High-level expression of aryl-alcohol oxidase 2 from Pleurotus eryngii in Pichia pastoris for production of fragrances and bioactive precursors.

abstract：:(S)-Hydroxynitrile lyase from Manihot esculenta (MeHNL) was shown for the first time to be able to catalyze the enantioselective transcyanation of acetyltrimethylsilane (ATMS) with acetone cyanohydrin to form (S)-2-trimethylsilyl-2-hydroxyl-propionitrile in an aqueous/organic biphasic system. To better understand the ...

journal_title：Applied microbiology and biotechnology

authors： Xu R,Zong MH,Liu YY,He J,Zhang YY,Lou WY

更新日期：2004-11-01 00:00:00

abstract：:In this work, phytosterol-biotransforming strains were selected from Mycobacterium sp., using a high concentration of beta-sitosterol. The selection was made by culturing the strains in a medium enriched with 14 g beta-sitosterol/l as the unique source of carbon. During 2 months, the bacterial cultures were transferre...

journal_title：Applied microbiology and biotechnology

authors： Vidal M,Becerra J,Mondaca MA,Silva M

更新日期：2001-10-01 00:00:00

abstract：:Conventional acetone-butanol-ethanol (ABE) fermentation coupled with gas stripping is conducted under strict anaerobic conditions. In this work, a fed-batch ABE fermentation integrated with gas stripping (FAFIGS) system using a non-strict anaerobic butanol-producing symbiotic system, TSH06, was investigated for the ef...

journal_title：Applied microbiology and biotechnology

authors： Lin Z,Liu H,Yan X,Zhou Y,Cheng K,Zhang J

更新日期：2017-11-01 00:00:00

abstract：:A transcriptional activator for actinorhodin biosynthesis, AtrA, was previously characterized in Streptomyces coelicolor A3(2), and an orthologue of atrA, named aveI, is identified in the Streptomyces avermitilis NRRL8165 genome (Uguru et al., Mol Microbiol, 58:131-150, 2005). In this study, genetic and functional cha...

journal_title：Applied microbiology and biotechnology

authors： Chen L,Lu Y,Chen J,Zhang W,Shu D,Qin Z,Yang S,Jiang W

更新日期：2008-08-01 00:00:00

abstract：:Developing the production of ectomycorrhizal (ECM) mushrooms in forest has become a challenge. Only a few ECM species are currently cultivable. Controlled mycorrhization practices offer promising advance to produce currently uncultivable ECM mushrooms. The persistence of the production of edible species, either cultiv...

journal_title：Applied microbiology and biotechnology

authors： Savoie JM,Largeteau ML

更新日期：2011-02-01 00:00:00

abstract：:Agriculture surplus were used as substrates to synthesize gamma-aminobutyric acid (GABA) by Lactobacillus plantarum DSM19463 for the manufacture of a functional beverage or as a novel application for dermatological purposes. Dilution of the grape must to 1 or 4% (w/v) of total carbohydrates favored higher cell yield a...

journal_title：Applied microbiology and biotechnology

authors： Di Cagno R,Mazzacane F,Rizzello CG,De Angelis M,Giuliani G,Meloni M,De Servi B,Gobbetti M

更新日期：2010-03-01 00:00:00

abstract：:Linkages among bioreactor operation and performance and microbial community structure were investigated for a fixed-bed biofilm system designed to remove perchlorate from drinking water. Perchlorate removal was monitored to evaluate reactor performance during and after the frequency and intensity of the backwash proce...

journal_title：Applied microbiology and biotechnology

authors： Li X,Yuen W,Morgenroth E,Raskin L

更新日期：2012-11-01 00:00:00

abstract：:We previously showed that opdA from Sphingomonas sp. PWE1 encodes a putative flavin monooxygenase capable of transforming octylphenol (OP) via type II ipso substitution. Here, we demonstrate that an opdA homolog is responsible for OP and related alkyl/alkoxyphenol degradation in the nonylphenol degrader Sphingomonas s...

journal_title：Applied microbiology and biotechnology

authors： Porter AW,Campbell BR,Kolvenbach BA,Corvini PF,Benndorf D,Rivera-Cancel G,Hay AG

更新日期：2012-04-01 00:00:00

abstract：:Bacterial glucans have aroused increasing interest in commercial applications in the food and pharmaceutical sectors. A number of bacterial glucans have been reported over recent decades, and their structure, production, and functional properties have been extensively studied. In this paper, we review recent researche...

journal_title：Applied microbiology and biotechnology

authors： Xu L,Zhang J

更新日期：2016-11-01 00:00:00

abstract：:To reveal the succession procedure of ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) community structure in sequencing biofilm batch reactor (SBBR), the molecular biological techniques of denaturing gradient gel electrophoresis (DGGE), cloning, and real-time PCR were applied. DGGE showed that th...

journal_title：Applied microbiology and biotechnology

authors： Wang J,Zhang C,Rong H

更新日期：2014-05-01 00:00:00

abstract：:Escherichia coli cells expressing mink (Mustela vison) growth hormone were grown in a batch fermentation process. The expression level was estimated to be 27% of the total cellular protein after 3 h of induction with 1 mM isopropyl beta-D-thiogalactoside (IPTG). If the expression of mink growth hormone (mGH) was induc...

