Economic considerations in the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by bacterial fermentation.

abstract：:Over 9,000 flavonoid compounds have been found in various plants, comprising one of the largest families of natural products. Flavonoids are an essential factor in plant interactions with the environment, often serving as the first line of defense against UV irradiation and pathogen attacks. Flavonoids are also major ...

journal_title：Applied microbiology and biotechnology

authors： Wang Y,Chen S,Yu O

更新日期：2011-08-01 00:00:00

abstract：:Clostridium acetobutylicum is an important industrial microorganism for n-butanol bioproduction, and its transcription factor AbrB0310 regulates various important cellular processes. However, the roles of two abrB homologues, abrB1941 and abrB3647, have not been determined because they appear inactive during transcrip...

journal_title：Applied microbiology and biotechnology

authors： Xue Q,Yang Y,Chen J,Chen L,Yang S,Jiang W,Gu Y

更新日期：2016-11-01 00:00:00

abstract：:More than 70,000 different terpenoid structures are known so far; many of them offer highly interesting applications as pharmaceuticals, flavors and fragrances, or biofuels. Extraction of these compounds from their natural sources or chemical synthesis is-in many cases-technically challenging with low or moderate yiel...

journal_title：Applied microbiology and biotechnology

authors： Moser S,Pichler H

更新日期：2019-07-01 00:00:00

abstract：:We have recently reported that expression of an unidentified heme protein is enhanced in a nitrifying activated sludge community under low (0.1 mg O2/L) dissolved oxygen (DO) conditions. A preliminary assessment suggested it may be a type of hemoglobin (Hb) or a lesser-known component of the energy-transducing pathway...

journal_title：Applied microbiology and biotechnology

authors： Arnaldos M,Kunkel SA,Stark BC,Pagilla KR

更新日期：2014-04-01 00:00:00

abstract：:There is a growing demand for silver-based biocides, including both ionic silver forms and metallic nanosilver. The use of metallic nanosilver, typically chemically produced, faces challenges including particle agglomeration, high costs, and upscaling difficulties . Additionally, there exists a need for the developmen...

journal_title：Applied microbiology and biotechnology

authors： Sintubin L,De Windt W,Dick J,Mast J,van der Ha D,Verstraete W,Boon N

更新日期：2009-09-01 00:00:00

abstract：:Chitooligosaccharides have important application value in the fields of food and agriculture. Chitosanase can degrade chitosan to obtain chitooligosaccharides. The marine metagenome contains many genes related to the degradation of chitosan. However, it is difficult to mine valuable genes from large gene resources. Th...

journal_title：Applied microbiology and biotechnology

authors： Han Y,Guan F,Sun J,Wu N,Tian J

更新日期：2020-08-01 00:00:00

abstract：:2-Hydroxyalkanoates (2HAs) have become the new monomeric constituents of bacterial polyhydroxyalkanoates (PHAs). PHAs containing 2HA monomers, lactate (LA), glycolate (GL), and 2-hydroxybutyrate (2HB) can be synthesized by engineered microbes in which the broad substrate specificities of PHA synthase and propionyl-CoA...

journal_title：Applied microbiology and biotechnology

authors： Matsumoto K,Taguchi S

更新日期：2013-09-01 00:00:00

abstract：:Bacterial glucans have aroused increasing interest in commercial applications in the food and pharmaceutical sectors. A number of bacterial glucans have been reported over recent decades, and their structure, production, and functional properties have been extensively studied. In this paper, we review recent researche...

journal_title：Applied microbiology and biotechnology

authors： Xu L,Zhang J

更新日期：2016-11-01 00:00:00

abstract：:The synergistic effect between polyoxometalates (POMs), namely K(5)[SiW(11)V(V)O(40)]·11H(2)O and H(5)[PMo(10)V(V) (2)O(40)]·13H(2)O and laccase from ascomycete Myceliophthora thermophila has been employed for the first time in oxidative polymerization of catechol. Such a laccase-mediator system allowed the formation ...

journal_title：Applied microbiology and biotechnology

authors： Kim S,Silva C,Evtuguin DV,Gamelas JA,Cavaco-Paulo A

更新日期：2011-02-01 00:00:00

abstract：:Nine anaerobic promoters were cloned and constructed upstream of PHB synthesis genes phbCAB from Ralstonia eutropha for the micro- or anaerobic PHB production in recombinant Escherichia coli. Among the promoters, the one for alcohol dehydrogenase (PadhE) was found most effective. Recombinant E. coli JM 109 (pWCY09) ha...

journal_title：Applied microbiology and biotechnology

authors： Wei XX,Shi ZY,Yuan MQ,Chen GQ

更新日期：2009-03-01 00:00:00

abstract：:The phenomenon of the cell density effect is not readily explained by an obvious nutrient limitation, and a recent study has suggested that for recombinant Autographa californica multiple nucleopolyhedrovirus (rAcMNPV)-infected Sf9 cells, a drop in messenger RNA (mRNA) levels may be sufficient to explain the cell dens...

