What renders Bacilli genetically competent? A gaze beyond the model organism.

abstract：:A drawback of biotechnological processes, where microorganisms convert biomass constituents, such as starch, cellulose, hemicelluloses, lipids, and proteins, into wanted products, is the economic feasibility. Particularly the cost of nitrogen sources in biotechnological processes can make up a large fraction of total ...

journal_title：Applied microbiology and biotechnology

authors： Pleissner D,Venus J

更新日期：2016-03-01 00:00:00

abstract：:Time-resolved analysis assays of receptor-ligand interactions are fundamental in basic research and drug discovery. Adequate methods are well developed for the analysis of recombinant proteins such as antibody-antigen interactions. However, assays for time-resolved ligand-binding processes on living cells are still ra...

journal_title：Applied microbiology and biotechnology

authors： Encarnação JC,Schulte T,Achour A,Björkelund H,Andersson K

更新日期：2018-05-01 00:00:00

abstract：:Enzymatic kinetic resolution is a widely used biotechnological tool for the production of enantiomerically pure/enriched compounds. This technique takes advantage of the enantioselectivity or enantiospecificity of an enzyme for one of the enantiomers of a racemic substrate to isolate the desired isomer. N-Carbamoyl-D-...

journal_title：Applied microbiology and biotechnology

authors： Martínez-Rodríguez S,Martínez-Gómez AI,Rodríguez-Vico F,Clemente-Jiménez JM,Las Heras-Vázquez FJ

更新日期：2010-01-01 00:00:00

abstract：:Biotechnologies that aim to produce renewable fuels, chemicals, and bioproducts from residual ligno(hemi)cellulosic biomass mostly rely on enzymatic depolymerization of plant cell walls (PCW). This process requires an arsenal of diverse enzymes, including xylanases, which synergistically act on the hemicellulose, redu...

journal_title：Applied microbiology and biotechnology

authors： Evangelista DE,de Oliveira Arnoldi Pellegrini V,Santo ME,McQueen-Mason S,Bruce NC,Polikarpov I

更新日期：2019-10-01 00:00:00

abstract：:Chemical mutagenesis of the phototrophic green microalga Parietochloris incisa, producing high amounts of arachidonic acid (ARA), resulted in selection of a mutant deficient in ARA and rich in dihomo-γ-linolenic acid (DGLA) and thus ∆5 desaturase defective. The mutagenesis produced a nonsense mutation in the ∆5 desatu...

journal_title：Applied microbiology and biotechnology

authors： Iskandarov U,Khozin-Goldberg I,Cohen Z

更新日期：2011-04-01 00:00:00

abstract：:Mutagenesis and selection were applied to a strain of Penicillium echinulatum by treating conidia with hydrogen peroxide or 1,2,7,8-diepoxyoctane and then by incubating the conidia for 48 h in broth containing microcrystalline cellulose washed in 0.5% (w/v) aqueous 2-deoxyglucose before plating them onto cellulose aga...

journal_title：Applied microbiology and biotechnology

authors： Dillon AJ,Zorgi C,Camassola M,Henriques JA

更新日期：2006-05-01 00:00:00

abstract：:Transfer of a biosynthetic pathway between evolutionary distant organisms can create a metabolic shunt capable of bypassing the native regulation of the host organism, hereby improving the production of secondary metabolite precursor molecules for important natural products. Here, we report the engineering of Escheric...

journal_title：Applied microbiology and biotechnology

authors： Carlsen S,Ajikumar PK,Formenti LR,Zhou K,Phon TH,Nielsen ML,Lantz AE,Kielland-Brandt MC,Stephanopoulos G

更新日期：2013-07-01 00:00:00

abstract：:A thermophilic Bacillus coagulans WCP10-4 with tolerance to high concentration of glucose was isolated from soil and used to produce optically pure L-lactic acid from glucose and starch. In batch fermentation at pH 6.0, 240 g/L of glucose was completely consumed giving 210 g/L of L-lactic acid with a yield of 95 % and...

journal_title：Applied microbiology and biotechnology

authors： Zhou X,Ye L,Wu JC

更新日期：2013-05-01 00:00:00

abstract：:Photosynthetic microorganisms produce relatively large amounts of physiologically active materials which stimulate the physiological activity of other organisms. In this study, mammalian HeLa cells were cultured in different culture media which were Dulbecco's modified Eagle medium (DMEM) with newborn calf serum (NCS)...

journal_title：Applied microbiology and biotechnology

authors： Lee HJ,Park JY,Yoo KS,Yoon J,Kim YH,Min J

更新日期：2013-11-01 00:00:00

abstract：:Orange peel waste (OPW), the primary byproduct of the juice extraction process, is annually generated in massive amounts (21 Mton), and its aqueous extraction in biorefining operations yields a liquid fraction, referred to as orange peel extract (OPE). Although OPE contains significant amounts of easily assimilable ca...

