New tool for spreading proteins to the environment: Cry1Ab toxin immobilized to bioplastics.

abstract：:The addition of humic acid (HA) to polycyclic aromatic hydrocarbon (PAH) contaminated systems has been shown to enhance, inhibit, or have no effect on the biodegradation of these PAHs. In this study, the surfactant effects of Elliott soil HA (ESHA) at two pH values were tested. At pH 7.0, ESHA did not behave as a surf...

journal_title：Applied microbiology and biotechnology

authors： Liang Y,Britt DW,McLean JE,Sorensen DL,Sims RC

更新日期：2007-04-01 00:00:00

abstract：:Chimeric virus-like particles (VLPs) were developed as a candidate for allergen-specific immunotherapy. In this study, hepatitis B core antigen (HBcAg) that genetically fused to Chenopodium album polcalcin (Che a 3)-derived peptide was expressed in E. coli BL21, purified, and VLP formation was evaluated using native a...

journal_title：Applied microbiology and biotechnology

authors： Sani MZ,Bargahi A,Momenzadeh N,Dehghani P,Moghadam MV,Maleki SJ,Nabipour I,Shirkani A,Akhtari J,Hesamizadeh K,Heidari S,Omrani F,Akbarzadeh S,Mohammadi M

更新日期：2021-01-01 00:00:00

abstract：:Streptomyces viridochromogenes Tü494 produces the antibiotic phosphinothricyl-alanyl-alanine (Ptt). Ptt-non-producing mutants were isolated following N-methyl-N'-nitro-N-nitrosoguanidine (NTG) or UV light treatment of spore suspensions. In co-synthesis and bioconversion experiments the mutational blocks in the biosynt...

journal_title：Applied microbiology and biotechnology

authors： Alijah R,Dorendorf J,Talay S,Pühler A,Wohlleben W

更新日期：1991-03-01 00:00:00

abstract：:There is emerging interest in the anaerobic degradation of crude oil. However, there is limited knowledge about the geochemical effects and microbiological activities for it. A mixture of anaerobic sludge and the production water from an oil well was used as an inoculum to construct four consortia, which were incubate...

journal_title：Applied microbiology and biotechnology

authors： Xiong S,Li X,Chen J,Zhao L,Zhang H,Zhang X

更新日期：2015-02-01 00:00:00

abstract：:Microbial natural products, in particular, the ones produced by the members of the order Actinomycetales, will continue to represent an important route to the discovery of novel classes of bioactive compounds. As a result, the search for and discovery of lesser-known and/or novel actinomycetes is of significant intere...

journal_title：Applied microbiology and biotechnology

authors： Kurtböke DI

更新日期：2012-03-01 00:00:00

abstract：:Peroxidases (PRX, EC 1.11.1.7) are widely distributed across microorganisms, plants, and animals; and, in plants, they have been implicated in a variety of secondary metabolic reactions. In particular, horseradish (Armoracia rusticana) root represents the main source of commercial PRX production. The prxC1a gene, whic...

journal_title：Applied microbiology and biotechnology

authors： Matsui T,Nakayama H,Yoshida K,Shinmyo A

更新日期：2003-10-01 00:00:00

abstract：:Bifidobacterium bifidum NCIMB41171 carries four genes encoding different beta-galactosidases. One of them, named bbgIII, consisted of an open reading frame of 1,935 amino acid (a.a.) residues encoding a protein with a multidomain structure, commonly identified on cell wall bound enzymes, having a signal peptide, a mem...

journal_title：Applied microbiology and biotechnology

authors： Goulas TK,Goulas AK,Tzortzis G,Gibson GR

更新日期：2007-10-01 00:00:00

abstract：:The basidiomycete Cystoderma carcharias transformed citronellol into 3,7-dimethyl-1,6,7-octanetriol as the main product. 3,7-Dimethyl-6,7-epoxy-1-octanol was identified as important intermediary product of the biotransformation, and the allylic diols 2,6-dimethyl-2-octene-1,8-diol, 3,7-dimethyl-5-octene-1,7-diol and 3...

journal_title：Applied microbiology and biotechnology

authors： Onken J,Berger RG

更新日期：1999-02-01 00:00:00

abstract：:Biobutanol can be indigenously synthesized by solventogenic Clostridium species; however, these microorganisms possess inferior capability of utilizing abundant and renewable organic wastes, such as starch, lignocellulose, and even syngas. The common strategy to achieve direct butanol production from these organic was...

journal_title：Applied microbiology and biotechnology

authors： Jiang Y,Zhang T,Lu J,Dürre P,Zhang W,Dong W,Zhou J,Jiang M,Xin F

更新日期：2018-07-01 00:00:00

abstract：:In this work, the production of 1,3-propanediol from glucose and molasses was studied in a two-step process using two recombinant microorganisms. The first step of the process is the conversion of glucose or other sugar into glycerol by the metabolic engineered Saccharomyces cerevisiae strain HC42 adapted to high (>20...

