Enhanced D-lactic acid production from renewable resources using engineered Lactobacillus plantarum.

abstract：:In the Candida antarctica lipase B-catalyzed hydrolysis of (R,S)-azolides derived from (R,S)-N-protected proline in water-saturated methyl tert-butyl ether (MTBE), high enzyme activity with excellent enantioselectivity (V (S) V (R) ...

journal_title：Applied microbiology and biotechnology

authors： Wu CH,Pen CW,Wang PY,Tsai SW

更新日期：2013-02-01 00:00:00

abstract：:Aminotransferases (ATs) are important biocatalysts for the synthesis of chiral amines because of their capability of introducing amino group into ketones or keto acids as well as their high enantioselectivity, high regioselectivity. Among all ATs, branched-chain amino acid aminotransferase (BCAT) can use branched-chai...

journal_title：Applied microbiology and biotechnology

authors： Zheng X,Cui Y,Li T,Li R,Guo L,Li D,Wu B

更新日期：2019-10-01 00:00:00

abstract：:Fermentative H(2) production microbial structure in an agitated granular sludge bed bioreactor was analyzed using fluorescence in situ hybridization (FISH) and polymerase chain reaction-denatured gradient gel electrophoresis (PCR-DGGE). This hydrogen-producing system was operated at four different hydraulic retention ...

journal_title：Applied microbiology and biotechnology

authors： Hung CH,Lee KS,Cheng LH,Huang YH,Lin PJ,Chang JS

更新日期：2007-06-01 00:00:00

abstract：:In an effort to produce ricinoleic acid (RA), an important natural raw material with great values as a petrochemical replacement, in Schizosaccharomyces pombe, we introduced Claviceps purpurea oleate Δ12-hydroxylase gene (CpFAH12) to S. pombe, putting it under the control of an inducible nmt1 promoter. However, RA was...

journal_title：Applied microbiology and biotechnology

authors： Yazawa H,Holic R,Kumagai H,Uemura H

更新日期：2013-09-01 00:00:00

abstract：:Protein turnover through de novo synthesis is critical for sustainable cellular functions. We previously found that glucose consumption rate in Corynebacterium glutamicum under anaerobic conditions increased at temperature higher than the upper limit of growth temperature. Here, we showed that production of lactic and...

journal_title：Applied microbiology and biotechnology

authors： Uchikura H,Ninomiya K,Takahashi K,Tsuge Y

更新日期：2020-05-01 00:00:00

abstract：:A cDNA fragment encoding the A catalytic domain of the Neocallimastix frontalis endoxylanase XYN3 was amplified and cloned by the polymerase chain reaction technique. The xyn3A DNA fragment was inserted between the Saccharomyces cerevisiae phosphoglycerate kinase gene promoter and terminator sequences on a multicopy e...

journal_title：Applied microbiology and biotechnology

authors： Durand R,Rascle C,Fèvre M

更新日期：1999-08-01 00:00:00

abstract：:Cyanophycin is non-ribosomally synthesized protein-like copolymer. Synthesis of cyanophycin is catalyzed by cyanophycin synthetase (CphA). In this study, a novel cyanophycin synthetase CphA49 belonging to NOR5 clade of Gammaproteobacteria was identified with primer-based screening from a deep-sea sediment metagenomic ...

journal_title：Applied microbiology and biotechnology

authors： Du J,Li L,Ding X,Hu H,Lu Y,Zhou S

更新日期：2013-10-01 00:00:00

abstract：:Chiral alcohols are valuable as diverse chemicals and synthetic intermediate materials. Phenylacetaldehyde reductase (PAR) is an enzyme that converts a wide variety of ketones into chiral alcohols with high optical purity. When an alcohol such as 2-propanol is used as a hydrogen donor, PAR itself will also mediate the...

journal_title：Applied microbiology and biotechnology

authors： Makino Y,Itoh N

更新日期：2014-05-01 00:00:00

abstract：:The anaerobic degradation of phenol was studied in a fed-batch culture. Nitrate was added as electron acceptor and phenol was provided three times, to a final concentration of 200 mg/l. Randomly amplified polymorphic DNA (RAPD) and terminal fraction fragment length polymorphism (T-RFLP) were used and compared in order...

journal_title：Applied microbiology and biotechnology

authors： Guieysse B,Wickström P,Forsman M,Mattiasson B

更新日期：2001-09-01 00:00:00

abstract：:The 3D structures of Fim H and PapG proteins complexed with the host carbohydrate receptor demonstrate that both utilize binding-pocket asparagines for contact or stabilization with the carbohydrate. Pretreatment of whole bacteria with asparaginase resulted in decreased fimbriae-mediated attachment to urinary epitheli...