journal_title：Applied microbiology and biotechnology

authors： Carota E,Petruccioli M,D'Annibale A,Gallo AM,Crognale S

更新日期：2020-05-01 00:00:00

abstract：:Dengue virus (DENV) infection is one of the most important public health threats globally; however, no vaccines or effective antivirals are currently available. The bioluminescence imaging technique has emerged as a powerful tool for studies on viral pathogenesis in vitro and in vivo. In this study, using a recombinan...

journal_title：Applied microbiology and biotechnology

authors： Li XF,Deng YQ,Zhao H,Ye Q,Wang HJ,Li SH,Zhu SY,Shi PY,Qin ED,Zhang B,Qin CF

更新日期：2013-05-01 00:00:00

abstract：:This review presents developments and applications in bioleaching and mineral biooxidation since publication of a previous mini review in 2003 (Olson et al. Appl Microbiol Biotechnol 63:249-257, 2003). There have been discoveries of newly identified acidophilic microorganisms that have unique characteristics for effec...

journal_title：Applied microbiology and biotechnology

authors： Brierley CL,Brierley JA

更新日期：2013-09-01 00:00:00

abstract：:Fatty acid-binding protein 2 (Fabp2), which is involved in the transport of long-chain fatty acids, is widely studied in mammals. Nevertheless, the role of this protein in teleost fish is mostly unknown. Here, we produced a fabp2-/- zebrafish (KO) animal model. Compared with wild-type zebrafish (WT), KO had a markedly...

journal_title：Applied microbiology and biotechnology

authors： Zhao Y,Cao X,Fu L,Gao J

更新日期：2020-03-01 00:00:00

abstract：:We have fused the epidermal growth factor (EGF) to the amino terminus of Pseudomonas exotoxin A (PE) to create a cytotoxic agent, designated EGF-PE, which preferentially kills EGF-receptor-bearing cells. In this study, we analyzed the effect of the Ia domain, the binding domain of PE on the cytotoxicity of EGF-PE towa...

journal_title：Applied microbiology and biotechnology

authors： Liao CW,Hseu TH,Hwang J

更新日期：1995-07-01 00:00:00

abstract：:Repetitious gene cassettes that encode the consensus decapeptide repeat of Mytilus edulis bioadhesive protein were designed, constructed, and expressed in Escherichia coli. The bioadhesive precursor (BP) with a relative molecular mass of 25,000 was expressed from one 600-bp gene at levels approaching 60% of total cell...

journal_title：Applied microbiology and biotechnology

authors： Salerno AJ,Goldberg I

更新日期：1993-05-01 00:00:00

abstract：:A precise and scarless genome excision method, employing the Cre/loxP system in concert with double-strand break (DSB)-stimulated intramolecular recombination was developed. The DSBs were mediated by the restriction endonuclease, I-SceI. It permitted multiple deletions of independent 14-, 43-, and 10-kb-long genomic r...

journal_title：Applied microbiology and biotechnology

authors： Suzuki N,Nonaka H,Tsuge Y,Okayama S,Inui M,Yukawa H

更新日期：2005-11-01 00:00:00

abstract：:γ-Aminobutyric acid (GABA) has been reported to play a neurotransmitter in the central nervous system thereby exerting an inhibition in nerve impulse, in turn ameliorating depression; in addition, recent study also reveals the anti-hypertensive effect of GABA in vivo. Edible fungi of the Monascus species have been use...

journal_title：Applied microbiology and biotechnology

authors： Lee BH,Pan TM

更新日期：2012-06-01 00:00:00

abstract：:Neuritin (also known as candidate plasticity gene 15 (cpg15)) is a neurotrophic factor that was recently discovered in a screen aimed at identifying genes involved in activity-dependent synaptic plasticity. Neuritin plays multiple roles in both neural development (Chen et al. Zhonghua Yan Ke Za Zhi 46:978-983 2010; Co...

journal_title：Applied microbiology and biotechnology

authors： Zhang Y,Zhang S,Xian L,Tang J,Zhu J,Cui L,Li S,Yang L,Huang J

更新日期：2015-10-01 00:00:00

abstract：:Conventional acetone-butanol-ethanol (ABE) fermentation coupled with gas stripping is conducted under strict anaerobic conditions. In this work, a fed-batch ABE fermentation integrated with gas stripping (FAFIGS) system using a non-strict anaerobic butanol-producing symbiotic system, TSH06, was investigated for the ef...

journal_title：Applied microbiology and biotechnology

authors： Lin Z,Liu H,Yan X,Zhou Y,Cheng K,Zhang J

更新日期：2017-11-01 00:00:00

abstract：:Transcription factors have been studied as an important drug candidate. Ever since the successful generation of induced pluripotent stem cells (iPSCs), there has been tremendous interest in reprogramming transcription factors. Because of the safety risks involved in a virus-based approach, many researchers have been t...

