当前位置： SCI文献检索 > APPLIED MICROBIOLOGY AND BIOTECHNOLOGY期刊下所有文献 > A mutant trypsin-like enzyme from Streptomyces fradiae, created by site-directed mutagenesis, improves affinity chromatography for protein trypsin inhibitors.

A mutant trypsin-like enzyme from Streptomyces fradiae, created by site-directed mutagenesis, improves affinity chromatography for protein trypsin inhibitors.

Abstract：

:The Ser-170 residue of a trypsin-like enzyme from Streptomyces fradiae (SFT), which is considered to be the active-site serine, was replaced with alanine by site-directed mutagenesis to improve the affinity chromatography step for a Kazal-type trypsin inhibitor pancreatic secretory trypsin inhibitor (PSTI). The resulting mutant SFT, designated as [S170A]SFT, was expressed in Streptomyces lividans and purified to homogeneity. [S170A]SFT was catalytically inactive, but still had the ability to bind tightly to PSTI and to soybean trypsin inhibitor with dissociation constants of 3.1 x 10(-7) M and 1.9 x 10(-8) M respectively. We further demonstrated that recombinant human PSTI secreted into Saccharomyces cerevisiae culture broth could be purified to homogeneity with a one-step [S170A]SFT-affinity column. The purified PSTI contained no molecules intramolecularly cleaved by active trypsin, which are found when trypsin-affinity chromatography is used for the purification. This eliminated the need for further separation of intact PSTI from intramolecularly cleaved PSTI by high-performance liquid chromatography, thus simplifying and improving its purification process.

journal_name

Appl Microbiol Biotechnol

journal_title

Applied microbiology and biotechnology

authors

Katoh T,Kikuchi N,Nagata K,Yoshida N

doi

10.1007/s002530050777

subject

Has Abstract

pub_date

1996-08-01 00:00:00

pages

15-21

issue

1

eissn

0175-7598

issn

1432-0614

journal_volume

46

pub_type

杂志文章

相关文献

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY文献大全
  • Regulation of trichothecene biosynthesis in Fusarium: recent advances and new insights.

    abstract：:Trichothecenes are toxic secondary metabolites produced by filamentous fungi mainly belonging to the Fusarium genus. Production of these mycotoxins occurs during infection of crops and is a threat to human and animal health. Although the pathway for biosynthesis of trichothecenes is well established, the regulation of...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章,评审

    doi：10.1007/s00253-011-3397-x

    authors： Merhej J,Richard-Forget F,Barreau C

    更新日期：2011-08-01 00:00:00

  • Abundance and diversity of iron reducing bacteria communities in the sediments of a heavily polluted freshwater lake.

    abstract：:Iron reduction mediated by Fe(III)-reducing bacteria (FeRB) occurs in aqueous environments and plays an essential role in removing contaminates in polluted freshwater lakes. Two model FeRB species, Shewanella and Geobacter, have been intensively studied because of their functions in bioremediation, iron reduction, and...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-018-9443-1

    authors： Fan YY,Li BB,Yang ZC,Cheng YY,Liu DF,Yu HQ

    更新日期：2018-12-01 00:00:00

  • Development of Tomato bushy stunt virus-based vectors for fusion and non-fusion expression of heterologous proteins in an alternative host Nicotiana excelsiana.

    abstract：:Plant virus-based expression systems are an alternative expression platform for the production of clinically and industrially useful recombinant proteins. Nonetheless, due to a lack of viral vector with the commercial potentials, it is urgent to design and develop new, versatile, and efficient plant virus vectors. The...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-020-10837-z

    authors： Zhang X,Ding X,Li Z,Wang S

    更新日期：2020-10-01 00:00:00

  • Distribution of Oenococcus oeni populations in natural habitats.

