Enrichment of cordycepin for cosmeceutical applications: culture systems and strategies.

abstract：:Drug resistance occurrence is a global healthcare concern responsible for the increased morbidity and mortality in hospitals, time of hospitalisation and huge financial loss. The failure of the most antibiotics to kill "superbugs" poses the urgent need to develop innovative strategies aimed at not only controlling bac...

journal_title：Applied microbiology and biotechnology

authors： Bassegoda A,Ivanova K,Ramon E,Tzanov T

更新日期：2018-03-01 00:00:00

abstract：:Orange peel waste (OPW), the primary byproduct of the juice extraction process, is annually generated in massive amounts (21 Mton), and its aqueous extraction in biorefining operations yields a liquid fraction, referred to as orange peel extract (OPE). Although OPE contains significant amounts of easily assimilable ca...

journal_title：Applied microbiology and biotechnology

authors： Carota E,Petruccioli M,D'Annibale A,Gallo AM,Crognale S

更新日期：2020-05-01 00:00:00

abstract：:This paper introduces a new type of system to simulate conditions in the large intestine. This system combines removal of metabolites and water with peristaltic mixing to obtain and handle physiological concentrations of microorganisms, dry matter and microbial metabolites. The system has been designed to be complemen...

journal_title：Applied microbiology and biotechnology

authors： Minekus M,Smeets-Peeters M,Bernalier A,Marol-Bonnin S,Havenaar R,Marteau P,Alric M,Fonty G,Huis in't Veld JH

更新日期：1999-12-01 00:00:00

abstract：:The adhesion properties of 14 Streptococcus salivarius strains to mucus (HT29-MTX) and non-mucus secreting (Caco-2/TC7) human intestinal epithelial cells were investigated. Ability to adhere to these two eukaryotic cell lines greatly differs between strains. The presence of mucus played a major factor in adhesion, lik...

journal_title：Applied microbiology and biotechnology

authors： Chaffanel F,Charron-Bourgoin F,Soligot C,Kebouchi M,Bertin S,Payot S,Le Roux Y,Leblond-Bourget N

更新日期：2018-03-01 00:00:00

abstract：:Streptomyces lividans IAF18, obtained by homologous cloning, is capable of over-producing XlnA. To investigate the possibility of the expression of foreign genes, various coding regions of the xylanase A gene (xlnA) were analysed. Expression/secretion vectors were constructed containing the regulatory elements of xlnA...

journal_title：Applied microbiology and biotechnology

authors： Paradis FW,Shareck F,Dupont C,Kluepfel D,Morosoli R

更新日期：1996-06-01 00:00:00

abstract：:Epilactose (4-O-β-D-galactopyranosyl-D-mannose), an epimer of lactose, is a rare disaccharide existing extremely small quantities in heat-treated milk, in which epilactose is produced by non-enzymatic catalysis from lactose. This disaccharide is a kind of non-digestible carbohydrate, has a good prebiotic effect, and p...

journal_title：Applied microbiology and biotechnology

authors： Mu W,Li Q,Fan C,Zhou C,Jiang B

更新日期：2013-03-01 00:00:00

abstract：:A halotolerant, alkaliphilic dissimilatory Fe(III)-reducing bacterium, strain SFB, was isolated from salt flat sediments collected from Soap Lake, WA. 16S ribosomal ribonucleic acid gene sequence analysis identified strain SFB as a novel Bacillus sp. most similar to Bacillus agaradhaerens (96.7% similarity). Strain SF...

journal_title：Applied microbiology and biotechnology

authors： Pollock J,Weber KA,Lack J,Achenbach LA,Mormile MR,Coates JD

更新日期：2007-12-01 00:00:00

abstract：:Alkalophilic Bacillus sp. KSM-K16 produced three alkaline proteases, as detected by polyacrylamide gel electrophoresis (PAGE). The major protease, designated M protease, was recently purified to homogeneity and its properties were characterized. In the present study, two minor proteases, designated H protease and N pr...

journal_title：Applied microbiology and biotechnology

authors： Kobayashi T,Hakamada Y,Hitomi J,Koike K,Ito S

更新日期：1996-03-01 00:00:00

abstract：:His-Asn-His (HNH) proteins are a very common family of small nucleic acid-binding proteins that are generally associated with endonuclease activity and are found in all kingdoms of life. Although HNH endonucleases from mesophiles have been widely investigated, the biochemical functions of HNH endonucleases from thermo...

journal_title：Applied microbiology and biotechnology

authors： Zhang L,Huang Y,Xu D,Yang L,Qian K,Chang G,Gong Y,Zhou X,Ma K

更新日期：2016-09-01 00:00:00

abstract：:Inhibitory compounds liberated from lignocellulose pretreatment are representative toxic chemicals that repress microbial growth and metabolism. A tolerant strain of the industrial yeast Saccharomyces cerevisiae is able to detoxify a major class of toxic compounds while producing ethanol. Knowledge on the yeast tolera...

