Expression of a catalytic domain of a Neocallimastix frontalis endoxylanase gene (xyn3) in Kluyveromyces lactis and Penicillium roqueforti.

abstract：:Extracellular polymeric substances (EPS) play an important role in the formation and activity of biofilms in wastewater treatment (WWT). The EPS of the denitrifying biomarker Comamonas denitrificans strain 110, produced in different culture media and growth modes, were characterized. The EPS mainly contained protein (...

journal_title：Applied microbiology and biotechnology

authors： Andersson S,Dalhammar G,Land CJ,Kuttuva Rajarao G

更新日期：2009-03-01 00:00:00

abstract：:Xylanase is the enzyme complex that is responsible for the degradation of xylan; however, novel xylanase producers remain to be explored in marine environment. In this study, a Streptomyces strain M11 which exhibited xylanase activity was isolated from marine sediment. The 16S rDNA sequence of M11 showed the highest i...

journal_title：Applied microbiology and biotechnology

authors： Liu Z,Zhao X,Bai F

更新日期：2013-05-01 00:00:00

abstract：:In oyster ponds, the marine diatom Haslea ostrearia synthesises and excretes a hydrosoluble pigment of commercial interest called marennin. During the benthic stage, when algal cells are naturally immobilised in their own polysaccharides, marennin production is higher. To optimise this production, axenic cultures of H...

journal_title：Applied microbiology and biotechnology

authors： Lebeau T,Gaudin P,Moan R,Robert JM

更新日期：2002-07-01 00:00:00

abstract：:Twenty-six species of Phellinus sensu lato, reported as medicinal mushrooms, are enumerated in this review. The species' names were checked and revised according to contemporary taxonomy and the latest version of the International Code of Botanical Nomenclature (Vienna Code); two misapplied names of Phellinus baumii P...

journal_title：Applied microbiology and biotechnology

authors： Dai YC,Zhou LW,Cui BK,Chen YQ,Decock C

更新日期：2010-08-01 00:00:00

abstract：:Cytidine diphosphate choline (CDP-choline) has been applied for treating acute craniocerebral injury and allowing recovery of consciousness after brain surgery. In this study, an acetate kinase (ACK)/acetyl phosphate system was used to supply ATP and combined with Escherichia coli-overexpressed CMP kinase (CMK), NDP k...

journal_title：Applied microbiology and biotechnology

authors： Liu Y,Wang J,Xu C,Chen Y,Yang J,Liu D,Niu H,Jiang Y,Yang S,Ying H

更新日期：2017-02-01 00:00:00

abstract：:The production of acetone, butanol and ethanol by fermentation of renewable biomass has potential to become a valuable industrial process. Mechanisms of solvent production and sporulation involve some common regulators in some ABE-producing clostridia, although details of the links between the pathways are not clear. ...

journal_title：Applied microbiology and biotechnology

authors： Kolek J,Diallo M,Vasylkivska M,Branska B,Sedlar K,López-Contreras AM,Patakova P

更新日期：2017-11-01 00:00:00

abstract：:Extracellular manganese peroxidase (MnP) was purified from the compost extract of Agaricus bisporus using anion exchange chromatography, gel filtration and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Two forms (MnP1 and MnP2) were separated by isoelectric focusing and their isoelectric points...

journal_title：Applied microbiology and biotechnology

authors： Lankinen VP,Bonnen AM,Anton LH,Wood DA,Kalkkinen N,Hatakka A,Thurston CF

更新日期：2001-03-01 00:00:00

abstract：:Acid tolerance and aroma profile are crucial factors for wine production in Saccharomyces cerevisiae. However, most wine yeasts to date fail to endure low-pH environments, therefore resulting in problems such as lengthened fermentation and poor flavor during acidic fruit wine production. In the present study, we estab...

journal_title：Applied microbiology and biotechnology

authors： Tian T,Wu D,Ng CT,Yang H,Sun J,Liu J,Lu J

更新日期：2020-04-01 00:00:00

abstract：:The helix-loop-helix (HLH) family of transcriptional factors is a key player in a wide range of developmental processes in organisms from mammals to microbes. We previously identified the bHLH transcription factor SclR in Aspergillus oryzae and found that the loss of SclR function led to significant phenotypic changes...

journal_title：Applied microbiology and biotechnology

authors： Jin FJ,Han P,Zhuang M,Zhang ZM,Jin L,Koyama Y

更新日期：2018-01-01 00:00:00

abstract：:Stirred, pH-controlled anaerobic batch cultures were used to evaluate the in vitro utilisation by canine gut microflora of novel alpha-galactooligosaccharides synthesised with an enzyme extract from a canine Lactobacillus reuteri strain. Fructooligosaccharides (FOS), melibiose and raffinose were used as reference carb...

