The redox-sensing transcriptional repressor Rex is important for regulating the products distribution in Thermoanaerobacterium aotearoense SCUT27.

abstract：:A set of filamentous fungi (42 strains) was screened for alpha-N-acetylgalactosaminidase activity, and a series of inducers and different cultivation conditions were tested. Enzyme production by the best producer Aspergillus niger CCIM K2 was optimized and scaled up. alpha-N-Acetylgalactosaminidase was purified to app...

journal_title：Applied microbiology and biotechnology

authors： Weignerová L,Filipi T,Manglová D,Kren V

更新日期：2008-07-01 00:00:00

abstract：:Sulfate (SO42-) is an often-utilized and well-understood inorganic sulfur source in microorganism culture. Recently, another inorganic sulfur source, thiosulfate (S2O32-), was proposed to be more advantageous in microbial growth and biotechnological applications. Although its assimilation pathway is known to depend on...

journal_title：Applied microbiology and biotechnology

authors： Kawano Y,Onishi F,Shiroyama M,Miura M,Tanaka N,Oshiro S,Nonaka G,Nakanishi T,Ohtsu I

更新日期：2017-09-01 00:00:00

abstract：:Several ansamycins have been reported to inhibit bacterial biofilm formation and accelerate the eradication of developed biofilms, but little is known about the effect of hygrocin C, an ansamycin, on bacterial biofilm formation. Here, hygrocin C was isolated from the marine-derived Streptomyces sp. SCSGAA 0027 and rep...

journal_title：Applied microbiology and biotechnology

authors： Wang J,Nong XH,Amin M,Qi SH

更新日期：2018-02-01 00:00:00

abstract：:This study focused on the use the thermophilic anaerobic granulae in the start-up of 70 degrees C acetate-fed upflow anaerobic sludge-blanket (UASB) reactors and the kinetics of granulae grown at 70 degrees C. In the UASB reactors, chemical oxygen demand removal commenced within 48 h of the start-up. The maximum reduc...

journal_title：Applied microbiology and biotechnology

authors： Lepistö R,Rintala JA

更新日期：1995-11-01 00:00:00

abstract：:The Gram-positive bacterium, Bacillus subtilis and related species are widely used industrially as hosts for producing enzymes. These species possess a high potential to produce secreted proteins into the culture medium. Nevertheless, the secretion of heterologous proteins by these species is frequently inefficient. I...

journal_title：Applied microbiology and biotechnology

authors： Kakeshita H,Kageyama Y,Ara K,Ozaki K,Nakamura K

更新日期：2011-03-01 00:00:00

abstract：:Epoxide hydrolases (EHs) of fungal origin have the ability to catalyze the enantioselective hydrolysis of epoxides to their corresponding diols. However, wild type fungal EHs are limited in substrate range and enantioselectivity. Additionally, the production of fungal epoxide hydrolase (EH) by wild-type strains is typ...

journal_title：Applied microbiology and biotechnology

authors： Maharajh D,Roth R,Lalloo R,Simpson C,Mitra R,Görgens J,Ramchuran S

更新日期：2008-05-01 00:00:00

abstract：:Biocatalyzed synthesis of nucleoside analogues was carried out using two thermostable nucleoside phosphorylases from the hyperthermophilic aerobic crenarchaeon Aeropyrum pernix K1. The synthesis of the 2,6-diaminopurine nucleoside and 5-methyluridine was used as a reaction model to test the process. Both the purine nu...

journal_title：Applied microbiology and biotechnology

authors： Zhu S,Song D,Gong C,Tang P,Li X,Wang J,Zheng G

更新日期：2013-08-01 00:00:00

abstract：:The Ser-170 residue of a trypsin-like enzyme from Streptomyces fradiae (SFT), which is considered to be the active-site serine, was replaced with alanine by site-directed mutagenesis to improve the affinity chromatography step for a Kazal-type trypsin inhibitor pancreatic secretory trypsin inhibitor (PSTI). The result...

journal_title：Applied microbiology and biotechnology

authors： Katoh T,Kikuchi N,Nagata K,Yoshida N

更新日期：1996-08-01 00:00:00

abstract：:Confocal Raman microspectral imaging (CRMI) is an advanced cell-imaging method that maps endogenous molecular compositions with their unique spectral fingerprint indicators. The aim of this work was to provide a visualized understanding of subcellular features of live osteosarcoma cells using a 532-nm laser excitation...

journal_title：Applied microbiology and biotechnology

authors： Li J,Qin J,Zhang X,Wang R,Liang Z,He Q,Wang Z,Wang K,Wang S

更新日期：2019-08-01 00:00:00

abstract：:Ammonium can be removed as dinitrogen gas by cooperating aerobic and anaerobic ammonium-oxidizing bacteria (AerAOB and AnAOB). The goal of this study was to verify putative mutual benefits for aggregated AerAOB and AnAOB in a stagnant freshwater environment. In an ammonium fed water column, the biological oxygen consu...

