Abstract：
:A strain of Penicillium chrysogenum producing about 8 milligrams/l of penicillin V, was cultivated in a 10-1 bioreactor. Under carbon (C)-limitation during the production phase a glucose/ammonium sulphate mixture was fed using microprocessor control. When the temperature was shifted from 25 degrees C to 30 degrees C at the end of the active growth phase, the specific penicillin production rate was increased by 30%, while the yield remained constant. Maximal productivity without sporulation was obtained when the net growth rate of the active (respiring and producing) biomass, estimated by measuring the respiration rate under defined conditions, was equal to or higher than 0.004 h-1. A model was developed for penicillin fermentation during C-limitation possessing the following properties: (1) the model is based on ordinary differential equations; (2) the influence of different nutrients is considered; (3) the model recognizes two cell types (active and inactive); (4) the model describes the influence of a temperature shift at the end of the vigorous growth phase.
journal_name
Appl Microbiol Biotechnoljournal_title
Applied microbiology and biotechnologyauthors
Kluge M,Siegmund D,Diekmann H,Thoma Mdoi
10.1007/BF00170180subject
Has Abstractpub_date
1992-01-01 00:00:00pages
446-51issue
4eissn
0175-7598issn
1432-0614journal_volume
36pub_type
杂志文章abstract：:Biorefineries allow the production of value-added chemicals, yet this also causes the formation of considerable amounts of wastewater that require suitable treatment. These biorefinery wastewaters often contain a high salinity, which inhibits methanogenesis. In this research, molasses were used to mimic these waste st...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-016-7503-y
更新日期：2016-06-01 00:00:00
abstract：:Bacterial polyhedral organelles are extremely large macromolecular complexes consisting of metabolic enzymes encased within a multiprotein shell that is somewhat reminiscent of a viral capsid. Recent investigations suggest that polyhedral organelles are widely used by bacteria for optimizing metabolic processes. The d...
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abstract：:Diastereoselective reduction of diketones with Lactobacillus kefir DSM 20587 was examined. The reduction of both oxo-functions proceeded highly diastereoselectively. (2 R,5 R)-Hexanediol 3 was produced starting from (2,5)-hexanedione 1 in quantitative yields with enantiomeric excess >99% and diastereomeric excess >99%...
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更新日期：2002-04-01 00:00:00
abstract：:The TonB system functions in iron transport and has been identified in certain Gram-negative bacteria. Recently, we reported three TonB systems in the Aeromonas hydrophila Chinese epidemic strain NJ-35, but the functions of these systems have not been thoroughly elucidated to date. In this study, we investigated the r...
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doi：10.1007/s00253-019-09757-4
更新日期：2019-05-01 00:00:00
abstract：:Inorganic sulfate is the preferred sulfur source for the growth of most microorganisms but, in its absence, many organosulfur compounds can be degraded microbially to provide sulfur. Desulfurization of dibenzothiophene (DBT) by Rhodococcus sp. and of aromatic sulfonates by Pseudomonas sp. has considerable biotechnolog...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章,评审
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更新日期：2001-11-01 00:00:00
abstract：:Currently, increasing attention cancer treatment has focused on molecularly targeted therapies and more recently on immunotherapies targeting immune checkpoints. However, even such advanced treatment may be ineffective. The reasons for this are sought, inter alia, in the human microbiome. In our intestines, there are ...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章,评审
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abstract：:The inhibitory effects of aqueous, ethanolic and dichloromethane fractions from 16 marine algae from the Atlantic shores of North-East Brittany, France, have been investigated against microorganisms frequently associated with immersed surfaces. The extracts were tested in vitro against isolates of marine fungi, bacter...
journal_title：Applied microbiology and biotechnology
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更新日期：2000-10-01 00:00:00
abstract：:An endo-1,4-β-xylanase gene, xylcg, was cloned from Chaetomium globosum and successfully expressed in Escherichia coli. The complete gene of 675 bp was amplified, cloned into the pET 28(a) vector, and expressed. The optimal conditions for the highest activity of the purified recombinant XylCg were observed at a temper...
