当前位置： SCI文献检索 > APPLIED MICROBIOLOGY AND BIOTECHNOLOGY期刊下所有文献 > Production of selenomethionine-labelled proteins using simplified culture conditions and generally applicable host/vector systems.

Production of selenomethionine-labelled proteins using simplified culture conditions and generally applicable host/vector systems.

Abstract：

:The amino acid analogue selenomethionine (SeMet) is shown to be efficiently incorporated into recombinant proteins expressed in Escherichia coli grown in a simple minimal medium without the addition of synthetic amino acids. Furthermore, satisfactory SeMet incorporation is obtained with a methionine-prototrophic strain transformed with commonly used vector systems. As examples, purified tryparedoxin 1 from Crithidia fasciculata, alkylhydroperoxide reductase (AhpC) from Mycobacterium marinum and the 16-kDa antigen from M. tuberculosis are shown to be efficiently labelled with SeMet, using the culture conditions and the host/vector systems described here. Enzymatic analysis reveals no differences between native and SeMet-labelled tryparedoxin 1 enzyme. Both proteins yield crystals under similar conditions. The culture conditions and host vector systems described greatly facilitate selenium-labelling of proteins for 3-D structure determination.

journal_name

Appl Microbiol Biotechnol

journal_title

Applied microbiology and biotechnology

authors

Guerrero SA,Hecht HJ,Hofmann B,Biebl H,Singh M

doi

10.1007/s002530100690

subject

Has Abstract

pub_date

2001-09-01 00:00:00

pages

718-23

issue

5-6

eissn

0175-7598

issn

1432-0614

journal_volume

56

pub_type

杂志文章

相关文献

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY文献大全
  • Improvement of levan production in Bacillus amyloliquefaciens through metabolic optimization of regulatory elements.

    abstract：:Levan is a functional homopolymer of fructose with considerable applications in food, pharmaceutical and cosmetic industries. To improve the levan production in Bacillus amyloliquefaciens, the regulatory elements of sacB (encoding levansucrase) expression and levansucrase secretion were optimized. Four heterologous pr...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-017-8171-2

    authors： Gu Y,Zheng J,Feng J,Cao M,Gao W,Quan Y,Dang Y,Wang Y,Wang S,Song C

    更新日期：2017-05-01 00:00:00

  • A new mathematical model for nitrogen gas production with special emphasis on the role of attached growth media in anammox hybrid reactor.

    abstract：:The present study emphasised on the development of new mathematical models based on mass balance and stoichiometry of nitrogen removal in anammox hybrid reactor (AHR). The performance of AHR at varying hydraulic retention times (HRTs) and nitrogen loading rates (NLRs) revealed that nitrogen removal efficiency (NRE) in...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-015-6793-9

    authors： Tomar S,Gupta SK

    更新日期：2015-11-01 00:00:00

  • Enzymatic liquefaction of agarose above the sol-gel transition temperature using a thermostable endo-type β-agarase, Aga16B.

    abstract：:The main carbohydrate of red macroalgae is agarose, a heterogeneous polysaccharide composed of D-galactose and 3,6-anhydro-L-galactose. When saccharifying agarose by enzymes, the unique physical properties of agarose, namely the sol-gel transition and the near-insolubility of agarose in water, limit the accessibility ...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-016-7831-y

    authors： Kim JH,Yun EJ,Seo N,Yu S,Kim DH,Cho KM,An HJ,Kim JH,Choi IG,Kim KH

    更新日期：2017-02-01 00:00:00

  • Comparative analysis of genetic diversity and expression of amoA in wastewater treatment processes.

    abstract：:The genetic diversity and expression of amoA of autotrophic ammonia oxidizers in wastewater treatment processes were investigated by RT-PCR and denaturing gradient gel electrophoresis (DGGE) in order to identify active components of ammonia-oxidizer populations in a such processes. Ammonia oxidizers, evidenced by the ...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-004-1558-x

    authors： Ebie Y,Noda N,Miura H,Matsumura M,Tsuneda S,Hirata A,Inamori Y

    更新日期：2004-06-01 00:00:00

  • Dominance of Prevotella and low abundance of classical ruminal bacterial species in the bovine rumen revealed by relative quantification real-time PCR.

    abstract：:Relative quantification real-time PCR was used to quantify several bacterial species in ruminal samples from two lactating cows, each sampled 3 h after feeding on two successive days. Abundance of each target taxon was calculated as a fraction of the total 16S rRNA gene copies in the samples, using taxon-specific and ...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-006-0802-y

    authors： Stevenson DM,Weimer PJ

    更新日期：2007-05-01 00:00:00

  • Use of real time PCR to determine population profiles of individual species of lactic acid bacteria in alfalfa silage and stored corn stover.

