Description of a cellulose-binding domain and a linker sequence from Aspergillus fungi.

abstract：:Microalgal starch is a potential feedstock for biofuel production. Nutrient stress is widely used to stimulate starch accumulation in microalgae. Cell growth and starch accumulation in the marine green microalga Tetraselmis subcordiformis were evaluated under extracellular phosphorus deprivation with initial cell dens...

journal_title：Applied microbiology and biotechnology

authors： Yao CH,Ai JN,Cao XP,Xue S

更新日期：2013-07-01 00:00:00

abstract：:Edwardsiella tarda is a leading fish pathogen haunting worldwide aquaculture industry. In E. tarda, two-component system EsrA-EsrB positively regulates type III and VI secretion systems (T3SS and T6SS) and negatively regulates hemolysin EthA, which has been demonstrated to be essential for the invasion processes in fi...

journal_title：Applied microbiology and biotechnology

authors： Yang W,Wang L,Zhang L,Qu J,Wang Q,Zhang Y

更新日期：2015-02-01 00:00:00

abstract：:Immunostimulatory glucose polymers known as beta-glucans have been studied for many years. Our laboratory has prepared and characterized a novel microparticulate beta-glucan (MG) from the budding yeast Saccharomyces cerevisiae. Because MG particles are rapidly phagocytized by murine peritoneal macrophages and induce t...

journal_title：Applied microbiology and biotechnology

authors： Berner VK,Sura ME,Hunter KW Jr

更新日期：2008-10-01 00:00:00

abstract：:High vanillin productivity was achieved in the batch biotransformation of ferulic acid by Streptomyces sp. strain V-1. Due to the toxicity of vanillin and the product inhibition, fed-batch biotransformation with high concentration of ferulic acid was unsuccessful. To solve this problem and improve the vanillin yield, ...

journal_title：Applied microbiology and biotechnology

authors： Hua D,Ma C,Song L,Lin S,Zhang Z,Deng Z,Xu P

更新日期：2007-03-01 00:00:00

abstract：:To seek more information on function of two-component regulatory systems (TCSs) in Streptomyces coelicolor, a dozen TCS-knockout mutants were generated, and phenotype changes were determined. One TCS (SCO5403/5404)-deleted mutant with phenotype change was obtained. Here, we report the characterization of this novel TC...

journal_title：Applied microbiology and biotechnology

authors： Lu Y,Wang W,Shu D,Zhang W,Chen L,Qin Z,Yang S,Jiang W

更新日期：2007-12-01 00:00:00

abstract：:Endophytic actinobacteria, which exist in the inner tissues of living plants, have attracted increasing attention among taxonomists, ecologists, agronomists, chemists and evolutionary biologists. Numerous studies have indicated that these prolific actinobacteria appear to have a capacity to produce an impressive array...

journal_title：Applied microbiology and biotechnology

authors： Qin S,Xing K,Jiang JH,Xu LH,Li WJ

更新日期：2011-02-01 00:00:00

abstract：:The araA gene encoding an L-arabinose isomerase (L-AI) from the acido-thermophilic bacterium Acidothermus cellulolytics ATCC 43068 was cloned and overexpressed in Escherichia coli. The open reading frame of the L-AI consisted of 1,503 nucleotides encoding 501 amino acid residues. The recombinant L-AI was purified to h...

journal_title：Applied microbiology and biotechnology

authors： Cheng L,Mu W,Zhang T,Jiang B

更新日期：2010-04-01 00:00:00

abstract：:A reactive iron barrier in a contaminated aquifer with low pH was found to dechlorinate 1,2-dichloroethane (1,2-DCA) in situ. This chlorinated ethane is known to resist abiotic reduction by zero valent iron. Samples taken up-gradient and within the barrier were used to inoculate anaerobic batch cultures amended with v...

journal_title：Applied microbiology and biotechnology

authors： Zemb O,Lee M,Low A,Manefield M

更新日期：2010-09-01 00:00:00

abstract：:Microbial sulfate reduction with acetate as carbon source and electron donor was investigated at salinity levels between 0.53 and 1.48%. The experiment was carried out in a 2.3-1 upflow anaerobic sludge blanket reactor inoculated with granular methanogenic sludge. A pH of 8.3, a temperature of 32 +/- 1 degrees C and a...

journal_title：Applied microbiology and biotechnology

authors： Muthumbi W,Boon N,Boterdaele R,De Vreese I,Top EM,Verstraete W

更新日期：2001-06-01 00:00:00

abstract：:A recent study of the UV-generated glucose-utilizing mutant Ralstonia eutropha G⁺1 comprising transcriptomic and proteomic analyses revealed clear evidence that glucose is transported by the N-acetylglucosamine-specific phosphotransferase system (PTS(Nag)), which is overexpressed in this mutant due to a derepression o...

