Abstract：
:Linkages among bioreactor operation and performance and microbial community structure were investigated for a fixed-bed biofilm system designed to remove perchlorate from drinking water. Perchlorate removal was monitored to evaluate reactor performance during and after the frequency and intensity of the backwash procedure were changed, while the microbial community structure was studied using clone libraries and quantitative PCR targeting the 16S rRNA gene. When backwash frequency was increased from once per month to once per day, perchlorate removal initially deteriorated and then recovered, and the relative abundance of perchlorate-reducing bacteria (PRB) initially increased and then decreased. This apparent discrepancy suggested that bacterial populations other than PRB played an indirect role in perchlorate removal, likely by consuming dissolved oxygen, a competing electron acceptor. When backwash intensity was increased, the reactor gradually lost its ability to remove perchlorate, and concurrently the relative abundance of PRB decreased. The results indicated that changes in reactor operation had a profound impact on reactor performance through altering the microbial community structure. Backwashing is an important yet poorly characterized procedure when operating fixed-bed biofilm reactors. Compared to backwash intensity, changes in backwash frequency exerted less disturbance on the microbial community in the current study. If this finding can be confirmed in future work, backwash frequency may serve as the primary parameter when optimizing backwash procedures.
journal_name
Appl Microbiol Biotechnoljournal_title
Applied microbiology and biotechnologyauthors
Li X,Yuen W,Morgenroth E,Raskin Ldoi
10.1007/s00253-011-3838-6subject
Has Abstractpub_date
2012-11-01 00:00:00pages
815-27issue
3eissn
0175-7598issn
1432-0614journal_volume
96pub_type
杂志文章abstract：:The exclusive use of isopropyl beta-D-thiogalactopyranoside to activate the T7 promoter for protein production has limited the general use of the expression system. We have sought an alternative by constructing a recombinant Escherichia coli strain, BL21 (G2), to carry a chromosomal copy of T7 gene 1 fused to the lamb...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
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更新日期：2002-03-01 00:00:00
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journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
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更新日期：2004-06-01 00:00:00
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更新日期：2018-06-01 00:00:00
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pub_type： 杂志文章,评审
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abstract：:In the Funding section, the following statement is missing: The MACS cohort study was supported by the NIH, National Institute of Allergy and Infectious Diseases grant U01-AI35041. ...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章,已发布勘误
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更新日期：2019-07-01 00:00:00
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journal_title：Applied microbiology and biotechnology
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更新日期：2008-01-01 00:00:00
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更新日期：2010-05-01 00:00:00
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journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s002530051325
更新日期：1998-10-01 00:00:00
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journal_title：Applied microbiology and biotechnology
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doi：10.1007/s002530051074
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pub_type： 杂志文章,评审
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journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
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更新日期：1990-12-01 00:00:00
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