A simple method for isolation and construction of markerless cyanobacterial mutants defective in acyl-acyl carrier protein synthetase.

abstract：:Epitope vaccine based on the enzyme urease of Helicobacter pylori is a promising option for prophylactic and therapeutic vaccination against H. pylori infection. In our previous study, the epitope vaccine CTB-UA, which was composed of the mucosal adjuvant cholera toxin B subunit (CTB) and an epitope (UreA₁₈₃₋₂₀₃) from...

journal_title：Applied microbiology and biotechnology

authors： Guo L,Liu K,Xu G,Li X,Tu J,Tang F,Xing Y,Xi T

更新日期：2012-09-01 00:00:00

abstract：:Red koji or red mold rice is prepared by growing a genus Monascus on steamed rice. For centuries, it has been used in Asia for the production of fermented foods including red rice wine and fermented tofu. Although red koji is an important source of various hydrolytic enzymes critical for food fermentation, information...

journal_title：Applied microbiology and biotechnology

authors： Yasuda M,Tachibana S,Kuba-Miyara M

更新日期：2012-10-01 00:00:00

abstract：:The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a novel β-coronavirus, is the main pathogenic agent of the rapidly spreading pneumonia called coronavirus disease 2019 (COVID-19). SARS-CoV-2 infects much more people, especially the elder population, around the world than other coronavirus, such as SAR...

journal_title：Applied microbiology and biotechnology

authors： Chen X,Liao B,Cheng L,Peng X,Xu X,Li Y,Hu T,Li J,Zhou X,Ren B

更新日期：2020-09-01 00:00:00

abstract：:Lyme borreliosis is a bacterial infection that can be spread to humans by infected ticks and may severely affect many organs and tissues. Nearly four decades have elapsed since the discovery of the disease agent called Borrelia burgdorferi. Although there is a plethora of knowledge on the infectious agent and thousand...

journal_title：Applied microbiology and biotechnology

authors： Strnad M,Grubhoffer L,Rego ROM

更新日期：2020-03-01 00:00:00

abstract：:Using a reverse-transcription polymerase chain reaction (RT-PCR) technique, cytochrome P450 genes were cloned from the lignin-degrading basidiomycete, Coriolus versicolor. One possible P450 gene was identified, which consisted of 1,672 nucleotides and a poly(A) tail and encoded a deduced protein containing 449 amino a...

journal_title：Applied microbiology and biotechnology

authors： Ichinose H,Wariishi H,Tanaka H

更新日期：2002-01-01 00:00:00

abstract：:Soil microbial autotrophs play a significant role in CO2 fixation in terrestrial ecosystem, particularly in vegetation-constrained ecosystems with environmental stresses, such as the Tibetan Plateau characterized by low temperature and high UV. However, soil microbial autotrophic communities and their driving factors ...

journal_title：Applied microbiology and biotechnology

authors： Guo G,Kong W,Liu J,Zhao J,Du H,Zhang X,Xia P

更新日期：2015-10-01 00:00:00

abstract：:In oyster ponds, the marine diatom Haslea ostrearia synthesises and excretes a hydrosoluble pigment of commercial interest called marennin. During the benthic stage, when algal cells are naturally immobilised in their own polysaccharides, marennin production is higher. To optimise this production, axenic cultures of H...

journal_title：Applied microbiology and biotechnology

authors： Lebeau T,Gaudin P,Moan R,Robert JM

更新日期：2002-07-01 00:00:00

abstract：:Short interfering RNA (siRNA) is one of the members of the family of RNA interference (RNAi). Coupled with the RNA-induced silencing complex (RISC), siRNA is able to trigger the cleavage of target RNAs which serve as a defensive system against pathogens. Meanwhile, siRNA in gene silencing opens a new avenue for the tr...

journal_title：Applied microbiology and biotechnology

authors： Tam C,Wong JH,Cheung RCF,Zuo T,Ng TB

更新日期：2017-10-01 00:00:00

abstract：:Knowledge of tolerance of bacteria to toxic stress is important, especially for processes targeted at high final titers of product. Information on environmental limits and stress responses may help during selection of strains or design and control of processes. The influence of the main product and its co-products on ...

journal_title：Applied microbiology and biotechnology

authors： Szymanowska-Powałowska D,Kubiak P

更新日期：2015-04-01 00:00:00

abstract：:His-Asn-His (HNH) proteins are a very common family of small nucleic acid-binding proteins that are generally associated with endonuclease activity and are found in all kingdoms of life. Although HNH endonucleases from mesophiles have been widely investigated, the biochemical functions of HNH endonucleases from thermo...

