Improving lysine production by Corynebacterium glutamicum through DNA microarray-based identification of novel target genes.

Abstract:

:For the biotechnological production of L: -lysine, mainly strains of Corynebacterium glutamicum are used, which have been obtained by classical mutagenesis and screening or selection or by metabolic engineering. Gene targets for the amplification and deregulation of the lysine biosynthesis pathway, for the improvement of carbon precursor supply and of nicotinamide adenine dinucleotide phosphate (reduced form) (NADPH) regeneration, are known. To identify novel target genes to improve lysine production, the transcriptomes of the classically obtained lysine producing strain MH20-22B and several other C. glutamicum strains were compared. As lysine production by the classically obtained strain, which possesses feedback-resistant aspartokinase and is leucine auxotrophic, exceeds that of a genetically defined leucine auxotrophic wild-type derivative possessing feedback-resistant aspartokinase, additional traits beneficial for lysine production are present. NCgl0855, putatively encoding a methyltransferase, and the amtA-ocd-soxA operon, encoding an ammonium uptake system, a putative ornithine cyclodeaminase and an uncharacterized enzyme, were among the genes showing increased expression in the classically obtained strain irrespective of the presence of feedback-resistant aspartokinase. Lysine production could be improved by about 40% through overexpression of NCgl0855 or the amtA-ocd-soxA operon. Thus, novel target genes for the improvement of lysine production could be identified in a discovery-driven approach based on global gene expression analysis.

authors

Sindelar G,Wendisch VF

doi

10.1007/s00253-007-0916-x

subject

Has Abstract

pub_date

2007-09-01 00:00:00

pages

677-89

issue

3

eissn

0175-7598

issn

1432-0614

journal_volume

76

pub_type

杂志文章
  • Ammonia manipulates the ammonia-oxidizing archaea and bacteria in the coastal sediment-water microcosms.

    abstract::Ammonia was observed as a potential significant factor to manipulate the abundance and activity of ammonia-oxidizing microorganisms (AOMs) in water environments. For the first time, this study confirmed this phenomenon by laboratory cultivation. In a series of estuarine sediment-coastal water microcosms, we investigat...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-015-6524-2

    authors: Zhang Y,Chen L,Dai T,Sun R,Wen D

    更新日期:2015-08-01 00:00:00

  • Bacterial response to acetate challenge: a comparison of tolerance among species.

    abstract::Although acetate formation and tolerance are important criteria for various aspects of biotechnological process development, available studies on acetate tolerance in different species are disparate. We evaluate the response of eight bacterial strains, including two variants of Escherichia coli, two variants of Staphy...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s002530000339

    authors: Lasko DR,Zamboni N,Sauer U

    更新日期:2000-08-01 00:00:00

  • Penicillin G acylase from Achromobacter sp. CCM 4824 : an efficient biocatalyst for syntheses of beta-lactam antibiotics under conditions employed in large-scale processes.

    abstract::Penicillin G acylase from Achromobacter sp. (NPGA) was studied in the enzymatic synthesis of β-lactam antibiotics by kinetically controlled N-acylation. When compared with penicillin acylase of Escherichia coli (PGA), the NPGA was significantly more efficient at syntheses of ampicillin and amoxicillin (higher S/H rati...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-013-4945-3

    authors: Bečka S,Štěpánek V,Vyasarayani RW,Grulich M,Maršálek J,Plháčková K,Dobišová M,Marešová H,Plačková M,Valešová R,Palyzová A,Datla A,Ashar TK,Kyslík P

    更新日期:2014-02-01 00:00:00

  • Production of trehalose synthase from a basidiomycete, Grifola frondosa, in Escherichia coli.

    abstract::The genomic DNA and cDNA for a gene encoding a novel trehalose synthase (TSase) catalyzing trehalose synthesis from alpha-D-glucose 1-phosphate and D-glucose were cloned from a basidiomycete, Grifola frondosa. Nucleotide sequencing showed that the 732-amino-acid TSase-encoding region was separated by eight introns. Co...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s002530051276

    authors: Saito K,Yamazaki H,Ohnishi Y,Fujimoto S,Takahashi E,Horinouchi S

    更新日期:1998-08-01 00:00:00

  • Purification of alkaline proteases from a Bacillus strain and their possible interrelationship.

    abstract::Alkalophilic Bacillus sp. KSM-K16 produced three alkaline proteases, as detected by polyacrylamide gel electrophoresis (PAGE). The major protease, designated M protease, was recently purified to homogeneity and its properties were characterized. In the present study, two minor proteases, designated H protease and N pr...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s002530050650

    authors: Kobayashi T,Hakamada Y,Hitomi J,Koike K,Ito S

    更新日期:1996-03-01 00:00:00

  • Engineering Escherichia coli for efficient cellobiose utilization.

