate using three configurations: closed circuit (CC; with curr...

journal_title：Applied microbiology and biotechnology

authors： Shehab N,Li D,Amy GL,Logan BE,Saikaly PE

更新日期：2013-11-01 00:00:00

abstract：:This study demonstrated for the first time the possibility to remove and partially recover the Ruthenium contained in industrial effluents by using purple non sulfur bacteria (PNSB) as microbial biosorbents. Up to date, the biosorption was only claimed as possible tool for the removal of the platinum-group metals (PGM...

journal_title：Applied microbiology and biotechnology

authors： Colica G,Caparrotta S,De Philippis R

更新日期：2012-07-01 00:00:00

abstract：:Streptococcus mutans is the main cause of dental caries. We evaluated the therapeutic effect of variable regions of a llama heavy chain antibody fragments directed against S. mutans named S36-VHH (S for Streptococcus) alone or fused with glucose oxidase (GOx) from Aspergillus niger. Western blot analysis and ELISA rev...

journal_title：Applied microbiology and biotechnology

authors： Krüger C,Hultberg A,Marcotte H,Hermans P,Bezemer S,Frenken LG,Hammarström L

更新日期：2006-10-01 00:00:00

abstract：:Serratia marcescens has been proved to be a potential strain for industrial 2,3-butanediol production for its high yield, productivity, and other advantages. In this study, the genes slaA, slaB, slaC, and slaR were successfully cloned which were further confirmed to be encoding acetolactate decarboxylase, acetolactate...

journal_title：Applied microbiology and biotechnology

authors： Rao B,Zhang LY,Sun J,Su G,Wei D,Chu J,Zhu J,Shen Y

更新日期：2012-03-01 00:00:00

abstract：:CYP102A1 is an efficient medium- to long-chain fatty acid hydroxylase that is able to accept a wide range of non-natural substrates which bear no resemblance to the natural ones. 4-Hexylbenzoic acid (HBA) and 4-nonyloxybenzoic acid (NOBA) were identified as CYP102A1 substrates via screening studies using the BD Oxygen...

journal_title：Applied microbiology and biotechnology

authors： Gudiminchi RK,Smit MS

更新日期：2011-04-01 00:00:00

abstract：:This study was aimed to investigate the effect of a single-chain fragment variable antibody of connective tissue growth factor (anti-CTGF scFv) against the differentiation of fibroblast into myofibroblast. The scFv antibody was firstly expressed in Escherichia coli cells and was then purified by affinity chromatograph...

journal_title：Applied microbiology and biotechnology

authors： Wu G,Wang X,Deng X,Wu P,Xue X,Yan X,Wang X

更新日期：2012-03-01 00:00:00

abstract：:Clostridium tyrobutyricum is a promising organism for butyrate and n-butanol production, but cannot grow on sucrose. Three genes (scrA, scrB, and scrK) involved in the sucrose catabolic pathway, along with an aldehyde/alcohol dehydrogenase gene, were cloned from Clostridium acetobutylicum and introduced into C. tyrobu...

journal_title：Applied microbiology and biotechnology

authors： Zhang J,Yu L,Xu M,Yang ST,Yan Q,Lin M,Tang IC

更新日期：2017-05-01 00:00:00

abstract：:Hemidesmus indicus (L.) R. Br. ex Schult., commonly known as Indian sarsaparilla or Anantamul, is an ethnopharmacologically important medicinal plant from the family Apocynaceae. Biosynthesis of an array of bioactive compounds such as methoxybenzaldehydes and their derivatives has been accountable for its intensive me...

journal_title：Applied microbiology and biotechnology

authors： Nandy S,Singh J,Pandey DK,Dey A

更新日期：2020-10-01 00:00:00

abstract：:The Vitreoscilla hemoglobin (VHb) gene (vgb) was integrated into the chromosome of Bacillus thuringiensis BMB171 using integrative vector pEG491. The production of VHb was confirmed by CO-difference spectra analysis. Fermentation experiments results showed that with the production of VHb, the critical oxygen concentra...

