Biophysical characterization of the Lactobacillus delbrueckii subsp. bulgaricus membrane during cold and osmotic stress and its relevance for cryopreservation.

abstract：:The transfer of plasmids was studied in a stirred fermentor in the course of mixed batch cultures combining recombinant strains of Lactococcus lactis subsp. lactis (donor strains) with L. lactis subsp. lactis CNRZ 268M3 (recipient strain). Donor strains contained one or two of the following plasmids (coding for erythr...

journal_title：Applied microbiology and biotechnology

authors： el Alami N,Boquien CY,Corrieu G

更新日期：1992-06-01 00:00:00

abstract：:Betalamic acid is the structural unit of all the natural pigments betalains. These are nitrogen-containing water-soluble compounds with high colorant and bioactive properties, characteristic of plants of the order Caryophyllales. The formation of betalamic acid from the precursor amino acid 3,4-dihydroxy-L-phenylalani...

journal_title：Applied microbiology and biotechnology

authors： Gandía-Herrero F,García-Carmona F

更新日期：2014-02-01 00:00:00

abstract：:Blowfly (Diptera: Calliphoridae) species Lucilia sericata (Meigen) and related species Lucilia cuprina (Wiedmann) are important agricultural pests, assist in forensic fields and also have a therapeutic role in medicine. Both species (though predominantly L. sericata) are utilised in a clinical setting for maggot debri...

journal_title：Applied microbiology and biotechnology

authors： Gasz NE,Geary MJ,Doggett SL,Harvey ML

更新日期：2021-01-07 00:00:00

abstract：:Discovery of keratin-degrading enzymes from fungi and bacteria has primarily focused on finding one protease with efficient keratinase activity. Recently, an investigation was conducted of all keratinases secreted from a fungus known to grow on keratinaceous materials, such as feather, horn, and hooves. The study demo...

journal_title：Applied microbiology and biotechnology

authors： Lange L,Huang Y,Busk PK

更新日期：2016-03-01 00:00:00

abstract：:Cyanobacterial mutants defective in acyl-acyl carrier protein synthetase (Aas) secrete free fatty acids (FFAs) into the external medium and hence have been used for the studies aimed at photosynthetic production of biofuels. While the wild-type strain of Synechocystis sp. PCC 6803 is highly sensitive to exogenously ad...

journal_title：Applied microbiology and biotechnology

authors： Kojima K,Keta S,Uesaka K,Kato A,Takatani N,Ihara K,Omata T,Aichi M

更新日期：2016-12-01 00:00:00

abstract：:Actinobacteria, a large group of Gram-positive bacteria, secrete a wide range of extracellular enzymes involved in the degradation of organic compounds and biopolymers including the ubiquitous aminopolysaccharides chitin and chitosan. While chitinolytic enzymes are distributed in all kingdoms of life, actinobacteria a...

journal_title：Applied microbiology and biotechnology

authors： Lacombe-Harvey MÈ,Brzezinski R,Beaulieu C

更新日期：2018-09-01 00:00:00

abstract：:The exclusive use of isopropyl beta-D-thiogalactopyranoside to activate the T7 promoter for protein production has limited the general use of the expression system. We have sought an alternative by constructing a recombinant Escherichia coli strain, BL21 (G2), to carry a chromosomal copy of T7 gene 1 fused to the lamb...

journal_title：Applied microbiology and biotechnology

authors： Chao YP,Law W,Chen PT,Hung WB

更新日期：2002-03-01 00:00:00

abstract：:Catharanthus roseus (L.) G. Don is a plant species known for its production of a variety of terpenoid indole alkaloids, many of which have pharmacological activities. Production of catharanthine in cell cultures or in hairy roots established by transformation with Agrobacterium rhizogenes is of interest because cathar...

journal_title：Applied microbiology and biotechnology

authors： Zhou ML,Hou HL,Zhu XM,Shao JR,Wu YM,Tang YX

更新日期：2011-08-01 00:00:00

abstract：:A cDNA of a structural gene encoding pyranose 2-oxidase (P2O) from Trametes ochracea strain MB49 was cloned into Escherichia coli strain BL21(DE3) on a multicopy plasmid under the control of the trc promoter. Synthesis of P2O was studied in batch cultures in LB or M9-based mineral medium at 28 degrees C. While there w...

journal_title：Applied microbiology and biotechnology

authors： Vecerek B,Maresová H,Kocanová M,Kyslík P

更新日期：2004-05-01 00:00:00

abstract：:Ethidium monoazide bromide (EMA) treatment of pure culture and environmental waters at low concentrations (1.0-7.5 microg/ml) indicated effective enumeration of viable and viable but nonculturable Escherichia coli in pure cultures, creek waters, and secondary activated sludge effluent samples by quantitative polymeras...