journal_title：Applied microbiology and biotechnology

authors： Vlaeminck SE,Dierick K,Boon N,Verstraete W

更新日期：2007-07-01 00:00:00

abstract：:κ-Carrageenases exhibit apparent distinctions in gene sequence, molecular weight, enzyme properties, and posttranslational processes. In this study, a new κ-carrageenase gene named cgkZ was cloned from the marine bacterium Zobellia sp. ZM-2. The gene comprised an open reading frame of 1,638 bp and encoded 545 amino ac...

journal_title：Applied microbiology and biotechnology

authors： Liu Z,Li G,Mo Z,Mou H

更新日期：2013-12-01 00:00:00

abstract：:Two-dimensional gel electrophoresis was conducted to investigate the effect of H2O2 on whole protein expression in Acinetobacter oleivorans DR1. Functional classification of 13 upregulated proteins using MALDI-TOF mass spectrometry showed relationships with oxidative stress, energy production and conversion, nucleotid...

journal_title：Applied microbiology and biotechnology

authors： Kim J,Cho Y,Jang IA,Park W

更新日期：2015-12-01 00:00:00

abstract：:LI-F type peptides are a family of cyclic lipodepsipeptide antibiotics isolated from Paenibacillus polymyxa and display potent activities against positive bacteria including methicillin-resistant S. aureus (MRSA). In this study, we investigated the mechanism of action of LI-F type peptide AMP-jsa9 against a MRSA (S. a...

journal_title：Applied microbiology and biotechnology

authors： Han J,Ma Z,Gao P,Lu Z,Liu H,Gao L,Lu W,Ju X,Lv F,Zhao H,Bie X

更新日期：2018-03-01 00:00:00

abstract：:Soy sauce yeast Zygosaccharomyces rouxii plays a central role in the production of flavor compounds in soy sauce, while the flor-forming strain spoils its quality by producing 2-methylpropanoic acid, 2-methylbutanoic acid, and 3-methylbutanoic acid, which have an unpleasant odor. To investigate the relationship betwee...

journal_title：Applied microbiology and biotechnology

authors： Watanabe J,Uehara K,Mogi Y

更新日期：2015-09-01 00:00:00

abstract：:Variable domains of heavy chains of camelid heavy-chain antibodies (VHHs) are known as nanobodies. Nanobodies are approximately 15 kDa in size with high affinity to their antigens. They can be easily manipulated and produced in microorganisms. In this study, an alpaca was immunized with purified green fluorescence pro...

journal_title：Applied microbiology and biotechnology

authors： Fang Z,Cao D,Qiu J

更新日期：2020-06-01 00:00:00

abstract：:A fluidized-bed reactor (FBR) was used to enrich an aerobic chlorophenol-degrading microbial culture. Long-term continuous-flow operation with low effluent concentrations selected oligotrophic microorganisms producing good-quality effluent for pentachlorophenol(PCP)-contaminated water. PCP biodegradation kinetics was ...

journal_title：Applied microbiology and biotechnology

authors： Melin ES,Ferguson JF,Puhakka JA

更新日期：1997-06-01 00:00:00

abstract：:Corynebacterium glutamicum CCTCC M201005 produces a novel polygalacturonic acid bioflocculant, REA-11, consisting of galacturonic acid as the main structural unit. A biosynthetic pathway of REA-11 in C. glutamicum CCTCC M201005 was proposed. Evidence for the biosynthetic pathway was provided by: (1) analyzing the resp...

journal_title：Applied microbiology and biotechnology

authors： Li Y,He N,Guan H,Du G,Chen J

更新日期：2003-12-01 00:00:00

abstract：:Oxygenases-based Escherichia coli whole-cell biocatalyst can be applied for catalysis of various commercially interesting reactions that are difficult to achieve with traditional chemical catalysts. However, substrates and products of interest are often toxic to E. coli, causing a disruption of cell membrane. Therefor...

journal_title：Applied microbiology and biotechnology

authors： Siriphongphaew A,Pisnupong P,Wongkongkatep J,Inprakhon P,Vangnai AS,Honda K,Ohtake H,Kato J,Ogawa J,Shimizu S,Urlacher VB,Schmid RD,Pongtharangkul T

更新日期：2012-07-01 00:00:00

abstract：:In this study, the kinetic behaviors between n-butyl acetate and composite bead were investigated. Both microbial growth rate and biochemical reaction rate would be inhibited with increasing average inlet concentration. The order of the inhibitive effect, which resulted from increased average inlet concentration for f...

journal_title：Applied microbiology and biotechnology

authors： Chan WC,Hwang ID

更新日期：2006-11-01 00:00:00

abstract：:Surfaces of massive chalcopyrite (CuFeS2) electrodes were modified by applying variable oxidation potential pulses under growth media in order to induce the formation of different secondary phases (e.g., copper-rich polysulfides, S n(2-); elemental sulfur, S(0); and covellite, CuS). The evolution of reactivity (oxidat...