journal_title：Applied microbiology and biotechnology
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doi：10.1007/s00253-012-4577-z
更新日期：2013-08-01 00:00:00
abstract：:Epidermal growth factor (EGF) ameliorates stress and prevents incomplete gastrointestinal development in early-weaned piglets in commercial swine farming. This study aimed to further analyze the biological activities of intracellularly expressed EGF (IE-EGF), extracellularly expressed EGF (EE-EGF), and tagged EGF (T-E...
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doi：10.1007/s00253-015-6468-6
更新日期：2015-09-01 00:00:00
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journal_title：Applied microbiology and biotechnology
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doi：10.1007/s00253-014-6316-0
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abstract：:Three cytosolic NADPH-dependent flavin-associated proteins (Gox2107, Gox0502, and Gox2684) from Gluconobacter oxydans 621H were overproduced in Escherichia coli, and the recombinant enzymes were purified and characterized. Apparent native molecular masses of 65.2, 78.2, and 78.4 kDa were observed for Gox2107, Gox0502,...
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doi：10.1007/s00253-008-1600-5
更新日期：2008-10-01 00:00:00
abstract：:The growing demand for sustainable animal production is compelling researchers to explore the potential approaches to reduce emissions of greenhouse gases from livestock that are mainly produced by enteric fermentation. Some potential solutions, for instance, the use of chemical inhibitors to reduce methanogenesis, ar...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s00253-013-5365-0
更新日期：2014-01-01 00:00:00
abstract：:Several ansamycins have been reported to inhibit bacterial biofilm formation and accelerate the eradication of developed biofilms, but little is known about the effect of hygrocin C, an ansamycin, on bacterial biofilm formation. Here, hygrocin C was isolated from the marine-derived Streptomyces sp. SCSGAA 0027 and rep...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-017-8672-z
更新日期：2018-02-01 00:00:00
abstract：:Nitric oxide (NO) is known to be involved in modulating production of styrylpyrone polyphenols in the basidiomycete Inonotus obliquus. However, it remains unknown how NO orchestrates fungal styrylpyrone biosynthesis. Here, we show that a transient NO burst correlated with an enhanced expression of phenylalanine ammoni...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-016-7442-7
更新日期：2016-05-01 00:00:00
abstract：:D-Tagatose is a hexoketose monosaccharide sweetener, which is an isomer of D-galactose and is rarely found in nature. Recently, there has been industrial interest in D-tagatose as a low-calorie sugar-substituting sweetener. This article describes the properties and metabolism of tagatose as well as its commercial impo...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s00253-004-1665-8
更新日期：2004-08-01 00:00:00
abstract：:Bacteria, fungi, viruses, and nematodes are the major causal agents of plant diseases. These phytopathogens are responsible for about 10-40% losses in productivity and quality of food crops and horticultural produce. Although eradication of pathogens is not possible, control of plant diseases has been an area of conti...
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pub_type： 杂志文章,评审
doi：10.1007/s00253-019-10334-y
更新日期：2020-02-01 00:00:00
abstract：:Lab-scale systems modelling the spontaneous cocoa bean fermentation process are useful tools to research the influence of process parameters on the fermentation and the final bean quality. In this study in Honduras, a 1-kg lab-scale fermentation (LS-F) was compared to a 300-kg on-farm fermentation (OF-F) in a multipha...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-018-8835-6
更新日期：2018-04-01 00:00:00
abstract：:Catechin is a plant polyphenol composed of epicatechin (EC), epigallocatechin (EGC), epicatechin gallate (ECG), and epigallocatechin gallate (EGCG) as diastereoisomers. Among the various classes of flavonoids, catechin was found to be the most powerful free radical scavenger, scavenging the reactive oxygen species (RO...
journal_title：Applied microbiology and biotechnology
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abstract：:Phomopsis sp. XP-8 is an endophytic fungus that has the ability to produce pinoresinol diglucoside (PDG) in vitro and thus has potential application for the biosynthesis of PDG independent of plants. When cultivated in mung bean medium, PDG production was significantly improved and pinoresinol monoglucoside (PMG) and ...