journal_title：Applied microbiology and biotechnology

authors： Huynh HT,Tran TT,Chan LC,Nielsen LK,Reid S

更新日期：2015-02-01 00:00:00

abstract：:Bacillus thuringiensis is well known as an effective bio-insecticidal bacterium. However, the roles of B. thuringiensis to control plant diseases are not paid great attention to. In recent years, many new functions in protecting plants from pathogen infection have been discovered. For example, acyl homoserine lactone ...

journal_title：Applied microbiology and biotechnology

authors： Zhou Y,Choi YL,Sun M,Yu Z

更新日期：2008-09-01 00:00:00

abstract：:A whole-cell bacterial biosensor for measuring aqueous concentrations of aromatic aldehydes was developed. It is based on the E. coli BL21DE3(RIL) expressing the green fluorescent protein under the control of an alcohol dehydrogenase inducible promoter belonging to the archaeon Sulfolobus solfataricus (Sso2536adh prom...

journal_title：Applied microbiology and biotechnology

authors： Fiorentino G,Ronca R,Bartolucci S

更新日期：2009-02-01 00:00:00

abstract：:The white button mushroom Agaricus bisporus is economically the most important commercially produced edible fungus. It is grown on carbon- and nitrogen-rich substrates, such as composted cereal straw and animal manure. The commercial mushroom production process is usually performed in buildings or tunnels under highly...

journal_title：Applied microbiology and biotechnology

authors： Kabel MA,Jurak E,Mäkelä MR,de Vries RP

更新日期：2017-06-01 00:00:00

abstract：:Ene-reductases originating from extremophiles are gaining importance in the field of biocatalysis due to higher-stability properties. The genome of the acidophilic iron-oxidizing bacterium "Ferrovum" sp. JA12 was found to harbor a thermophilic-like ene-reductase (FOYE-1). The foye-1 gene was ligated into a pET16bp exp...

journal_title：Applied microbiology and biotechnology

authors： Scholtissek A,Ullrich SR,Mühling M,Schlömann M,Paul CE,Tischler D

更新日期：2017-01-01 00:00:00

abstract：:Interleukin-2 (IL-2) is one of the most successful cytokines applied in tumor immunotherapy because of its ability to stimulate potent cellular immune response. The life-threatening toxicity of vascular leak syndrome (VLS) associated with the high-dose IL-2 treatment regimen has limited its use in tumor immunotherapy....

journal_title：Applied microbiology and biotechnology

authors： Ye L,Fan J,Shi X,Tao Q,Ye D,Xian Z,Zeng X,Li Y,Feng M,Ju D

更新日期：2014-05-01 00:00:00

abstract：:For the first time, Lactobacillus plantarum strains carrying heterologous genes encoding multifunctional glycoside hydrolases were constructed and used as additives for alfalfa silage. The chemical characteristics, nonstructural carbohydrate composition, and fermentation quality of alfalfa silage were examined. The su...

journal_title：Applied microbiology and biotechnology

authors： Guo J,Xie Y,Yu Z,Meng G,Wu Z

更新日期：2019-10-01 00:00:00

abstract：:The mevalonate pathway plays an important role in providing the cell with a number of essential precursors for the synthesis of biomass constituents. With respect to their chemical structure, the metabolites of this pathway can be divided into two groups: acyl esters [acetoacetyl CoA, acetyl CoA, hydroxymethylglutaryl...

journal_title：Applied microbiology and biotechnology

authors： Seker T,Møller K,Nielsen J

更新日期：2005-04-01 00:00:00

abstract：:The behaviour of dry baker's yeast ( Saccharomyces cerevisiae type II, Sigma) used as biocatalyst without preliminary growth for the synthesis of 2-heptanol from 2-heptanone in a biphasic system is presented. Cells undergo intracellular trehalose consumption with a stoichiometric ethanol production during the first 15...

journal_title：Applied microbiology and biotechnology

authors： Cappaert L,Larroche C

更新日期：2004-06-01 00:00:00

abstract：:In this work, a three-level Salmonella detection system based on a combination of seven SYBR®Green qPCR was developed. This detection system discriminates Salmonella at the genus, species and subspecies levels using a single 96-well plate. The SYBR®Green qPCR assays target the invA, rpoD, iroB and safC genes, as well ...

journal_title：Applied microbiology and biotechnology

authors： Barbau-Piednoir E,Bertrand S,Mahillon J,Roosens NH,Botteldoorn N

更新日期：2013-11-01 00:00:00

abstract：:Glucosylglycerols (GGs) are known as compatible solutes accumulated by some bacteria including cyanobacteria as well as higher plants for their adaptations to salt or desiccation stresses. Since being identified in Japanese sake, their physiological effects and potential applications on human health cares have been ex...