    abstract::Escherichia coli normally cannot utilize the β-glucoside sugar cellobiose as a carbon and energy source unless a stringent selection pressure for survival is present. The cellobiose-utilization phenotype can be conferred by mutations in the two cryptic operons, chb and asc. In this study, the cellobiose-utilization ph...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-011-3434-9

    authors: Vinuselvi P,Lee SK

    更新日期:2011-10-01 00:00:00

  • Phosphate starvation controls lactose metabolism to produce recombinant protein in Escherichia coli.

    abstract::Phosphate is one of the major constituents in growth media. It closely regulates central carbon and energy metabolism. Biochemical reactions in central carbon metabolism are known to be regulated by phosphorylation and dephosphorylation of enzymes. Phosphate scarcity can limit microbial productivity. However, microorg...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-020-10935-y

    authors: Pandi K,Chauhan AS,Khan WH,Rathore AS

    更新日期:2020-11-01 00:00:00

  • Cell surface-engineered yeast displaying a histidine oligopeptide (hexa-His) has enhanced adsorption of and tolerance to heavy metal ions.

    abstract::A histidine oligopeptide (hexa-His) with the ability to chelate divalent heavy metal ions was displayed on the yeast cell surface for the purpose of enhanced adsorption of heavy metal ions. We genetically fused a hexa-His-encoding gene with the gene encoding the C-terminal half of alpha-agglutinin that includes a glyc...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s002530100813

    authors: Kuroda K,Shibasaki S,Ueda M,Tanaka A

    更新日期:2001-12-01 00:00:00

  • Biocatalytic production of Ɛ-caprolactone using Geotrichum candidum cells immobilized on functionalized silica.

    abstract::Immobilization of the Geotrichum candidum (CCT 1205) cell with functionalized silica creates promising biocatalysts for production of ɛ-caprolactone. The results obtained by immobilization of the whole cell on SiO2-NH2 and SiO2-SH supports indicate that the presence of reactive functional groups on the support may pro...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-020-10875-7

    authors: Silva ALP,da Silva Caridade TN,Magalhães RR,de Sousa KT,de Sousa CC,Vale JA

    更新日期:2020-10-01 00:00:00

  • Biochemical characterization and low-resolution SAXS shape of a novel GH11 exo-1,4-β-xylanase identified in a microbial consortium.

    abstract::Biotechnologies that aim to produce renewable fuels, chemicals, and bioproducts from residual ligno(hemi)cellulosic biomass mostly rely on enzymatic depolymerization of plant cell walls (PCW). This process requires an arsenal of diverse enzymes, including xylanases, which synergistically act on the hemicellulose, redu...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-019-10033-8

    authors: Evangelista DE,de Oliveira Arnoldi Pellegrini V,Santo ME,McQueen-Mason S,Bruce NC,Polikarpov I

    更新日期:2019-10-01 00:00:00

  • Stereospecific microbial production of isoflavanones from isoflavones and isoflavone glucosides.

    abstract::A Gram-negative anaerobic microorganism, MRG-1, isolated from human intestine showed high activities of deglycosylation and reduction of daidzin, based on rapid TLC analysis. A rod-shaped strain MRG-1 was identified as a new species showing 91.0% homology to Coprobacillus species, based on 16S rRNA sequence analysis. ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-011-3310-7

    authors: Park HY,Kim M,Han J

    更新日期:2011-08-01 00:00:00

  • Identification of Thermotoga maritima MSB8 GH57 α-amylase AmyC as a glycogen-branching enzyme with high hydrolytic activity.

    abstract::AmyC, a glycoside hydrolase family 57 (GH57) enzyme of Thermotoga maritima MSB8, has previously been identified as an intracellular α-amylase playing a role in either maltodextrin utilization or storage polysaccharide metabolism. However, the α-amylase specificity of AmyC is questionable as extensive phylogenetic anal...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-019-09938-1

    authors: Zhang X,Leemhuis H,Janeček Š,Martinovičová M,Pijning T,van der Maarel MJEC

    更新日期:2019-08-01 00:00:00

  • Microbial aldolases as C-C bonding enzymes--unknown treasures and new developments.

    abstract::Aldolases are a specific group of lyases that catalyze the reversible stereoselective addition of a donor compound (nucleophile) onto an acceptor compound (electrophile). Whereas most aldolases are specific for their donor compound in the aldolization reaction, they often tolerate a wide range of aldehydes as acceptor...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-006-0422-6

    authors: Samland AK,Sprenger GA

    更新日期:2006-07-01 00:00:00

  • Effects of oxygen supply on the production of nikkomycin with immobilized cells of Streptomyces tendae.

