Transformation and gene replacement in the facultatively chemoheterotrophic, unicellular cyanobacterium Synechocystis sp. PCC6714 by electroporation.

abstract：:Carvone, the principal component of spearmint oil, induces biodegradation of polychlorinated biphenyls (PCB) by Arthrobacter sp. strain B1B. This study investigated the effectiveness of the repeated application of carvone-induced bacteria for bioremediation of Aroclor-1242-contaminated soil. Control treatments compare...

journal_title：Applied microbiology and biotechnology

authors： Gilbert ES,Crowley DE

更新日期：1998-10-01 00:00:00

abstract：:The efficient production of recombinant proteins such as antibodies typically involves the screening of an extravagant number of clones in order to finally select a stable and high-producing cell line. Thereby, the underlying principles of a powerful protein machinery, but also potential expression limitations, often ...

journal_title：Applied microbiology and biotechnology

authors： Reinhart D,Sommeregger W,Debreczeny M,Gludovacz E,Kunert R

更新日期：2014-07-01 00:00:00

abstract：:The araA gene encoding an L-arabinose isomerase (L-AI) from the acido-thermophilic bacterium Acidothermus cellulolytics ATCC 43068 was cloned and overexpressed in Escherichia coli. The open reading frame of the L-AI consisted of 1,503 nucleotides encoding 501 amino acid residues. The recombinant L-AI was purified to h...

journal_title：Applied microbiology and biotechnology

authors： Cheng L,Mu W,Zhang T,Jiang B

更新日期：2010-04-01 00:00:00

abstract：:Since the last 20 years, bacteria of the genus Acinetobacter have been the leading cause of hospital-acquired infections. In addition to the ability of Acinetobacter species to acquire rapid antibiotic resistance, limited knowledge on the mechanisms of multidrug resistance to antibiotics limits the treatment options f...

journal_title：Applied microbiology and biotechnology

authors： Shin B,Park C,Park W

更新日期：2020-02-01 00:00:00

abstract：:Rapid sugar consumption is important for the microbial production of chemicals and fuels. Here, we show that overexpression of the NADH dehydrogenase gene (ndh) increased glucose consumption rate in Corynebacterium glutamicum under oxygen-deprived conditions through investigating the relationship between the glucose c...

journal_title：Applied microbiology and biotechnology

authors： Tsuge Y,Uematsu K,Yamamoto S,Suda M,Yukawa H,Inui M

更新日期：2015-07-01 00:00:00

abstract：:The obligate photoautotrophic cyanobacterium Synechococcus PCC7942 and the photoheterotrophic heterocystous cyanobacterium Noctoc muscorum are able to reduce prochiral ketones asymmetrically to optical pure chiral alcohols without light. An example is the synthesis of S-pentafluoro(phenyl-)ethanol with an enantiomeric...

journal_title：Applied microbiology and biotechnology

authors： Havel J,Weuster-Botz D

更新日期：2007-07-01 00:00:00

abstract：:A competitive immunosensor using a monoclonal antibody has been developed for the enumeration of Nitrobacter in activated sludge and other environmental samples. Its cross-reactivity was tested against a number of bacterial strains and isolates. All strains of the nitrite-oxidising genera Nitrobacter and Nitrococcus r...

journal_title：Applied microbiology and biotechnology

authors： Sandén B,Dalhammar G

更新日期：2000-09-01 00:00:00

abstract：:Immobilization of the Geotrichum candidum (CCT 1205) cell with functionalized silica creates promising biocatalysts for production of ɛ-caprolactone. The results obtained by immobilization of the whole cell on SiO2-NH2 and SiO2-SH supports indicate that the presence of reactive functional groups on the support may pro...

journal_title：Applied microbiology and biotechnology

authors： Silva ALP,da Silva Caridade TN,Magalhães RR,de Sousa KT,de Sousa CC,Vale JA

更新日期：2020-10-01 00:00:00

abstract：:Conductive materials can facilitate syntrophic methane (CH4) production by improving direct interspecies electron transfer. The effect of a conductive material, ferroferric oxide (Fe3O4), on the anaerobic treatment of tryptone-based high-strength wastewater was investigated in two anaerobic sequencing batch reactors (...

journal_title：Applied microbiology and biotechnology

authors： Yin Q,He K,Liu A,Wu G

更新日期：2017-05-01 00:00:00

abstract：:Aspartic proteases are a relatively small group of proteolytic enzymes that are active in acidic environments and are found across all forms of life. Certain microorganisms secrete such proteases as virulence agents and/or in order to break down proteins thereby liberating assimilable sources of nitrogen. Some of the ...

