Regulation of Aspergillus genes encoding plant cell wall polysaccharide-degrading enzymes; relevance for industrial production.

abstract：:The activities of seven hydrolytic enzymes (L-alanine aminopeptidase, esterase, α-and β-glucosidase, phosphomonoesterase, phosphodiesterase, sulfatase) were monitored during 1 year in parallel and serial treatment units of the biological stage of a communal wastewater treatment plant. The spatial homogeneity of enzyme...

journal_title：Applied microbiology and biotechnology

authors： Kreutz JA,Böckenhüser I,Wacht M,Fischer K

更新日期：2016-08-01 00:00:00

abstract：:Porcine circovirus type 3 (PCV3) is an emerging swine pathogen associated with acute porcine dermatitis and nephropathy syndrome (PDNS)-like clinical signs, reproductive failure, and multisystemic inflammation. Current evidence shows that PCV3 is spread worldwide, and its high incidence may pose a threat to the global...

journal_title：Applied microbiology and biotechnology

authors： Jiang M,Guo J,Zhang G,Jin Q,Liu Y,Jia R,Wang A

更新日期：2020-07-01 00:00:00

abstract：:Protein translation, translocation, folding, processing, and secretion in eukaryotic cells are complex and not always straightforward processes, e.g., different routes of secretion and degradation exist. Formation of malfolded proteins in the endoplasmic reticulum (ER) can be one of the major bottlenecks for recombina...

journal_title：Applied microbiology and biotechnology

authors： Pfeffer M,Maurer M,Stadlmann J,Grass J,Delic M,Altmann F,Mattanovich D

更新日期：2012-03-01 00:00:00

abstract：:Chemical mutagenesis of the phototrophic green microalga Parietochloris incisa, producing high amounts of arachidonic acid (ARA), resulted in selection of a mutant deficient in ARA and rich in dihomo-γ-linolenic acid (DGLA) and thus ∆5 desaturase defective. The mutagenesis produced a nonsense mutation in the ∆5 desatu...

journal_title：Applied microbiology and biotechnology

authors： Iskandarov U,Khozin-Goldberg I,Cohen Z

更新日期：2011-04-01 00:00:00

abstract：:A Lactobacillus brevis strain with the ability to synthesize butanol from glucose was constructed by metabolic engineering. The genes crt, bcd, etfB, etfA, and hbd, composing the bcs-operon, and the thl gene encode the enzymes of the lower part of the clostridial butanol pathway (crotonase, butyryl-CoA-dehydrogenase, ...

journal_title：Applied microbiology and biotechnology

authors： Berezina OV,Zakharova NV,Brandt A,Yarotsky SV,Schwarz WH,Zverlov VV

更新日期：2010-06-01 00:00:00

abstract：:Two lab-scale plug flow activated sludge reactors were run in parallel for 4 months at 30 and 55 degrees C. Research focussed on: (1) COD (chemical oxygen demand) removal, (2) effluent turbidity at both temperatures, (3) the origin of effluent colloidal material and (4) the possible role of protozoa on turbidity level...

journal_title：Applied microbiology and biotechnology

authors： Vogelaar JC,van Lier JB,Klapwijk B,de Vries MC,Lettinga G

更新日期：2002-06-01 00:00:00

abstract：:An efficient fedbatch process for the production of Lactobacillus kefir DSM 20587 cells was developed. An improvement in space time yield of 270% (3.7 g(DCW) l(-1)-day(-1)) and in final enzyme activity of 440% (9.1 U/ml) was achieved on a 150 l scale by controlling the oxygen transfer rate to 7--9 mmol l(-1) h(-1). Th...

journal_title：Applied microbiology and biotechnology

authors： Pfruender H,Amidjojo M,Hang F,Weuster-Botz D

更新日期：2005-06-01 00:00:00

abstract：:The active efflux system contributing to the solvent tolerance of Pseudomonas putida S12 was characterized physiologically. The mutant P. putida JK1, which lacks the active efflux system, was compared with the wild-type organism. None of 20 known substrates of common multi-drug-resistant pumps had a stronger growth-in...

journal_title：Applied microbiology and biotechnology

authors： Isken S,De Bont JA

更新日期：2000-11-01 00:00:00

abstract：:Malaria is a parasitic, mosquito-borne, infectious disease that threatens nearly half of the global population. The last decade has seen a dramatic drop in the number of malaria-related deaths because of vector control methods and anti-malarial drugs. Unfortunately, this strategy is not sustainable because of the emer...

journal_title：Applied microbiology and biotechnology

authors： Gregory JA,Mayfield SP

更新日期：2014-03-01 00:00:00

abstract：:Psc-AFP, isolated from the seeds of Psoralea corylifolia L., is an antimicrobial protein with trypsin inhibitor activity. Its encoding gene was cloned by 3'- rapid amplification of cDNA ends (RACE) combined with Y-shaped adaptor-dependent extension (YADE) method. The gene Psc-AFP encodes a protein of 203 amino acids w...

