Bacterial response to acetate challenge: a comparison of tolerance among species.

abstract：:Demand for novel antifungal drugs for medical and agricultural uses has been increasing because of the diversity of pathogenic fungi and the emergence of drug-resistant strains. Genomic resources for various living species, including pathogenic fungi, can be utilized to develop novel and effective antifungal compounds...

journal_title：Applied microbiology and biotechnology

authors： Marui J,Yoshimi A,Hagiwara D,Fujii-Watanabe Y,Oda K,Koike H,Tamano K,Ishii T,Sano M,Machida M,Abe K

更新日期：2010-08-01 00:00:00

abstract：:Uniform stable-isotope labeling of mammalian cells is achieved via a novel formulation of a serum-free cell culture medium that is based on stable-isotope-labeled autolysates and lipid extracts of various microbiological origin. Yeast autolysates allow complete replacement of individual amino acids and organic acids i...

journal_title：Applied microbiology and biotechnology

authors： Egorova-Zachernyuk TA,Bosman GJ,Degrip WJ

更新日期：2011-01-01 00:00:00

abstract：:A strong promoter increases transcription of the genes of interest and enhances the production of various valuable substances. For a hyperthermophilic archaeon Thermococcus onnurineus NA1, which can produce H2 from carbon monoxide oxidation, we searched for a novel endogenous strong promoter by transcriptome analysis ...

journal_title：Applied microbiology and biotechnology

authors： Lee SH,Kim MS,Jung HC,Lee J,Lee JH,Lee HS,Kang SG

更新日期：2015-05-01 00:00:00

abstract：:A recombinant oleate hydratase from Lysinibacillus fusiformis converted ricinoleic acid to a product, whose chemical structure was identified as the novel compound 10,12-dihydroxystearic acid by gas chromatograph/mass spectrometry, Fourier transform infrared, and nuclear magnetic resonance analysis. The reaction condi...

journal_title：Applied microbiology and biotechnology

authors： Seo MH,Kim KR,Oh DK

更新日期：2013-10-01 00:00:00

abstract：:The genus Aspergillus is widely used for the production of plant cell wall polysaccharide-degrading enzymes. The range of enzymes purified from these fungi covers nearly every function required for the complete degradation of cellulose, xyloglucan, xylan, galacto(gluco)mannan and pectin. This paper describes the Asper...

journal_title：Applied microbiology and biotechnology

authors： de Vries RP

更新日期：2003-03-01 00:00:00

abstract：:Extracellular manganese peroxidase (MnP) was purified from the compost extract of Agaricus bisporus using anion exchange chromatography, gel filtration and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Two forms (MnP1 and MnP2) were separated by isoelectric focusing and their isoelectric points...

journal_title：Applied microbiology and biotechnology

authors： Lankinen VP,Bonnen AM,Anton LH,Wood DA,Kalkkinen N,Hatakka A,Thurston CF

更新日期：2001-03-01 00:00:00

abstract：:Bacillus cereus is widely distributed food-borne pathogenic bacterium. Due to the harmness to human hearth and the generation of multidrug-resistant B. cereus, it is urgent to develop novel antimicrobial agents. Phage and phage endolysin were taken as novel antimicrobial substance for their specific lytic activity aga...

journal_title：Applied microbiology and biotechnology

authors： Peng Q,Yuan Y

更新日期：2018-09-01 00:00:00

abstract：:Paddy fields are a significant source of methane and contribute up to 20% of total methane emissions from wetland ecosystems. These inundated, anoxic soils featuring abundant nitrogen compounds and methane are an ideal niche for nitrate-dependent anaerobic methanotrophs. After 2 years of enrichment with a continuous s...

journal_title：Applied microbiology and biotechnology

authors： Vaksmaa A,Guerrero-Cruz S,van Alen TA,Cremers G,Ettwig KF,Lüke C,Jetten MSM

更新日期：2017-09-01 00:00:00

abstract：:Mitochondrial dysfunction in Saccharomyces cerevisiae was selected as a marker of ion penetration following carbon ion beam (CIB) irradiation. Respiration-deficient mutants were screened. Following confirmation of negligible spontaneous mutation, eight genetically stable S. cerevisiae respiration-deficient mutant stra...

journal_title：Applied microbiology and biotechnology

authors： Guo X,Zhang M,Gao Y,Cao G,Yang Y,Lu D,Li W

更新日期：2019-02-01 00:00:00

abstract：:Wood contains primary extractives, which are present in all woods, and secondary extractives, which are confined in certain wood species. Extractives in wood play a major role in wood-bonding processes, as they can contribute to or determine the bonding relevant properties of wood such as acidity and wettability. Ther...

journal_title：Applied microbiology and biotechnology

authors： Roffael E

更新日期：2016-02-01 00:00:00

abstract：:In an effort to produce ricinoleic acid (RA), an important natural raw material with great values as a petrochemical replacement, in Schizosaccharomyces pombe, we introduced Claviceps purpurea oleate Δ12-hydroxylase gene (CpFAH12) to S. pombe, putting it under the control of an inducible nmt1 promoter. However, RA was...