journal_title：Applied microbiology and biotechnology

authors： Tan X,Luo Q,Lu X

更新日期：2016-07-01 00:00:00

abstract：:Fluorescence in situ hybridization (FISH) employing nucleic acid mimics as probes is becoming an emerging molecular tool in the microbiology area for the detection and visualization of microorganisms. However, the impact that locked nucleic acid (LNA) and 2'-O-methyl (2'-OMe) RNA modifications have on the probe that i...

journal_title：Applied microbiology and biotechnology

authors： Fontenete S,Carvalho D,Guimarães N,Madureira P,Figueiredo C,Wengel J,Azevedo NF

更新日期：2016-07-01 00:00:00

abstract：:A cyclodextrin glucanotransferase (CGTase) gene of Bacillus ohbensis was cloned in Escherichia coli and the nucleotide sequence was determined. A single open reading frame (2112 bp) with a TTG codon as an initiator was identified that encodes a typical signal peptide of 29 amino acids followed by the mature enzyme (67...

journal_title：Applied microbiology and biotechnology

authors： Sin K,Nakamura A,Kobayashi K,Masaki H,Uozumi T

更新日期：1991-08-01 00:00:00

abstract：:A Lactobacillus brevis strain with the ability to synthesize butanol from glucose was constructed by metabolic engineering. The genes crt, bcd, etfB, etfA, and hbd, composing the bcs-operon, and the thl gene encode the enzymes of the lower part of the clostridial butanol pathway (crotonase, butyryl-CoA-dehydrogenase, ...

journal_title：Applied microbiology and biotechnology

authors： Berezina OV,Zakharova NV,Brandt A,Yarotsky SV,Schwarz WH,Zverlov VV

更新日期：2010-06-01 00:00:00

abstract：:The Vitreoscilla hemoglobin (VHb) gene (vgb) was integrated into the chromosome of Bacillus thuringiensis BMB171 using integrative vector pEG491. The production of VHb was confirmed by CO-difference spectra analysis. Fermentation experiments results showed that with the production of VHb, the critical oxygen concentra...

journal_title：Applied microbiology and biotechnology

authors： Liang F,Shouwen C,Ming S,Ziniu Y

更新日期：2007-02-01 00:00:00

abstract：:Tocopherols, collectively known as vitamin E, are lipophilic antioxidants, which are synthesized only by photosynthetic organisms. Due to their enormous potential to protect cells from oxidative damage, tocopherols are used, e.g., as nutraceuticals and additives in pharmaceuticals. The most biologically active form of...

journal_title：Applied microbiology and biotechnology

authors： Geipel K,Song X,Socher ML,Kümmritz S,Püschel J,Bley T,Ludwig-Müller J,Steingroewer J

更新日期：2014-03-01 00:00:00

abstract：:The lack of a universal method to extract RNA from soil hinders the progress of studies related to nitrification in soil, which is an important step in the nitrogen cycle. It is particularly difficult to extract RNA from certain types of soils such as Andosols (volcanic ash soils), which is the dominant agricultural s...

journal_title：Applied microbiology and biotechnology

authors： Wang Y,Nagaoka K,Hayatsu M,Sakai Y,Tago K,Asakawa S,Fujii T

更新日期：2012-11-01 00:00:00

abstract：:Cyanobacterial mutants defective in acyl-acyl carrier protein synthetase (Aas) secrete free fatty acids (FFAs) into the external medium and hence have been used for the studies aimed at photosynthetic production of biofuels. While the wild-type strain of Synechocystis sp. PCC 6803 is highly sensitive to exogenously ad...

journal_title：Applied microbiology and biotechnology

authors： Kojima K,Keta S,Uesaka K,Kato A,Takatani N,Ihara K,Omata T,Aichi M

更新日期：2016-12-01 00:00:00

abstract：:Synthetic biology aims at manipulating biological systems by rationally designed and genetically introduced components. Efforts in photoactuator engineering resulted in microorganisms reacting to extracellular light-cues with various cellular responses. Some of them lead to the formation of macroscopically observable ...

journal_title：Applied microbiology and biotechnology

authors： Renicke C,Taxis C

更新日期：2016-04-01 00:00:00

abstract：:We used experimental evolution in order to identify genes involved in the adaptation of Saccharomyces cerevisiae to the early stages of alcoholic fermentation. Evolution experiments were run for about 200 generations, in continuous culture conditions emulating the initial stages of wine fermentation. We performed whol...

journal_title：Applied microbiology and biotechnology

authors： Mangado A,Tronchoni J,Morales P,Novo M,Quirós M,Gonzalez R

更新日期：2015-02-01 00:00:00