journal_title：Applied microbiology and biotechnology

authors： Liang F,Shouwen C,Ming S,Ziniu Y

更新日期：2007-02-01 00:00:00

abstract：:Cryparin is an abundant cell-wall-associated hydrophobin of Cryphonectria parasitica. Although cryparin is encoded as a single copy gene, it is the most abundant protein produced by this fungus when grown in liquid culture. Studies to characterize the transcriptional regulatory element(s) found that the fragment betwe...

journal_title：Applied microbiology and biotechnology

authors： Kwon BR,Kim MJ,Park JA,Chung HJ,Kim JM,Park SM,Yun SH,Yang MS,Kim DH

更新日期：2009-05-01 00:00:00

abstract：:Escherichia coli-the powerhouse for recombinant protein production-is rapidly gaining status as a reliable and efficient host for secretory expression. An improved understanding of protein translocation processes and its mechanisms has inspired and accelerated the development of new tools and applications in this fiel...

journal_title：Applied microbiology and biotechnology

authors： Low KO,Muhammad Mahadi N,Md Illias R

更新日期：2013-05-01 00:00:00

abstract：:It was found that benzothiazole, 2-oxybenzothiazole and 2-benzothiazolesulphonate were degraded in activated sludge systems. 2-Mercaptobenzothiazole (MBT) was more resistant, although the first step in MBT degradation seemed to be transformation to the sulphonate form. At higher MBT concentrations, it was transformed ...

journal_title：Applied microbiology and biotechnology

authors： De Vos D,De Wever H,Verachtert H

更新日期：1993-07-01 00:00:00

abstract：:Sulfation is an important way for detoxifying xenobiotics and endobiotics including catechols. Enzymatic sulfation occurs usually with high chemo- and/or regioselectivity under mild reaction conditions. In this study, a two-step p-NPS-4-AAP screening system for laboratory evolution of aryl sulfotransferase B (ASTB) wa...

journal_title：Applied microbiology and biotechnology

authors： Ji Y,Islam S,Mertens AM,Sauer DF,Dhoke GV,Jakob F,Schwaneberg U

更新日期：2019-05-01 00:00:00

abstract：:A widespread and hitherto by far underexploited potential among ecologically diverse fungi to pretreat wheat straw and digestate from maize silage in the future perspective of using such lignocellulosic feedstock for fermentative bioenergy production was inferred from a screening of nine freshwater ascomycetes, 76 iso...

journal_title：Applied microbiology and biotechnology

authors： Singh S,Harms H,Schlosser D

更新日期：2014-04-01 00:00:00

abstract：:Oleaginous yeasts (18 strains) were grown in ethanol media under various cultivation conditions (33 biomass samples). It was found that lipid and lipid-free fractions of dry biomass have elemental composition and biomass reductivity very close to values which can be considered as biological constants. The energy conte...

journal_title：Applied microbiology and biotechnology

authors： Minkevich IG,Dedyukhina EG,Chistyakova TI

更新日期：2010-10-01 00:00:00

abstract：:This study has investigated the acclimation of ammonia-oxidizing communities (AOC) to low dissolved oxygen (DO) concentrations. Under controlled laboratory conditions, two sequencing batch reactors seeded with activated sludge from the same source were operated at high DO (near saturation) and low DO (0.1 mg O₂/L) con...

journal_title：Applied microbiology and biotechnology

authors： Arnaldos M,Kunkel SA,Stark BC,Pagilla KR

更新日期：2013-12-01 00:00:00

abstract：:Crude peroxidase preparations from the lignocellulose-degrading actinomycete, Streptomyces viridosporus T7A, were shown to decolorize several azo dye isomers and showed a correlation of dye structure to degradability similar to that shown by fungal Mn-peroxidase, an enzyme not previously described in actinomycetes. Ad...

journal_title：Applied microbiology and biotechnology

authors： Burke NS,Crawford DL

更新日期：1998-05-01 00:00:00