Microbial aspartic proteases: current and potential applications in industry.

Abstract:

:Aspartic proteases are a relatively small group of proteolytic enzymes that are active in acidic environments and are found across all forms of life. Certain microorganisms secrete such proteases as virulence agents and/or in order to break down proteins thereby liberating assimilable sources of nitrogen. Some of the earlier applications of these proteolytic enzymes are found in the manufacturing of cheese where they are used as milk-clotting agents. Over the last decade, they have received tremendous research interest because of their involvement in human diseases. Furthermore, there has also been a growing interest on these enzymes for their applications in several other industries. Recent research suggests in particular that they could be used in the wine industry to prevent the formation of protein haze while preserving the wines' organoleptic properties. In this mini-review, the properties and mechanisms of action of aspartic proteases are summarized. Thereafter, a brief overview of the industrial applications of this specific class of proteases is provided. The use of aspartic proteases as alternatives to clarifying agents in various beverage industries is mentioned, and the potential applications in the wine industry are thoroughly discussed.

authors

Theron LW,Divol B

doi

10.1007/s00253-014-6035-6

subject

Has Abstract

pub_date

2014-11-01 00:00:00

pages

8853-68

issue

21

eissn

0175-7598

issn

1432-0614

journal_volume

98

pub_type

杂志文章,评审
  • Stereospecific microbial production of isoflavanones from isoflavones and isoflavone glucosides.

    abstract::A Gram-negative anaerobic microorganism, MRG-1, isolated from human intestine showed high activities of deglycosylation and reduction of daidzin, based on rapid TLC analysis. A rod-shaped strain MRG-1 was identified as a new species showing 91.0% homology to Coprobacillus species, based on 16S rRNA sequence analysis. ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-011-3310-7

    authors: Park HY,Kim M,Han J

    更新日期:2011-08-01 00:00:00

  • Use of 2-deoxyglucose in liquid media for the selection of mutant strains of Penicillium echinulatum producing increased cellulase and beta-glucosidase activities.

    abstract::Mutagenesis and selection were applied to a strain of Penicillium echinulatum by treating conidia with hydrogen peroxide or 1,2,7,8-diepoxyoctane and then by incubating the conidia for 48 h in broth containing microcrystalline cellulose washed in 0.5% (w/v) aqueous 2-deoxyglucose before plating them onto cellulose aga...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-005-0122-7

    authors: Dillon AJ,Zorgi C,Camassola M,Henriques JA

    更新日期:2006-05-01 00:00:00

  • Biophotography: concepts, applications and perspectives.

    abstract::Synthetic biology aims at manipulating biological systems by rationally designed and genetically introduced components. Efforts in photoactuator engineering resulted in microorganisms reacting to extracellular light-cues with various cellular responses. Some of them lead to the formation of macroscopically observable ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-016-7384-0

    authors: Renicke C,Taxis C

    更新日期:2016-04-01 00:00:00

  • Transcriptional remodeling in response to transfer upon carbon-limited or metformin-supplemented media in S. cerevisiae and its effect on chronological life span.

    abstract::One of the factors affecting chronological life span (CLS) in budding yeast is nutrient, especially carbon limitation. Aside from metabolites in the growth medium such as glucose, amino acids, and acetic acid, many pharmaceuticals have also been proven to alter CLS. Besides their impact on life span, these drugs are a...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-015-6728-5

    authors: Borklu-Yucel E,Eraslan S,Ulgen KO

    更新日期:2015-08-01 00:00:00

  • The mechanism of bacterial indigo reduction.

    abstract::The reduction of water-insoluble indigo by the recently isolated moderate thermophile, Clostridium isatidis, has been studied with the aim of developing a sustainable technology for industrial indigo reduction. The ability to reduce indigo was not shared with C. aurantibutyricum, C. celatum and C. papyrosolvens, but C...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-004-1839-4

    authors: Nicholson SK,John P

    更新日期:2005-07-01 00:00:00

  • C16-Fengycin A affect the growth of Candida albicans by destroying its cell wall and accumulating reactive oxygen species.

    abstract::Candida albicans is the most common clinical pathogenic fungus, which is highly susceptible to immunodeficiency. Development of novel antifungal agents has become a growing trend in the treatment of Candida infections. C16-Fengycin A, a lipopeptide isolated from Bacillus amyloliquefaciens fmb60 showed significant fung...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-019-10117-5

    authors: Liu Y,Lu J,Sun J,Zhu X,Zhou L,Lu Z,Lu Y

    更新日期:2019-11-01 00:00:00

  • The redox-sensing transcriptional repressor Rex is important for regulating the products distribution in Thermoanaerobacterium aotearoense SCUT27.