    abstract：:Oenococcus oeni is the lactic acid bacteria species most commonly encountered in wine, where it develops after the alcoholic fermentation and achieves the malolactic fermentation that is needed to improve the quality of most wines. O. oeni is abundant in the oenological environment as well as in apple cider and kombuc...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章,评审

    doi：10.1007/s00253-019-09689-z

    authors： Lorentzen MPG,Lucas PM

    更新日期：2019-04-01 00:00:00

  • Functions and impact of tal-like genes in animals with regard to applied aspects.

    abstract：:A large number of DNAs in eukaryote genomes can code for atypical transcripts, and their functions are controversial. It has been reported that the transcripts contain many small open reading frames (sORFs), which were originally considered as non-translatable RNAs. However, increasing evidence has suggested that some...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章,评审

    doi：10.1007/s00253-018-9159-2

    authors： Zhu M,Hu X,Cao G,Xue R,Gong C

    更新日期：2018-08-01 00:00:00

  • Purification and characterization of thermostable H2O2-forming NADH oxidase from 2-phenylethanol-assimilating Brevibacterium sp. KU1309.

    abstract：:A cytoplasmic NADH oxidase (NOX) was purified from a soil bacteria, Brevibacterium sp. KU1309, which is able to grow in the medium containing 2-phenylethanol as the sole source of carbon under an aerobic condition. The enzyme catalyzed the oxidation of NADH to NAD+ involving two-electron reduction of O2 to H2O2. The m...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-008-1535-x

    authors： Hirano J,Miyamoto K,Ohta H

    更新日期：2008-08-01 00:00:00

  • Microencapsulation of new probiotic formulations for gastrointestinal delivery: in vitro study to assess viability and biological properties.

    abstract：:The paper describes the preparation of new probiotic formulations based on chitosan-coated alginate microcapsules containing three different probiotic strains, Lactobacillus plantarum PBS067, Lactobacillus rhamnosus PBS070, and Bifidobacterium animalis subsp. lactis PBS075 taken individually and as a mixture of them. ...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-015-6853-1

    authors： D'Orazio G,Di Gennaro P,Boccarusso M,Presti I,Bizzaro G,Giardina S,Michelotti A,Labra M,La Ferla B

    更新日期：2015-11-01 00:00:00

  • Gongronella sp. w5 elevates Coprinopsis cinerea laccase production by carbon source syntrophism and secondary metabolite induction.

    abstract：:When sucrose was used as the carbon source, the Basidiomycete Coprinopsis cinerea showed poor growth and low laccase activity in pure culture, but greatly enhanced the level of laccase activity (>1800 U/L) during coculture with the Mucoromycete Gongronella sp. w5. As a result, the mechanism of laccase overproduction i...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-018-9469-4

    authors： Hu J,Zhang Y,Xu Y,Sun Q,Liu J,Fang W,Xiao Y,Kües U,Fang Z

    更新日期：2019-01-01 00:00:00

  • A new mathematical model for nitrogen gas production with special emphasis on the role of attached growth media in anammox hybrid reactor.

    abstract：:The present study emphasised on the development of new mathematical models based on mass balance and stoichiometry of nitrogen removal in anammox hybrid reactor (AHR). The performance of AHR at varying hydraulic retention times (HRTs) and nitrogen loading rates (NLRs) revealed that nitrogen removal efficiency (NRE) in...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-015-6793-9

    authors： Tomar S,Gupta SK

    更新日期：2015-11-01 00:00:00

  • Biodegradation of atrazine under denitrifying conditions.

    abstract：:Anaerobic biodegradation of atrazine by the bacterial isolate M91-3 was characterized with respect to mineralization, metabolite formation, and denitrification. The ability of the isolate to enhance atrazine biodegradation in anaerobic sediment slurries was also investigated. The organism utilized atrazine as its sole...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s002530051223

    authors： Crawford JJ,Sims GK,Mulvaney RL,Radosevich M

    更新日期：1998-05-01 00:00:00

  • Identification and biochemical characterization of a novel cold-adapted 1,3-α-3,6-anhydro-L-galactosidase, Ahg786, from Gayadomonas joobiniege G7.

