Improved production of the insecticidal CryIVD protein in Bacillus thuringiensis using cryIA(c) promoters to express the gene for an associated 20-kDa protein.

abstract：:In order to understand the role of N1 domain (1-257 aa) in the amylopullulanase (gt-apu) of the extremely thermophilic bacterium Geobacillus thermoleovorans NP33, N1 deletion construct (gt-apuΔN) has been generated and expressed in Escherichia coli. The truncated amylopullulanase (gt-apuΔN) exhibits similar pH and tem...

journal_title：Applied microbiology and biotechnology

authors： Nisha M,Satyanarayana T

更新日期：2015-07-01 00:00:00

abstract：:In this study, the kinetic behaviors between acetone and composite bead were investigated. The microbial growth rate decreased with increasing average inlet concentration and increased with increasing operation temperature at average inlet concentration ranging from 50 to 300 ppm and operation temperature ranging from...

journal_title：Applied microbiology and biotechnology

authors： Chan WC,Chang LY

更新日期：2006-08-01 00:00:00

abstract：:The demand of renewable energy sources, i.e. biomass, is steadily increasing worldwide to reduce the need of fossil energy sources. Biomass such as energy crops, woody species, forestry and agricultural residues are the most common renewable energy sources. Due to uneven demand for wood fuel, the material is mostly st...

journal_title：Applied microbiology and biotechnology

authors： Noll M,Jirjis R

更新日期：2012-08-01 00:00:00

abstract：:Seven fractions rich in endoglucanase activity were separated from the extracellular cellulase system of the anaerobic rumen fungus Neocallimastix frontalis. The fractions (ES1, ES3, ES2U1, ES2U2, ES2U4, ES2U3C1 and ES2U3C2) were separated from each other and from a fraction that could solubilize crystalline cellulose...

journal_title：Applied microbiology and biotechnology

authors： Wood TM,Wilson CA,McCrae SI

更新日期：1995-12-01 00:00:00

abstract：:A maltotriose-producing α-amylase, AmyA, from a newly isolated bacterial strain Microbulbifer thermotolerans DAU221 was purified and characterized in the heterologous host, Escherichia coli, using the pCold I vector. The amyA gene encoded a 761-residue protein composed of a 33 amino acid secretion signal peptide. The ...

journal_title：Applied microbiology and biotechnology

authors： Lee YS,Park DJ,Choi YL

更新日期：2015-05-01 00:00:00

abstract：:Anaerobic biodegradation of atrazine by the bacterial isolate M91-3 was characterized with respect to mineralization, metabolite formation, and denitrification. The ability of the isolate to enhance atrazine biodegradation in anaerobic sediment slurries was also investigated. The organism utilized atrazine as its sole...

journal_title：Applied microbiology and biotechnology

authors： Crawford JJ,Sims GK,Mulvaney RL,Radosevich M

更新日期：1998-05-01 00:00:00

abstract：:Agar is a major cell wall carbohydrate of red macroalgae (Rhodophyta). Sugars derived from agar, such as agarooligosaccharides (AOSs), neoagarooligosaccharides (NAOSs), neoagarobiose (NAB), and 3,6-anhydro-L-galactose (L-AHG), possess various physiological activities. These agar-derived sugars can be produced by hydro...

journal_title：Applied microbiology and biotechnology

authors： Yun EJ,Yu S,Kim KH

更新日期：2017-07-01 00:00:00

abstract：:Actinobacteria, a large group of Gram-positive bacteria, secrete a wide range of extracellular enzymes involved in the degradation of organic compounds and biopolymers including the ubiquitous aminopolysaccharides chitin and chitosan. While chitinolytic enzymes are distributed in all kingdoms of life, actinobacteria a...

journal_title：Applied microbiology and biotechnology

authors： Lacombe-Harvey MÈ,Brzezinski R,Beaulieu C

更新日期：2018-09-01 00:00:00

abstract：:Microbes engineered to display heterologous proteins could be useful biotechnological tools for protein engineering, lignocellulose degradation, biocatalysis, bioremediation, and biosensing. Bacillus subtilis is a promising host to display proteins, as this model Gram-positive bacterium is genetically tractable and al...

journal_title：Applied microbiology and biotechnology

authors： Huang GL,Gosschalk JE,Kim YS,Ogorzalek Loo RR,Clubb RT

更新日期：2018-08-01 00:00:00

abstract：:This research develops a safe, inexpensive, and more accessible source for synthesis of silver nanoparticles. The bioactive silver composites synthesized by Lactococcus lactis 56 KY484989 (LCLB56-AgCs) were characterized by various physico-chemical techniques and investigated for their antimicrobial activity and cytot...

