Genome engineering of Agrobacterium tumefaciens using the lambda Red recombination system.

abstract：:The unicellular cyanobacterium Synechocystis sp. PCC6714 can grow not only under photoautotrophic conditions, but also under chemoheterotrophic conditions if glucose is added to the medium. This makes it useful for the study of many aspects of bioenergetic mechanisms. In contrast to its closely related strain Synechoc...

journal_title：Applied microbiology and biotechnology

authors： Ludwig A,Heimbucher T,Gregor W,Czerny T,Schmetterer G

更新日期：2008-03-01 00:00:00

abstract：:The most notable microbial survival models of disinfection kinetics are the original and modified versions of the static Chick-Watson-Hom's (CWH) initially developed for water chlorination. They can all be viewed as special cases of the Weibull survival model, where the observed static curve is the cumulative form (CD...

journal_title：Applied microbiology and biotechnology

authors： Peleg M

更新日期：2021-01-01 00:00:00

abstract：:Biofilms of selected bacteria strains were previously used on metal coupons as a protective layer against microbiologically influenced corrosion of metals. Unlike metal surfaces, concrete surfaces present a hostile environment for growing a protective biofilm. The main objective of this research was to investigate whe...

journal_title：Applied microbiology and biotechnology

authors： Soleimani S,Ormeci B,Isgor OB

更新日期：2013-02-01 00:00:00

abstract：:Linkages among bioreactor operation and performance and microbial community structure were investigated for a fixed-bed biofilm system designed to remove perchlorate from drinking water. Perchlorate removal was monitored to evaluate reactor performance during and after the frequency and intensity of the backwash proce...

journal_title：Applied microbiology and biotechnology

authors： Li X,Yuen W,Morgenroth E,Raskin L

更新日期：2012-11-01 00:00:00

abstract：:DNA polymerase I gene was cloned and sequenced from the thermophilic bacterium Geobacillus caldoxylosilyticus TK4. The gene is 2,634 bp long and encodes a protein of 878 amino acids in length. The enzyme has a molecular mass of 99 kDa and shows sequence homology with DNA polymerase I from Bacillus species (89% identit...

journal_title：Applied microbiology and biotechnology

authors： Sandalli C,Singh K,Modak MJ,Ketkar A,Canakci S,Demir I,Belduz AO

更新日期：2009-08-01 00:00:00

abstract：:The knowledge of biosynthesis gene clusters, production improving methods, and bioactivity mechanisms is very important for the development of filamentous fungi metabolites. Metabolic engineering and heterologous expression methods can be applied to improve desired metabolite production, when their biosynthesis pathwa...

journal_title：Applied microbiology and biotechnology

authors： Yin Y,Cai M,Zhou X,Li Z,Zhang Y

更新日期：2016-09-01 00:00:00

abstract：:Targeted gene mutation by allelic replacement is important for functional genomic analysis and metabolic engineering. However, it is challenging in mutating the essential genes with the traditional method by using a selection marker, since the first step of essential gene knockout will result in a lethal phenotype. He...

journal_title：Applied microbiology and biotechnology

authors： Yang S,Cao X,Yu W,Li S,Zhou YJ

更新日期：2020-04-01 00:00:00

abstract：:In the present study, the modulatory effects of bifidobacterial spp. (Bifidobacterium breve NCIM 5671, Bifidobacterium longum NCIM 5672 and Bifidobacterium bifidum NCIM 5697) on adjuvant induced arthritis in rats were evaluated. Arthritis was induced in male Wistar rats by injecting 250 μg of Freund's adjuvant directl...

journal_title：Applied microbiology and biotechnology

authors： Achi SC,Talahalli RR,Halami PM

更新日期：2019-08-01 00:00:00

abstract：:Bioconversion of cassava-derived glucose to 2-keto-D-gluconic acid (2-KDG) using resting cells of immobilized Pseudomonas aeruginosa IFO 3448 was investigated. The tuberous roots of cassava were selected as the feedstock as they are inexpensive and widely available, and possess high amounts of starch (approximately 70...

journal_title：Applied microbiology and biotechnology

authors： Chia M,Van Nguyen TB,Choi WJ

更新日期：2008-04-01 00:00:00

abstract：:The white button mushroom Agaricus bisporus is economically the most important commercially produced edible fungus. It is grown on carbon- and nitrogen-rich substrates, such as composted cereal straw and animal manure. The commercial mushroom production process is usually performed in buildings or tunnels under highly...

journal_title：Applied microbiology and biotechnology

authors： Kabel MA,Jurak E,Mäkelä MR,de Vries RP

更新日期：2017-06-01 00:00:00

abstract：:The effect of yeast extract and its less complex substituents on the rate of aerobic dechlorination of 2-chlorobenzoic acid (2-ClBZOH) and 2,5-dichlorobenzoic acid (2,5-Cl2BZOH) by Pseudomonas sp. CPE2 strain, and of 3-chlorobenzoic acid (3-ClBZOH), 4-chlorobenzoic acid (4-ClBZOH) and 3,4-dichlorobenzoic acid (3,4-Cl2...