journal_title：Applied microbiology and biotechnology

authors： Sereikaite J,Statkute A,Morkunas M,Radzevicius K,Borromeo V,Secchi C,Bumelis VA

更新日期：2007-02-01 00:00:00

abstract：:Streptococcus mutans is the main cause of dental caries. We evaluated the therapeutic effect of variable regions of a llama heavy chain antibody fragments directed against S. mutans named S36-VHH (S for Streptococcus) alone or fused with glucose oxidase (GOx) from Aspergillus niger. Western blot analysis and ELISA rev...

journal_title：Applied microbiology and biotechnology

authors： Krüger C,Hultberg A,Marcotte H,Hermans P,Bezemer S,Frenken LG,Hammarström L

更新日期：2006-10-01 00:00:00

abstract：:Cytidine diphosphate choline (CDP-choline) has been applied for treating acute craniocerebral injury and allowing recovery of consciousness after brain surgery. In this study, an acetate kinase (ACK)/acetyl phosphate system was used to supply ATP and combined with Escherichia coli-overexpressed CMP kinase (CMK), NDP k...

journal_title：Applied microbiology and biotechnology

authors： Liu Y,Wang J,Xu C,Chen Y,Yang J,Liu D,Niu H,Jiang Y,Yang S,Ying H

更新日期：2017-02-01 00:00:00

abstract：:The basidiomycete Cystoderma carcharias transformed citronellol into 3,7-dimethyl-1,6,7-octanetriol as the main product. 3,7-Dimethyl-6,7-epoxy-1-octanol was identified as important intermediary product of the biotransformation, and the allylic diols 2,6-dimethyl-2-octene-1,8-diol, 3,7-dimethyl-5-octene-1,7-diol and 3...

journal_title：Applied microbiology and biotechnology

authors： Onken J,Berger RG

更新日期：1999-02-01 00:00:00

abstract：:For ethical, technical, regulatory, and cost reasons, in vitro methods are increasingly used as an alternative to in vivo experimentations. The aim of the present study was to validate, according to in vivo data in living animals, a new in vitro model of the piglet colon, the PigutIVM, under both control conditions an...

journal_title：Applied microbiology and biotechnology

authors： Fleury MA,Le Goff O,Denis S,Chaucheyras-Durand F,Jouy E,Kempf I,Alric M,Blanquet-Diot S

更新日期：2017-03-01 00:00:00

abstract：:Laccases are blue multicopper oxidases with potential applications in environmental and industrial biotechnology. In this study, a new bacterial laccase gene of 1.32 kb was obtained from a marine microbial metagenome of the South China Sea by using a sequence screening strategy. The protein (named as Lac15) of 439 ami...

journal_title：Applied microbiology and biotechnology

authors： Fang Z,Li T,Wang Q,Zhang X,Peng H,Fang W,Hong Y,Ge H,Xiao Y

更新日期：2011-02-01 00:00:00

abstract：:The Gram-positive bacterium, Bacillus subtilis and related species are widely used industrially as hosts for producing enzymes. These species possess a high potential to produce secreted proteins into the culture medium. Nevertheless, the secretion of heterologous proteins by these species is frequently inefficient. I...

journal_title：Applied microbiology and biotechnology

authors： Kakeshita H,Kageyama Y,Ara K,Ozaki K,Nakamura K

更新日期：2011-03-01 00:00:00

abstract：:Biogranulation is a promising biotechnology developed for wastewater treatment. Biogranules exhibit a matrix microbial structure, and intensive research has shown that extracellular polymeric substances (EPS) are a major component of the biogranule matrix material in both anaerobic and aerobic granules. This paper aim...

journal_title：Applied microbiology and biotechnology

authors： Liu YQ,Liu Y,Tay JH

更新日期：2004-08-01 00:00:00

abstract：:Starting from mature vegetable compost, four bacterial strains were selected using a lignin-rich medium. 16S ribosomal RNA identification of the isolates showed high score similarity with Pseudomonas spp. for three out of four isolates. Further characterization of growth on mixtures of six selected lignin model compou...

journal_title：Applied microbiology and biotechnology

authors： Ravi K,García-Hidalgo J,Gorwa-Grauslund MF,Lidén G

更新日期：2017-06-01 00:00:00

abstract：:In this study, we used the non-carotenogenic yeast Pichia pastoris X33 as a receptor for β-carotene-encoding genes, in order to obtain new recombinant strains capable of producing different carotenoidic compounds. We designed and constructed two plasmids, pGAPZA-EBI* and pGAPZA-EBI*L*, containing the genes encoding ly...