journal_title：Applied microbiology and biotechnology

authors： Luo XM,Xie CJ,Wang,Wei YM,Cai J,Cheng SS,Yang X-,Sui A-

更新日期：2017-02-01 00:00:00

abstract：:A general procedure for the high yield immobilization of enzymes with the help of specific anti-enzyme antibodies is described. Polyclonal antibodies were raised against Aspergillus niger glucose oxidase and horseradish peroxidase in rabbits and the gamma globulin (IgG) fraction from the immune sera isolated by ammoni...

journal_title：Applied microbiology and biotechnology

authors： Farooqi M,Sosnitza P,Saleemuddin M,Ulber R,Scheper T

更新日期：1999-09-01 00:00:00

abstract：:A recent study of the UV-generated glucose-utilizing mutant Ralstonia eutropha G⁺1 comprising transcriptomic and proteomic analyses revealed clear evidence that glucose is transported by the N-acetylglucosamine-specific phosphotransferase system (PTS(Nag)), which is overexpressed in this mutant due to a derepression o...

journal_title：Applied microbiology and biotechnology

authors： Raberg M,Kaddor C,Kusian B,Stahlhut G,Budinova R,Kolev N,Bowien B,Steinbüchel A

更新日期：2012-08-01 00:00:00

abstract：:Traditional temperature-sensitive systems use either heat shock (40-42 °C) or cold shock (15-23 °C) to induce gene expression at temperatures that are not the optimal temperature for host cell growth (37 °C). This impacts the overall productivity and yield by disturbing cell growth and cellular metabolism. Here, we ha...

journal_title：Applied microbiology and biotechnology

authors： Jiang X,Zhang H,Yang J,Liu M,Feng H,Liu X,Cao Y,Feng D,Xian M

更新日期：2013-06-01 00:00:00

abstract：:Physico-chemical parameters, hydrological conditions, and microbial interactions can affect the growth and persistence of cyanobacteria, but the interacting effects among these bloom-forming factors are still poorly known. This hampers our capacity to predict the occurrence of cyanobacterial bloom accurately. Here, we...

journal_title：Applied microbiology and biotechnology

authors： Song H,Xu J,Lavoie M,Fan X,Liu G,Sun L,Fu Z,Qian H

更新日期：2017-02-01 00:00:00

abstract：:Possessing three circular chromosomes is a distinct genomic characteristic of Burkholderia cenocepacia AU 1054, a clinically important pathogen in cystic fibrosis. In this study, base composition, codon usage and functional role category were analyzed in the B. cenocepacia AU 1054 genome. Although no bias in the base ...

journal_title：Applied microbiology and biotechnology

authors： Yuan J,Yang M,Ren J,Fu B,Jiang F,Zhang X

更新日期：2014-01-01 00:00:00

abstract：:In an effort to produce ricinoleic acid (RA), an important natural raw material with great values as a petrochemical replacement, in Schizosaccharomyces pombe, we introduced Claviceps purpurea oleate Δ12-hydroxylase gene (CpFAH12) to S. pombe, putting it under the control of an inducible nmt1 promoter. However, RA was...

journal_title：Applied microbiology and biotechnology

authors： Yazawa H,Holic R,Kumagai H,Uemura H

更新日期：2013-09-01 00:00:00

abstract：:Biodiesel production by microalgae with photosynthetic CO2 biofixation is thought to be a feasible way in the field of bioenergy and carbon emission reduction. Knowledge of the carbon-concentrating mechanism plays an important role in improving microalgae carbon fixation efficiency. However, little information is avai...

journal_title：Applied microbiology and biotechnology

authors： Fan J,Xu H,Luo Y,Wan M,Huang J,Wang W,Li Y

更新日期：2015-03-01 00:00:00

abstract：:Guanosine 5'-triphosphate (GTP) is the key substrate for biosynthesis of guanosine 5'-diphosphate (GDP)-L-fucose. In this study, improvement of GDP-L-fucose production was attempted by manipulating the biosynthetic pathway for guanosine nucleotides in recombinant Escherichia coli-producing GDP-L-fucose. The effects of...

journal_title：Applied microbiology and biotechnology

authors： Lee WH,Shin SY,Kim MD,Han NS,Seo JH

更新日期：2012-03-01 00:00:00

abstract：:Monascus purpureus, a pigment-producing ascomycetous fungus, has been traditionally used for red rice preparation using solid-state fermentation. The objective of this study was to develop an improved pigment-producing strain of M. purpureus MTCC 1090 through genome shuffling followed by detailed analytical estimation...

journal_title：Applied microbiology and biotechnology

authors： Ghosh S,Dam B

更新日期：2020-12-01 00:00:00

abstract：:A cost-effective conversion of lignocellulosic biomass into bioethanol requires that the xylose released from the hemicellulose fraction (20-40% of biomass) can be fermented. Baker's yeast, Saccharomyces cerevisiae, efficiently ferments glucose but it lacks the ability to ferment xylose. Xylose-fermenting yeast such a...