    abstract::The redox-sensing transcriptional repressor Rex (Rex) displayed diverse functions in different microbial species. Nowadays, only part function of rex has been verified in vitro and alcohol dehydrogenase gene (adhE) as the target of Rex has been widely reported. In this study, rex was knocked out in Thermoanaerobacteri...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-020-10554-7

    authors: Qu C,Chen L,Li Y,Fu H,Wang J

    更新日期:2020-06-01 00:00:00

  • Optimization of the extracellular production of a bacterial phytase with Escherichia coli by using different fed-batch fermentation strategies.

    abstract::The extracellular production of Escherichia coli phytase was studied in fed-batch fermentations. Two different feeding strategies were compared: control by keeping the glucose concentration constant, and control by keeping a low constant oxygen level in the medium. For the feeding control based on glucose concentratio...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-003-1229-3

    authors: Kleist S,Miksch G,Hitzmann B,Arndt M,Friehs K,Flaschel E

    更新日期:2003-06-01 00:00:00

  • Regulation of Aspergillus genes encoding plant cell wall polysaccharide-degrading enzymes; relevance for industrial production.

    abstract::The genus Aspergillus is widely used for the production of plant cell wall polysaccharide-degrading enzymes. The range of enzymes purified from these fungi covers nearly every function required for the complete degradation of cellulose, xyloglucan, xylan, galacto(gluco)mannan and pectin. This paper describes the Asper...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-002-1171-9

    authors: de Vries RP

    更新日期:2003-03-01 00:00:00

  • Biosynthesis of isocitric acid in repeated-batch culture and testing of its stress-protective activity.

    abstract::Biosynthesis of Ds(+)-threo-isocitric acid from ethanol in the Yarrowia lipolytica batch and repeated-batch cultures was studied. Repeated-batch cultivation was found to provide for a good biosynthetic efficiency of the producer for as long as 748 h, probably due to maintenance of high activities of enzymes involved i...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-019-09729-8

    authors: Morgunov IG,Kamzolova SV,Karpukhina OV,Bokieva SB,Inozemtsev AN

    更新日期:2019-04-01 00:00:00

  • Waste valorization by biotechnological conversion into added value products.

    abstract::Fossil fuel reserves depletion, global warming, unrelenting population growth, and costly and problematic waste recycling call for renewable resources of energy and consumer products. As an alternative to the 100 % oil economy, production processes based on biomass can be developed. Huge amounts of lignocellulosic was...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-013-5014-7

    authors: Liguori R,Amore A,Faraco V

    更新日期:2013-07-01 00:00:00

  • The NT11, a novel fusion tag for enhancing protein expression in Escherichia coli.

    abstract::The Escherichia coli (E. coli) expression system has been widely used to produce recombinant proteins. However, in some heterologous expressions, there are still difficulties in large-scale production. The use of fusion partners is one of the strategies for improving the expression levels of proteins in E. coli host. ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-018-09595-w

    authors: Nguyen TKM,Ki MR,Son RG,Pack SP

    更新日期:2019-03-01 00:00:00

  • Medicinal mushroom modulators of molecular targets as cancer therapeutics.

    abstract::Empirical approaches to discover anticancer drugs and cancer treatments have made limited progress in the past several decades in finding a cure for cancer. The expanded knowledge of the molecular basis of tumorigenesis and metastasis, together with the inherently vast structural diversity of natural compounds found i...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-004-1787-z

    authors: Zaidman BZ,Yassin M,Mahajna J,Wasser SP

    更新日期:2005-06-01 00:00:00

  • L-valine, an antialgal amino acid from Streptomyces jiujiangensis JXJ 0074(T).

    abstract::An antialgal compound was isolated from the cultured broth of Streptomyces jiujiangensis JXJ 0074(T) by using bioassay methods. Based on the data of (1)H-NMR, (13)C-NMR, ESI-MS, and thin layer chromatography, the active compound was identified as L-valine, which showed antialgal activity mainly against Microcystis. L-...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-015-7150-8

    authors: Zhang BH,Chen W,Li HQ,Yang JY,Zha DM,Duan YQ,N Hozzein W,Xiao M,Gao R,Li WJ

    更新日期:2016-05-01 00:00:00

  • The three tricarboxylate synthase activities of Corynebacterium glutamicum and increase of L-lysine synthesis.