journal_title：Applied microbiology and biotechnology

authors： Yazawa H,Holic R,Kumagai H,Uemura H

更新日期：2013-09-01 00:00:00

abstract：:Fatty acid-binding protein 2 (Fabp2), which is involved in the transport of long-chain fatty acids, is widely studied in mammals. Nevertheless, the role of this protein in teleost fish is mostly unknown. Here, we produced a fabp2-/- zebrafish (KO) animal model. Compared with wild-type zebrafish (WT), KO had a markedly...

journal_title：Applied microbiology and biotechnology

authors： Zhao Y,Cao X,Fu L,Gao J

更新日期：2020-03-01 00:00:00

abstract：:Development of a butanologenic strain with high selectivity for butanol production is often proposed as a possible route for improving the economics of biobutanol production by solventogenic Clostridium species. The acetoacetate decarboxylase (aadc) gene encoding acetoacetate decarboxylase (AADC), which catalyzes the ...

journal_title：Applied microbiology and biotechnology

authors： Han B,Gopalan V,Ezeji TC

更新日期：2011-08-01 00:00:00

abstract：:Using genetic hybridization analysis, pulsed-field gel electrophoresis of chromosomal DNA and PCR/RFLP analysis of the MET2 gene, we reidentified 11 Champagne yeast strains. Two of them, SCPP and SC4, were found to belong to Saccharomyces bayanus var. uvarum and the remaining strains to S. cerevisiae. Strain ...

journal_title：Applied microbiology and biotechnology

authors： Naumov GI,Naumova ES,Aigle M,Masneuf I,Belarbi A

更新日期：2001-01-01 00:00:00

abstract：:The cDNA sequence of the gene for xylose isomerase from the rumen fungus Orpinomyces was elucidated by rapid amplification of cDNA ends. The 1,314-nucleotide gene was cloned and expressed constitutively in Saccharomyces cerevisiae. The deduced polypeptide sequence encoded a protein of 437 amino acids which showed the ...

journal_title：Applied microbiology and biotechnology

authors： Madhavan A,Tamalampudi S,Ushida K,Kanai D,Katahira S,Srivastava A,Fukuda H,Bisaria VS,Kondo A

更新日期：2009-04-01 00:00:00

abstract：:Cultures of Umbelopsis ramanniana (=Mucor ramannianus) were grown in fluid Sabouraud medium for 3 days, dosed with 0.23 mM benzo[f]quinoline, benzo[h]quinoline, or phenanthridine (benzo[c]quinoline), and incubated for another 18 days. Cultures were extracted and metabolites (66-75% of the UV absorbance) were separated...

journal_title：Applied microbiology and biotechnology

authors： Sutherland JB,Cross EL,Heinze TM,Freeman JP,Moody JD

更新日期：2005-05-01 00:00:00

abstract：:Lipase has been used widely in industry. In this study, we have constructed two recombinant Saccharomyces cerevisiae strains displaying two active lipases on the cell surface by cell surface engineering. The genes encoding Yarrowia lipolytica lipases Lip7 and Lip8 were fused with the gene encoding small binding subuni...

journal_title：Applied microbiology and biotechnology

authors： Liu WS,Pan XX,Jia B,Zhao HY,Xu L,Liu Y,Yan YJ

更新日期：2010-10-01 00:00:00

abstract：:Agriculture surplus were used as substrates to synthesize gamma-aminobutyric acid (GABA) by Lactobacillus plantarum DSM19463 for the manufacture of a functional beverage or as a novel application for dermatological purposes. Dilution of the grape must to 1 or 4% (w/v) of total carbohydrates favored higher cell yield a...

journal_title：Applied microbiology and biotechnology

authors： Di Cagno R,Mazzacane F,Rizzello CG,De Angelis M,Giuliani G,Meloni M,De Servi B,Gobbetti M

更新日期：2010-03-01 00:00:00

abstract：:Nanobiotechnology raises fascinating possibilities for new analytical assays in various fields such as bioelectronic assembly, biomechanics and sampling techniques, as well as in chips or micromachined devices. Recently, nanotechnology has greatly impacted biotechnological research with its potential applications in s...

journal_title：Applied microbiology and biotechnology

authors： Lee SJ,Lee SY

更新日期：2004-04-01 00:00:00

abstract：:The lysin gene (lysA2) of the Lactobacillus casei bacteriophage A2 was cloned and expressed in Escherichia coli. LysA2 is an endopeptidase that hydrolyzes the bond between the terminal D: -alanine of the peptidoglycan tetrapeptide and the aspartic acid residue that forms the bridge with the L: -lysine of a neighboring...