journal_title：Applied microbiology and biotechnology

authors： Hou X

更新日期：2012-04-01 00:00:00

abstract：:Although the niche differentiation of anammox bacteria has been extensively observed in a lab-scale reactor, the inherent mechanism for this ecological phenomenon is still elusive. Here, we combined the long-term reactor operation, genome-centered metagenome, and metatranscriptome analyses to gain insight into the sub...

journal_title：Applied microbiology and biotechnology

authors： Zhao Y,Feng Y,Chen L,Niu Z,Liu S

更新日期：2019-10-01 00:00:00

abstract：:Clostridium acetobutylicum is an important industrial microorganism for n-butanol bioproduction, and its transcription factor AbrB0310 regulates various important cellular processes. However, the roles of two abrB homologues, abrB1941 and abrB3647, have not been determined because they appear inactive during transcrip...

journal_title：Applied microbiology and biotechnology

authors： Xue Q,Yang Y,Chen J,Chen L,Yang S,Jiang W,Gu Y

更新日期：2016-11-01 00:00:00

abstract：:D-Amino acids exist widely in microbes, plants, animals, and food and can be applied in pharmaceutical, food, and cosmetics. Because of their widespread applications in industry, D-amino acids have recently received more and more attention. Enzymes including D-hydantoinase, N-acyl-D-amino acid amidohydrolase, D-amino ...

journal_title：Applied microbiology and biotechnology

authors： Gao X,Ma Q,Zhu H

更新日期：2015-04-01 00:00:00

abstract：:A genetically engineered microorganism (GEM) was designed to exemplify bacterial strains used for the production of biological material in industry. The recombinant DNA was located on a safety plasmid (pUC19). Survival and persistence of the GEM and its recombinant DNA (rDNA) was determined in soil microcosms by using...

journal_title：Applied microbiology and biotechnology

authors： Henschke RB,Henschke EJ,Schmidt FR

更新日期：1991-05-01 00:00:00

abstract：:Nonadapted freshwater sediment slurries and sediment slurries adapted to dechlorinate 2,3-dichloropyridine (2,3-Cl2Pyd), 2,3-dichloroaniline (2,3-Cl2Anl), 2,3-dichlorophenol (2,3-Cl2PhOH), 3,5-dichloropyridine (3,5-Cl2Pyd), 3,5-dichloroaniline (3,5-Cl2Anl) and 3,5-dichlorophenol (3,5-Cl2PhOH) were studied to determine...

journal_title：Applied microbiology and biotechnology

authors： Liu SM,Jones WJ

更新日期：1995-08-01 00:00:00

abstract：:MoHrip2, identified from Magnaporthe oryzae as an elicitor, can activate plant defense responses either in the form of recombinant protein in vitro or ectopic expressed protein in rice. However, its intrinsic function in the infective interaction of M. oryzae-rice is largely unknown. Here, we found that mohrip2 expres...

journal_title：Applied microbiology and biotechnology

authors： Nie H,Zhang L,Zhuang H,Yang X,Qiu D,Zeng H

更新日期：2019-08-01 00:00:00

abstract：:A newly isolated strain of Cunninghamella echinulata grown on glucose produced significant quantities of biomass and cellular lipids in media with high C/N ratio. The oil yield from glucose consumed increased after nitrogen exhaustion in the growth medium, but gamma-linolenic acid (GLA) content in cellular oil systema...

journal_title：Applied microbiology and biotechnology

authors： Gema H,Kavadia A,Dimou D,Tsagou V,Komaitis M,Aggelis G

更新日期：2002-03-01 00:00:00

abstract：:The TonB system functions in iron transport and has been identified in certain Gram-negative bacteria. Recently, we reported three TonB systems in the Aeromonas hydrophila Chinese epidemic strain NJ-35, but the functions of these systems have not been thoroughly elucidated to date. In this study, we investigated the r...

journal_title：Applied microbiology and biotechnology

authors： Dong Y,Geng J,Liu J,Pang M,Awan F,Lu C,Liu Y

更新日期：2019-05-01 00:00:00

abstract：:Escherichia coli BL21, expressing a quintuple mutant of P450(BM-3), oxyfunctionalizes alpha-pinene in an NADPH-dependent reaction to alpha-pinene oxide, verbenol, and myrtenol. We optimized the whole-cell biocatalyst by integrating a recombinant intracellular NADPH regeneration system through co-expression of a glucos...

journal_title：Applied microbiology and biotechnology

authors： Schewe H,Kaup BA,Schrader J

更新日期：2008-02-01 00:00:00

abstract：:The desire to reduce dependence on the ever diminishing fossil fuel reserves coupled with the impetus towards green energy has seen increased research in biofuels as alternative sources of energy. Lignocellulose materials are one of the most promising feedstocks for advanced biofuels production. However, their utilisa...

journal_title：Applied microbiology and biotechnology

authors： Kudanga T,Le Roes-Hill M

更新日期：2014-08-01 00:00:00