journal_title：Applied microbiology and biotechnology

authors： Fava F,Armenante PM,Kafkewitz D,Marchetti L

更新日期：1995-04-01 00:00:00

abstract：:The dawn of a new Proteomics era, just over a decade ago, allowed for large-scale protein profiling studies that have been applied in the identification of distinctive molecular cell signatures. Proteomics provides a powerful approach for identifying and studying these multiple molecular markers in a vast array of bio...

journal_title：Applied microbiology and biotechnology

authors： da Costa JP,Carvalhais V,Ferreira R,Amado F,Vilanova M,Cerca N,Vitorino R

更新日期：2015-09-01 00:00:00

abstract：:Poly(lactate-co-3-hydroxybutyrate) (P(LA-co-3HB)) was previously produced from xylose in engineered Escherichia coli. The aim of this study was to increase the polymer productivity and LA fraction in P(LA-co-3HB) using two metabolic engineering approaches: (1) deletions of competing pathways to lactate production and ...

journal_title：Applied microbiology and biotechnology

authors： Nduko JM,Matsumoto K,Ooi T,Taguchi S

更新日期：2014-03-01 00:00:00

abstract：:D-lactic acid is used as a monomer in the production of poly-D-lactic acid (PDLA), which is used to form heat-resistant stereocomplex poly-lactic acid. To produce cost-effective D-lactic acid by using all sugars derived from biomass efficiently, xylose-assimilating genes encoding xylose isomerase and xylulokinase were...

journal_title：Applied microbiology and biotechnology

authors： Zhang Y,Vadlani PV,Kumar A,Hardwidge PR,Govind R,Tanaka T,Kondo A

更新日期：2016-01-01 00:00:00

abstract：:The exclusive use of isopropyl beta-D-thiogalactopyranoside to activate the T7 promoter for protein production has limited the general use of the expression system. We have sought an alternative by constructing a recombinant Escherichia coli strain, BL21 (G2), to carry a chromosomal copy of T7 gene 1 fused to the lamb...

journal_title：Applied microbiology and biotechnology

authors： Chao YP,Law W,Chen PT,Hung WB

更新日期：2002-03-01 00:00:00

abstract：:Benzene dioxygenase (BDO; EC 1.14.12.3) from Pseudomonas putida ML2 dihydroxylates benzene to produce cis-1,2-dihydroxy-cyclohexa-3,5-diene. As well as oxidising benzene and toluene, cell-free extracts of Escherichia coli JM109 expressing recombinant BDO oxidised cyclohexene, 1-methylcyclohexene and 3-methylcyclohexen...

journal_title：Applied microbiology and biotechnology

authors： Swift RJ,Carter SF,Widdowson DA,Mason JR,Leak DJ

更新日期：2001-06-01 00:00:00

abstract：:Immobilization has been reported as an efficient technique to address the bacterial vulnerability for application in bio self-healing concrete. In this study, for the first time, magnetic iron oxide nanoparticles (IONs) are being practically employed as the protective vehicle for bacteria to evaluate the self-healing ...

journal_title：Applied microbiology and biotechnology

authors： Seifan M,Sarmah AK,Ebrahiminezhad A,Ghasemi Y,Samani AK,Berenjian A

更新日期：2018-03-01 00:00:00

abstract：:Poly(ɛ-L-lysine) (ɛ-PL) is an unusual biopolymer composed of L-lysine connected between α-carboxyl and ɛ-amino groups. It has been used as a preservative in food and cosmetics industries, drug carrier in medicines, and gene carrier in gene therapy. Modern biotechnology has significantly improved the synthetic efficien...

journal_title：Applied microbiology and biotechnology

authors： Xu Z,Xu Z,Feng X,Xu D,Liang J,Xu H

更新日期：2016-08-01 00:00:00

abstract：:Pimaricin (natamycin) is a small polyene macrolide antibiotic used worldwide. This efficient antimycotic and antiprotozoal agent, produced by several soil bacterial species of the genus Streptomyces, has found application in human therapy, in the food and beverage industries and as pesticide. It displays a broad spect...

journal_title：Applied microbiology and biotechnology

authors： Aparicio JF,Barreales EG,Payero TD,Vicente CM,de Pedro A,Santos-Aberturas J

更新日期：2016-01-01 00:00:00

abstract：:Bovine mastitis results in billion dollar losses annually in the USA alone. Streptococci are among the most relevant causative agents of this disease. Conventional antibiotic therapy is often unsuccessful and contributes to development of antibiotic resistance. Bacteriophage endolysins represent a new class of antimic...