journal_title：Applied microbiology and biotechnology

authors： Araya-Garay JM,Feijoo-Siota L,Rosa-dos-Santos F,Veiga-Crespo P,Villa TG

更新日期：2012-03-01 00:00:00

abstract：:Thirty-four strains of lactic acid bacteria (seven Bifidobacterium, 11 Lactobacillus, six Lactococcus, and 10 Streptococcus thermophilus) were assayed in vitro for antioxidant activity against ascorbic and linolenic acid oxidation (TAA(AA) and TAA(LA)), trolox-equivalent antioxidant capacity (TEAC), intracellular glut...

journal_title：Applied microbiology and biotechnology

authors： Amaretti A,di Nunzio M,Pompei A,Raimondi S,Rossi M,Bordoni A

更新日期：2013-01-01 00:00:00

abstract：:Understanding the effect of conserved domains reconstruction of alginate lyases on action mode is essential for their application and in-depth study. We report the expression and action mode of recombinant alginate lyase (AlyM) and its conserved domain reconstruction forms (AlyMΔCBM, cAlyM, and AlyMΔ58C). The enzymati...

journal_title：Applied microbiology and biotechnology

authors： Yang M,Li N,Yang S,Yu Y,Han Z,Li L,Mou H

更新日期：2019-01-01 00:00:00

abstract：:The use of surfactin and mycosubtilin as an eco-friendly alternative to control lettuce downy mildew caused by the obligate pathogen Bremia lactucae was investigated. Preliminary ecotoxicity evaluations obtained from three different tests revealed the rather low toxicity of these lipopeptides separately or in combinat...

journal_title：Applied microbiology and biotechnology

authors： Deravel J,Lemière S,Coutte F,Krier F,Van Hese N,Béchet M,Sourdeau N,Höfte M,Leprêtre A,Jacques P

更新日期：2014-01-01 00:00:00

abstract：:The saprotrophic fungus Penicillium griseofulvum was chosen as model organism to study responses to a mixture of hexachlorocyclohexane (HCH) isomers (α-HCH, β-HCH, γ-HCH, δ-HCH) and potentially toxic metals (vanadium, lead) in solid and liquid media. The P. griseofulvum FBL 500 strain was isolated from polluted soil c...

journal_title：Applied microbiology and biotechnology

authors： Ceci A,Pinzari F,Riccardi C,Maggi O,Pierro L,Petrangeli Papini M,Gadd GM,Persiani AM

更新日期：2018-01-01 00:00:00

abstract：:Synthetic logs were vacuum-soaked or regular-soaked to determine the effects of soaking on yield and mushroom size, log weight variability and water distribution within the log. Yields (g/log) from substrates vacuum-soaked were higher by 26.7%, 18.6% and 35.8% (mean = 27.2%) for crops I, II and III, respectively, when...

journal_title：Applied microbiology and biotechnology

authors： Royse DJ,Rhodes TW,Sanchez JE

更新日期：2002-01-01 00:00:00

abstract：:The redox-sensing transcriptional repressor Rex (Rex) displayed diverse functions in different microbial species. Nowadays, only part function of rex has been verified in vitro and alcohol dehydrogenase gene (adhE) as the target of Rex has been widely reported. In this study, rex was knocked out in Thermoanaerobacteri...

journal_title：Applied microbiology and biotechnology

authors： Qu C,Chen L,Li Y,Fu H,Wang J

更新日期：2020-06-01 00:00:00

abstract：:When sucrose was used as the carbon source, the Basidiomycete Coprinopsis cinerea showed poor growth and low laccase activity in pure culture, but greatly enhanced the level of laccase activity (>1800 U/L) during coculture with the Mucoromycete Gongronella sp. w5. As a result, the mechanism of laccase overproduction i...

journal_title：Applied microbiology and biotechnology

authors： Hu J,Zhang Y,Xu Y,Sun Q,Liu J,Fang W,Xiao Y,Kües U,Fang Z

更新日期：2019-01-01 00:00:00

abstract：:Potato pulp is a poorly utilized, high-volume co-processing product resulting from industrial potato starch manufacturing. Potato pulp mainly consists of the tuber plant cell wall material and is particularly rich in pectin, notably galactan branched rhamnogalacturonan I type pectin which has previously been shown to ...

journal_title：Applied microbiology and biotechnology

authors： Thomassen LV,Vigsnæs LK,Licht TR,Mikkelsen JD,Meyer AS

更新日期：2011-05-01 00:00:00

abstract：:Biotechnological production of vanillin is gaining momentum as the natural synthesis of vanillin that is very expensive. Ferulic acid (FA), a costly compound, is used as the substrate to produce vanillin biotechnologically and the making process is still expensive. Therefore, this study investigated the practical use ...

journal_title：Applied microbiology and biotechnology

authors： Chakraborty D,Selvam A,Kaur B,Wong JWC,Karthikeyan OP

更新日期：2017-07-01 00:00:00

abstract：:After crude protein of the marine yeast strains maintained in this laboratory was estimated by the method of Kjehldahl, we found that the G7a strain which was identified to be a strain of Cryptococcus aureus according to the routine identification and molecular methods contained high level of protein and could grow on...

journal_title：Applied microbiology and biotechnology

authors： Gao L,Chi Z,Sheng J,Ni X,Wang L

更新日期：2007-12-01 00:00:00