Development of an improved phenylacetaldehyde reductase mutant by an efficient selection procedure.

abstract：:The paper describes the preparation of new probiotic formulations based on chitosan-coated alginate microcapsules containing three different probiotic strains, Lactobacillus plantarum PBS067, Lactobacillus rhamnosus PBS070, and Bifidobacterium animalis subsp. lactis PBS075 taken individually and as a mixture of them. ...

journal_title：Applied microbiology and biotechnology

authors： D'Orazio G,Di Gennaro P,Boccarusso M,Presti I,Bizzaro G,Giardina S,Michelotti A,Labra M,La Ferla B

更新日期：2015-11-01 00:00:00

abstract：:Corynebacterium glutamicum CCTCC M201005 produces a novel polygalacturonic acid bioflocculant, REA-11, consisting of galacturonic acid as the main structural unit. A biosynthetic pathway of REA-11 in C. glutamicum CCTCC M201005 was proposed. Evidence for the biosynthetic pathway was provided by: (1) analyzing the resp...

journal_title：Applied microbiology and biotechnology

authors： Li Y,He N,Guan H,Du G,Chen J

更新日期：2003-12-01 00:00:00

abstract：:Wetlands contribute to 30% of global methane emissions due to an imbalance between microbial methane production and consumption. Methanogenesis and methanotrophy have mainly been studied separately, and little is known about their potential interactions in aquatic environments. To mimic the interaction between methane...

journal_title：Applied microbiology and biotechnology

authors： In 't Zandt MH,van den Bosch TJM,Rijkers R,van Kessel MAHJ,Jetten MSM,Welte CU

更新日期：2018-07-01 00:00:00

abstract：:Many hydrocarbon-contaminated environments are characterized by low or elevated temperatures, acidic or alkaline pH, high salt concentrations, or high pressure, Hydrocarbon-degrading microorganisms, adapted to grow and thrive in these environments, play an important role in the biological treatment of polluted extreme...

journal_title：Applied microbiology and biotechnology

authors： Margesin R,Schinner F

更新日期：2001-09-01 00:00:00

abstract：:Two sequencing batch reactors were synchronously operated to investigate the effect of manganese (II) (Mn(2+)) augmentation on aerobic granulation. Reactor 1 (R1) was added with 10 mg/L Mn(2+), while there was no Mn(2+) augmentation in reactor 2 (R2). Results showed that R1 had a faster granulation process than R2 and...

journal_title：Applied microbiology and biotechnology

authors： Huang L,Yang T,Wang W,Zhang B,Sun Y

更新日期：2012-03-01 00:00:00

abstract：:Dramatic increase in bacterial resistance towards conventional antibiotics emphasises the importance to identify novel, more potent antimicrobial therapies. Antimicrobial peptides (AMPs) have emerged as a promising new group to be evaluated in therapeutic intervention of infectious diseases. Here we describe a novel A...

journal_title：Applied microbiology and biotechnology

authors： Myhrman E,Håkansson J,Lindgren K,Björn C,Sjöstrand V,Mahlapuu M

更新日期：2013-04-01 00:00:00

abstract：:Phenotypic plasticity is the capacity to change the phenotype in response to different environments without alteration of the genotype. Despite sufficient evidence that microorganisms have a major role in the fitness and sickness of eukaryotes, there has been little research regarding microbial phenotypic plasticity. ...

journal_title：Applied microbiology and biotechnology

authors： Zheng X,Bai J,Ye M,Liu Y,Jin Y,He X

更新日期：2020-06-01 00:00:00

abstract：:Biosynthesis has emerged as a frontier technology for fabrication of functionally diverse nanoparticles that possess tremendous therapeutic implications. Various biological resources have already demonstrated their potential to produce nanoparticles with interesting features. Endophytic microbes live in a symbiotic re...

journal_title：Applied microbiology and biotechnology

authors： Rahman S,Rahman L,Khalil AT,Ali N,Zia D,Ali M,Shinwari ZK

更新日期：2019-03-01 00:00:00

abstract：:Site-saturation mutagenesis (SSM) has been used in directed evolution of proteins for a long time. As a special form of saturation mutagenesis, it involves individual randomization at a given residue with formation of all 19 amino acids. To date, the most efficient embodiment of SSM is a one-step PCR-based approach us...

journal_title：Applied microbiology and biotechnology

authors： Li A,Acevedo-Rocha CG,Reetz MT

更新日期：2018-07-01 00:00:00

abstract：:Biocides play an important role in the preservation of white mineral dispersions (WMD). Due to the occurrence of biocide-resistant bacteria and technical limitations in the use of biocides, new preservation strategies are required-like the enhancement of biocides by non-biocidal compounds. The aim of this study was to...