journal_title：Applied microbiology and biotechnology

authors： Liu ZL,Huang X,Zhou Q,Xu J

更新日期：2019-07-01 00:00:00

abstract：:A simple and stable immobilization of a laccase from Pleurotus ostreatus was obtained through genetic fusion with a self-assembling and adhesive class I hydrophobin. The chimera protein was expressed in Pichia pastoris and secreted into the culture medium. The crude culture supernatant was directly used for coatings o...

journal_title：Applied microbiology and biotechnology

authors： Sorrentino I,Giardina P,Piscitelli A

更新日期：2019-04-01 00:00:00

abstract：:Aspergillus niger var. awamori was previously transformed with a vector designed to express a fused glucoamylase-prochymosin gene and bearing the Neurospora crassa pyr4 gene as a selectable marker. Mutant strains that overproduced the glucoamylase-prochymosin fusion protein were derived from one of the transformants. ...

journal_title：Applied microbiology and biotechnology

authors： Ward M,Wilson LJ,Kodama KH

更新日期：1993-08-01 00:00:00

abstract：:Some strains belonging to the genera Citrobacter and Enterobacter have been reported to produce chitin/chitosan-like bioflocculants (BFs) from acetate. In this study, to investigate the distribution of the BF-producing potential in the genus Citrobacter and to screen stably and highly BF-producing strains, we obtained...

journal_title：Applied microbiology and biotechnology

authors： Kimura K,Inoue T,Kato D,Negoro S,Ike M,Takeo M

更新日期：2013-11-01 00:00:00

abstract：:The basidiomycetous yeast genus Pseudozyma produce large amounts of mannosylerythritol lipids (MELs), which are biosurfactants. A few Pseudozyma strains produce mono-acylated MEL as a minor compound using excess glucose as the sole carbon source. Mono-acylated MEL shows higher hydrophilicity than di-acylated MEL and h...

journal_title：Applied microbiology and biotechnology

authors： Saika A,Utashima Y,Koike H,Yamamoto S,Kishimoto T,Fukuoka T,Morita T

更新日期：2018-02-01 00:00:00

abstract：:Discovery of keratin-degrading enzymes from fungi and bacteria has primarily focused on finding one protease with efficient keratinase activity. Recently, an investigation was conducted of all keratinases secreted from a fungus known to grow on keratinaceous materials, such as feather, horn, and hooves. The study demo...

journal_title：Applied microbiology and biotechnology

authors： Lange L,Huang Y,Busk PK

更新日期：2016-03-01 00:00:00

abstract：:Biosynthesis of Ds(+)-threo-isocitric acid from ethanol in the Yarrowia lipolytica batch and repeated-batch cultures was studied. Repeated-batch cultivation was found to provide for a good biosynthetic efficiency of the producer for as long as 748 h, probably due to maintenance of high activities of enzymes involved i...

journal_title：Applied microbiology and biotechnology

authors： Morgunov IG,Kamzolova SV,Karpukhina OV,Bokieva SB,Inozemtsev AN

更新日期：2019-04-01 00:00:00

abstract：:Non-model yeasts within basidiomycetes have considerable importance in agriculture, industry, and environment, but they are not as well studied as ascomycetous yeasts. Serving as a basic technique, nuclear DNA staining is widely used in physiology, ecology, cell biology, and genetics. However, it is unclear whether th...

journal_title：Applied microbiology and biotechnology

authors： Zhang N,Fan Y,Li C,Wang Q,Leksawasdi N,Li F,Wang S

更新日期：2018-05-01 00:00:00

abstract：:(S)-Hydroxynitrile lyase from Manihot esculenta (MeHNL) was shown for the first time to be able to catalyze the enantioselective transcyanation of acetyltrimethylsilane (ATMS) with acetone cyanohydrin to form (S)-2-trimethylsilyl-2-hydroxyl-propionitrile in an aqueous/organic biphasic system. To better understand the ...

journal_title：Applied microbiology and biotechnology

authors： Xu R,Zong MH,Liu YY,He J,Zhang YY,Lou WY

更新日期：2004-11-01 00:00:00

abstract：:Chitin was purified from Penaeus monodon and Crangon crangon shells using a two-stage fermentation process with anaerobic deproteination followed by decalcification through homofermentative lactic acid fermentation. Deproteinating enrichment cultures from sewage sludge and ground meat (GM) were used with a proteolytic...

journal_title：Applied microbiology and biotechnology

authors： Xu Y,Gallert C,Winter J

更新日期：2008-06-01 00:00:00

abstract：:L-Ribose is a rare and expensive sugar that can be used as a precursor for the production of L-nucleoside analogues, which are used as antiviral drugs. In this work, we describe a novel way of producing L-ribose from the readily available raw material L-arabinose. This was achieved by introducing L-ribose isomerase ac...

