Noninvasive bioluminescence imaging of dengue virus infection in the brain of A129 mice.


:Dengue virus (DENV) infection is one of the most important public health threats globally; however, no vaccines or effective antivirals are currently available. The bioluminescence imaging technique has emerged as a powerful tool for studies on viral pathogenesis in vitro and in vivo. In this study, using a recombinant DENV that stably expressed Renilla luciferase (Rluc-DENV), we used bioluminescence for imaging of DENV infection in the brain of A129 mice that lacked type I interferon receptors. Upon intracranial inoculation with Rluc-DENV, A129 mice developed typical neurological symptoms and rapidly succumbed to viral infection. Real-time bioluminescence intensity analysis revealed the replication kinetics of Rluc-DENV in the brain of A129 mice. Linear regression analyses showed a good correlation between photon flux and viral titers (R(2) = 0.9923). Finally, the bioluminescence model was validated using a known mouse monoclonal antibody, 2A10G6, and the therapeutic effects of this neutralizing antibody were readily monitored by live imaging in the same animal. The noninvasive bioluminescence imaging of DENV infection as described here shows distinct advantages over traditional animal models and provides a powerful tool for potential antiviral or vaccine assays against DENV infection in vivo.


Li XF,Deng YQ,Zhao H,Ye Q,Wang HJ,Li SH,Zhu SY,Shi PY,Qin ED,Zhang B,Qin CF




Has Abstract


2013-05-01 00:00:00












  • Microbial aspartic proteases: current and potential applications in industry.

    abstract::Aspartic proteases are a relatively small group of proteolytic enzymes that are active in acidic environments and are found across all forms of life. Certain microorganisms secrete such proteases as virulence agents and/or in order to break down proteins thereby liberating assimilable sources of nitrogen. Some of the ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审


    authors: Theron LW,Divol B

    更新日期:2014-11-01 00:00:00

  • Quantitative monitoring of 2-oxoglutarate in Escherichia coli cells by a fluorescence resonance energy transfer-based biosensor.

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    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Zhang C,Wei ZH,Ye BC

    更新日期:2013-09-01 00:00:00

  • A control strategy to investigate the relationship between specific productivity and high-mannose glycoforms in CHO cells.

    abstract::The integration of physiological knowledge into process control strategies is a cornerstone for the improvement of biopharmaceutical cell culture technologies. The present contribution investigates the applicability of specific productivity as a physiological control parameter in a cell culture process producing a mon...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Zalai D,Hevér H,Lovász K,Molnár D,Wechselberger P,Hofer A,Párta L,Putics Á,Herwig C

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  • Release of thioredoxin from Saccharomyces cerevisiae with environmental stimuli: solubilization of thioredoxin with ethanol.

    abstract::Thioredoxin is crucial for the maintenance of the redox status of cells of all types. Mammalian thioredoxin is secreted from various types of cells, although the mechanism underlying has not yet been clarified. Previously, we demonstrated that thioredoxin was released from Saccharomyces cerevisiae after treatment with...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Takeuchi Y,Nomura W,Ohdate T,Tamasu S,Masutani H,Murata K,Izawa S,Yodoi J,Inoue Y

    更新日期:2007-07-01 00:00:00

  • Rapid adaptation of Rhodococcus erythropolis cells to salt stress by synthesizing polyunsaturated fatty acids.

    abstract::Bacterial cells are known to adapt to challenging environmental conditions such as osmotic stress. However, most of the work done in this field describes the adaptation of growing populations where the new generations acquire traits that improve their ability to survive. In the present study, the responses of Rhodococ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: de Carvalho CC,Marques MP,Hachicho N,Heipieper HJ

    更新日期:2014-06-01 00:00:00

  • An efficient lipase-catalyzed enantioselective hydrolysis of (R,S)-azolides derived from N-protected proline, pipecolic acid, and nipecotic acid.

    abstract::In the Candida antarctica lipase B-catalyzed hydrolysis of (R,S)-azolides derived from (R,S)-N-protected proline in water-saturated methyl tert-butyl ether (MTBE), high enzyme activity with excellent enantioselectivity (V (S) V (R) ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Wu CH,Pen CW,Wang PY,Tsai SW

    更新日期:2013-02-01 00:00:00

  • High-yield growth and magnetosome formation by Magnetospirillum gryphiswaldense MSR-1 in an oxygen-controlled fermentor supplied solely with air.

    abstract::Magnetotactic bacteria are difficult to grow under defined conditions in culture, which has presented a major obstacle to commercial application of magnetosomes. We studied the relationships among the cell growth, magnetosome formation, dissolved oxygen concentration (DO), and the ability to supply oxygen to the cells...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


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    更新日期:2008-06-01 00:00:00

  • Parameters affecting the degradation of benzothiazoles and benzimidazoles in activated sludge systems.