journal_title：Applied microbiology and biotechnology

authors： Wehner MR,Koch SR,Kindinger L,Nelson N,Cowan MM

更新日期：2004-11-01 00:00:00

abstract：:Glycerol formation is vital for reoxidation of nicotinamide adenine dinucleotide (reduced form; NADH) under anaerobic conditions and for the hyperosmotic stress response in the yeast Saccharomyces cerevisiae. However, relatively few studies have been made on hyperosmotic stress under anaerobic conditions. To study the...

journal_title：Applied microbiology and biotechnology

authors： Modig T,Granath K,Adler L,Lidén G

更新日期：2007-05-01 00:00:00

abstract：:Rhodococcus erythropolis cells contain a large set of enzymes that allow them to carry out an enormous number of bioconversions and degradations. Oxidations, dehydrogenations, epoxidations, hydrolysis, hydroxylations, dehalogenations and desulfurisations have been reported to be performed by R. erythropolis cells or e...

journal_title：Applied microbiology and biotechnology

authors： de Carvalho CC,da Fonseca MM

更新日期：2005-06-01 00:00:00

abstract：:Gene amplification using dihydrofolate reductase gene (dhfr) and methotrexate (MTX) is widely used for recombinant protein production in mammalian cells and is typically conducted in DHFR-deficient Chinese hamster ovary (CHO) cell lines. Generation of DHFR-deficient cells can be achieved by an expression vector incorp...

journal_title：Applied microbiology and biotechnology

authors： Kang SY,Kim YG,Lee HW,Lee EG

更新日期：2015-12-01 00:00:00

abstract：:Endophytic actinobacteria, which exist in the inner tissues of living plants, have attracted increasing attention among taxonomists, ecologists, agronomists, chemists and evolutionary biologists. Numerous studies have indicated that these prolific actinobacteria appear to have a capacity to produce an impressive array...

journal_title：Applied microbiology and biotechnology

authors： Qin S,Xing K,Jiang JH,Xu LH,Li WJ

更新日期：2011-02-01 00:00:00

abstract：:Production of gluconic acid by cells of Aspergillus niger immobilized on polyurethane foam was studied in repeated-batch shake-flask and bubble-column fermentations. For passive immobilization, various amounts of polyurethane foam and spore suspension were tested in order to obtain a suitable combination for optimal c...

journal_title：Applied microbiology and biotechnology

authors： Vassilev NB,Vassileva MC,Spassova DI

更新日期：1993-06-01 00:00:00

abstract：:The production of botulinum neurotoxin A (BoNT/A) for therapeutic and cosmetic applications requires precise determination of batch potency, and the enzymatic activity of BoNT/A light chain is a crucial index that can be measured in vitro. We previously established a SNAP-25 chip-based assay using surface plasmon reso...

journal_title：Applied microbiology and biotechnology

authors： Lévêque C,Ferracci G,Maulet Y,Grand-Masson C,Seagar M,El Far O

更新日期：2015-05-01 00:00:00

abstract：:Microbial transglutaminase (MTG) from Streptomyces mobaraensis has been widely used for crosslinking proteins in order to acquire products with improved properties. To improve the yield and enable a facile and efficient purification process, recombinant vectors, harboring various heterologous signal peptide-encoding f...

journal_title：Applied microbiology and biotechnology

authors： Mu D,Lu J,Qiao M,Kuipers OP,Zhu J,Li X,Yang P,Zhao Y,Luo S,Wu X,Jiang S,Zheng Z

更新日期：2018-07-01 00:00:00

abstract：:It is well established that the choice of yeast used to perform wine fermentation significantly influences the sensory attributes of wines; different yeast species and strains impart different profiles of esters, volatile fatty acids, higher alcohols, and volatile sulphur compounds. Indeed, choice of yeast remains one...

journal_title：Applied microbiology and biotechnology

authors： Cordente AG,Solomon M,Schulkin A,Leigh Francis I,Barker A,Borneman AR,Curtin CD

更新日期：2018-07-01 00:00:00

abstract：:Thermochemical processing of biomass by fast pyrolysis provides a nonenzymatic route for depolymerization of biomass into sugars that can be used for the biological production of fuels and chemicals. Fermentative utilization of this bio-oil faces two formidable challenges. First is the fact that most bio-oil-associate...

journal_title：Applied microbiology and biotechnology

authors： Jarboe LR,Wen Z,Choi D,Brown RC

更新日期：2011-09-01 00:00:00

abstract：:A Gram-positive, red-pigment-producing bacterial strain, designated JS520 was isolated from the pristine sediment from the cave on mountain Miroc in Serbia. Strain was confirmed to belong to Streptomyces genus based on phenotypic and genetic analysis. Streptomyces sp. JS520 has the ability to produce exceptionally hig...

journal_title：Applied microbiology and biotechnology

authors： Stankovic N,Radulovic V,Petkovic M,Vuckovic I,Jadranin M,Vasiljevic B,Nikodinovic-Runic J