journal_title：Applied microbiology and biotechnology

authors： Tzortzis G,Goulas AK,Baillon ML,Gibson GR,Rastall RA

更新日期：2004-03-01 00:00:00

abstract：:To measure the toxicity experienced by superoxide-generating compounds, two plasmids were constructed in which the superoxide-inducible fpr and zwf promoters from Escherichia coli were fused to promoterless Vibrio fischeri luxCDABE operon present in plasmid pUCD615. The bioluminescent response of E. coli harboring the...

journal_title：Applied microbiology and biotechnology

authors： Niazi JH,Kim BC,Gu MB

更新日期：2007-04-01 00:00:00

abstract：:Both aerobic methane-oxidizing bacteria (MOB) and nitrite-dependent anaerobic methane oxidation (n-damo) bacteria can play an important role in mitigating the methane emission produced in anoxic sediment layers to the atmosphere. However, the environmental factors regulating the distribution of these methane-oxidizing...

journal_title：Applied microbiology and biotechnology

authors： Liu Y,Zhang J,Zhao L,Li Y,Yang Y,Xie S

更新日期：2015-03-01 00:00:00

abstract：:In this study, we employed TiO₂ enrichment and high accuracy liquid chromatography-mass spectrometry-mass spectrometry to identify the phosphoproteome of Clostridium acetobutyicum ATCC824 in acidogenesis and solventogenesis. As many as 82 phosphopeptides in 61 proteins, with 107 phosphorylated sites on serine, threoni...

journal_title：Applied microbiology and biotechnology

authors： Bai X,Ji Z

更新日期：2012-07-01 00:00:00

abstract：:A freshwater algicidal bacterial strain, Lzh-5, isolated from Lake Taihu, with strong algicidal activity against Microcystis aeruginosa, was identified as Bacillus sp. based on its phenotypic characteristics and 16S ribosomal RNA (rRNA) gene sequence. The algicidal mode of Bacillus sp. Lzh-5 was indirect, attacking M....

journal_title：Applied microbiology and biotechnology

authors： Li Z,Geng M,Yang H

更新日期：2015-01-01 00:00:00

abstract：:Trehalose (α-D-glucopyranosyl-(1 → 1)-α-D-glucopyranoside) is a non-reducing disaccharide composed of two glucose molecules linked by an α,α-1,1-glycosidic bond. It possesses physicochemical properties, which account for its biological roles in a variety of prokaryotic and eukaryotic organisms and invertebrates. Inten...

journal_title：Applied microbiology and biotechnology

authors： Cai X,Seitl I,Mu W,Zhang T,Stressler T,Fischer L,Jiang B

更新日期：2018-04-01 00:00:00

abstract：:A drawback of biotechnological processes, where microorganisms convert biomass constituents, such as starch, cellulose, hemicelluloses, lipids, and proteins, into wanted products, is the economic feasibility. Particularly the cost of nitrogen sources in biotechnological processes can make up a large fraction of total ...

journal_title：Applied microbiology and biotechnology

authors： Pleissner D,Venus J

更新日期：2016-03-01 00:00:00

abstract：:Forty-eight single-use stirred tank bioreactors on a 10-mL scale operated in a magnetically inductive driven bioreaction block and automated with a liquid handler were applied for discrimination of different riboflavin producing Bacillus subtilis strains based on their performances in the parallel fed-batch processes....

journal_title：Applied microbiology and biotechnology

authors： Vester A,Hans M,Hohmann HP,Weuster-Botz D

更新日期：2009-08-01 00:00:00

abstract：:The human intestine hosts various complex microbial communities that are closely associated with multiple health and disease processes. Determining the composition and function of these microbial communities is critical to unveil disease mechanisms and promote human health. Recently, meta-omic strategies have been dev...

journal_title：Applied microbiology and biotechnology

authors： Xiao M,Yang J,Feng Y,Zhu Y,Chai X,Wang Y

更新日期：2017-04-01 00:00:00

abstract：:Deoxynucleoside-5'-monophosphates (5'-dNMPs) are the basic components of DNA and are widely used in medicine and as chemical and biochemical reagents. A large amount of effort has been expended to obtain 5'-dNMPs of high quality and at a low cost. However, these procedures are inefficient and inconvenient. In this stu...

journal_title：Applied microbiology and biotechnology

authors： Zou Z,Ding Q,Ou L,Yan B

更新日期：2013-11-01 00:00:00

abstract：:Respiration measurement in shake flasks is introduced as a new method to characterize the metabolic activity of microorganisms during and after stress exposure. The major advantage of the new method is the possibility to determine the metabolic activity independent of manual sampling without the necessity to change th...

journal_title：Applied microbiology and biotechnology

authors： Seletzky JM,Noack U,Fricke J,Hahn S,Büchs J

更新日期：2006-10-01 00:00:00

abstract：:A Lactobacillus brevis strain with the ability to synthesize butanol from glucose was constructed by metabolic engineering. The genes crt, bcd, etfB, etfA, and hbd, composing the bcs-operon, and the thl gene encode the enzymes of the lower part of the clostridial butanol pathway (crotonase, butyryl-CoA-dehydrogenase, ...