journal_title：Applied microbiology and biotechnology

authors： Ryu J,Park HH,Park JH,Lee HJ,Rhee WJ,Park TH

更新日期：2016-04-01 00:00:00

abstract：:Crude peroxidase preparations from the lignocellulose-degrading actinomycete, Streptomyces viridosporus T7A, were shown to decolorize several azo dye isomers and showed a correlation of dye structure to degradability similar to that shown by fungal Mn-peroxidase, an enzyme not previously described in actinomycetes. Ad...

journal_title：Applied microbiology and biotechnology

authors： Burke NS,Crawford DL

更新日期：1998-05-01 00:00:00

abstract：:Hydroxy fatty acids (HFA) have gained importance because of their special properties such as higher viscosity and reactivity compared with other non-hydroxy fatty acids. The bacterial isolate Pseudomonas aeruginosa (PR3) was reported to produce mono-, di-, and trihydroxy fatty acids from different unsaturated fatty ac...

journal_title：Applied microbiology and biotechnology

authors： Chang IA,Kim IH,Kang SC,Hou CT,Kim HR

更新日期：2007-02-01 00:00:00

abstract：:The production of infective juvenile stages (IJ) of the entomopathogenic nematode Steinernema carpocapsae in the presence of its symbiotic bacterium Xenorhabdus nematophilus was carried out in orbitally agitated bottles. Four complex culture media (M1-M4) were used, containing from 8% to 28% (by vol.) agave juice (agu...

journal_title：Applied microbiology and biotechnology

authors： Islas-López MA,Sanjuan-Galindo R,Rodríguez-Hernández AI,Chavarría-Hernández N

更新日期：2005-07-01 00:00:00

abstract：:The Escherichia coli RNase G is known as an endoribonuclease responsible for the 5'-end maturation of 16S rRNA and degradation of several specific mRNAs such as adhE and eno mRNAs. In this study, we found that an RNase G mutant derived from the MC1061 strain did not grow on a glucose minimal medium. Genetic analysis r...

journal_title：Applied microbiology and biotechnology

authors： Sakai T,Nakamura N,Umitsuki G,Nagai K,Wachi M

更新日期：2007-08-01 00:00:00

abstract：:The osmotic tolerance of cells of Saccharomyces cerevisiae as a function of glycerol concentration and temperature has been investigated. Results show that under isothermal conditions (25 degrees C) cells are resistant (94% viability) to hyperosmotic treatment at 49.2 MPa. A thigher osmotic pressure, cell viability de...

journal_title：Applied microbiology and biotechnology

authors： Beney L,Marechal PA,Gervais P

更新日期：2001-08-01 00:00:00

abstract：:The physiological effects of 5-hydroxymethylfurfural (HMF) on Saccharomyces cerevisiae CBS 8066 in the presence and absence of furfural were studied. Experiments were carried out by pulse addition of HMF (2-4 g/l) as well as HMF (2 g/l) together with furfural (2 g/l) to batch cultivations of S. cerevisiae. Synthetic m...

journal_title：Applied microbiology and biotechnology

authors： Taherzadeh MJ,Gustafsson L,Niklasson C,Lidén G

更新日期：2000-06-01 00:00:00

abstract：:Eight Escherichia coli strains were studied in minimal medium with a continuous flow system using confocal microscopy. K12 wild-type strains ATCC 25404 and MG1655 formed the best biofilms ( approximately 43 microm thick, 21 to 34% surface coverage). JM109, DH5alpha, and MG1655 motA formed intermediate biofilms ( appro...

journal_title：Applied microbiology and biotechnology

authors： Wood TK,González Barrios AF,Herzberg M,Lee J

更新日期：2006-09-01 00:00:00

abstract：:The dawn of a new Proteomics era, just over a decade ago, allowed for large-scale protein profiling studies that have been applied in the identification of distinctive molecular cell signatures. Proteomics provides a powerful approach for identifying and studying these multiple molecular markers in a vast array of bio...

journal_title：Applied microbiology and biotechnology

authors： da Costa JP,Carvalhais V,Ferreira R,Amado F,Vilanova M,Cerca N,Vitorino R

更新日期：2015-09-01 00:00:00

abstract：:To reveal the succession procedure of ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) community structure in sequencing biofilm batch reactor (SBBR), the molecular biological techniques of denaturing gradient gel electrophoresis (DGGE), cloning, and real-time PCR were applied. DGGE showed that th...

journal_title：Applied microbiology and biotechnology

authors： Wang J,Zhang C,Rong H

更新日期：2014-05-01 00:00:00

abstract：:Cultures of Umbelopsis ramanniana (=Mucor ramannianus) were grown in fluid Sabouraud medium for 3 days, dosed with 0.23 mM benzo[f]quinoline, benzo[h]quinoline, or phenanthridine (benzo[c]quinoline), and incubated for another 18 days. Cultures were extracted and metabolites (66-75% of the UV absorbance) were separated...

journal_title：Applied microbiology and biotechnology

authors： Sutherland JB,Cross EL,Heinze TM,Freeman JP,Moody JD

更新日期：2005-05-01 00:00:00