    abstract::Corynebacterium glutamicum owns a citrate synthase and two methylcitrate synthases. Characterization of the isolated enzymes showed that the two methylcitrate synthases have comparable catalytic efficiency, k (cat)/K (m), as the citrate synthase with acetyl-CoA as substrate, although these enzymes are only synthesized...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-007-1105-7

    authors: Radmacher E,Eggeling L

    更新日期:2007-09-01 00:00:00

  • A novel self-cleavable tag Zbasic-∆I-CM and its application in the soluble expression of recombinant human interleukin-15 in Escherichia coli.

    abstract::Soluble expression of recombinant therapeutic proteins in Escherichia coli (E. coli) has been a challenging task in biopharmaceutical development. In this study, a novel self-cleavable tag Zbasic-intein has been constructed for the soluble expression and purification of a recombinant cytokine, human interleukin-15 (IL...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-016-7848-2

    authors: Shi S,Chen H,Jiang H,Xie Y,Zhang L,Li N,Zhu C,Chen J,Luo H,Wang J,Feng L,Lu H,Zhu J

    更新日期:2017-02-01 00:00:00

  • Response of rhizosphere microbial community structure and diversity to heavy metal co-pollution in arable soil.

    abstract::Due to the emerging environmental issues related to heavy metals, concern about the soil quality of farming lands near manufacturing district is increasing. Investigating the function of soil microorganisms exposed to long-term heavy metal contamination is meaningful and important for agricultural soil utilization. Th...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-015-6662-6

    authors: Deng L,Zeng G,Fan C,Lu L,Chen X,Chen M,Wu H,He X,He Y

    更新日期:2015-10-01 00:00:00

  • Expression of a synthetic protein-based polymer (elastomer) gene in Aspergillus nidulans.

    abstract::A gene for a synthetic protein-based polymer, G-(VPGVG)119-VPGV, coding for the EG-120mer (elastomer), was cloned into a fungal expression vector to allow constitutive expression of the polymer controlled by the gpdA (glyceraldehyde-3-phosphate dehydrogenase) promoter sequence of Aspergillus nidulans. Stable transform...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s002530050942

    authors: Herzog RW,Singh NK,Urry DW,Daniell H

    更新日期:1997-04-01 00:00:00

  • Screening of tetrachlorodibenzo- p-dioxin-degrading fungi capable of producing extracellular peroxidases under various conditions.

    abstract::Forty-six pulp-bleaching fungi were screened for production of key enzymes for conversion of polychlorinated dibenzo-p-dioxins--lignin peroxidase (LiP), manganese peroxidase (MnP), and manganese-independent peroxidase (MiP)--under various conditions that would allow their utilization in the environment. Of 38 MnP-prod...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-003-1356-x

    authors: Manji S,Ishihara A

    更新日期:2004-01-01 00:00:00

  • Modelling and parameter identification for batch fermentations with Streptomyces tendae under phosphate limitation.

    abstract::A simple structured model for the dynamics of phosphate-limited batch fermentations with Streptomyces tendae is presented. The model describes the influence of intracellular phosphate storage upon the growth behav our of the culture. The development of the model takes into account the possible internal regulatory proc...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00172717

    authors: Mundry C,Kuhn KP

    更新日期:1991-06-01 00:00:00

  • Engineering of deglycosylated and plasmin resistant variants of recombinant streptokinase in Pichia pastoris.

    abstract::Streptokinase, a therapeutically important thrombolytic agent, is prone to C-terminal degradation and plasmin-mediated proteolytic processing. Since the protein was glycosylated during secretion from Pichia pastoris, therefore, the role of carbohydrate moieties on its stability was analyzed via in vivo blocking of N-g...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-018-9402-x

    authors: Adivitiya,Babbal,Mohanty S,Khasa YP

    更新日期:2018-12-01 00:00:00

  • Biotechnological production of eleutherosides: current state and perspectives.

    abstract::Eleutherosides, the phenylpropanoid and lignan glycosides, are the active ingredients accumulated in the roots and stems of Eleutherococcus species and in Eleutherococcus senticosus in particular. Syringin (=eleutheroside B) and (-) syringaresinol-di-O-β-D-glucoside (=eleutheroside E) appear as the most important bioa...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-014-5899-9

    authors: Murthy HN,Kim YS,Georgiev MI,Paek KY

    更新日期:2014-09-01 00:00:00

  • A model for penicillin production with and without temperature shift after the growth phase.

