Abstract：
:Many microorganisms, growing on aging flue-cured tobacco leaves, play a part in its fermentation process. These microflora were identified and described by culture-dependent methods earlier. In this study we report the identity of the microflora growing on the tobacco leaf surface by employing culture-independent methods. We have amplified microbial 16S rDNA sequences directly from the leaf surface and used denaturing gradient gel electrophoresis (DGGE) to identify bacterial community on the tobacco leaves. Our culture-independent methods for the study of microbial community on tobacco leaves showed that microbial community structures on leaves of variety Zhongyan 100, NC89 and Zhongyan 101 were similar between 0 and 6 months aging, and between 9 and 12 months aging, while the similarity is low between 0 and 6, and between 9 and 12 months aging, respectively. There were certain similarities of bacterial communities (similarity up to 63%) among the three tobacco varieties for 0 to 6 months aging. Five dominant 16S rDNA DGGE bands A, B, C, D and E were isolated, cloned, and sequenced. They were most similar to two cultured microbial species Bacteriovorax sp. EPC3, Bacillus megaterium, and three uncultured microbial species, respectively.
journal_name
Appl Microbiol Biotechnoljournal_title
Applied microbiology and biotechnologyauthors
Zhao M,Wang B,Li F,Qiu L,Li F,Wang S,Cui Jdoi
10.1007/s00253-006-0625-xsubject
Has Abstractpub_date
2007-01-01 00:00:00pages
1435-40issue
6eissn
0175-7598issn
1432-0614journal_volume
73pub_type
杂志文章abstract：:In natural settings, anaerobic digestion can take place in a wide temperature range, but industrial digesters are usually operated under either mesophilic (~35 °C) or thermophilic (~55 °C) conditions. The ability of anaerobic digestion microbiota to switch from one operating temperature to the other remains poorly doc...
journal_title：Applied microbiology and biotechnology
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更新日期：2016-02-01 00:00:00
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journal_title：Applied microbiology and biotechnology
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更新日期：2001-09-01 00:00:00
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更新日期：2008-01-01 00:00:00
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pub_type： 临床试验,杂志文章,多中心研究
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更新日期：1991-03-01 00:00:00
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更新日期：2007-02-01 00:00:00
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pub_type： 杂志文章,评审
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