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    pub_type: 杂志文章


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    更新日期:1993-07-01 00:00:00

  • Cholesterol oxidase ChoL is a critical enzyme that catalyzes the conversion of diosgenin to 4-ene-3-keto steroids in Streptomyces virginiae IBL-14.

    abstract::Diosgenin transformation was studied in Streptomyces virginiae IBL-14, a soil-dwelling bacterium with diosgenin-degrading capacity. All of the derivatives isolated were identified as 4-ene-3-keto steroids. We cloned ChoL, a fragment of a cholesterol oxidase from S. virginiae IBL-14, and used gene-disruption techniques...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Li B,Wang W,Wang FQ,Wei DZ

    更新日期:2010-02-01 00:00:00

  • Alpha- and beta-tubulin from Phytophthora capsici KACC 40483: molecular cloning, biochemical characterization, and antimicrotubule screening.

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    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


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    更新日期:2009-03-01 00:00:00

  • Ammonia manipulates the ammonia-oxidizing archaea and bacteria in the coastal sediment-water microcosms.

    abstract::Ammonia was observed as a potential significant factor to manipulate the abundance and activity of ammonia-oxidizing microorganisms (AOMs) in water environments. For the first time, this study confirmed this phenomenon by laboratory cultivation. In a series of estuarine sediment-coastal water microcosms, we investigat...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Zhang Y,Chen L,Dai T,Sun R,Wen D

    更新日期:2015-08-01 00:00:00

  • Role of viral suppressors governing asymmetric synergism between tomato-infecting begomoviruses.

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    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Basu S,Singh AK,Singh D,Sahu SK,Chakraborty S

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  • Optimization of the extracellular production of a bacterial phytase with Escherichia coli by using different fed-batch fermentation strategies.

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    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


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  • Genetic polymorphism of ferula mushroom growing on Ferula sinkiangensis.

    abstract::Mating tests, internal transcribed spacer (ITS) sequence analysis, intergenic spacer 1-restriction fragment length polymorphism (IGS1-RFLP), IGS1 sequence analysis, and IGS2-RFLP analysis were carried out on isolates of 17 morphologically different Pleurotus mushrooms collected on Ferula sinkiangensis. The isolates we...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Zhang JX,Huang CY,Ng TB,Wang HX

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  • Enhanced enantioselectivity of a carboxyl esterase from Rhodobacter sphaeroides by directed evolution.

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    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Ma J,Wu L,Guo F,Gu J,Tang X,Jiang L,Liu J,Zhou J,Yu H

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  • Metabolic engineering of Corynebacterium glutamicum for L-cysteine production.

    abstract::L-cysteine, a valuable sulfur-containing amino acid, has been widely used in food, agriculture, and pharmaceutical industries. Due to the toxicity and complex regulation of L-cysteine, no efficient cell factory has yet been achieved for L-cysteine industrial production. In this study, the food-grade microorganism Cory...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Wei L,Wang H,Xu N,Zhou W,Ju J,Liu J,Ma Y

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  • Generalizing a hybrid synthetic promoter approach in Yarrowia lipolytica.

    abstract::Both varied and strong promoters are essential for metabolic and pathway engineering applications in any host organism. To enable this capacity, here we demonstrate a generalizable method for the de novo construction of strong, synthetic hybrid promoter libraries. Specifically, we demonstrate how promoter truncation a...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


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    更新日期:2013-04-01 00:00:00

  • Growth of Escherichia coli MG1655 on LB medium: monitoring utilization of amino acids, peptides, and nucleotides with transcriptional microarrays.

    abstract::Analysis of gene expression data related to assimilation and biosynthesis of nitrogen-containing compounds amino acids, peptides, and nucleotides was used to monitor availability of these nutrients to Escherichia coli MG1655 growing on Luria-Bertani medium. The data indicate that free amino acids and nucleotides only ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Baev MV,Baev D,Radek AJ,Campbell JW

    更新日期:2006-07-01 00:00:00

  • Development of chitosan-coated agar-gelatin particles for probiotic delivery and targeted release in the gastrointestinal tract.

    abstract::This study reports the development of a novel and simple formulation for probiotic delivery using chitosan-coated agar-gelatin gel particles. This methodology involves the production of agar-gelatin particles by thermally treating a mixture of agar and gelatin solutions at high temperatures (121 °C) and subsequently c...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


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    更新日期:2020-07-01 00:00:00

  • AveI, an AtrA homolog of Streptomyces avermitilis, controls avermectin and oligomycin production, melanogenesis, and morphological differentiation.

    abstract::Streptomyces avermitilis is well known as the producer of anthelmintic agent avermectins, which are widely used in agriculture, veterinary medicine, and human medicine. aveI encodes a TetR-family regulator, which is the homolog of AtrA. It was reported that deletion of aveI caused enhanced avermectin production. In th...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Liu L,Cheng Y,Lyu M,Zhao X,Wen Y,Li J,Chen Z

    更新日期:2019-10-01 00:00:00

  • Inhibition of the development of microorganisms (bacteria and fungi) by extracts of marine algae from Brittany, France.

    abstract::The inhibitory effects of aqueous, ethanolic and dichloromethane fractions from 16 marine algae from the Atlantic shores of North-East Brittany, France, have been investigated against microorganisms frequently associated with immersed surfaces. The extracts were tested in vitro against isolates of marine fungi, bacter...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