journal_title：Applied microbiology and biotechnology

authors： Raberg M,Kaddor C,Kusian B,Stahlhut G,Budinova R,Kolev N,Bowien B,Steinbüchel A

更新日期：2012-08-01 00:00:00

abstract：:The extremely thermophilic anaerobic archaeon strain, HJ21, was isolated from a deep-sea hydrothermal vent, could produce hyperthermophilic alpha-amylase, and later was identified as Thermococcus from morphological, biochemical, and physiological characteristics and the 16S ribosomal RNA gene sequence. The extracellul...

journal_title：Applied microbiology and biotechnology

authors： Wang S,Lu Z,Lu M,Qin S,Liu H,Deng X,Lin Q,Chen J

更新日期：2008-09-01 00:00:00

abstract：:Spillage of fossil-based oils during their conveyance through water conduits are sporadic, but significant environmental disasters. As the viscous hydrocarbons of the crude oils spread on water surface and choke aquatic life to death, their effective degradation is crucial for ecological balance. Though chemical and m...

journal_title：Applied microbiology and biotechnology

authors： Patel S,Homaei A,Patil S,Daverey A

更新日期：2019-01-01 00:00:00

abstract：:Due to variations in the production levels, a full-scale sequencing batch reactor (SBR) for post-treatment of tannery wastewater was exposed to low and high ammonia load periods. In order to study how these changes affected the N-removal capacity, the microbiology of the reactor was studied by a diverse set of techniq...

journal_title：Applied microbiology and biotechnology

authors： Cabezas A,Draper P,Etchebehere C

更新日期：2009-10-01 00:00:00

abstract：:We have fused the epidermal growth factor (EGF) to the amino terminus of Pseudomonas exotoxin A (PE) to create a cytotoxic agent, designated EGF-PE, which preferentially kills EGF-receptor-bearing cells. In this study, we analyzed the effect of the Ia domain, the binding domain of PE on the cytotoxicity of EGF-PE towa...

journal_title：Applied microbiology and biotechnology

authors： Liao CW,Hseu TH,Hwang J

更新日期：1995-07-01 00:00:00

abstract：:Bacteria that grow on p-xylene, p-toluic acid, and terephthalic acid (TPA) were isolated from a wastewater bioreactor that is used to treat a waste stream that contains all three of these compounds. Although previously described aerobic bacteria degrade p-xylene by initially oxidizing a single methyl group to form p-t...

journal_title：Applied microbiology and biotechnology

authors： Bramucci MG,McCutchen CM,Singh M,Thomas SM,Larsen BS,Buckholz J,Nagarajan V

更新日期：2002-02-01 00:00:00

abstract：:The addition of humic acid (HA) to polycyclic aromatic hydrocarbon (PAH) contaminated systems has been shown to enhance, inhibit, or have no effect on the biodegradation of these PAHs. In this study, the surfactant effects of Elliott soil HA (ESHA) at two pH values were tested. At pH 7.0, ESHA did not behave as a surf...

journal_title：Applied microbiology and biotechnology

authors： Liang Y,Britt DW,McLean JE,Sorensen DL,Sims RC

更新日期：2007-04-01 00:00:00

abstract：:We used experimental evolution in order to identify genes involved in the adaptation of Saccharomyces cerevisiae to the early stages of alcoholic fermentation. Evolution experiments were run for about 200 generations, in continuous culture conditions emulating the initial stages of wine fermentation. We performed whol...

journal_title：Applied microbiology and biotechnology

authors： Mangado A,Tronchoni J,Morales P,Novo M,Quirós M,Gonzalez R

更新日期：2015-02-01 00:00:00

abstract：:In Escherichia coli, the enzyme called cysteine desulfhydrase (CD), which is responsible for L-cysteine degradation, was investigated by native-PAGE and CD activity staining of crude cell extracts. Analyses with gene-disrupted mutants showed that CD activity resulted from two enzymes: tryptophanase (TNase) encoded by ...

journal_title：Applied microbiology and biotechnology

authors： Awano N,Wada M,Kohdoh A,Oikawa T,Takagi H,Nakamori S

更新日期：2003-08-01 00:00:00

abstract：:Nanosized elemental sulfur (ES) is already reported to exert superior antimicrobial efficacy than micron-sized ES, which encourages their use in drugs and therapeutics. The aim of the present study is to explore the possible route and mode of antimicrobial action of orthorhombic (α-SNPs) and monoclinic (β-SNPs) allotr...

journal_title：Applied microbiology and biotechnology

authors： Roy Choudhury S,Mandal A,Ghosh M,Basu S,Chakravorty D,Goswami A

更新日期：2013-07-01 00:00:00

abstract：:Myogenesis involves the determination of progenitor cells to myoblasts, their fusion to yield multinuclear myotubes, and the maturation of myotubes to muscle fibres. This development is reflected in a time pattern of gene expression, e.g. of genes coding for desmin, the myogenic factors myogenin and myoD, the acetylch...