    abstract：:Agar is a major polysaccharide of red algal cells and is mainly decomposed into neoagarobiose by the co-operative effort of β-agarases. Neoagarobiose is hydrolyzed into monomers, D-galactose and 3,6-anhydro-L-galactose, via a microbial oxidative process. Therefore, the enzyme, 1,3-α-3,6-anhydro-L-galactosidase (α-neoa...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-018-9277-x

    authors： Asghar S,Lee CR,Park JS,Chi WJ,Kang DK,Hong SK

    更新日期：2018-10-01 00:00:00

  • Molecular tools for gene manipulation in filamentous fungi.

    abstract：:Functional genomics of filamentous fungi has gradually uncovered gene information for constructing 'cell factories' and controlling pathogens. Available gene manipulation methods of filamentous fungi include random integration methods, gene targeting technology, gene editing with artificial nucleases and RNA technolog...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章,评审

    doi：10.1007/s00253-017-8486-z

    authors： Wang S,Chen H,Tang X,Zhang H,Chen W,Chen YQ

    更新日期：2017-11-01 00:00:00

  • Antimicrobial activity, cytotoxicity and chemical analysis of lemongrass essential oil (Cymbopogon flexuosus) and pure citral.

    abstract：:The aim of this study was to determine the antimicrobial effects of lemongrass essential oil (C. flexuosus) and to determine cytotoxic effects of both test compounds on human dermal fibroblasts. Antimicrobial susceptibility screening was carried out using the disk diffusion method. Antimicrobial resistance was observe...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-016-7807-y

    authors： Adukwu EC,Bowles M,Edwards-Jones V,Bone H

    更新日期：2016-11-01 00:00:00

  • Motility influences biofilm architecture in Escherichia coli.

    abstract：:Eight Escherichia coli strains were studied in minimal medium with a continuous flow system using confocal microscopy. K12 wild-type strains ATCC 25404 and MG1655 formed the best biofilms ( approximately 43 microm thick, 21 to 34% surface coverage). JM109, DH5alpha, and MG1655 motA formed intermediate biofilms ( appro...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-005-0263-8

    authors： Wood TK,González Barrios AF,Herzberg M,Lee J

    更新日期：2006-09-01 00:00:00

  • Molecular analysis of dominant species in Listeria monocytogenes-positive biofilms in the drains of food processing facilities.

    abstract：:Listeria monocytogenes exhibits symbiotic codependence with the dominant commensal bacteria, which may help it avoid being removed or inactivated by disinfectants in local environments. In this study, we investigated L. monocytogenes-positive biofilms at food production facilities, and the dominant bacterial species o...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-015-7203-z

    authors： Liu Y,Zhang H,Wu C,Deng W,Wang D,Zhao G,Song J,Jiang Y

    更新日期：2016-04-01 00:00:00

  • New tool for spreading proteins to the environment: Cry1Ab toxin immobilized to bioplastics.

    abstract：:A new tool to provide an environmentally friendly way to deliver active proteins to the environment has been developed, based on the use of polyhydroxyalkanoate (PHA, bioplastic) granules. To illustrate this novel approach, a derived Cry1Ab insect-specific toxin protein was in vivo immobilized into PHA granules throug...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-005-0257-6

    authors： Moldes C,Farinós GP,de Eugenio LI,García P,García JL,Ortego F,Hernández-Crespo P,Castañera P,Prieto MA

    更新日期：2006-08-01 00:00:00

  • Increased production of pyruvic acid by Escherichia coli RNase G mutants in combination with cra mutations.