journal_title：Applied microbiology and biotechnology

authors： Schmelcher M,Powell AM,Camp MJ,Pohl CS,Donovan DM

更新日期：2015-10-01 00:00:00

abstract：:The osmotic tolerance of cells of Saccharomyces cerevisiae as a function of glycerol concentration and temperature has been investigated. Results show that under isothermal conditions (25 degrees C) cells are resistant (94% viability) to hyperosmotic treatment at 49.2 MPa. A thigher osmotic pressure, cell viability de...

journal_title：Applied microbiology and biotechnology

authors： Beney L,Marechal PA,Gervais P

更新日期：2001-08-01 00:00:00

abstract：:This study is an application of an experimental design methodology for the optimization of the culture conditions of alpha-L: -arabinofuranosidase production by Talaromyces thermophilus. Wheat bran and yeast extract were first selected as the best carbon and nitrogen sources, respectively, for enzyme production. A Pla...

journal_title：Applied microbiology and biotechnology

authors： Guerfali M,Chaabouni M,Gargouri A,Belghith H

更新日期：2010-02-01 00:00:00

abstract：:The aim of this study was to elucidate the kinetic constraints during the redox biotransformation of the azo dye, Reactive Red 2 (RR2), and carbon tetrachloride (CT) mediated by soluble humic acids (HAs) and immobilized humic acids (HAi), as well as by the quinoid model compounds, anthraquinone-2,6-disulfonate (AQDS) ...

journal_title：Applied microbiology and biotechnology

authors： Cervantes FJ,Martínez CM,Gonzalez-Estrella J,Márquez A,Arriaga S

更新日期：2013-03-01 00:00:00

abstract：:Human immunodeficiency virus (HIV)/AIDS continues to spread worldwide, and most of the HIV-infected people living in developing countries have little or no access to highly active antiretroviral therapy. The development of efficient and low-cost microbicides to prevent sexual transmission of HIV should be given high p...

journal_title：Applied microbiology and biotechnology

authors： Xiong S,Fan J,Kitazato K

更新日期：2010-04-01 00:00:00

abstract：:The transport of glucose by the solventogenic anaerobe Clostridium acetobutylicum was investigated. Glucose phosphoenolpyruvate (PEP)-dependent phosphotransferase system (PTS) activity was detected in extracts prepared from cultures grown on glucose and extract fractionation revealed that both soluble and membrane com...

journal_title：Applied microbiology and biotechnology

authors： Tangney M,Mitchell WJ

更新日期：2007-02-01 00:00:00

abstract：:Interleukin-2 (IL-2) is one of the most successful cytokines applied in tumor immunotherapy because of its ability to stimulate potent cellular immune response. The life-threatening toxicity of vascular leak syndrome (VLS) associated with the high-dose IL-2 treatment regimen has limited its use in tumor immunotherapy....

journal_title：Applied microbiology and biotechnology

authors： Ye L,Fan J,Shi X,Tao Q,Ye D,Xian Z,Zeng X,Li Y,Feng M,Ju D

更新日期：2014-05-01 00:00:00

abstract：:Seed-based expression system is an attractive platform for the production of recombinant proteins in molecular farming. Despite the many advantages of molecular farming, little is known about the effect of the different subcellular accumulation of recombinant proteins on the endoplasmic reticulum (ER) quality control ...

journal_title：Applied microbiology and biotechnology

authors： Wang D,Ma J,Sun D,Li H,Jiang C,Li X

更新日期：2015-08-01 00:00:00

abstract：:In industry, filamentous fungi have a prominent position as producers of economically relevant primary or secondary metabolites. Particularly, the advent of genetic engineering of filamentous fungi has led to a growing number of molecular tools to adopt filamentous fungi for biotechnical applications. Here, we summari...

journal_title：Applied microbiology and biotechnology

authors： Kluge J,Terfehr D,Kück U

更新日期：2018-08-01 00:00:00

abstract：:Physico-chemical parameters, hydrological conditions, and microbial interactions can affect the growth and persistence of cyanobacteria, but the interacting effects among these bloom-forming factors are still poorly known. This hampers our capacity to predict the occurrence of cyanobacterial bloom accurately. Here, we...

journal_title：Applied microbiology and biotechnology

authors： Song H,Xu J,Lavoie M,Fan X,Liu G,Sun L,Fu Z,Qian H

更新日期：2017-02-01 00:00:00

abstract：:Microbial natural products, in particular, the ones produced by the members of the order Actinomycetales, will continue to represent an important route to the discovery of novel classes of bioactive compounds. As a result, the search for and discovery of lesser-known and/or novel actinomycetes is of significant intere...

journal_title：Applied microbiology and biotechnology

authors： Kurtböke DI

更新日期：2012-03-01 00:00:00