Abstract：
:Fermentation-derived products are in greater demand to meet the increasing global market as well as to overcome environmental problems. In this work, Escherichia coli has been metabolically engineered with acrylate pathway genes from Clostridium propionicum for the conversion of D-lactic acid to propionic acid. The introduced synthetic pathway consisted of seven genes encoding the enzymes propionate CoA-transferase (Pct), lactoyl-CoA dehydratase (Lcd) and acryloyl-CoA reductase (Acr). The engineered strain synthesised propionic acid at a concentration of 3.7 ± 0.2 mM upon fermentation on glucose. This low production level could be attributed to the low activity of the recombinant enzymes in particular the rate-limiting enzyme, Acr. Interestingly, the recombinant pathway caused an increased lactate production in E. coli with a yield of 1.9 mol/mol of glucose consumed along with a decrease in other by-products. Down-regulation of the pfl (pyruvate formate lyase) genes and a possible inhibition of Pfl activity by the acrylate pathway intermediate, acryloyl-CoA, could have reduced carbon flow to the Pfl pathway with a concomitant increase in lactate production. This study reports a novel way of synthesising propionic acid by employing a non-native, user-friendly organism through metabolic engineering.
journal_name
Appl Microbiol Biotechnoljournal_title
Applied microbiology and biotechnologyauthors
Kandasamy V,Vaidyanathan H,Djurdjevic I,Jayamani E,Ramachandran KB,Buckel W,Jayaraman G,Ramalingam Sdoi
10.1007/s00253-012-4274-ysubject
Has Abstractpub_date
2013-02-01 00:00:00pages
1191-200issue
3eissn
0175-7598issn
1432-0614journal_volume
97pub_type
杂志文章abstract：:Biodegradation can achieve complete and cost-effective elimination of aromatic pollutants through harnessing diverse microbial metabolic processes. Aromatics biodegradation plays an important role in environmental cleanup and has been extensively studied since the inception of biodegradation. These studies, however, a...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章,评审
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abstract：:Mannitol is a natural low-calorie sugar alcohol produced by certain (micro)organisms applicable in foods for diabetics due to its zero glycemic index. In this work, we evaluated mannitol production and yield by the fruit origin strain Fructobacillus tropaeoli CRL 2034 using response surface methodology with central co...
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pub_type： 杂志文章,收录出版,评审
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更新日期：2002-10-01 00:00:00
abstract：:Microbial transglutaminase (MTG) from Streptomyces mobaraensis has been widely used for crosslinking proteins in order to acquire products with improved properties. To improve the yield and enable a facile and efficient purification process, recombinant vectors, harboring various heterologous signal peptide-encoding f...
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pub_type： 杂志文章,评审
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pub_type： 杂志文章
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更新日期：2015-05-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/BF00903777
更新日期：1990-01-01 00:00:00
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journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-010-2887-6
更新日期：2011-02-01 00:00:00
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journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-014-5535-8
更新日期：2014-05-01 00:00:00
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journal_title：Applied microbiology and biotechnology
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doi：10.1007/s00253-008-1425-2
更新日期：2008-05-01 00:00:00
abstract：:Since 2015, outbreaks of hepatitis-hydropericardium syndrome (HPS) caused by a novel genotype of fowl adenovirus 4 (FAdV-4) infection have created serious economic losses in China. Given that other serotypes of hypervirulent FAdVs have also been reported in poultry around the world, a common ELISA for all serotypes wi...
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更新日期：2020-01-01 00:00:00
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journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-015-6855-z
更新日期：2015-12-01 00:00:00
abstract：:Nervonic acid (NA) is a major very long-chain monounsaturated fatty acid found in the white matter of mammalian brains, which plays a critical role in the treatment of psychotic disorders and neurological development. In the nature, NA has been synthesized by a handful plants, fungi, and microalgae. Although the metab...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s00253-018-8859-y
更新日期：2018-04-01 00:00:00
abstract：:Streptomyces viridochromogenes Tü494 produces the antibiotic phosphinothricyl-alanyl-alanine (Ptt). Ptt-non-producing mutants were isolated following N-methyl-N'-nitro-N-nitrosoguanidine (NTG) or UV light treatment of spore suspensions. In co-synthesis and bioconversion experiments the mutational blocks in the biosynt...
journal_title：Applied microbiology and biotechnology
pub_type： 临床试验,杂志文章,多中心研究
doi：10.1007/BF00169345
更新日期：1991-03-01 00:00:00
abstract：:Alcaligenes sp. A 7-2 immobilized on granular clay has been applied in a percolator to degrade 4-chlorophenol in sandy soil. Good adsorption rates on granular clay were achieved using cell suspensions with high titres and media at pH 8.0. The influence of various parameters such as aeration rate, pH, temperature, conc...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/BF00169634
更新日期：1991-08-01 00:00:00
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journal_title：Applied microbiology and biotechnology
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journal_title：Applied microbiology and biotechnology
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更新日期：2015-11-01 00:00:00
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更新日期：1998-02-01 00:00:00
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journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
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更新日期：2016-08-01 00:00:00
abstract：:Nine anaerobic promoters were cloned and constructed upstream of PHB synthesis genes phbCAB from Ralstonia eutropha for the micro- or anaerobic PHB production in recombinant Escherichia coli. Among the promoters, the one for alcohol dehydrogenase (PadhE) was found most effective. Recombinant E. coli JM 109 (pWCY09) ha...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
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更新日期：2009-03-01 00:00:00
abstract：:Using organically synthesized hydroxyalkanoate coenzyme A thioesters, the activities of two short-chain polyhydroxalkanoate (PHA) synthases were investigated--Ralstonia eutropha PHA synthase (a type I PHA synthase) and Ectothiorhodospira shaposhnikovii PHA synthase (a type III synthase). The results indicate that the ...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
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更新日期：2001-07-01 00:00:00
