Abstract：
:A genetically engineered microorganism (GEM) was designed to exemplify bacterial strains used for the production of biological material in industry. The recombinant DNA was located on a safety plasmid (pUC19). Survival and persistence of the GEM and its recombinant DNA (rDNA) was determined in soil microcosms by using different monitoring methods, including the polymerase-chain reaction, to amplify and detect the specific rDNA. Depending on nutritional status, both the GEM and its rDNA had disappeared within 16 (amended soil) or 28 days (non-amended soil) with a limit of detection of 5 cells/g soil and 20 fg DNA/g soil.
journal_name
Appl Microbiol Biotechnoljournal_title
Applied microbiology and biotechnologyauthors
Henschke RB,Henschke EJ,Schmidt FRdoi
10.1007/BF00184696subject
Has Abstractpub_date
1991-05-01 00:00:00pages
247-52issue
2eissn
0175-7598issn
1432-0614journal_volume
35pub_type
杂志文章abstract：:In this work, the production of 1,3-propanediol from glucose and molasses was studied in a two-step process using two recombinant microorganisms. The first step of the process is the conversion of glucose or other sugar into glycerol by the metabolic engineered Saccharomyces cerevisiae strain HC42 adapted to high (>20...
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