journal_title：Applied microbiology and biotechnology

authors： Bardouille C,Lehmann J,Heimann P,Jockusch H

更新日期：2001-05-01 00:00:00

abstract：:Carboligations catalyzed by aldolases or thiamine diphosphate (ThDP)-dependent enzymes are well-known in biocatalysis to deliver enantioselective chain elongation reactions. A pyruvate-dependent aldolase (2-oxo-3-deoxy-6-phosphogluconate aldolase [EDA]) introduces a chiral center when reacting with the electrophile, g...

journal_title：Applied microbiology and biotechnology

authors： Schapfl M,Baier S,Fries A,Ferlaino S,Waltzer S,Müller M,Sprenger GA

更新日期：2018-10-01 00:00:00

abstract：:Microbial cell factories offer an economic approach for synthesizing "natural'" aromatic flavor compounds. During their fermentation process, the inefficient synthesis pathway and product cytotoxicity are the major barriers to the high-level production. This study combined metabolic engineering and tolerance engineeri...

journal_title：Applied microbiology and biotechnology

authors： Wang Y,Zhang Z,Lu X,Zong H,Zhuge B

更新日期：2020-12-01 00:00:00

abstract：:In this study, a high yield production bioprocess with recombinant Bacillus megaterium for the production of the extracellular enzyme levansucrase (SacB) was developed. For basic optimization of culture parameters and nutrients, a recombinant B. megaterium reporter strain that produced green fluorescent protein under ...

journal_title：Applied microbiology and biotechnology

authors： Korneli C,Biedendieck R,David F,Jahn D,Wittmann C

更新日期：2013-04-01 00:00:00

abstract：:In the Candida antarctica lipase B-catalyzed hydrolysis of (R,S)-azolides derived from (R,S)-N-protected proline in water-saturated methyl tert-butyl ether (MTBE), high enzyme activity with excellent enantioselectivity (V (S) V (R) ...

journal_title：Applied microbiology and biotechnology

authors： Wu CH,Pen CW,Wang PY,Tsai SW

更新日期：2013-02-01 00:00:00

abstract：:Biofilm development in methanogenic fluidized-bed reactors with sand as the carrier was studied on a laboratory scale. The microorganisms present in consecutive layers of the biofilm of mature sludge granules were preliminarily characterized on the basis of their morphology, element composition and adhesion capacity a...

journal_title：Applied microbiology and biotechnology

authors： Lauwers AM,Heinen W,Gorris LG,van der Drift C

更新日期：1990-06-01 00:00:00

abstract：:For the biotechnological production of L: -lysine, mainly strains of Corynebacterium glutamicum are used, which have been obtained by classical mutagenesis and screening or selection or by metabolic engineering. Gene targets for the amplification and deregulation of the lysine biosynthesis pathway, for the improvement...

journal_title：Applied microbiology and biotechnology

authors： Sindelar G,Wendisch VF

更新日期：2007-09-01 00:00:00

abstract：:The ω-3 fatty acid desaturase (ω3Des) is a key enzyme in the biosynthesis of polyunsaturated fatty acids (PUFAs). However, the enzyme exhibits a significant preference towards different fatty acid substrates. To examine the molecular mechanism of its substrate specificity, a series of site-directed mutants were constr...

journal_title：Applied microbiology and biotechnology

authors： Rong C,Chen H,Wang M,Gu Z,Zhao J,Zhang H,Chen W,Chen YQ

更新日期：2018-11-01 00:00:00

abstract：:We have recently reported that expression of an unidentified heme protein is enhanced in a nitrifying activated sludge community under low (0.1 mg O2/L) dissolved oxygen (DO) conditions. A preliminary assessment suggested it may be a type of hemoglobin (Hb) or a lesser-known component of the energy-transducing pathway...

journal_title：Applied microbiology and biotechnology

authors： Arnaldos M,Kunkel SA,Stark BC,Pagilla KR

更新日期：2014-04-01 00:00:00

abstract：:A general procedure for the high yield immobilization of enzymes with the help of specific anti-enzyme antibodies is described. Polyclonal antibodies were raised against Aspergillus niger glucose oxidase and horseradish peroxidase in rabbits and the gamma globulin (IgG) fraction from the immune sera isolated by ammoni...

journal_title：Applied microbiology and biotechnology

authors： Farooqi M,Sosnitza P,Saleemuddin M,Ulber R,Scheper T

更新日期：1999-09-01 00:00:00

abstract：:The demand of renewable energy sources, i.e. biomass, is steadily increasing worldwide to reduce the need of fossil energy sources. Biomass such as energy crops, woody species, forestry and agricultural residues are the most common renewable energy sources. Due to uneven demand for wood fuel, the material is mostly st...

journal_title：Applied microbiology and biotechnology

authors： Noll M,Jirjis R

更新日期：2012-08-01 00:00:00