    abstract：:Real-time polymerase chain reaction (RT-PCR) was used to quantify seven species of lactic acid bacteria (LAB) in alfalfa silage prepared in the presence or absence of four commercial inoculants and in uninoculated corn stover harvested and stored under a variety of field conditions. Species-specific PCR primers were d...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-005-0170-z

    authors： Stevenson DM,Muck RE,Shinners KJ,Weimer PJ

    更新日期：2006-07-01 00:00:00

  • Development of chitosan-coated agar-gelatin particles for probiotic delivery and targeted release in the gastrointestinal tract.

    abstract：:This study reports the development of a novel and simple formulation for probiotic delivery using chitosan-coated agar-gelatin gel particles. This methodology involves the production of agar-gelatin particles by thermally treating a mixture of agar and gelatin solutions at high temperatures (121 °C) and subsequently c...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-020-10632-w

    authors： Albadran HA,Monteagudo-Mera A,Khutoryanskiy VV,Charalampopoulos D

    更新日期：2020-07-01 00:00:00

  • Impact of plasmid architecture on stability and yEGFP3 reporter gene expression in a set of isomeric multicopy vectors in yeast.

    abstract：:Multicopy episomal plasmids in yeast, used whenever elevated levels of foreign or homologous gene expression are necessary, are known to be less stable compared to the endogenous 2-μm plasmid they are based on, at least without selective pressure. Considering that rich medium favors growth rate and, simultaneously, is...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-017-8558-0

    authors： Hohnholz R,Pohlmann KJ,Achstetter T

    更新日期：2017-12-01 00:00:00

  • Vinyl ketone reduction by three distinct Gluconobacter oxydans 621H enzymes.

    abstract：:Three cytosolic NADPH-dependent flavin-associated proteins (Gox2107, Gox0502, and Gox2684) from Gluconobacter oxydans 621H were overproduced in Escherichia coli, and the recombinant enzymes were purified and characterized. Apparent native molecular masses of 65.2, 78.2, and 78.4 kDa were observed for Gox2107, Gox0502,...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-008-1600-5

    authors： Schweiger P,Gross H,Wesener S,Deppenmeier U

    更新日期：2008-10-01 00:00:00

  • Increased intracellular calcium level and impaired nutrient absorption are important pathogenicity traits in the chicken intestinal epithelium during Campylobacter jejuni colonization.

    abstract：:Although a high number of chickens carry Campylobacter jejuni, the mechanistic action of colonization in the intestine is still poorly understood. The current study was therefore designed to investigate the effects of C. jejuni on glucose uptake, amino acids availability in digesta, and intracellular calcium [Ca(2+)]i...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-015-6543-z

    authors： Awad WA,Smorodchenko A,Hess C,Aschenbach JR,Molnár A,Dublecz K,Khayal B,Pohl EE,Hess M

    更新日期：2015-08-01 00:00:00

  • Microbial decolourisation and degradation of textile dyes.

    abstract：:Dyes and dyestuffs find use in a wide range of industries but are of primary importance to textile manufacturing. Wastewater from the textile industry can contain a variety of polluting substances including dyes. Increasingly, environmental legislation is being imposed to control the release of dyes, in particular azo...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章,评审

    doi：10.1007/s002530000587

    authors： McMullan G,Meehan C,Conneely A,Kirby N,Robinson T,Nigam P,Banat IM,Marchant R,Smyth WF

    更新日期：2001-07-01 00:00:00

  • The mechanism of bacterial indigo reduction.

    abstract：:The reduction of water-insoluble indigo by the recently isolated moderate thermophile, Clostridium isatidis, has been studied with the aim of developing a sustainable technology for industrial indigo reduction. The ability to reduce indigo was not shared with C. aurantibutyricum, C. celatum and C. papyrosolvens, but C...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-004-1839-4

    authors： Nicholson SK,John P

    更新日期：2005-07-01 00:00:00

  • Arabinoxylan and mono- and dimeric ferulic acid release from brewer's grain and wheat bran by feruloyl esterases and glycosyl hydrolases from Humicola insolens.

    abstract：:An enzyme preparation from the thermophilic fungus Humicola insolens, Ultraflo L, was able to solubilise more than half of the biomass of brewer's grain and wheat bran, two agro-industrial co-products. While almost all of the ferulic acid was released in the free form, the majority of diferulates were released still a...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-003-1520-3

    authors： Faulds CB,Mandalari G,LoCurto R,Bisignano G,Waldron KW

    更新日期：2004-06-01 00:00:00

  • Phosphate starvation controls lactose metabolism to produce recombinant protein in Escherichia coli.