journal_title：Applied microbiology and biotechnology

authors： Gedalanga PB,Olson BH

更新日期：2009-03-01 00:00:00

abstract：:Two bacterial strains, Ralstonia sp. LD35 and Pseudomonas putida DSM 1868, were assayed for their ability to degrade the monocyclic aromatic compounds commonly found in olive mill wastewaters (OMWs). The goal was to study the possibility of employing the two strains in the removal of these recalcitrant and toxic compo...

journal_title：Applied microbiology and biotechnology

authors： Di Gioia D,Fava F,Bertin L,Marchetti L

更新日期：2001-05-01 00:00:00

abstract：:Oils and fats are the most important renewable raw materials of the chemical industry. They make available fatty acids in such purity that they may be used for chemical conversions and for the synthesis of chemically pure compounds. Oleic acid (1) from "new sunflower," linoleic acid (2) from soybean, linolenic acid (3...

journal_title：Applied microbiology and biotechnology

authors： Metzger JO,Bornscheuer U

更新日期：2006-06-01 00:00:00

abstract：:The capability of lactic acid bacteria (LAB) to produce exopoly- and oligosaccharides was and is the subject of expanding research efforts. Due to their physicochemical properties and health-promoting potential, exopoly- and oligosaccharides from food-grade LAB can be used in the food and other industries and may have...

journal_title：Applied microbiology and biotechnology

authors： Korakli M,Vogel RF

更新日期：2006-08-01 00:00:00

abstract：:The pathogenicity of Salmonella Typhimurium, a foodborne pathogen, is mainly attributed to its ability to form biofilm on food contact surfaces. ε-polylysine, a polymer of positively charged lysine, is reported to inhibit biofilm formation of both gram-positive and gram-negative bacteria. To elucidate the mechanism un...

journal_title：Applied microbiology and biotechnology

authors： Shen C,Islam MT,Masuda Y,Honjoh KI,Miyamoto T

更新日期：2020-06-01 00:00:00

abstract：:The influence of low temperature (5-29 degrees C) on the methanogenic activity of non-adapted digested sewage sludge and on temperature/leachate-adapted biomass was assayed by using municipal landfill leachate, intermediates of anaerobic degradation (propionate) and methane precursors (acetate, H2/CO2) as substrates. ...

journal_title：Applied microbiology and biotechnology

authors： Kettunen RH,Rintala JA

更新日期：1997-10-01 00:00:00

abstract：:There is a worldwide trend to understand the impact of non-Saccharomyces yeast species on the process of winemaking. Although the predominant species at the end of the fermentation is Saccharomyces cerevisiae, several non-Saccharomyces species present during the first days of the process can produce and/or release aro...

journal_title：Applied microbiology and biotechnology

authors： Barquet M,Martín V,Medina K,Pérez G,Carrau F,Gaggero C

更新日期：2012-01-01 00:00:00

abstract：:A new highly scalable microbial fuel cell (MFC) design, consisting of a series of cassette electrodes (CE), was examined for increasing power production from organic matter in wastewater. Each CE chamber was composed of a box-shaped flat cathode (two air cathodes on both sides) sandwiched in between two proton-exchang...

journal_title：Applied microbiology and biotechnology

authors： Shimoyama T,Komukai S,Yamazawa A,Ueno Y,Logan BE,Watanabe K

更新日期：2008-08-01 00:00:00

abstract：:Polyketides, such as erythromycin, are complex natural products with diverse therapeutic applications. They are synthesized by multi-modular megaenzymes, so-called polyketide synthases (PKSs). The macrolide core of erythromycin, 6-deoxyerythronolide B (6dEB), is produced by the deoxyerythronolide B synthase (DEBS) tha...

journal_title：Applied microbiology and biotechnology

authors： Kumpfmüller J,Methling K,Fang L,Pfeifer BA,Lalk M,Schweder T

更新日期：2016-02-01 00:00:00

abstract：:Heparin is a highly sulfated polysaccharide which belongs to the family of glycosaminoglycans. It is involved in various important biological activities. The major biological purpose is the inhibition of the coagulation cascade to maintain the blood flow in the vasculature. These properties are employed in several the...

journal_title：Applied microbiology and biotechnology

authors： Bolten SN,Rinas U,Scheper T

更新日期：2018-10-01 00:00:00

abstract：:A new pmoA gene-based PCR primer set was designed for detection of nitrite-dependent anaerobic oxidation of methane (n-damo) bacteria from four different ecosystems, namely rice paddy soil, freshwater reservoir, reed bed, and sludge from wastewater treatment plant. This primer set showed high specificity and efficienc...