journal_title：Applied microbiology and biotechnology

authors： Di Maiuta N,Schwarzentruber P,Dow CS

更新日期：2011-01-01 00:00:00

abstract：:The influence of different amino acid substitutions in the nitrilase from Pseudomonas fluorescens EBC191 (NitA) on the catalytical activity and the ability to form amides was investigated. The enzyme variant Glu137Ala was constructed because glutamate residues homologous to Glu137 are highly conserved among different ...

journal_title：Applied microbiology and biotechnology

authors： Sosedov O,Stolz A

更新日期：2015-03-01 00:00:00

abstract：:Previous investigations have reported that bacterial suspension cultures grow to higher stationary concentrations in space flight than on Earth; however, none of these investigations included extensive ground controls under varied inertial conditions. This study includes extensive controls and cell-growth data taken a...

journal_title：Applied microbiology and biotechnology

authors： Kacena MA,Merrell GA,Manfredi B,Smith EE,Klaus DM,Todd P

更新日期：1999-02-01 00:00:00

abstract：:Production of (R)-3-hydroxybutyric acid [(R)-3-HB] by strain Halomonas sp. KM-1 under successive aeration conditions was investigated. The first aerobic condition allowed both cell growth and intracellular storage of poly-(R)-3-hydroxybutyric acid (PHB). The second microaerobic condition, achieved by reducing the cult...

journal_title：Applied microbiology and biotechnology

authors： Kawata Y,Kawasaki K,Shigeri Y

更新日期：2012-11-01 00:00:00

abstract：:The ω-3 fatty acid desaturase (ω3Des) is a key enzyme in the biosynthesis of polyunsaturated fatty acids (PUFAs). However, the enzyme exhibits a significant preference towards different fatty acid substrates. To examine the molecular mechanism of its substrate specificity, a series of site-directed mutants were constr...

journal_title：Applied microbiology and biotechnology

authors： Rong C,Chen H,Wang M,Gu Z,Zhao J,Zhang H,Chen W,Chen YQ

更新日期：2018-11-01 00:00:00

abstract：:The fungal conversions of sulfur-containing heterocyclic compounds were investigated using the lignin-degrading basidiomycete Coriolus versicolor. The fungus metabolized a series of sulfur compounds--25 structurally related thiophene derivatives--via several different pathways. Under primary metabolic conditions, C. v...

journal_title：Applied microbiology and biotechnology

authors： Ichinose H,Nakamizo M,Wariishi H,Tanaka H

更新日期：2002-03-01 00:00:00

abstract：:This study focused on the use the thermophilic anaerobic granulae in the start-up of 70 degrees C acetate-fed upflow anaerobic sludge-blanket (UASB) reactors and the kinetics of granulae grown at 70 degrees C. In the UASB reactors, chemical oxygen demand removal commenced within 48 h of the start-up. The maximum reduc...

journal_title：Applied microbiology and biotechnology

authors： Lepistö R,Rintala JA

更新日期：1995-11-01 00:00:00

abstract：:Inorganic sulfate is the preferred sulfur source for the growth of most microorganisms but, in its absence, many organosulfur compounds can be degraded microbially to provide sulfur. Desulfurization of dibenzothiophene (DBT) by Rhodococcus sp. and of aromatic sulfonates by Pseudomonas sp. has considerable biotechnolog...

journal_title：Applied microbiology and biotechnology

authors： Kertesz MA,Wietek C

更新日期：2001-11-01 00:00:00

abstract：:Staphylococcus aureus is a pathogen responsible for severe community- and nosocomially acquired infections. To fight pathogen intrusion, the innate immune system uses a plethora of weapons, with the generation of oxidative and nitrosative stresses among the most efficient. In this work, the S. aureus genome-wide trans...

journal_title：Applied microbiology and biotechnology

authors： Nobre LS,Saraiva LM

更新日期：2013-03-01 00:00:00

abstract：:A cDNA fragment encoding the A catalytic domain of the Neocallimastix frontalis endoxylanase XYN3 was amplified and cloned by the polymerase chain reaction technique. The xyn3A DNA fragment was inserted between the Saccharomyces cerevisiae phosphoglycerate kinase gene promoter and terminator sequences on a multicopy e...

journal_title：Applied microbiology and biotechnology

authors： Durand R,Rascle C,Fèvre M

更新日期：1999-08-01 00:00:00

abstract：:A new pmoA gene-based PCR primer set was designed for detection of nitrite-dependent anaerobic oxidation of methane (n-damo) bacteria from four different ecosystems, namely rice paddy soil, freshwater reservoir, reed bed, and sludge from wastewater treatment plant. This primer set showed high specificity and efficienc...