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journal_title：Applied microbiology and biotechnology
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更新日期：2016-09-01 00:00:00
abstract：:Clonal genotypes resistant to fungal diseases are an important component of the cocoa production system in southeastern Bahia state (Brazil), so that technologies for faster production of stronger and healthier plantlets are highly desirable. In this study, the effects of inoculated bacterial endophytes isolated from ...
journal_title：Applied microbiology and biotechnology
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更新日期：2013-03-01 00:00:00
abstract：:A characteristic mucosal immune response involves the production of antigen-specific secretory immunoglobulin A (SIgA) antibodies. In order to study transcytosis by mimicking the SIgA secretion and to screen for SIgA secretion-promoting substances, we developed a model system of a transfected Madin-Darby canine kidney...
journal_title：Applied microbiology and biotechnology
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doi：10.1007/s00253-015-6501-9
更新日期：2015-07-01 00:00:00
abstract：:Application of soil amendments has been wildly used to increase soil pH and control bacterial wilt. However, little is known about causal shifts in the rhizosphere microbial community of crops, especially when the field naturally harbors the disease of bacterial wilt to tobacco for many years due to long-term continuo...
journal_title：Applied microbiology and biotechnology
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更新日期：2018-11-01 00:00:00
abstract：:The ability of taxonomically different yeasts to synthesize pyruvic acid (PA) from glucose was studied. The study showed that many yeasts are able to produce PA from glucose under the condition of growth limitation by thiamine. This ability was found in the yeast Blastobotrys adeninivorans for the first time. The prod...
journal_title：Applied microbiology and biotechnology
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doi：10.1007/s00253-016-7618-1
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abstract：:Chitin was purified from Penaeus monodon and Crangon crangon shells using a two-stage fermentation process with anaerobic deproteination followed by decalcification through homofermentative lactic acid fermentation. Deproteinating enrichment cultures from sewage sludge and ground meat (GM) were used with a proteolytic...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
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更新日期：2008-06-01 00:00:00
abstract：:The present study reports the successful production of human pre-miR-29b both intra- and extracellularly in the marine phototrophic bacterium Rhodovulum sulfidophilum using recombinant RNA technology. In a first stage, the optimal transformation conditions (0.025 μg of plasmid DNA, with a heat-shock of 2 min at 35 °C)...
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更新日期：2016-04-01 00:00:00
abstract：:Human neutrophil peptide 1 (HNP1) is a small (3.44 kDa) cationic peptide that is a distinct member of the defensin family. HNP1 plays a crucial role in controlling bacterial infections, particularly by antibiotic-resistant bacteria, through membrane perforation patterns. The structural characteristics of HNP1's three ...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-018-8982-9
更新日期：2018-06-01 00:00:00
abstract：:The exclusive use of isopropyl beta-D-thiogalactopyranoside to activate the T7 promoter for protein production has limited the general use of the expression system. We have sought an alternative by constructing a recombinant Escherichia coli strain, BL21 (G2), to carry a chromosomal copy of T7 gene 1 fused to the lamb...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-001-0891-6
更新日期：2002-03-01 00:00:00
abstract：:A freshwater algicidal bacterial strain, Lzh-5, isolated from Lake Taihu, with strong algicidal activity against Microcystis aeruginosa, was identified as Bacillus sp. based on its phenotypic characteristics and 16S ribosomal RNA (rRNA) gene sequence. The algicidal mode of Bacillus sp. Lzh-5 was indirect, attacking M....
journal_title：Applied microbiology and biotechnology
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doi：10.1007/s00253-014-6043-6
更新日期：2015-01-01 00:00:00