    abstract：:The Escherichia coli RNase G is known as an endoribonuclease responsible for the 5'-end maturation of 16S rRNA and degradation of several specific mRNAs such as adhE and eno mRNAs. In this study, we found that an RNase G mutant derived from the MC1061 strain did not grow on a glucose minimal medium. Genetic analysis r...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-007-1006-9

    authors： Sakai T,Nakamura N,Umitsuki G,Nagai K,Wachi M

    更新日期：2007-08-01 00:00:00

  • Production of selenomethionine-labelled proteins using simplified culture conditions and generally applicable host/vector systems.

    abstract：:The amino acid analogue selenomethionine (SeMet) is shown to be efficiently incorporated into recombinant proteins expressed in Escherichia coli grown in a simple minimal medium without the addition of synthetic amino acids. Furthermore, satisfactory SeMet incorporation is obtained with a methionine-prototrophic strai...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s002530100690

    authors： Guerrero SA,Hecht HJ,Hofmann B,Biebl H,Singh M

    更新日期：2001-09-01 00:00:00

  • Application of cell culture enrichment for improving the sensitivity of mycoplasma detection methods based on nucleic acid amplification technology (NAT).

    abstract：:Herein, we present data demonstrating that the application of initial cell culture enrichment could significantly improve mycoplasma testing methods based on the nucleic acid amplification technology (NAT) including a polymerase chain reaction (PCR)/microarray method. The results of the study using Vero cells demonstr...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-007-1135-1

    authors： Kong H,Volokhov DV,George J,Ikonomi P,Chandler D,Anderson C,Chizhikov V

    更新日期：2007-11-01 00:00:00

  • Heterologous expression of the C-terminal antigenic domain of the malaria vaccine candidate Pfs48/45 in the green algae Chlamydomonas reinhardtii.

    abstract：:Malaria is a widespread and infectious disease that is a leading cause of death in many parts of the world. Eradication of malaria has been a major world health goal for decades, but one that still remains elusive. Other diseases have been eradicated using vaccination, but traditional vaccination methods have thus far...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-012-4071-7

    authors： Jones CS,Luong T,Hannon M,Tran M,Gregory JA,Shen Z,Briggs SP,Mayfield SP

    更新日期：2013-03-01 00:00:00

  • Lactobacillus reuteri CRL 1101 highly produces mannitol from sugarcane molasses as carbon source.

    abstract：:Mannitol is a natural polyol extensively used in the food industry as low-calorie sugar being applicable for diabetic food products. We aimed to evaluate mannitol production by Lactobacillus reuteri CRL 1101 using sugarcane molasses as low-cost energy source. Mannitol formation was studied in free-pH batch cultures us...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-012-3945-z

    authors： Ortiz ME,Fornaguera MJ,Raya RR,Mozzi F

    更新日期：2012-08-01 00:00:00

  • Development of a yeast protein fragment complementation assay (PCA) system using dihydrofolate reductase (DHFR) with specific additives.

    abstract：:A yeast protein fragment complementation assay (PCA) system based on dihydrofolate reductase (DHFR) is difficult to be operated because it is not as sensitive to trimethoprim (TMP) as the system using a prokaryotic microorganism. Here, the PCA system using DHFR, specific inhibitors, and a substrate in the yeast Saccha...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-008-1624-x

    authors： Shibasaki S,Sakata K,Ishii J,Kondo A,Ueda M

    更新日期：2008-09-01 00:00:00

  • Chemical and enzymatic routes to dihydroxyacetone phosphate.

    abstract：:Stereoselective carbon-carbon bond formation with aldolases has become an indispensable tool in preparative synthetic chemistry. In particular, the dihydroxyacetone phosphate (DHAP)-dependent aldolases are attractive because four different types are available that allow access to a complete set of diastereomers of vic...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章,评审

    doi：10.1007/s00253-007-0882-3

    authors： Schümperli M,Pellaux R,Panke S

    更新日期：2007-05-01 00:00:00

  • Heterologous expression of the benzoate para-hydroxylase encoding gene (CYP53B1) from Rhodotorula minuta by Yarrowia lipolytica.