journal_title：Applied microbiology and biotechnology

authors： Helanto M,Kiviharju K,Granström T,Leisola M,Nyyssölä A

更新日期：2009-05-01 00:00:00

abstract：:Bacillus pumilus mutants were generated by targeted deletion of a set of genes eventually facilitating genetic handling and assuring biological containment. The well-defined and stable mutants do not form functional endospores due to the deletion of yqfD, an essential sporulation gene; they are affected in DNA repair,...

journal_title：Applied microbiology and biotechnology

authors： Wemhoff S,Meinhardt F

更新日期：2013-09-01 00:00:00

abstract：:Although the de novo biosynthetic mechanism of 3-hydroxypropionic acid (3-HP) in glycerol-fermenting microorganisms is still unclear, the propanediol utilization protein (PduP) of Lactobacillus species has been suggested to be a key enzyme in this regard. To verify this hypothesis, a pduP gene from Lactobacillus reute...

journal_title：Applied microbiology and biotechnology

authors： Luo LH,Seo JW,Baek JO,Oh BR,Heo SY,Hong WK,Kim DH,Kim CH

更新日期：2011-02-01 00:00:00

abstract：:The oxidizing activity of CYP109B1 from Bacillus subtilis was reconstituted in vitro with various artificial redox proteins including putidaredoxin reductase and putidaredoxin from Pseudomonas putida, truncated bovine adrenodoxin reductase and adrenodoxin, flavodoxin reductase and flavodoxin from Escherichia coli, and...

journal_title：Applied microbiology and biotechnology

authors： Girhard M,Klaus T,Khatri Y,Bernhardt R,Urlacher VB

更新日期：2010-06-01 00:00:00

abstract：:The alphaproteobacterial Roseobacter clade (Rhodobacterales) is one of the most important global players in carbon and sulfur cycles of marine ecosystems. The remarkable metabolic versatility of this bacterial lineage provides access to diverse habitats and correlates with a multitude of extrachromosomal elements. Fou...

journal_title：Applied microbiology and biotechnology

authors： Petersen J,Frank O,Göker M,Pradella S

更新日期：2013-04-01 00:00:00

abstract：:The production of biological indicators involving bacterial sporulation and multi-step downstream processes has been described. The goal of the present work was to use fermented material as the final product in a biological indicator, thereby reducing processing steps and costs. The performance of three different inex...

journal_title：Applied microbiology and biotechnology

authors： Sella SR,Guizelini BP,Zanello PH,Vandenberghe LP,Ribeiro CA,Minozzo JC,Soccol CR

更新日期：2012-01-01 00:00:00

abstract：:Microbial cell factories offer an economic approach for synthesizing "natural'" aromatic flavor compounds. During their fermentation process, the inefficient synthesis pathway and product cytotoxicity are the major barriers to the high-level production. This study combined metabolic engineering and tolerance engineeri...

journal_title：Applied microbiology and biotechnology

authors： Wang Y,Zhang Z,Lu X,Zong H,Zhuge B

更新日期：2020-12-01 00:00:00

abstract：:A genetically engineered microorganism (GEM) was designed to exemplify bacterial strains used for the production of biological material in industry. The recombinant DNA was located on a safety plasmid (pUC19). Survival and persistence of the GEM and its recombinant DNA (rDNA) was determined in soil microcosms by using...

journal_title：Applied microbiology and biotechnology

authors： Henschke RB,Henschke EJ,Schmidt FR

更新日期：1991-05-01 00:00:00

abstract：:It was found that benzothiazole, 2-oxybenzothiazole and 2-benzothiazolesulphonate were degraded in activated sludge systems. 2-Mercaptobenzothiazole (MBT) was more resistant, although the first step in MBT degradation seemed to be transformation to the sulphonate form. At higher MBT concentrations, it was transformed ...

journal_title：Applied microbiology and biotechnology

authors： De Vos D,De Wever H,Verachtert H

更新日期：1993-07-01 00:00:00

abstract：:Wastewater produced by the debittering process of green olives (GOW) is rich in polyphenolics and presents high chemical oxygen demand and alkalinity values. Eight white-rot fungi ( Abortiporus biennis, Dichomitus squalens, Inonotus hispidus, Irpex lacteus, Lentinus tigrinus, Panellus stipticus, Pleurotus ostreatus an...

journal_title：Applied microbiology and biotechnology

authors： Aggelis G,Ehaliotis C,Nerud F,Stoychev I,Lyberatos G,Zervakis GI

更新日期：2002-07-01 00:00:00

abstract：:Low-energy ions exist widely in the natural world. People had neglected the interaction between low-energy ions and material; it was even more out of the question to study the relation of low-energy ions and the complicated organism until the biological effects of low-energy ion implantation were discovered in 1989. N...

journal_title：Applied microbiology and biotechnology

authors： Gu SB,Li SC,Feng HY,Wu Y,Yu ZL

更新日期：2008-02-01 00:00:00