    abstract::A strain of Penicillium chrysogenum producing about 8 milligrams/l of penicillin V, was cultivated in a 10-1 bioreactor. Under carbon (C)-limitation during the production phase a glucose/ammonium sulphate mixture was fed using microprocessor control. When the temperature was shifted from 25 degrees C to 30 degrees C a...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00170180

    authors: Kluge M,Siegmund D,Diekmann H,Thoma M

    更新日期:1992-01-01 00:00:00

  • Repeated application of carvone-induced bacteria to enhance biodegradation of polychlorinated biphenyls in soil.

    abstract::Carvone, the principal component of spearmint oil, induces biodegradation of polychlorinated biphenyls (PCB) by Arthrobacter sp. strain B1B. This study investigated the effectiveness of the repeated application of carvone-induced bacteria for bioremediation of Aroclor-1242-contaminated soil. Control treatments compare...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s002530051325

    authors: Gilbert ES,Crowley DE

    更新日期:1998-10-01 00:00:00

  • Role of the Group 2 Mrp sodium/proton antiporter in rapid response to high alkaline shock in the alkaline- and salt-tolerant Dietzia sp. DQ12-45-1b.

    abstract::The six- and seven-subunit Na+/H+ antiporters (Mrp) are widely distributed in bacteria. They are reported to be integral for pH homeostasis in alkaliphilic bacteria when adapting to high pH environments. In this study, operons encoding for the six-subunit Na+/H+ antiporters were found in the genomes of all studied Die...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-018-8846-3

    authors: Fang H,Qin XY,Zhang KD,Nie Y,Wu XL

    更新日期:2018-04-01 00:00:00

  • Optimization of the fermentation and downstream processes for human enterokinase production in Pichia pastoris.

    abstract::Enterokinase is one of the most frequently used enzymes for the removal of affinity tags from target recombinant proteins. In this study, several fermentation strategies were assayed for the production of human enterokinase in Pichia pastoris under constitutive GAP promoter. Two of them with controlled specific growth...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-016-7960-3

    authors: Melicherová K,Krahulec J,Šafránek M,Lišková V,Hopková D,Széliová D,Turňa J

    更新日期:2017-03-01 00:00:00

  • Molecular cloning and nucleotide sequence of the pyruvate kinase gene of an actinomycete Microbispora thermodiastatica.

    abstract::The gene for the thermostable pyruvate kinase of Microbispora thermodiastatica IFO 14046, a moderate thermophilic actinomycete, was cloned in Escherichia coli. This gene consists of an open reading frame of 1422 nucleotides and encodes a protein of 474 amino acids with molecular mass of 50,805 Da. The open reading fra...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s002530051168

    authors: Arai A,Masuda S,Matsuyama A,Murakami S,Nakajima M

    更新日期:1998-03-01 00:00:00

  • Effects of addition of elicitors on rimocidin biosynthesis in Streptomyces rimosus M527.

    abstract::The polyene macrolide rimocidin, produced by Streptomyces rimosus M527, is highly effective against a broad range of fungal plant pathogens, but at low yields. Elicitation is an effective method of stimulating the yield of bioactive secondary metabolites. In this study, the biomass and filtrate of a culture broth of E...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s00253-020-10565-4

    authors: Song Z,Ma Z,Bechthold A,Yu X

    更新日期:2020-05-01 00:00:00

  • Bioengineering studies and pathway modeling of the heterologous biosynthesis of tetrahydrocannabinolic acid in yeast.

    abstract::Heterologous biosynthesis of tetrahydrocannabinolic acid (THCA) in yeast is a biotechnological process in Natural Product Biotechnology that was recently introduced. Based on heterologous genes from Cannabis sativa and Streptomyces spp. cloned into Saccharomyces cerevisiae, the heterologous biosynthesis was fully embe...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-020-10798-3

    authors: Thomas F,Schmidt C,Kayser O

    更新日期:2020-11-01 00:00:00

  • Benefit of Monascus-fermented products for hypertension prevention: a review.

    abstract::γ-Aminobutyric acid (GABA) has been reported to play a neurotransmitter in the central nervous system thereby exerting an inhibition in nerve impulse, in turn ameliorating depression; in addition, recent study also reveals the anti-hypertensive effect of GABA in vivo. Edible fungi of the Monascus species have been use...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s00253-012-4076-2

    authors: Lee BH,Pan TM

    更新日期:2012-06-01 00:00:00