    abstract：:There is currently an increasing number of cytochrome P450 (CYP450) monooxygenase encoding genes becoming available from various genome-sequencing projects. These enzymes require association with cytochrome P450 reductase (CPR) to achieve optimal activities. In this study, the CYP53B1 gene, which encodes a benzoate pa...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-005-0264-7

    authors： Shiningavamwe A,Obiero G,Albertyn J,Nicaud JM,Smit M

    更新日期：2006-09-01 00:00:00

  • Intracellular azo decolorization is coupled with aerobic respiration by a Klebsiella oxytoca strain.

    abstract：:Reduction of azo dye methyl red coupled with aerobic respiration by growing cultures of Klebsiella oxytoca GS-4-08 was investigated. In liquid media containing dye and 0.6 % glucose in a mineral salts base, 100 mg l(-1) of the dye are completely removed in 3 h under shaking conditions. The dye cannot be aerobically de...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-014-6161-1

    authors： Yu L,Zhang XY,Xie T,Hu JM,Wang S,Li WW

    更新日期：2015-03-01 00:00:00

  • Efficient multi-enzyme-catalyzed CDP-choline production driven by an ATP donor module.

    abstract：:Cytidine diphosphate choline (CDP-choline) has been applied for treating acute craniocerebral injury and allowing recovery of consciousness after brain surgery. In this study, an acetate kinase (ACK)/acetyl phosphate system was used to supply ATP and combined with Escherichia coli-overexpressed CMP kinase (CMK), NDP k...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-016-7874-0

    authors： Liu Y,Wang J,Xu C,Chen Y,Yang J,Liu D,Niu H,Jiang Y,Yang S,Ying H

    更新日期：2017-02-01 00:00:00

  • Cholesterol oxidase ChoL is a critical enzyme that catalyzes the conversion of diosgenin to 4-ene-3-keto steroids in Streptomyces virginiae IBL-14.

    abstract：:Diosgenin transformation was studied in Streptomyces virginiae IBL-14, a soil-dwelling bacterium with diosgenin-degrading capacity. All of the derivatives isolated were identified as 4-ene-3-keto steroids. We cloned ChoL, a fragment of a cholesterol oxidase from S. virginiae IBL-14, and used gene-disruption techniques...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-009-2188-0

    authors： Li B,Wang W,Wang FQ,Wei DZ

    更新日期：2010-02-01 00:00:00

  • Single-cell protein production from Jerusalem artichoke extract by a recently isolated marine yeast Cryptococcus aureus G7a and its nutritive analysis.

    abstract：:After crude protein of the marine yeast strains maintained in this laboratory was estimated by the method of Kjehldahl, we found that the G7a strain which was identified to be a strain of Cryptococcus aureus according to the routine identification and molecular methods contained high level of protein and could grow on...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-007-1210-7

    authors： Gao L,Chi Z,Sheng J,Ni X,Wang L

    更新日期：2007-12-01 00:00:00

  • Asymmetrical response of anaerobic digestion microbiota to temperature changes.

    abstract：:In natural settings, anaerobic digestion can take place in a wide temperature range, but industrial digesters are usually operated under either mesophilic (~35 °C) or thermophilic (~55 °C) conditions. The ability of anaerobic digestion microbiota to switch from one operating temperature to the other remains poorly doc...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-015-7046-7

    authors： Chapleur O,Mazeas L,Godon JJ,Bouchez T

    更新日期：2016-02-01 00:00:00

  • Screening of high-yielding biocontrol bacterium Bs-916 mutant by ion implantation.

    abstract：:Bacillus subtilis 916 was an effective biocontrol agent in control rice sheath blight caused by Rhizoctonia solani. To further improve its antagonistic ability, low-energy ion implantation was applied in Bs-916. We studied the effects of different doses of N(+) implantation. The optimum dose of ion implantation for th...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-007-0951-7

    authors： Li D,Nie F,Wei L,Wei B,Chen Z

    更新日期：2007-07-01 00:00:00