journal_title：Applied microbiology and biotechnology

authors： Theron LW,Divol B

更新日期：2014-11-01 00:00:00

abstract：:The synthesis of rhamnosylated compounds has gained great importance since these compounds have potential therapeutic applications. The enzymatic approaches for glycosylation of bioactive molecules have been well developed; however, the enzymatic rhamnosylation has been largely hindered by lacking of the glycosyl dono...

journal_title：Applied microbiology and biotechnology

authors： Xu L,Liu X,Li Y,Yin Z,Jin L,Lu L,Qu J,Xiao M

更新日期：2019-10-01 00:00:00

abstract：:Bacterially induced precipitation of minerals leading to cementation of natural geological formations has been well recorded in a variety of environments. A range of microbial pathways and geochemical processes have been found to influence the cementation processes; but detailed formation mechanisms and biogeochemical...

journal_title：Applied microbiology and biotechnology

authors： Ramachandran AL,Polat P,Mukherjee A,Dhami NK

更新日期：2020-04-01 00:00:00

abstract：:Trypsin is commonly used in Madin-Darby canine kidney (MDCK) cell culture-based influenza vaccine production to facilitate virus infection by proteolytic activation of viral haemagglutinin, which enables multi-cycle replication. In this study, we were able to demonstrate that trypsin also interferes with pathogen defe...

journal_title：Applied microbiology and biotechnology

authors： Seitz C,Isken B,Heynisch B,Rettkowski M,Frensing T,Reichl U

更新日期：2012-01-01 00:00:00

abstract：:A simple and stable immobilization of a laccase from Pleurotus ostreatus was obtained through genetic fusion with a self-assembling and adhesive class I hydrophobin. The chimera protein was expressed in Pichia pastoris and secreted into the culture medium. The crude culture supernatant was directly used for coatings o...

journal_title：Applied microbiology and biotechnology

authors： Sorrentino I,Giardina P,Piscitelli A

更新日期：2019-04-01 00:00:00

abstract：:For continuous production of the antibiotic nikkomycin immobilized cells have been used in a fluidized bed bioreactor. Cells of Streptomyces tendae were immobilized on sintered glass particles. Different biomass concentrations on the particles correspond to different thicknesses of mycelial layers because growth occur...

journal_title：Applied microbiology and biotechnology

authors： Trück HU,Chmiel H,Hammes WP,Trösch W

更新日期：1990-10-01 00:00:00

abstract：:This paper studies the initial stages of iridescent tarnishes on titanium heat exchanger tubes in contact with running freshwater on the river Tagus in Spain for up to 20 months. Electrochemical impedance spectroscopy (EIS), scanning electron microscopy [(SEM with energy dispersive X-ray (EDX)] and X-ray photoelectron...

journal_title：Applied microbiology and biotechnology

authors： Moreno DA,Cano E,Ibars JR,Polo JL,Montero F,Bastidas JM

更新日期：2004-05-01 00:00:00

abstract：:Chemical mutagenesis of the phototrophic green microalga Parietochloris incisa, producing high amounts of arachidonic acid (ARA), resulted in selection of a mutant deficient in ARA and rich in dihomo-γ-linolenic acid (DGLA) and thus ∆5 desaturase defective. The mutagenesis produced a nonsense mutation in the ∆5 desatu...

journal_title：Applied microbiology and biotechnology

authors： Iskandarov U,Khozin-Goldberg I,Cohen Z

更新日期：2011-04-01 00:00:00

abstract：:The genomic DNA and cDNA for a gene encoding a novel trehalose synthase (TSase) catalyzing trehalose synthesis from alpha-D-glucose 1-phosphate and D-glucose were cloned from a basidiomycete, Grifola frondosa. Nucleotide sequencing showed that the 732-amino-acid TSase-encoding region was separated by eight introns. Co...

journal_title：Applied microbiology and biotechnology

authors： Saito K,Yamazaki H,Ohnishi Y,Fujimoto S,Takahashi E,Horinouchi S

更新日期：1998-08-01 00:00:00

abstract：:One of the major aims of bioprocess engineering is the real-time monitoring of important process variables. This is the basis of precise process control and is essential for high productivity as well as the exact documentation of the overall production process. Infrared spectroscopy is a powerful analytical technique ...

journal_title：Applied microbiology and biotechnology

authors： Landgrebe D,Haake C,Höpfner T,Beutel S,Hitzmann B,Scheper T,Rhiel M,Reardon KF

更新日期：2010-09-01 00:00:00

abstract：:Population dynamics was studied in a 52-l biotrickling filter (BTF) operated for 182 days and used to clean air contaminated with styrene vapors. In the BTF, biomass grew either as free-floating (planktonic) or attached (sessile) microorganisms. PCR-amplified 16S rDNA fragments from planktonic and sessile cells within...

