Transformation and gene replacement in the facultatively chemoheterotrophic, unicellular cyanobacterium Synechocystis sp. PCC6714 by electroporation.

Abstract:

:The unicellular cyanobacterium Synechocystis sp. PCC6714 can grow not only under photoautotrophic conditions, but also under chemoheterotrophic conditions if glucose is added to the medium. This makes it useful for the study of many aspects of bioenergetic mechanisms. In contrast to its closely related strain Synechocystis sp. PCC6803, which cannot grow chemoheterotrophically, Synechocystis PCC6714 is not naturally transformable. To enable gene transfer in this strain, we established a method for the introduction of self-replicating IncQ plasmids and for gene replacement using electroporation.

authors

Ludwig A,Heimbucher T,Gregor W,Czerny T,Schmetterer G

doi

10.1007/s00253-008-1356-y

subject

Has Abstract

pub_date

2008-03-01 00:00:00

pages

729-35

issue

4

eissn

0175-7598

issn

1432-0614

journal_volume

78

pub_type

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