journal_title：Applied microbiology and biotechnology

authors： Zhang L,Huang Y,Xu D,Yang L,Qian K,Chang G,Gong Y,Zhou X,Ma K

更新日期：2016-09-01 00:00:00

abstract：:Robustness of fermenting strains to lignocellulose derived inhibitors is critical for efficient biofuel and biochemical productions. In this study, the industrial fermenting strain Corynebacterium glutamicum S9114 was evolved for improved inhibitor tolerance using long-term adaptive evolution by continuously transferr...

journal_title：Applied microbiology and biotechnology

authors： Wang X,Khushk I,Xiao Y,Gao Q,Bao J

更新日期：2018-01-01 00:00:00

abstract：:The fungal secretome comprises various oxidative enzymes participating in the degradation of lignocellulosic biomass as a central step in carbon recycling. Among the secreted enzymes, aryl-alcohol oxidases (AAOs) are of interest for biotechnological applications including production of bio-based precursors for plastic...

journal_title：Applied microbiology and biotechnology

authors： Jankowski N,Koschorreck K,Urlacher VB

更新日期：2020-11-01 00:00:00

abstract：:Two recombinant Aeropyrum pernix glutamate dehydrogenase (GDH) enzymes with different length N-termini were cloned and expressed in Escherichia coli: sGDH begins with the amino acid sequence of the extracted native enzyme (M-Q-P-T-D-P-L-E-E), whereas lGDH begins with the sequence of the predicted ORF (M-E-V-L-A-L-Q-P-...

journal_title：Applied microbiology and biotechnology

authors： Helianti I,Morita Y,Murakami Y,Yokoyama K,Tamiya E

更新日期：2002-08-01 00:00:00

abstract：:A medium-scale (0.77 l) air-cathode, brush-anode microbial fuel cell (MFC) operated in fed-batch mode using xylose (20 mM) generated a maximum power density of 13 +/- 1 W/m(3) (673 +/- 43 mW/m(2)). Xylose was rapidly removed (83.5%) within 8 h of a 60-h cycle, with 42.1% of electrons in intermediates (8.5 +/- 0.2 mM a...

journal_title：Applied microbiology and biotechnology

authors： Huang L,Logan BE

更新日期：2008-09-01 00:00:00

abstract：:Prenyltransferases of the dimethylallyltryptophan synthase (DMATS) superfamily are involved in the biosynthesis of secondary metabolites and show broad substrate specificity towards their aromatic substrates with a high regioselectivity for the prenylation reactions. Most members of this superfamily accepted as prenyl...

journal_title：Applied microbiology and biotechnology

authors： Pockrandt D,Sack C,Kosiol T,Li SM

更新日期：2014-06-01 00:00:00

abstract：:The essential general features required for lipid membranes of extremophilic archaea to fulfill biological functions are that they are in the liquid crystalline phase and have extremely low permeability of solutes that is much less temperature sensitive due to a lack of lipid-phase transition and highly branched isopr...

journal_title：Applied microbiology and biotechnology

authors： Ulrih NP,Gmajner D,Raspor P

更新日期：2009-08-01 00:00:00

abstract：:Forty-eight single-use stirred tank bioreactors on a 10-mL scale operated in a magnetically inductive driven bioreaction block and automated with a liquid handler were applied for discrimination of different riboflavin producing Bacillus subtilis strains based on their performances in the parallel fed-batch processes....

journal_title：Applied microbiology and biotechnology

authors： Vester A,Hans M,Hohmann HP,Weuster-Botz D

更新日期：2009-08-01 00:00:00

abstract：:Bovine mastitis results in billion dollar losses annually in the USA alone. Streptococci are among the most relevant causative agents of this disease. Conventional antibiotic therapy is often unsuccessful and contributes to development of antibiotic resistance. Bacteriophage endolysins represent a new class of antimic...

journal_title：Applied microbiology and biotechnology

authors： Schmelcher M,Powell AM,Camp MJ,Pohl CS,Donovan DM

更新日期：2015-10-01 00:00:00

abstract：:The wide application of water-soluble linear polyacrylamides (PAMs) can cause serious environmental pollution. Biological treatment of PAMs receives very limited efficiency due to their recalcitrance to the microbial degradation. Here, we show the bioelectrochemical system (BES) can be used as an effective strategy to...

journal_title：Applied microbiology and biotechnology

authors： Cui YZ,Zhang J,Sun M,Zhai LF

更新日期：2015-01-01 00:00:00

abstract：:Respiration measurement in shake flasks is introduced as a new method to characterize the metabolic activity of microorganisms during and after stress exposure. The major advantage of the new method is the possibility to determine the metabolic activity independent of manual sampling without the necessity to change th...