journal_title：Applied microbiology and biotechnology

authors： Tresse O,Lorrain MJ,Rho D

更新日期：2002-08-01 00:00:00

abstract：:A moderately halophilic alkalitolerant Bacillus sp. Strain TSCVKK, with an ability to produce extracellular halophilic, alkalitolerant, surfactant, and detergent-stable alpha-amylase was isolated from soil samples obtained from a salt-manufacturing industry in Chennai. The culture conditions for higher amylase product...

journal_title：Applied microbiology and biotechnology

authors： Kiran KK,Chandra TS

更新日期：2008-01-01 00:00:00

abstract：:Cell-free preparations of Tetrahymena thermophila catalyze the direct desaturation of cholesterol to delta7-dehydrocholesterol (provitamin D3). The activity was isolated in the microsomal fraction from Tetrahymena homogenates. Delta7-desaturase activity was stimulated fivefold by the addition of 6 mM ATP. Other cofact...

journal_title：Applied microbiology and biotechnology

authors： Valcarce G,Florin-Christensen J,Nudel C

更新日期：2000-05-01 00:00:00

abstract：:In microorganisms, the enzyme acetate kinase (AK) catalyses the formation of ATP from ADP by de-phosphorylation of acetyl phosphate into acetic acid. A mutant strain of Clostridium acetobutylicum lacking acetate kinase activity is expected to have reduced acetate and acetone production compared to the wild type. In th...

journal_title：Applied microbiology and biotechnology

authors： Kuit W,Minton NP,López-Contreras AM,Eggink G

更新日期：2012-05-01 00:00:00

abstract：:The present work created an esterase variant from Rhodobacter sphaeroides (RspE) with enhanced selectivity in hydrolytic kinetic resolutions by directed evolution. A "model" substrate, methyl mandelate, was introduced in the high-throughput screening procedure. E values of a variant CH (Asn62Cys/Leu145His) for six dif...

journal_title：Applied microbiology and biotechnology

authors： Ma J,Wu L,Guo F,Gu J,Tang X,Jiang L,Liu J,Zhou J,Yu H

更新日期：2013-06-01 00:00:00

abstract：:A recombinant oleate hydratase from Lysinibacillus fusiformis converted ricinoleic acid to a product, whose chemical structure was identified as the novel compound 10,12-dihydroxystearic acid by gas chromatograph/mass spectrometry, Fourier transform infrared, and nuclear magnetic resonance analysis. The reaction condi...

journal_title：Applied microbiology and biotechnology

authors： Seo MH,Kim KR,Oh DK

更新日期：2013-10-01 00:00:00

abstract：:Inactivation of D-amino acid oxidase occurred by different mechanisms. The enzyme showed a rapid loss of activity in the presence of micromolar amounts of Cu2+ and Hg2+. It was also sensitive to oxidative inactivation by Fe2+ and H2O2 when both reagents were added in millimolar amounts. When oxidatively inactivated D-...

journal_title：Applied microbiology and biotechnology

authors： Schräder T,Andreesen JR

更新日期：1996-05-01 00:00:00

abstract：:The ability of taxonomically different yeasts to synthesize pyruvic acid (PA) from glucose was studied. The study showed that many yeasts are able to produce PA from glucose under the condition of growth limitation by thiamine. This ability was found in the yeast Blastobotrys adeninivorans for the first time. The prod...

journal_title：Applied microbiology and biotechnology

authors： Kamzolova SV,Morgunov IG

更新日期：2016-09-01 00:00:00

abstract：:Dengue viruses are emerging mosquito-borne pathogens belonging to Flaviviridae family which are transmitted to humans via the bites of infected mosquitoes Aedes aegypti and Aedes albopictus. Because of the wide distribution of these mosquito vectors, more than 2.5 billion people are approximately at risk of dengue inf...

journal_title：Applied microbiology and biotechnology

authors： Fahimi H,Mohammadipour M,Haddad Kashani H,Parvini F,Sadeghizadeh M

更新日期：2018-04-01 00:00:00

abstract：:Pseudomonads are ubiquitous Gram-negative gamma proteobacteria known for their extreme versatility and adaptability. Some are plant pathogens (Pseudomonas syringae) which have to survive on the surface of leaves while others can colonize the rhizosphere or survive in soil (Pseudomonas fluorescens, Pseudomonas putida),...

journal_title：Applied microbiology and biotechnology

authors： Cornelis P

更新日期：2010-05-01 00:00:00

abstract：:The influence of different amino acid substitutions in the nitrilase from Pseudomonas fluorescens EBC191 (NitA) on the catalytical activity and the ability to form amides was investigated. The enzyme variant Glu137Ala was constructed because glutamate residues homologous to Glu137 are highly conserved among different ...

journal_title：Applied microbiology and biotechnology

authors： Sosedov O,Stolz A

更新日期：2015-03-01 00:00:00