journal_title：Applied microbiology and biotechnology

authors： Han P,Gu JD

更新日期：2013-12-01 00:00:00

abstract：:Production of bacterial cellulose (BC) using sugar cane molasses (MO) with/without the presence of lignosulfonate (MOL) as a sole carbon source in a Hestrin-Schramm medium (HS) was investigated. Six strains of Acetobacter xylinum [American Type Culture Collection 10245 and Institute of Fermentation in Osaka (IFO) 1369...

journal_title：Applied microbiology and biotechnology

authors： Keshk S,Sameshima K

更新日期：2006-09-01 00:00:00

abstract：:Due to variations in the production levels, a full-scale sequencing batch reactor (SBR) for post-treatment of tannery wastewater was exposed to low and high ammonia load periods. In order to study how these changes affected the N-removal capacity, the microbiology of the reactor was studied by a diverse set of techniq...

journal_title：Applied microbiology and biotechnology

authors： Cabezas A,Draper P,Etchebehere C

更新日期：2009-10-01 00:00:00

abstract：:In order to understand the intracellular responses in recombinant CHO (rCHO) cells producing antibody in serum-free medium (SFM) supplemented with optimized hydrolysates mixtures, yielding the highest specific growth rate (μ, SFM#S1) or the highest specific antibody productivity (q(Ab,) SFM#S2), differentially express...

journal_title：Applied microbiology and biotechnology

authors： Kim JY,Kim YG,Han YK,Choi HS,Kim YH,Lee GM

更新日期：2011-03-01 00:00:00

abstract：:Chitooligosaccharides have important application value in the fields of food and agriculture. Chitosanase can degrade chitosan to obtain chitooligosaccharides. The marine metagenome contains many genes related to the degradation of chitosan. However, it is difficult to mine valuable genes from large gene resources. Th...

journal_title：Applied microbiology and biotechnology

authors： Han Y,Guan F,Sun J,Wu N,Tian J

更新日期：2020-08-01 00:00:00

abstract：:Chemically synthesized 2-hydroxyethyl jasmonate (HEJA) was for the first time employed to induce the ginsenoside biosynthesis and to manipulate the product heterogeneity in plant cell cultures. The dose response and timing of HEJA elicitation were investigated in cell suspension cultures of Panax notoginseng. The opti...

journal_title：Applied microbiology and biotechnology

authors： Wang W,Zhao ZJ,Xu Y,Qian X,Zhong JJ

更新日期：2006-04-01 00:00:00

abstract：:Porcine parvovirus (PPV) virus-like particles (VLPs) constitute a potential vaccine for prevention of parvovirus-induced reproductive failure in gilts. Here we report the development of a large scale (25 l) production process for PPV-VLPs with baculovirus-infected insect cells. A low multiplicity of infection (MOI) st...

journal_title：Applied microbiology and biotechnology

authors： Maranga L,Rueda P,Antonis AF,Vela C,Langeveld JP,Casal JI,Carrondo MJ

更新日期：2002-06-01 00:00:00

abstract：:Functional genomics of filamentous fungi has gradually uncovered gene information for constructing 'cell factories' and controlling pathogens. Available gene manipulation methods of filamentous fungi include random integration methods, gene targeting technology, gene editing with artificial nucleases and RNA technolog...

journal_title：Applied microbiology and biotechnology

authors： Wang S,Chen H,Tang X,Zhang H,Chen W,Chen YQ

更新日期：2017-11-01 00:00:00

abstract：:Glucose 6-phosphate dehydrogenase (G6PDH) is one of the most important dehydrogenases responsible for generating reduced NADPH for anabolism and is also the rate-limiting enzyme in the Entner-Doudoroff pathway. For in vitro biocatalysis, G6PDH must possess both high activity and good thermostability due to requirement...

journal_title：Applied microbiology and biotechnology

authors： Huang R,Chen H,Zhou W,Ma C,Zhang YP

更新日期：2018-04-01 00:00:00

abstract：:In an effort to produce ricinoleic acid (RA), an important natural raw material with great values as a petrochemical replacement, in Schizosaccharomyces pombe, we introduced Claviceps purpurea oleate Δ12-hydroxylase gene (CpFAH12) to S. pombe, putting it under the control of an inducible nmt1 promoter. However, RA was...

journal_title：Applied microbiology and biotechnology

authors： Yazawa H,Holic R,Kumagai H,Uemura H

更新日期：2013-09-01 00:00:00

abstract：:Methane and nitrous oxide are both potent greenhouse gasses, with global warming potentials approximately 25 and 298 times that of carbon dioxide. A matrix of soil microcosms was constructed with landfill cover soils collected from the King Highway Landfill in Kalamazoo, Michigan and exposed to geochemical parameters ...

journal_title：Applied microbiology and biotechnology

authors： Lee SW,Im J,Dispirito AA,Bodrossy L,Barcelona MJ,Semrau JD

更新日期：2009-11-01 00:00:00