    abstract：:Phosphate is one of the major constituents in growth media. It closely regulates central carbon and energy metabolism. Biochemical reactions in central carbon metabolism are known to be regulated by phosphorylation and dephosphorylation of enzymes. Phosphate scarcity can limit microbial productivity. However, microorg...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-020-10935-y

    authors： Pandi K,Chauhan AS,Khan WH,Rathore AS

    更新日期：2020-11-01 00:00:00

  • Uniform stable-isotope labeling in mammalian cells: formulation of a cost-effective culture medium.

    abstract：:Uniform stable-isotope labeling of mammalian cells is achieved via a novel formulation of a serum-free cell culture medium that is based on stable-isotope-labeled autolysates and lipid extracts of various microbiological origin. Yeast autolysates allow complete replacement of individual amino acids and organic acids i...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-010-2896-5

    authors： Egorova-Zachernyuk TA,Bosman GJ,Degrip WJ

    更新日期：2011-01-01 00:00:00

  • Release of ferulic acid from wheat bran by a ferulic acid esterase (FAE-III) from Aspergillus niger.

    abstract：:Ferulic acid was efficiently released from a wheat bran preparation by a ferulic acid esterase from Aspergillus niger (FAE-III) when incubated together with a Trichoderma viride xylanase (a maximum of 95% total ferulic acid released after 5 h incubation). FAE-III by itself could release ferulic acid but at a level alm...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/BF00166929

    authors： Faulds CB,Williamson G

    更新日期：1995-11-01 00:00:00

  • Microbial production of poly(hydroxybutyrate) from C₁ carbon sources.

    abstract：:Polyhydroxybutyrate (PHB) is an attractive substitute for petrochemical plastic due to its similar properties, biocompatibility, and biodegradability. The cost of scaled-up PHB production inhibits its widespread usage. Intensive researches are growing to reduce costs and improve thermomechanical, physical, and process...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章,评审

    doi：10.1007/s00253-012-4649-0

    authors： Khosravi-Darani K,Mokhtari ZB,Amai T,Tanaka K

    更新日期：2013-02-01 00:00:00

  • Overview of thermostable DNA polymerases for classical PCR applications: from molecular and biochemical fundamentals to commercial systems.

    abstract：:During the genomics era, the use of thermostable DNA polymerases increased greatly. Many were identified and described-mainly of the genera Thermus, Thermococcus and Pyrococcus. Each polymerase has different features, resulting from origin and genetic modification. However, the rational choice of the adequate polymera...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章,评审

    doi：10.1007/s00253-013-5290-2

    authors： Terpe K

    更新日期：2013-12-01 00:00:00

  • Laccase applications in biofuels production: current status and future prospects.

    abstract：:The desire to reduce dependence on the ever diminishing fossil fuel reserves coupled with the impetus towards green energy has seen increased research in biofuels as alternative sources of energy. Lignocellulose materials are one of the most promising feedstocks for advanced biofuels production. However, their utilisa...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章,评审

    doi：10.1007/s00253-014-5810-8

    authors： Kudanga T,Le Roes-Hill M

    更新日期：2014-08-01 00:00:00

  • Microbial aspartic proteases: current and potential applications in industry.

    abstract：:Aspartic proteases are a relatively small group of proteolytic enzymes that are active in acidic environments and are found across all forms of life. Certain microorganisms secrete such proteases as virulence agents and/or in order to break down proteins thereby liberating assimilable sources of nitrogen. Some of the ...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章,评审

    doi：10.1007/s00253-014-6035-6

    authors： Theron LW,Divol B

    更新日期：2014-11-01 00:00:00

  • Biomethane production and microbial community response according to influent concentration of molasses wastewater in a UASB reactor.

    abstract：:This study aimed to investigate the interaction between methane production performance and active microbial community dynamics at different loading rates by increasing influent substrate concentration. The model system was an upflow anaerobic sludge blanket (UASB) reactor using molasses wastewater. The active microbia...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-016-7314-1

    authors： Yun J,Lee SD,Cho KS

    更新日期：2016-05-01 00:00:00

  • Molecular cloning and characterization of a GH11 endoxylanase from Chaetomium globosum, and its use in enzymatic pretreatment of biomass.

    abstract：:An endo-1,4-β-xylanase gene, xylcg, was cloned from Chaetomium globosum and successfully expressed in Escherichia coli. The complete gene of 675 bp was amplified, cloned into the pET 28(a) vector, and expressed. The optimal conditions for the highest activity of the purified recombinant XylCg were observed at a temper...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-012-4577-z

    authors： Singh RK,Tiwari MK,Kim D,Kang YC,Ramachandran P,Lee JK

    更新日期：2013-08-01 00:00:00

  • Diversity and catalytic potential of PAH-specific ring-hydroxylating dioxygenases from a hydrocarbon-contaminated soil.