journal_title：Applied microbiology and biotechnology

authors： Lara RH,García-Meza JV,González I,Cruz R

更新日期：2013-03-01 00:00:00

abstract：:Phenylpropanoids as abundant, lignin-derived compounds represent sustainable feedstocks for biotechnological production processes. We found that the biotechnologically important soil bacterium Corynebacterium glutamicum is able to grow on phenylpropanoids such as p-coumaric acid, ferulic acid, caffeic acid, and 3-(4-h...

journal_title：Applied microbiology and biotechnology

authors： Kallscheuer N,Vogt M,Kappelmann J,Krumbach K,Noack S,Bott M,Marienhagen J

更新日期：2016-02-01 00:00:00

abstract：:We report an immuno-magnetic separation system developed by the immobilization of pathogen-specific antibodies on the core-shell magnetic beads. The magnetic beads were grafted with glycidylmethacrylate (GMA) using surface-initiated atom transfer radical polymerization (SI-ATRP). For immuno-magnetic separation (IMS) o...

journal_title：Applied microbiology and biotechnology

authors： Ozalp VC,Bayramoglu G,Arica MY,Oktem HA

更新日期：2013-11-01 00:00:00

abstract：:The process for the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB/V)] by bacterial fermentation and its recovery was analysed. The effects of various factors such as P(3HB/V) content, P(3HB/V) productivity, P(3HB/V) yield and 3-hydroxyvalerate (3HV) fraction in P(3HB/V) on the production cost of P(...

journal_title：Applied microbiology and biotechnology

authors： Choi J,Lee SY

更新日期：2000-06-01 00:00:00

abstract：:A new medium supplement mixture, PPRF92, has been developed to enable the serial subculture of human diploid fibroblasts (MRC-5 cells) on microcarriers. Furthermore, the PPRF92 supplements enable cell growth at serum levels as low as 1%. Through an optimization programme, the PPRF92 supplements have evolved into a sim...

journal_title：Applied microbiology and biotechnology

authors： Forestell SP,Kalogerakis N,Behie LA

更新日期：1992-11-01 00:00:00

abstract：:Acidogenesis is an important pretreatment process for various industrial wastewater treatments. Granular sludge is an efficient form of a microbial community in anaerobic methanogenic reactors, such as upflow anaerobic sludge blanket (UASB), but it is hard to develop in the acidogenic process due to the short hydrauli...

journal_title：Applied microbiology and biotechnology

authors： Li Y,Zhang Y,Zhao Z,Sun S,Quan X,Zhao H

更新日期：2016-04-01 00:00:00

abstract：:Garlic oil can disrupt the quorum sensing (QS) pathways of the opportunistic pathogen Pseudomonas aeruginosa; however, the underlying mechanisms for this effect are unclear. Diallyl disulfide (DADS) is one of the most abundant sulfur-containing compounds in garlic oil. This study investigated the effects of DADS on th...

journal_title：Applied microbiology and biotechnology

authors： Li WR,Ma YK,Shi QS,Xie XB,Sun TL,Peng H,Huang XM

更新日期：2018-09-01 00:00:00

abstract：:The biofilm formation of Pseudomonas aeruginosa, an opportunistic human pathogen, is developed by cell-to-cell signaling, so-called quorum sensing (QS). To control the biofilm formation, we designed and synthesized new QS inhibitors of P. aeruginosa based on the structure of the previously known QS inhibitor, furanone...

journal_title：Applied microbiology and biotechnology

authors： Kim C,Kim J,Park HY,Park HJ,Lee JH,Kim CK,Yoon J

更新日期：2008-08-01 00:00:00

abstract：:In this work, the production of 1,3-propanediol from glucose and molasses was studied in a two-step process using two recombinant microorganisms. The first step of the process is the conversion of glucose or other sugar into glycerol by the metabolic engineered Saccharomyces cerevisiae strain HC42 adapted to high (>20...

journal_title：Applied microbiology and biotechnology

authors： Mendes FS,González-Pajuelo M,Cordier H,François JM,Vasconcelos I

更新日期：2011-11-01 00:00:00

abstract：:Aptamers are single-stranded nucleic acids or peptides identified from a randomized combinatorial library through specific interaction with the target of interest. Targets can be of any size, from small molecules to whole cells, attesting to the versatility of aptamers for binding a wide range of targets. Aptamers sho...

journal_title：Applied microbiology and biotechnology

authors： Gopinath SC,Lakshmipriya T,Chen Y,Arshad MK,Kerishnan JP,Ruslinda AR,Al-Douri Y,Voon CH,Hashim U

更新日期：2016-08-01 00:00:00

abstract：:Biodegradation of petroleum compounds in saline environments seems intricate and needs more attention. In this study, tetracosane was used to enrich alkane-degrading bacteria from oil-contaminated saline soils. Among the isolates, strain Qtet3, with the highest 16s rRNA gene sequence similarity to Alcanivorax dieselol...

journal_title：Applied microbiology and biotechnology

authors： Dastgheib SM,Amoozegar MA,Khajeh K,Ventosa A

更新日期：2011-04-01 00:00:00