journal_title：Applied microbiology and biotechnology

authors： Han P,Gu JD

更新日期：2013-12-01 00:00:00

abstract：:Monascus pigments (Mps) are a group of azaphilonic secondary metabolites produced by Monascus spp. via a polyketide pathway. A mutant deleted an about 30 kb region of Mps gene cluster from Monascus ruber M7 was isolated previously, which produces a high amount of a light yellow pigment. The current study revealed that...

journal_title：Applied microbiology and biotechnology

authors： Liu J,Zhou Y,Yi T,Zhao M,Xie N,Lei M,Liu Q,Shao Y,Chen F

更新日期：2016-08-01 00:00:00

abstract：:The manganese superoxide dismutase gene (sodM) is very highly expressed in Aspergillus oryzae. To elucidate the basis for this high-level expression, deletion analysis of the promoter was undertaken using beta-glucuronidase (GUS) as a reporter. Deletion of a 63-bp sequence from -200 to -138 in the 1,038-bp sodM promot...

journal_title：Applied microbiology and biotechnology

authors： Hisada H,Sano M,Ishida H,Hata Y,Abe Y,Machida M

更新日期：2006-10-01 00:00:00

abstract：:Iron reduction mediated by Fe(III)-reducing bacteria (FeRB) occurs in aqueous environments and plays an essential role in removing contaminates in polluted freshwater lakes. Two model FeRB species, Shewanella and Geobacter, have been intensively studied because of their functions in bioremediation, iron reduction, and...

journal_title：Applied microbiology and biotechnology

authors： Fan YY,Li BB,Yang ZC,Cheng YY,Liu DF,Yu HQ

更新日期：2018-12-01 00:00:00

abstract：:A maltotriose-producing α-amylase, AmyA, from a newly isolated bacterial strain Microbulbifer thermotolerans DAU221 was purified and characterized in the heterologous host, Escherichia coli, using the pCold I vector. The amyA gene encoded a 761-residue protein composed of a 33 amino acid secretion signal peptide. The ...

journal_title：Applied microbiology and biotechnology

authors： Lee YS,Park DJ,Choi YL

更新日期：2015-05-01 00:00:00

abstract：:Many microorganisms, growing on aging flue-cured tobacco leaves, play a part in its fermentation process. These microflora were identified and described by culture-dependent methods earlier. In this study we report the identity of the microflora growing on the tobacco leaf surface by employing culture-independent meth...

journal_title：Applied microbiology and biotechnology

authors： Zhao M,Wang B,Li F,Qiu L,Li F,Wang S,Cui J

更新日期：2007-01-01 00:00:00

abstract：:Under alternating aerobic/anaerobic conditions and without additional carbon sources, a bacterial consortium consisting initially of 18 bacterial strains was obtained in a sequence batch reactor. The phosphorus removal capability could only be maintained using sterile filtrate of activated sludge as medium. The additi...

journal_title：Applied microbiology and biotechnology

authors： Hollender J,Dreyer U,Kornberger L,Kämpfer P,Dott W

更新日期：2002-01-01 00:00:00

abstract：:The redox-sensing transcriptional repressor Rex (Rex) displayed diverse functions in different microbial species. Nowadays, only part function of rex has been verified in vitro and alcohol dehydrogenase gene (adhE) as the target of Rex has been widely reported. In this study, rex was knocked out in Thermoanaerobacteri...

journal_title：Applied microbiology and biotechnology

authors： Qu C,Chen L,Li Y,Fu H,Wang J

更新日期：2020-06-01 00:00:00

abstract：:Escherichia coli and Lactobacillus plantarum were subjected to final water potentials of -5.6 MPa and -11.5 MPa with three solutes: glycerol, sorbitol and NaCl. The water potential decrease was realized either rapidly (osmotic shock) or slowly (20 min) and a difference in cell viability between these conditions was on...

journal_title：Applied microbiology and biotechnology

authors： Poirier I,Maréchal PA,Evrard C,Gervais P

更新日期：1998-12-01 00:00:00

abstract：:Repetitious gene cassettes that encode the consensus decapeptide repeat of Mytilus edulis bioadhesive protein were designed, constructed, and expressed in Escherichia coli. The bioadhesive precursor (BP) with a relative molecular mass of 25,000 was expressed from one 600-bp gene at levels approaching 60% of total cell...

journal_title：Applied microbiology and biotechnology

authors： Salerno AJ,Goldberg I

更新日期：1993-05-01 00:00:00

abstract：:6-Oxopiperidine-2-carboxylic acid (OCA; cyclic alpha-aminoadipic acid) reverses the L-lysine inhibition of penicillin G production by Penicillium chrysogenum PQ-96. The reaction probably depends on the recovery of L-alpha-aminoadipic acid for penicillin G production from OCA. ...

journal_title：Applied microbiology and biotechnology

authors： Kurzatkowski W,Kurzatkowski JD,Filipek J,Solecka J,Kuryłowicz W

更新日期：1990-12-01 00:00:00