journal_title：Applied microbiology and biotechnology

authors： Viorica RP,Pawel P,Kinga M,Michal Z,Katarzyna R,Boguslaw B

更新日期：2017-10-01 00:00:00

abstract：:Previous work showed that random mutagenesis produced a mutant of toluene ortho-monooxygenase (TOM) of Burkholderia cepacia G4 containing the V106A substitution in the hydroxylase alpha-subunit (TomA3) that changed the color of the cell suspension from wild-type brown to green in rich medium. Here, DNA shuffling was u...

journal_title：Applied microbiology and biotechnology

authors： Rui L,Reardon KF,Wood TK

更新日期：2005-01-01 00:00:00

abstract：:A recent study of the UV-generated glucose-utilizing mutant Ralstonia eutropha G⁺1 comprising transcriptomic and proteomic analyses revealed clear evidence that glucose is transported by the N-acetylglucosamine-specific phosphotransferase system (PTS(Nag)), which is overexpressed in this mutant due to a derepression o...

journal_title：Applied microbiology and biotechnology

authors： Raberg M,Kaddor C,Kusian B,Stahlhut G,Budinova R,Kolev N,Bowien B,Steinbüchel A

更新日期：2012-08-01 00:00:00

abstract：:During the process of producing cassava starch from Manihot esculenta roots, large amounts of cyanoglycosides were released, which rapidly decayed to CN- following enzymatic hydrolysis. Depending on the varying cyanoglycoside content of the cassava varieties, the cyanide concentration in the wastewater was as high as ...

journal_title：Applied microbiology and biotechnology

authors： Siller H,Winter J

更新日期：1998-02-01 00:00:00

abstract：:The genus Aspergillus is widely used for the production of plant cell wall polysaccharide-degrading enzymes. The range of enzymes purified from these fungi covers nearly every function required for the complete degradation of cellulose, xyloglucan, xylan, galacto(gluco)mannan and pectin. This paper describes the Asper...

journal_title：Applied microbiology and biotechnology

authors： de Vries RP

更新日期：2003-03-01 00:00:00

abstract：:Streptomyces tendae Tü 901 produces the nucleoside peptide antibiotic nikkomycin. In shot-gun cloning experiments using pIJ699 as vector we isolated a 9.4-kb DNA fragment from S. tendae which complemented the nikkomycin nonproducing mutant NP9 to the formation of nikkomycin C/Cx and Kx. Nikkomycins were identified by ...

journal_title：Applied microbiology and biotechnology

authors： Bormann C,Aberle K,Fiedler HP,Schrempf H

更新日期：1990-01-01 00:00:00

abstract：:Rhodococcus erythropolis U23A is a polychlorinated biphenyl (PCB)-degrading bacterium isolated from the rhizosphere of plants grown on a PCB-contaminated soil. Strain U23A bphA exhibited 99% identity with bphA1 of Rhodococcus globerulus P6. We grew Arabidopsis thaliana in a hydroponic axenic system, collected, and con...

journal_title：Applied microbiology and biotechnology

authors： Toussaint JP,Pham TT,Barriault D,Sylvestre M

更新日期：2012-09-01 00:00:00

abstract：:Our previous work showed that there is a limitation in the use of dihydrofolate reductase (dhfr)/methotrexate (MTX)-mediated gene amplification systems in dhfr-non-deficient HEK293 cells, as endogenous dhfr may interfere with the amplification process. In the present study, we successfully generated Epstein-Barr virus...

journal_title：Applied microbiology and biotechnology

authors： Park SH,Park JH,Lee JH,Lee HM,Kang YJ,Lee EJ,Shin S,Lee GM,Kim YG

更新日期：2021-01-01 00:00:00

abstract：:This study aimed to investigate the interaction between methane production performance and active microbial community dynamics at different loading rates by increasing influent substrate concentration. The model system was an upflow anaerobic sludge blanket (UASB) reactor using molasses wastewater. The active microbia...

journal_title：Applied microbiology and biotechnology

authors： Yun J,Lee SD,Cho KS

更新日期：2016-05-01 00:00:00

abstract：:Termitomyces is a genus of edible mushrooms commonly consumed in Africa and Asia among the mushrooms collected from the wild. Termitomyces mushrooms grow as symbionts in the termite nests, where they produce various enzymes to help termites digest lignocellulosic substrates. Many species of Termitomyces are used by di...

journal_title：Applied microbiology and biotechnology

authors： Hsieh HM,Ju YM

更新日期：2018-06-01 00:00:00

abstract：:Several studies support the use of probiotics for the treatment of minor gastrointestinal problems in infants. Positive effects on newborn colics have been evidenced after administration of Lactobacillus strains, whereas no studies have been reported regarding the use of bifidobacteria for this purpose. This work was ...

journal_title：Applied microbiology and biotechnology

authors： Aloisio I,Santini C,Biavati B,Dinelli G,Cencič A,Chingwaru W,Mogna L,Di Gioia D

更新日期：2012-12-01 00:00:00

abstract：:This study presents a novel methodology for the development of a chemically defined medium (CDM) using genome-scale metabolic network and flux balance analysis. The genome-based in silico analysis identified two amino acids and four vitamins as non-substitutable essential compounds to be supplemented to a minimal medi...