journal_title：Applied microbiology and biotechnology

authors： Mendes FS,González-Pajuelo M,Cordier H,François JM,Vasconcelos I

更新日期：2011-11-01 00:00:00

abstract：:Plants as well as pro- and eukaryotic microorganisms are able to synthesise lysine via de novo synthesis. While plants and bacteria, with some exceptions, rely on variations of the meso-diaminopimelate pathway for lysine biosynthesis, fungi exclusively use the α-aminoadipate pathway. Although bacteria and fungi are, i...

journal_title：Applied microbiology and biotechnology

authors： Fazius F,Zaehle C,Brock M

更新日期：2013-05-01 00:00:00

abstract：:Fat, oil and, grease (FOG) deposits are one primary cause of sanitary sewer overflows (SSOs). While numerous studies have examined the formation of FOG deposits in sewer pipes, little is known about their biodegradation under sewer environments. In this study, FOG deposit biodegradation potential was determined by stu...

journal_title：Applied microbiology and biotechnology

authors： He X,Zhang Q,Cooney MJ,Yan T

更新日期：2015-07-01 00:00:00

abstract：:The effect of yeast extract and its less complex substituents on the rate of aerobic dechlorination of 2-chlorobenzoic acid (2-ClBZOH) and 2,5-dichlorobenzoic acid (2,5-Cl2BZOH) by Pseudomonas sp. CPE2 strain, and of 3-chlorobenzoic acid (3-ClBZOH), 4-chlorobenzoic acid (4-ClBZOH) and 3,4-dichlorobenzoic acid (3,4-Cl2...

journal_title：Applied microbiology and biotechnology

authors： Fava F,Armenante PM,Kafkewitz D,Marchetti L

更新日期：1995-04-01 00:00:00

abstract：:Malate synthase (Mls), a key enzyme in the glyoxylate cycle, is required for virulence in microbial pathogens. In this study, we identified the AoMls gene from the nematode-trapping fungus Arthobotrys oligospora. The gene contains 4 introns and encodes a polypeptide of 540 amino acids. To characterize the function of ...

journal_title：Applied microbiology and biotechnology

authors： Zhao X,Wang Y,Zhao Y,Huang Y,Zhang KQ,Yang J

更新日期：2014-03-01 00:00:00

abstract：:Winemaking, brewing and baking are some of the oldest biotechnological processes. In all of them, alcoholic fermentation is the main biotransformation and Saccharomyces cerevisiae the primary microorganism. Although a wide variety of microbial species may participate in alcoholic fermentation and contribute to the sen...

journal_title：Applied microbiology and biotechnology

authors： Albergaria H,Arneborg N

更新日期：2016-03-01 00:00:00

abstract：:Avian influenza virus (AIV) is spreading worldwide and is a serious threat to the health of poultry and humans. In many countries, low pathogenic AIVs, such as H9N2, have become an enormous economic burden on the commercial poultry industry because they cause mild respiratory disease and decrease egg production. A rec...

journal_title：Applied microbiology and biotechnology

authors： Yang WT,Yang GL,Shi SH,Liu YY,Huang HB,Jiang YL,Wang JZ,Shi CW,Jing YB,Wang CF

更新日期：2017-06-01 00:00:00

abstract：:Transformants of the Trichoderma reeseistrains QM9414 and Rut-C30 were constructed in which the genes for the two major hydrophobin proteins, hydrophobins I (HFBI) and II (HFBII), were deleted or amplified by molecular biological techniques. Growth parameters and foam production of the transformant strains were compar...

journal_title：Applied microbiology and biotechnology

authors： Bailey MJ,Askolin S,Hörhammer N,Tenkanen M,Linder M,Penttilä M,Nakari-Setälä T

更新日期：2002-05-01 00:00:00

abstract：:To meet the demand for sustainable energy, aquifer thermal energy storage (ATES) is widely used in the subsurface in urban areas. However, contamination of groundwater, especially with chlorinated volatile organic compounds (CVOCs), is often being encountered. This is commonly seen as an impediment to ATES implementat...

journal_title：Applied microbiology and biotechnology

authors： Ni Z,van Gaans P,Smit M,Rijnaarts H,Grotenhuis T

更新日期：2016-04-01 00:00:00

abstract：:The 16S ribosomal RNA (rRNA) gene has been the most commonly used sequence to characterize bacterial communities. The classical approach to obtain gene sequences to study bacterial diversity implies cloning amplicons, selecting clones, and Sanger sequencing cloned fragments. A more recent approach is direct sequencing...

journal_title：Applied microbiology and biotechnology

authors： Assunção A,Costa MC,Carlier JD

更新日期：2016-03-01 00:00:00

abstract：:Damages of the (agro)ecosystem by extensive use of chemical fertilizers and pesticides, the global dying of bee populations possibly linked to pesticide spraying, and stricter regulations for pesticide use together with successful use of microbials in IPM programs are pushing on the development and commercialization o...