更新日期：2012-12-01 00:00:00

abstract：:Problems with deflocculation and solids separation in biological wastewater treatment systems are linked to fluctuations in physicochemical conditions. This study examined the composition of activated sludge bacterial communities in lab-scale sequencing batch reactors treating bleached kraft mill effluent, under trans...

journal_title：Applied microbiology and biotechnology

authors： Nadarajah N,Allen DG,Fulthorpe RR

更新日期：2010-11-01 00:00:00

abstract：:With advances in the development of DNA vaccines and gene therapy, there is a growing need for plasmid DNA with high quality for fundamental research and clinical trials. In this report, a scalable automated process for large-scale preparation of plasmid is described. This process is based on alkaline lysis and can be...

journal_title：Applied microbiology and biotechnology

authors： Li X,Jin H,Wu Z,Rayner S,Yin J,Yu Y,Zhang W,He Z,Wang C,Wang B

更新日期：2007-07-01 00:00:00

abstract：:Plant health is determined by the comprehensive effect of soil physicochemical and biological properties. In this study, we compared the chemical properties and microbiomes of the rhizosphere soils of healthy, Fusarium oxysporum-infected, and dead watermelon plants and attempted to assess their potential roles in plan...

journal_title：Applied microbiology and biotechnology

authors： Meng T,Wang Q,Abbasi P,Ma Y

更新日期：2019-02-01 00:00:00

abstract：:Phosphate (P)-solubilizing microorganisms as a group form an important part of the microorganisms, which benefit plant growth and development. Growth promotion and increased uptake of phosphate are not the only mechanisms by which these microorganisms exert a positive effect on plants. Microbially mediated solubilizat...

journal_title：Applied microbiology and biotechnology

authors： Vassilev N,Vassileva M,Nikolaeva I

更新日期：2006-06-01 00:00:00

abstract：:Microbially induced CaCO3 precipitation (MICP) can give concrete self-healing properties. MICP agents are typically bacterial endospores which are coated into shelled granules, infused into expanded clay, or embedded into superabsorbent polymer (SAP). When small cracks appear in the cured concrete, the encapsulation i...

journal_title：Applied microbiology and biotechnology

authors： Nielsen SD,Koren K,Löbmann K,Hinge M,Scoma A,Kjeldsen KU,Røy H

更新日期：2020-01-01 00:00:00

abstract：:Toluene dioxygenase (TDO) catalyzes asymmetric cis-dihydroxylation of aromatic compounds. To achieve high efficient biotransformation of benzene to benzene cis-diols, Pseudomonas putida KT2442, Pseudomonas stutzeri 1317, and Aeromonas hydrophila 4AK4 were used as hosts to express TDO gene tod. Plasmid pSPM01, a deriva...

journal_title：Applied microbiology and biotechnology

authors： Ouyang SP,Sun SY,Liu Q,Chen J,Chen GQ

更新日期：2007-02-01 00:00:00

abstract：:Various single-cell isolation techniques, including dilution, micromanipulation, flow cytometry, microfluidics, and compartmentalization, have been developed. These techniques can be used to cultivate previously uncultured microbes, to assess and monitor cell physiology and function, and to screen for novel microbiolo...

journal_title：Applied microbiology and biotechnology

authors： Ishii S,Tago K,Senoo K

更新日期：2010-05-01 00:00:00

abstract：:Dramatic increase in bacterial resistance towards conventional antibiotics emphasises the importance to identify novel, more potent antimicrobial therapies. Antimicrobial peptides (AMPs) have emerged as a promising new group to be evaluated in therapeutic intervention of infectious diseases. Here we describe a novel A...

journal_title：Applied microbiology and biotechnology

authors： Myhrman E,Håkansson J,Lindgren K,Björn C,Sjöstrand V,Mahlapuu M

更新日期：2013-04-01 00:00:00

abstract：:Pseudomonas strain CT14 was isolated from activated sludge. Strain CT14 contained a 55, 216 bp plasmid that was characterized by sequence analysis. The plasmid had a modular structure with 51 open reading frames (ORFs) that were distributed between two clearly demarcated domains. Domain I primarily contained genes for...

journal_title：Applied microbiology and biotechnology

authors： Bramucci M,Chen M,Nagarajan V

更新日期：2006-06-01 00:00:00

abstract：:A system for the controlled expression of a foreign gene in cultured tobacco cells (Nicotiana tabacum, BY2) by temperature shift was constructed. A 925-base-pair (bp) DNA fragment containing the 5'-flanking region of a low-molecular-mass heat-shock protein gene (HSP18.2) of Arabidopsis thaliana was inserted upstream o...

journal_title：Applied microbiology and biotechnology

authors： Yoshida K,Kasai T,Garcia MR,Sawada S,Shoji T,Shimizu S,Yamazaki K,Komeda Y,Shinmyo A

更新日期：1995-12-01 00:00:00