    abstract::For continuous production of the antibiotic nikkomycin immobilized cells have been used in a fluidized bed bioreactor. Cells of Streptomyces tendae were immobilized on sintered glass particles. Different biomass concentrations on the particles correspond to different thicknesses of mycelial layers because growth occur...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00170913

    authors: Trück HU,Chmiel H,Hammes WP,Trösch W

    更新日期:1990-10-01 00:00:00

  • Characterization of T-DNA insertion mutants with decreased virulence in the entomopathogenic fungus Beauveria bassiana JEF-007.

    abstract::The bean bug, Riptortus pedestris, is a major agricultural pest that reduces crop quality and value. Chemical pesticides have contributed to pest management, but resistance to these chemicals has significantly limited their use. Alternative strategies with different modes of action, such as entomopathogenic fungi, are...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-016-7734-y

    authors: Kim S,Lee SJ,Nai YS,Yu JS,Lee MR,Yang YT,Kim JS

    更新日期:2016-10-01 00:00:00

  • A promiscuous prenyltransferase from Aspergillus oryzae catalyses C-prenylations of hydroxynaphthalenes in the presence of different prenyl donors.

    abstract::Prenyltransferases of the dimethylallyltryptophan synthase (DMATS) superfamily are involved in the biosynthesis of secondary metabolites and show broad substrate specificity towards their aromatic substrates with a high regioselectivity for the prenylation reactions. Most members of this superfamily accepted as prenyl...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-014-5509-x

    authors: Pockrandt D,Sack C,Kosiol T,Li SM

    更新日期:2014-06-01 00:00:00

  • Soil bacterial community responses to revegetation of moving sand dune in semi-arid grassland.

    abstract::Grasslands in semi-arid Northern China are widely desertified, thus inducing the formation of a large area of moving sand lands. Revegetation of the sandy land is commonly adopted to restore degraded grasslands. The structure of the soil microbial community might dramatically change during degradation and recovery bec...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-017-8336-z

    authors: Cao C,Zhang Y,Cui Z,Feng S,Wang T,Ren Q

    更新日期:2017-08-01 00:00:00

  • Monitoring survival and gene transfer in soil microcosms of recombinant Escherichia coli designed to represent an industrial production strain.

    abstract::A genetically engineered microorganism (GEM) was designed to exemplify bacterial strains used for the production of biological material in industry. The recombinant DNA was located on a safety plasmid (pUC19). Survival and persistence of the GEM and its recombinant DNA (rDNA) was determined in soil microcosms by using...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00184696

    authors: Henschke RB,Henschke EJ,Schmidt FR

    更新日期:1991-05-01 00:00:00

  • Biosynthesis of bioactive O-methylated flavonoids in Escherichia coli.

    abstract::Two bioactive O-methylflavonoids, sakuranetin (7-O-methylnaringenin) and ponciretin (7-O-methylnaringenin), were synthesized in Escherichia coli. Sakuranetin inhibits germination of Magnaporthe grisea, and ponciretin is a potential inhibitor of Helicobacter pylori. To achieve this, we reconstructed the naringenin bios...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-013-5020-9

    authors: Kim MJ,Kim BG,Ahn JH

    更新日期:2013-08-01 00:00:00

  • A novel heterotrophic nitrifying and aerobic denitrifying bacterium, Zobellella taiwanensis DN-7, can remove high-strength ammonium.

    abstract::A novel heterotrophic bacterium capable of heterotrophic nitrification and aerobic denitrification was isolated from ammonium contaminated landfill leachate and physiochemical and phylogenetically identified as Zobellella taiwanensis DN-7. DN-7 converted nitrate, nitrate, and ammonium to N2 as the primary end product....

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-016-7290-5

    authors: Lei Y,Wang Y,Liu H,Xi C,Song L

    更新日期:2016-05-01 00:00:00

  • Development of a biosensing platform based on a laccase-hydrophobin chimera.

    abstract::A simple and stable immobilization of a laccase from Pleurotus ostreatus was obtained through genetic fusion with a self-assembling and adhesive class I hydrophobin. The chimera protein was expressed in Pichia pastoris and secreted into the culture medium. The crude culture supernatant was directly used for coatings o...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-019-09678-2

    authors: Sorrentino I,Giardina P,Piscitelli A

    更新日期:2019-04-01 00:00:00

  • Diversity, abundance, and spatial distribution of riverine microbial communities response to effluents from swine farm versus farmhouse restaurant.