journal_title：Applied microbiology and biotechnology

authors： Berezina OV,Zakharova NV,Brandt A,Yarotsky SV,Schwarz WH,Zverlov VV

更新日期：2010-06-01 00:00:00

abstract：:Mycelial adhesion affects cell growth and the production of water-soluble extracellular yellow pigment (EYP) in submerged fermentation with Monascus ruber CGMCC 10910. Two nitrates, NaNO3 and KNO3, were used as nitrogen sources for mitigating mycelial adhesion and improving the production of EYP in this study. The res...

journal_title：Applied microbiology and biotechnology

authors： Yang SZ,Huang ZF,Liu HQ,Hu X,Wu ZQ

更新日期：2020-11-01 00:00:00

abstract：:Poly(hydroxyalkanoates) (PHAs) constitute biodegradable polyesters and are considered among the most promising candidates to replace common petrochemical plastics in various applications. To date, all commercial processes for PHA production employ microbial discontinuous fed-batch fermentations. These processes featur...

journal_title：Applied microbiology and biotechnology

authors： Atlić A,Koller M,Scherzer D,Kutschera C,Grillo-Fernandes E,Horvat P,Chiellini E,Braunegg G

更新日期：2011-07-01 00:00:00

abstract：:A study was conducted to optimize the biodegradation in soil slurries of phenanthrene initially dissolved in nonaqueous-phase liquids (NAPLs). The slow rate of degradation of phenanthrene in dibutyl phthalate was increased by addition of phenanthrene-degrading microorganisms to soil slurries containing the NAPL. The r...

journal_title：Applied microbiology and biotechnology

authors： Birman I,Alexander M

更新日期：1996-03-01 00:00:00

abstract：:The unicellular cyanobacterium Synechocystis sp. PCC6714 can grow not only under photoautotrophic conditions, but also under chemoheterotrophic conditions if glucose is added to the medium. This makes it useful for the study of many aspects of bioenergetic mechanisms. In contrast to its closely related strain Synechoc...

journal_title：Applied microbiology and biotechnology

authors： Ludwig A,Heimbucher T,Gregor W,Czerny T,Schmetterer G

更新日期：2008-03-01 00:00:00

abstract：:Recently, several endophytic fungi have been demonstrated to produce volatile organic compounds (VOCs) with properties similar to fossil fuels, called "mycodiesel," while growing on lignocellulosic plant and agricultural residues. The fact that endophytes are plant symbionts suggests that some may be able to produce l...

journal_title：Applied microbiology and biotechnology

authors： Wu W,Davis RW,Tran-Gyamfi MB,Kuo A,LaButti K,Mihaltcheva S,Hundley H,Chovatia M,Lindquist E,Barry K,Grigoriev IV,Henrissat B,Gladden JM

更新日期：2017-03-01 00:00:00

abstract：:Corynebacterium glutamicum owns a citrate synthase and two methylcitrate synthases. Characterization of the isolated enzymes showed that the two methylcitrate synthases have comparable catalytic efficiency, k (cat)/K (m), as the citrate synthase with acetyl-CoA as substrate, although these enzymes are only synthesized...

journal_title：Applied microbiology and biotechnology

authors： Radmacher E,Eggeling L

更新日期：2007-09-01 00:00:00

abstract：:Wild-type naphthalene dioxygenase (NDO) from Pseudomonas sp. strain NCIB 9816-4 transforms relatively planar flavone and isoflavone to cis-dihydrodiols. However, this enzyme cannot catalyze the transformation of flavanone and isoflavanone in which a phenyl group bonds to the stereogenic C2 or C3 of the C-ring. Protein...

journal_title：Applied microbiology and biotechnology

authors： Seo J,Ryu JY,Han J,Ahn JH,Sadowsky MJ,Hur HG,Chong Y

更新日期：2013-01-01 00:00:00

abstract：:Site-saturation mutagenesis (SSM) has been used in directed evolution of proteins for a long time. As a special form of saturation mutagenesis, it involves individual randomization at a given residue with formation of all 19 amino acids. To date, the most efficient embodiment of SSM is a one-step PCR-based approach us...

journal_title：Applied microbiology and biotechnology

authors： Li A,Acevedo-Rocha CG,Reetz MT

更新日期：2018-07-01 00:00:00

abstract：:Porcine parvovirus type 1 (PPV1) is a major causative agent of embryonic and fetal death in swine. The PPV1 VP2 protein is closely associated with viral immunogenicity for eliciting neutralizing antibodies, but its antigenic structures have been largely unknown. We generated three monoclonal antibodies (MAbs) against ...

journal_title：Applied microbiology and biotechnology

authors： Sun J,Huang L,Wei Y,Wang Y,Chen D,Du W,Wu H,Feng L,Liu C

更新日期：2015-11-01 00:00:00