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    更新日期:2000-10-01 00:00:00

  • Amplification of three threonine biosynthesis genes in Corynebacterium glutamicum and its influence on carbon flux in different strains.

    abstract::The hom-thrB operon (homoserine dehydrogenase/homoserine kinase) and the thrC gene (threonine synthase) of Corynebacterium glutamicum ATCC 13,032 and the homFBR (homoserine dehydrogenase resistant to feedback inhibition by threonine) alone as well as homFBR-thrB operon of C. glutamicum DM 368-3 were cloned separately ...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Eikmanns BJ,Metzger M,Reinscheid D,Kircher M,Sahm H

    更新日期:1991-02-01 00:00:00

  • Substrate selectivity of various lipases in the esterification of cis- and trans-9-octadecenoic acid.

    abstract::The substrate selectivity of numerous commercially available lipases from microorganisms, plants and animal tissue towards 9-octadecenoic acids with respect to the cis/trans configuration of the C=C double bond was examined by the esterification of cis- and trans-9-octadecanoic acid (oleic and elaidic acid respectivel...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Borgdorf R,Warwel S

    更新日期:1999-04-01 00:00:00

  • Azo-dye degradation in an anaerobic-aerobic treatment system operating on simulated textile effluent.

    abstract::Decolorisation of azo dyes during biological effluent treatment can involve both adsorption to cell biomass and degradation by azo-bond reduction during anaerobic digestion. Degradation is expected to form aromatic amines, which may be toxic and recalcitrant to anaerobic treatment but degradable aerobically. Methods f...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: O'Neill C,Lopez A,Esteves S,Hawkes FR,Hawkes DL,Wilcox S

    更新日期:2000-02-01 00:00:00

  • Fine mapping of linear B cell epitopes on capsid protein of porcine circovirus 3.

    abstract::Porcine circovirus type 3 (PCV3) is an emerging swine pathogen associated with acute porcine dermatitis and nephropathy syndrome (PDNS)-like clinical signs, reproductive failure, and multisystemic inflammation. Current evidence shows that PCV3 is spread worldwide, and its high incidence may pose a threat to the global...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Jiang M,Guo J,Zhang G,Jin Q,Liu Y,Jia R,Wang A

    更新日期:2020-07-01 00:00:00

  • Survey of microbes in industrial-scale second-generation bioethanol production for better process knowledge and operation.

    abstract::The microbes present in bioethanol production processes have been previously studied in laboratory-scale experiments, but there is a lack of information on full-scale industrial processes. In this study, the microbial communities of three industrial bioethanol production processes were characterized using several meth...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Aho VTE,Tolonen T,Haverinen J,Jaakkola M,Paulin L,Auvinen P,Laine MM

    更新日期:2020-09-01 00:00:00

  • Desulfurization and desulfonation: applications of sulfur-controlled gene expression in bacteria.

    abstract::Inorganic sulfate is the preferred sulfur source for the growth of most microorganisms but, in its absence, many organosulfur compounds can be degraded microbially to provide sulfur. Desulfurization of dibenzothiophene (DBT) by Rhodococcus sp. and of aromatic sulfonates by Pseudomonas sp. has considerable biotechnolog...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章,评审


    authors: Kertesz MA,Wietek C

    更新日期:2001-11-01 00:00:00

  • Characterization of the superoxide dismutase SOD1 gene of Kluyveromyces marxianus L3 and improved production of SOD activity.

    abstract::Superoxide dismutase (SOD) activity is one major defense line against oxidative stress for all of the aerobic organisms, and industrial production of this enzyme is highly demanded. The Cu/Zn superoxide dismutase gene (KmSOD1) of Kluyveromyces marxianus L3 was cloned and characterized. The deduced KmSod1p protein shar...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Raimondi S,Uccelletti D,Matteuzzi D,Pagnoni UM,Rossi M,Palleschi C

    更新日期:2008-01-01 00:00:00

  • Lysine biosynthesis in microbes: relevance as drug target and prospects for β-lactam antibiotics production.

    abstract::Plants as well as pro- and eukaryotic microorganisms are able to synthesise lysine via de novo synthesis. While plants and bacteria, with some exceptions, rely on variations of the meso-diaminopimelate pathway for lysine biosynthesis, fungi exclusively use the α-aminoadipate pathway. Although bacteria and fungi are, i...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Fazius F,Zaehle C,Brock M

    更新日期:2013-05-01 00:00:00

  • Characterization of a methane-oxidizing biofilm using microarray, and confocal microscopy with image and geostatic analyses.

    abstract::A mixed methane-oxidizing biofilm was characterized, concurrently using a number of advanced techniques. Community analysis results by microarray exhibited that type II members dominated the methanotrophic community, in which Methylocystis was most abundant, followed by Methylosinus. Observation results by fluorescent...

    journal_title:Applied microbiology and biotechnology

    pub_type: 杂志文章


    authors: Kim TG,Yi T,Lee EH,Ryu HW,Cho KS

    更新日期:2012-08-01 00:00:00