    abstract::The 454 pyrosequencing technique was applied to evaluate microbial community composition in sediment and water samples collected from the river receiving effluents from a swine farm and a farmhouse restaurant, respectively. For each sample, 4,600 effective sequences were selected and used to do the bacterial diversity...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-014-5772-x

    authors: Lu XM,Lu PZ

    更新日期:2014-09-01 00:00:00

  • Soil microbial community responses to antibiotic-contaminated manure under different soil moisture regimes.

    abstract::Sulfadiazine (SDZ) is an antibiotic frequently administered to livestock, and it alters microbial communities when entering soils with animal manure, but understanding the interactions of these effects to the prevailing climatic regime has eluded researchers. A climatic factor that strongly controls microbial activity...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-014-5717-4

    authors: Reichel R,Radl V,Rosendahl I,Albert A,Amelung W,Schloter M,Thiele-Bruhn S

    更新日期:2014-01-01 00:00:00

  • Metabolism of fluoranthene by Mycobacterium sp. strain AP1.

    abstract::The pyrene-degrading Mycobacterium strain AP1 was found to utilize fluoranthene as a sole source of carbon and energy. Identification of metabolites formed from fluoranthene (by growing cells and washed-cell suspensions), the kinetics of metabolite accumulation, and metabolite-feeding studies all indicated that strain...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-005-0120-9

    authors: López Z,Vila J,Minguillón C,Grifoll M

    更新日期:2006-05-01 00:00:00

  • Biology, dynamics, and applications of transposable elements in basidiomycete fungi.

    abstract::The phylum Basidiomycota includes filamentous fungi and yeast species with different ecological and genomic characteristics. Transposable elements (TEs) are abundant components of most eukaryotic genomes, and their transition from being genomic parasites to key drivers of genomic architecture, functionality, and evolu...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-017-8097-8

    authors: Castanera R,Borgognone A,Pisabarro AG,Ramírez L

    更新日期:2017-02-01 00:00:00

  • Functions and impact of tal-like genes in animals with regard to applied aspects.

    abstract::A large number of DNAs in eukaryote genomes can code for atypical transcripts, and their functions are controversial. It has been reported that the transcripts contain many small open reading frames (sORFs), which were originally considered as non-translatable RNAs. However, increasing evidence has suggested that some...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-018-9159-2

    authors: Zhu M,Hu X,Cao G,Xue R,Gong C

    更新日期:2018-08-01 00:00:00

  • Astaxanthin production by Phaffia rhodozyma and Haematococcus pluvialis: a comparative study.

    abstract::Phaffia rhodozyma (now Xanthophyllomyces dendrorhous) and Haematococcus pluvialis are known as the major prominent microorganisms able to synthesize astaxanthin natural pigment. Important research efforts have been made to determine optimal conditions for astaxanthin synthesis. When the focus is on astaxanthin product...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-007-0889-9

    authors: Domínguez-Bocanegra AR,Ponce-Noyola T,Torres-Muñoz JA

    更新日期:2007-06-01 00:00:00

  • Identification and role analysis of an intermediate produced by a polygenic mutant of Monascus pigments cluster in Monascus ruber M7.

    abstract::Monascus pigments (Mps) are a group of azaphilonic secondary metabolites produced by Monascus spp. via a polyketide pathway. A mutant deleted an about 30 kb region of Mps gene cluster from Monascus ruber M7 was isolated previously, which produces a high amount of a light yellow pigment. The current study revealed that...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-016-7397-8

    authors: Liu J,Zhou Y,Yi T,Zhao M,Xie N,Lei M,Liu Q,Shao Y,Chen F

    更新日期:2016-08-01 00:00:00

  • Cardoon-based rennets for cheese production.

    abstract::The use of crude aqueous extracts of Cynara cardunculus flowers as coagulants in the production of high-quality sheep and goat cheeses-as are the cases of several Portuguese and Spanish cheese varieties with Protected Designation of Origin status-has been maintained since ancient times. The unique rheological attribut...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-018-9032-3

    authors: Almeida CM,Simões I

    更新日期:2018-06-01 00:00:00

  • Biodiscovery from rare actinomycetes: an eco-taxonomical perspective.

    abstract::Microbial natural products, in particular, the ones produced by the members of the order Actinomycetales, will continue to represent an important route to the discovery of novel classes of bioactive compounds. As a result, the search for and discovery of lesser-known and/or novel actinomycetes is of significant intere...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-012-3898-2

    authors: Kurtböke DI

    更新日期:2012-03-01 00:00:00