journal_title：Applied microbiology and biotechnology

authors： Preininger C,Sauer U,Bejarano A,Berninger T

更新日期：2018-09-01 00:00:00

abstract：:The environmental pollutant 4-sec-butylphenol (4-sec-BP) which possesses estrogenic properties was transformed by the aerobic Gram-positive bacteria Mycobacterium neoaurum and Nocardia cyriacigeorgica into three main products (P1-P3) which were isolated and structurally characterized in detail. Two of them were produc...

journal_title：Applied microbiology and biotechnology

authors： Hahn V,Sünwoldt K,Mikolasch A,Schauer F

更新日期：2013-09-01 00:00:00

abstract：:Sphingobium sp. strain TCM1 can significantly degrade chlorinated organophosphorus flame retardants, such as tris(2-chloroethyl) phosphate. The PhoK of strain TCM1 (Sb-PhoK) is the main alkaline phosphatase (APase) that catalyzes the last step in the degradation pathway. Here, we purified and characterized Sb-PhoK pro...

journal_title：Applied microbiology and biotechnology

authors： Takahashi S,Morooka Y,Kumakura T,Abe K,Kera Y

更新日期：2020-02-01 00:00:00

abstract：:Smokeless tobacco (ST) products are used worldwide and are a major public health concern. In addition to harmful chemicals found in these products, microbes found in ST products are believed to be responsible for generating harmful tobacco-specific nitrosamines (TSNAs), the most abundant carcinogens in ST. These micro...

journal_title：Applied microbiology and biotechnology

authors： Tyx RE,Rivera AJ,Keong LM,Stanfill SB

更新日期：2020-01-01 00:00:00

abstract：:The tandem multimeric expression of various peptides has been explored by many researchers. However, expression levels have usually not been proportional to the degree of multimerization. To increase the expression level in Escherichia coli of tandem multimers of a cationic antimicrobial peptide, buforin II, fused to ...

journal_title：Applied microbiology and biotechnology

authors： Lee JH,Kim MS,Cho JH,Kim SC

更新日期：2002-05-01 00:00:00

abstract：:Membrane integrity has been used as a criterion for the definition of cell viability. In the present work, staining conditions (time and dye concentration) for the evaluation of membrane integrity in a fluorescence microplate reader, using the membrane-impermeant nucleic-acid dye SYTOX Green, were optimized. Incubatin...

journal_title：Applied microbiology and biotechnology

authors： Machado MD,Soares EV

更新日期：2012-08-01 00:00:00

abstract：:The dawn of a new Proteomics era, just over a decade ago, allowed for large-scale protein profiling studies that have been applied in the identification of distinctive molecular cell signatures. Proteomics provides a powerful approach for identifying and studying these multiple molecular markers in a vast array of bio...

journal_title：Applied microbiology and biotechnology

authors： da Costa JP,Carvalhais V,Ferreira R,Amado F,Vilanova M,Cerca N,Vitorino R

更新日期：2015-09-01 00:00:00

abstract：:The synergistic effect between polyoxometalates (POMs), namely K(5)[SiW(11)V(V)O(40)]·11H(2)O and H(5)[PMo(10)V(V) (2)O(40)]·13H(2)O and laccase from ascomycete Myceliophthora thermophila has been employed for the first time in oxidative polymerization of catechol. Such a laccase-mediator system allowed the formation ...

journal_title：Applied microbiology and biotechnology

authors： Kim S,Silva C,Evtuguin DV,Gamelas JA,Cavaco-Paulo A

更新日期：2011-02-01 00:00:00

abstract：:A Lactobacillus brevis strain with the ability to synthesize butanol from glucose was constructed by metabolic engineering. The genes crt, bcd, etfB, etfA, and hbd, composing the bcs-operon, and the thl gene encode the enzymes of the lower part of the clostridial butanol pathway (crotonase, butyryl-CoA-dehydrogenase, ...

journal_title：Applied microbiology and biotechnology

authors： Berezina OV,Zakharova NV,Brandt A,Yarotsky SV,Schwarz WH,Zverlov VV

更新日期：2010-06-01 00:00:00

abstract：:The existence of taste and odour (T&O) in drinking water is one of the principal causes of consumer complaints and is commonly related to algae growth. Numerous studies have confirmed the existence of algal blooms emerging specifically in low-temperature periods, herein referred to as "cold algae"; these include chrys...

journal_title：Applied microbiology and biotechnology

authors： Cen C,Zhang K,Zhang T,Zhou X,Pan R

更新日期：2020-11-01 00:00:00

abstract：:Agrobacterium tumefaciens has been widely used as a tool for transgenesis in plants. The availability of its genome sequence should facilitate the directed engineering of improved properties; however, the current genome engineering options are laborious. Here, we investigated whether the lambda Red operon can be appli...

journal_title：Applied microbiology and biotechnology

authors： Hu S,Fu J,Huang F,Ding X,Stewart AF,Xia L,Zhang Y

更新日期：2014-03-01 00:00:00