journal_title：Applied microbiology and biotechnology

authors： Seletzky JM,Noack U,Fricke J,Hahn S,Büchs J

更新日期：2006-10-01 00:00:00

abstract：:A novel and efficient screening method for pyrroloquinoline quinone (PQQ) high-yielding methylotrophic strains was developed by using glucose dehydrogenase apoenzyme (GDHA) which depended on PQQ as the cofactor. Using this high-throughput method, PQQ high-yielding strains were rapidly screened out from thousands of me...

journal_title：Applied microbiology and biotechnology

authors： Si Z,Zhu J,Wang W,Huang L,Wei P,Cai J,Xu Z

更新日期：2016-12-01 00:00:00

abstract：:Two-dimensional gel electrophoresis was conducted to investigate the effect of H2O2 on whole protein expression in Acinetobacter oleivorans DR1. Functional classification of 13 upregulated proteins using MALDI-TOF mass spectrometry showed relationships with oxidative stress, energy production and conversion, nucleotid...

journal_title：Applied microbiology and biotechnology

authors： Kim J,Cho Y,Jang IA,Park W

更新日期：2015-12-01 00:00:00

abstract：:Previous studies have shown that a 20-kDa protein enhances production of the insecticidal CytA and CryIVA proteins of Bacillus thuringiensis in Escherichia coli as well as CytA production and crystal formation in B. thuringiensis. To determine whether the 20-kDa protein could enhance CryIVD production, an expression v...

journal_title：Applied microbiology and biotechnology

authors： Wu D,Federici BA

更新日期：1995-01-01 00:00:00

abstract：:Xanthomonas campestris pv. campestris, the causal agent of black-rot disease of cruciferous plants, and an important industrial microbe, was able to express the Escherichia coli beta-glucuronidase reporter gene (uidA) when fused to the E. coli lactose operon promoter on a wide-host-range plasmid vector. The gene fusio...

journal_title：Applied microbiology and biotechnology

authors： Soby SD,Daniels MJ

更新日期：1996-12-01 00:00:00

abstract：:Due to the emerging environmental issues related to heavy metals, concern about the soil quality of farming lands near manufacturing district is increasing. Investigating the function of soil microorganisms exposed to long-term heavy metal contamination is meaningful and important for agricultural soil utilization. Th...

journal_title：Applied microbiology and biotechnology

authors： Deng L,Zeng G,Fan C,Lu L,Chen X,Chen M,Wu H,He X,He Y

更新日期：2015-10-01 00:00:00

abstract：:We used experimental evolution in order to identify genes involved in the adaptation of Saccharomyces cerevisiae to the early stages of alcoholic fermentation. Evolution experiments were run for about 200 generations, in continuous culture conditions emulating the initial stages of wine fermentation. We performed whol...

journal_title：Applied microbiology and biotechnology

authors： Mangado A,Tronchoni J,Morales P,Novo M,Quirós M,Gonzalez R

更新日期：2015-02-01 00:00:00

abstract：:Porcine parvovirus type 1 (PPV1) is a major causative agent of embryonic and fetal death in swine. The PPV1 VP2 protein is closely associated with viral immunogenicity for eliciting neutralizing antibodies, but its antigenic structures have been largely unknown. We generated three monoclonal antibodies (MAbs) against ...

journal_title：Applied microbiology and biotechnology

authors： Sun J,Huang L,Wei Y,Wang Y,Chen D,Du W,Wu H,Feng L,Liu C

更新日期：2015-11-01 00:00:00

abstract：:Probiotics and bovine colostrums had been proven to be beneficial for human health. Lactobacillus rhamnosus ZDY114 and anti-Helicobacter pylori bovine colostrums were used for the preparation of microecological additives, and their synergistic antidigestion effect in the simulated gastrointestinal tract (in vitro) was...

journal_title：Applied microbiology and biotechnology

authors： Wei H,Xu Y,Xiong Y,Xu F,Liu G

更新日期：2007-06-01 00:00:00

abstract：:In microorganisms, the enzyme acetate kinase (AK) catalyses the formation of ATP from ADP by de-phosphorylation of acetyl phosphate into acetic acid. A mutant strain of Clostridium acetobutylicum lacking acetate kinase activity is expected to have reduced acetate and acetone production compared to the wild type. In th...

journal_title：Applied microbiology and biotechnology

authors： Kuit W,Minton NP,López-Contreras AM,Eggink G

更新日期：2012-05-01 00:00:00

abstract：:Transformants of the Trichoderma reeseistrains QM9414 and Rut-C30 were constructed in which the genes for the two major hydrophobin proteins, hydrophobins I (HFBI) and II (HFBII), were deleted or amplified by molecular biological techniques. Growth parameters and foam production of the transformant strains were compar...

journal_title：Applied microbiology and biotechnology

authors： Bailey MJ,Askolin S,Hörhammer N,Tenkanen M,Linder M,Penttilä M,Nakari-Setälä T

更新日期：2002-05-01 00:00:00