    abstract：:Ring-hydroxylating dioxygenases (RHDs) catalyze the initial oxidation step of a range of aromatic hydrocarbons including polycyclic aromatic hydrocarbons (PAHs). As such, they play a key role in the bacterial degradation of these pollutants in soil. Several polymerase chain reaction (PCR)-based methods have been imple...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-012-4335-2

    authors： Martin F,Malagnoux L,Violet F,Jakoncic J,Jouanneau Y

    更新日期：2013-06-01 00:00:00

  • Analysis of acyl CoA ester intermediates of the mevalonate pathway in Saccharomyces cerevisiae.

    abstract：:The mevalonate pathway plays an important role in providing the cell with a number of essential precursors for the synthesis of biomass constituents. With respect to their chemical structure, the metabolites of this pathway can be divided into two groups: acyl esters [acetoacetyl CoA, acetyl CoA, hydroxymethylglutaryl...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-004-1697-0

    authors： Seker T,Møller K,Nielsen J

    更新日期：2005-04-01 00:00:00

  • Cell-targeting aptamers act as intracellular delivery vehicles.

    abstract：:Aptamers are single-stranded nucleic acids or peptides identified from a randomized combinatorial library through specific interaction with the target of interest. Targets can be of any size, from small molecules to whole cells, attesting to the versatility of aptamers for binding a wide range of targets. Aptamers sho...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章,评审

    doi：10.1007/s00253-016-7686-2

    authors： Gopinath SC,Lakshmipriya T,Chen Y,Arshad MK,Kerishnan JP,Ruslinda AR,Al-Douri Y,Voon CH,Hashim U

    更新日期：2016-08-01 00:00:00

  • Heterologous overproduction of β-fructofuranosidase from yeast Xanthophyllomyces dendrorhous, an enzyme producing prebiotic sugars.

    abstract：:The β-fructofuranosidase Xd-INV from the yeast Xanthophyllomyces dendrorhous is the largest microbial enzyme producing neo-fructooligosaccharides (neo-FOS) known to date. It mainly synthesizes neokestose and neonystose, oligosaccharides with potentially improved prebiotic properties. The Xd-INV gene comprises an open ...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-014-6145-1

    authors： Gimeno-Pérez M,Linde D,Fernández-Arrojo L,Plou FJ,Fernández-Lobato M

    更新日期：2015-04-01 00:00:00

  • Growth kinetics of animal cells immobilized within porous support particles in a perfusion culture.

    abstract：:The growth kinetics of anchorage-independent animal cells [mouse myeloma MPC-11 (ATCC CCL 167)] immobilized within porous polyvinyl formal resin biomass support particles (BSPs; 3 x 3 x 3 mm cubes; mean pore diameter, 60 microns) was analyzed by measuring intracellular and extracellular lactate dehydrogenase (LDH) act...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/BF00173916

    authors： Yamaji H,Fukuda H

    更新日期：1994-12-01 00:00:00

  • Occurrence and function of enzymes for lignocellulose degradation in commercial Agaricus bisporus cultivation.

    abstract：:The white button mushroom Agaricus bisporus is economically the most important commercially produced edible fungus. It is grown on carbon- and nitrogen-rich substrates, such as composted cereal straw and animal manure. The commercial mushroom production process is usually performed in buildings or tunnels under highly...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章,评审

    doi：10.1007/s00253-017-8294-5

    authors： Kabel MA,Jurak E,Mäkelä MR,de Vries RP

    更新日期：2017-06-01 00:00:00

  • Influence of stringent and relaxed response on excretion of recombinant proteins and fatty acid composition in Escherichia coli.

    abstract：:In contrast to stringent (relA+) cells of Escherichia coli, relaxed (relA) cells excreted recombinant proteins (beta-lactamase, interferon alpha 1) into the culture medium during amino acid limitation. Comparative analyses of overall fatty acid composition in relA+ cells and relA cells were performed and revealed that...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/BF00170628

    authors： Gitter B,Diefenbach R,Keweloh H,Riesenberg D

    更新日期：1995-04-01 00:00:00

  • Protein engineering of toluene ortho-monooxygenase of Burkholderia cepacia G4 for regiospecific hydroxylation of indole to form various indigoid compounds.

    abstract：:Previous work showed that random mutagenesis produced a mutant of toluene ortho-monooxygenase (TOM) of Burkholderia cepacia G4 containing the V106A substitution in the hydroxylase alpha-subunit (TomA3) that changed the color of the cell suspension from wild-type brown to green in rich medium. Here, DNA shuffling was u...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-004-1698-z

    authors： Rui L,Reardon KF,Wood TK

    更新日期：2005-01-01 00:00:00