journal_title：Applied microbiology and biotechnology

authors： Ribelles P,Rodríguez I,Suárez JE

更新日期：2012-04-01 00:00:00

abstract：:Cytophaga hutchinsonii is a Gram-negative gliding bacterium, which can rapidly degrade crystalline cellulose via a novel strategy without any recognizable processive cellulases. Its mechanism of cellulose binding and degradation is still a mystery. In this study, the mutagenesis of C. hutchinsonii with the mariner-bas...

journal_title：Applied microbiology and biotechnology

authors： Ji X,Xu Y,Zhang C,Chen N,Lu X

更新日期：2012-10-01 00:00:00

abstract：:Cordyceps spp. is the herbal medication initially used in China and has been reported as the unique resource of cordycepin. Cordycepin exhibits many health benefits, including anti-photoaging and anti-pigmentation; therefore, it potentially is a bioactive ingredient of cosmetic products. In order to enrich cordycepin ...

journal_title：Applied microbiology and biotechnology

authors： Kunhorm P,Chaicharoenaudomrung N,Noisa P

更新日期：2019-02-01 00:00:00

abstract：:In this study, the lipoxygenase (ana-LOX) gene from Anabaena sp. PCC 7120 was successful expressed and secreted in Bacillus subtilis. Under the control of the P43 promoter, with a signal peptide from the B. subtilis 168 nprB gene, and facilitated by the molecular chaperone PrsA, the production of the recombinant ana-L...

journal_title：Applied microbiology and biotechnology

authors： Zhang C,Tao T,Ying Q,Zhang D,Lu F,Bie X,Lu Z

更新日期：2012-05-01 00:00:00

abstract：:The present study emphasised on the development of new mathematical models based on mass balance and stoichiometry of nitrogen removal in anammox hybrid reactor (AHR). The performance of AHR at varying hydraulic retention times (HRTs) and nitrogen loading rates (NLRs) revealed that nitrogen removal efficiency (NRE) in...

journal_title：Applied microbiology and biotechnology

authors： Tomar S,Gupta SK

更新日期：2015-11-01 00:00:00

abstract：:It was found that benzothiazole, 2-oxybenzothiazole and 2-benzothiazolesulphonate were degraded in activated sludge systems. 2-Mercaptobenzothiazole (MBT) was more resistant, although the first step in MBT degradation seemed to be transformation to the sulphonate form. At higher MBT concentrations, it was transformed ...

journal_title：Applied microbiology and biotechnology

authors： De Vos D,De Wever H,Verachtert H

更新日期：1993-07-01 00:00:00

abstract：:Mitigation of the methane (CH4) emission from ruminants is needed to decrease the environmental impact of ruminant animal production. Different plant materials and chemicals have been tested, but few are both effective and practical. Medicinal herbs contain biological compounds and antimicrobials that may be effective...

journal_title：Applied microbiology and biotechnology

authors： Wang J,Liu M,Wu Y,Wang L,Liu J,Jiang L,Yu Z

更新日期：2016-11-01 00:00:00

abstract：:An engineered yeast with emission of fluorescence from the cell surface was constructed. Cell surface engineering was applied to display a visible reporter molecule, green fluorescent protein (GFP). A glucose-inducible promoter GAPDH as a model promoter was selected to control the expression of the reporter gene in re...

journal_title：Applied microbiology and biotechnology

authors： Ye K,Shibasaki S,Ueda M,Murai T,Kamasawa N,Osumi M,Shimizu K,Tanaka A

更新日期：2000-07-01 00:00:00

abstract：:By using immobilized baker's yeast repeatedly in isooctane with occasional reactivation by cultivation, we succeeded in the preparation of an organic solvent-tolerant strain, named KK21, which could grow in the presence of isooctane. This is the first report on an organic solvent-tolerant strain from baker's yeast. St...

journal_title：Applied microbiology and biotechnology

authors： Kawamoto T,Kanda T,Tanaka A

更新日期：2001-05-01 00:00:00

abstract：:Escherichia coli K-12 was cultured under anaerobic conditions to form biofilm on carbon fiber electrodes in glucose-containing medium. The anodic current increased with the development of the biofilm and depended on the glucose concentration. Cyclic voltammetric results support the presence of a redox compound(s) excr...

journal_title：Applied microbiology and biotechnology

authors： Wang YF,Tsujimura S,Cheng SS,Kano K

更新日期：2007-10-01 00:00:00

abstract：:Phenylpropanoids as abundant, lignin-derived compounds represent sustainable feedstocks for biotechnological production processes. We found that the biotechnologically important soil bacterium Corynebacterium glutamicum is able to grow on phenylpropanoids such as p-coumaric acid, ferulic acid, caffeic acid, and 3-(4-h...

journal_title：Applied microbiology and biotechnology

authors： Kallscheuer N,Vogt M,Kappelmann J,Krumbach K,Noack S,Bott M,Marienhagen J

更新日期：2016-02-01 00:00:00