journal_title：Applied microbiology and biotechnology

authors： Song H,Kim TY,Choi BK,Choi SJ,Nielsen LK,Chang HN,Lee SY

更新日期：2008-05-01 00:00:00

abstract：:Mycorrhizal fungi belonging to the genus Tuber produce, after the establishment of a productive interaction with a plant host, hypogeous fruitbodies of great economic value known as ''truffles''. This review summarizes the state of art on life cycle, genetic, and biotechnological investigations of Tuber spp. The ascoc...

journal_title：Applied microbiology and biotechnology

authors： Poma A,Limongi T,Pacioni G

更新日期：2006-09-01 00:00:00

abstract：:Oxygenates, mainly methyl tert-butyl ether (MTBE), are commonly added to gasoline to enhance octane index and improve combustion efficiency. Other oxygenates used as gasoline additives are ethers such as ethyl tert-butyl ether (ETBE), tert-amyl methyl ether (TAME), and alcohols such as tert-butyl alcohol (TBA). As a r...

journal_title：Applied microbiology and biotechnology

authors： Fayolle F,Vandecasteele JP,Monot F

更新日期：2001-08-01 00:00:00

abstract：:A strong promoter increases transcription of the genes of interest and enhances the production of various valuable substances. For a hyperthermophilic archaeon Thermococcus onnurineus NA1, which can produce H2 from carbon monoxide oxidation, we searched for a novel endogenous strong promoter by transcriptome analysis ...

journal_title：Applied microbiology and biotechnology

authors： Lee SH,Kim MS,Jung HC,Lee J,Lee JH,Lee HS,Kang SG

更新日期：2015-05-01 00:00:00

abstract：:Cells of an aerobic three-membered bacterial co-culture, designated as ECO3, capable of cometabolizing and aerobically dechlorinating low-chlorinated biphenyls in the presence of biphenyl, were immobilized on Manville silica beads, on frosted-glass beads and on polyurethane foam cubes in packed-bed bioreactors continu...

journal_title：Applied microbiology and biotechnology

authors： Fava F,Di Gioia D,Marchetti L,Quattroni G

更新日期：1996-05-01 00:00:00

abstract：:A cDNA fragment encoding the A catalytic domain of the Neocallimastix frontalis endoxylanase XYN3 was amplified and cloned by the polymerase chain reaction technique. The xyn3A DNA fragment was inserted between the Saccharomyces cerevisiae phosphoglycerate kinase gene promoter and terminator sequences on a multicopy e...

journal_title：Applied microbiology and biotechnology

authors： Durand R,Rascle C,Fèvre M

更新日期：1999-08-01 00:00:00

abstract：:The aim of this study was to elucidate the kinetic constraints during the redox biotransformation of the azo dye, Reactive Red 2 (RR2), and carbon tetrachloride (CT) mediated by soluble humic acids (HAs) and immobilized humic acids (HAi), as well as by the quinoid model compounds, anthraquinone-2,6-disulfonate (AQDS) ...

journal_title：Applied microbiology and biotechnology

authors： Cervantes FJ,Martínez CM,Gonzalez-Estrella J,Márquez A,Arriaga S

更新日期：2013-03-01 00:00:00

abstract：:The nutritional medium requirement for biomass and triterpenoid production by Antrodia cinnamomea AC0623 strain was optimized. Box-Behnken was applied to optimize biomass and triterpenoid production. According to response surface methodology (RSM), the optimum concentrations of N-source were determined. The results in...

journal_title：Applied microbiology and biotechnology

authors： Chang CY,Lee CL,Pan TM

更新日期：2006-10-01 00:00:00

abstract：:Soluble expression of recombinant therapeutic proteins in Escherichia coli (E. coli) has been a challenging task in biopharmaceutical development. In this study, a novel self-cleavable tag Zbasic-intein has been constructed for the soluble expression and purification of a recombinant cytokine, human interleukin-15 (IL...

journal_title：Applied microbiology and biotechnology

authors： Shi S,Chen H,Jiang H,Xie Y,Zhang L,Li N,Zhu C,Chen J,Luo H,Wang J,Feng L,Lu H,Zhu J

更新日期：2017-02-01 00:00:00

abstract：:This study reports enzymatic hydrolysis of the biomass of the giant reed (Arundo donax L.) after ammonia fibre expansion (AFEX) pretreatment. In particular, the capacity of the arabinofuranosidase from the fungus Pleurotus ostreatus recombinantly expressed in Pichia pastoris rPoAbf, its evolved mutant rPoAbf F435Y/Y44...

journal_title：Applied microbiology and biotechnology

authors： Giacobbe S,Balan V,Montella S,Fagnano M,Mori M,Faraco V

更新日期：2016-03-01 00:00:00