Identification of a chitosanase from the marine metagenome and its molecular improvement based on evolution data.

abstract：:To optimize the expression of cry genes in a Bacillus thuringiensis sigK mutant failing in crystal releasing, the transcriptional activity of the cry promoters cry1A, cry3A, cry4A, and cry8E was compared using lacZ gene fusions. A beta-galactosidase assay indicated that the cry8E promoter showed the highest transcript...

journal_title：Applied microbiology and biotechnology

authors： Zhou C,Zheng Q,Peng Q,Du L,Shu C,Zhang J,Song F

更新日期：2014-09-01 00:00:00

abstract：:Cultures of Umbelopsis ramanniana (=Mucor ramannianus) were grown in fluid Sabouraud medium for 3 days, dosed with 0.23 mM benzo[f]quinoline, benzo[h]quinoline, or phenanthridine (benzo[c]quinoline), and incubated for another 18 days. Cultures were extracted and metabolites (66-75% of the UV absorbance) were separated...

journal_title：Applied microbiology and biotechnology

authors： Sutherland JB,Cross EL,Heinze TM,Freeman JP,Moody JD

更新日期：2005-05-01 00:00:00

abstract：:The phenomenon of the cell density effect is not readily explained by an obvious nutrient limitation, and a recent study has suggested that for recombinant Autographa californica multiple nucleopolyhedrovirus (rAcMNPV)-infected Sf9 cells, a drop in messenger RNA (mRNA) levels may be sufficient to explain the cell dens...

journal_title：Applied microbiology and biotechnology

authors： Huynh HT,Tran TT,Chan LC,Nielsen LK,Reid S

更新日期：2015-02-01 00:00:00

abstract：:1,3-Propanediol (1,3-PD) production by fermentation of glycerol was described in 1881 but little attention was paid to this microbial route for over a century. Glycerol conversion to 1,3-PD can be carried out by Clostridia as well as Enterobacteriaceae. The main intermediate of the oxidative pathway is pyruvate, the f...

journal_title：Applied microbiology and biotechnology

authors： Biebl H,Menzel K,Zeng AP,Deckwer WD

更新日期：1999-09-01 00:00:00

abstract：:D-allose has attracted a great deal of attention in recent years due to its many pharmaceutical activities, which include anti-cancer, anti-tumor, anti-inflammatory, anti-oxidative, anti-hypertensive, cryoprotective, and immunosuppressant activities. D-allose has been produced from D-psicose using D-allose-producing e...

journal_title：Applied microbiology and biotechnology

authors： Lim YR,Oh DK

更新日期：2011-07-01 00:00:00

abstract：:The aim of the study was to investigate whether toxic fine chemical production can be improved using the solvent-tolerant Pseudomonas putida S12 in a two-liquid-phase system consisting of aqueous media and a water-immiscible octanol phase with production of 3-methylcatechol from toluene as the model conversion. For th...

journal_title：Applied microbiology and biotechnology

authors： Wery J,Mendes da Silva DI,de Bont JA

更新日期：2000-08-01 00:00:00

abstract：:It has been well documented that Aureobasidium pullulans is widely distributed in different environments. Different strains of A. pullulans can produce amylase, proteinase, lipase, cellulase, xylanase, mannanase, transferases, pullulan, siderophore, and single-cell protein, and the genes encoding proteinase, lipase, c...

journal_title：Applied microbiology and biotechnology

authors： Chi Z,Wang F,Chi Z,Yue L,Liu G,Zhang T

更新日期：2009-04-01 00:00:00

abstract：:A freshwater algicidal bacterial strain, Lzh-5, isolated from Lake Taihu, with strong algicidal activity against Microcystis aeruginosa, was identified as Bacillus sp. based on its phenotypic characteristics and 16S ribosomal RNA (rRNA) gene sequence. The algicidal mode of Bacillus sp. Lzh-5 was indirect, attacking M....

journal_title：Applied microbiology and biotechnology

authors： Li Z,Geng M,Yang H

更新日期：2015-01-01 00:00:00

abstract：:Two bioactive O-methylflavonoids, sakuranetin (7-O-methylnaringenin) and ponciretin (7-O-methylnaringenin), were synthesized in Escherichia coli. Sakuranetin inhibits germination of Magnaporthe grisea, and ponciretin is a potential inhibitor of Helicobacter pylori. To achieve this, we reconstructed the naringenin bios...

journal_title：Applied microbiology and biotechnology

authors： Kim MJ,Kim BG,Ahn JH

更新日期：2013-08-01 00:00:00

abstract：:This review describes the historical development and current state of metals leaching and sulfide mineral biooxidation by the minerals industries. During the past 20 years commercial processes employing microorganisms for mineral recovery have progressed from rather uncontrolled copper dump leaching to mineral oxidati...

journal_title：Applied microbiology and biotechnology

authors： Olson GJ,Brierley JA,Brierley CL

更新日期：2003-12-01 00:00:00

abstract：:Immobilization of the Geotrichum candidum (CCT 1205) cell with functionalized silica creates promising biocatalysts for production of ɛ-caprolactone. The results obtained by immobilization of the whole cell on SiO2-NH2 and SiO2-SH supports indicate that the presence of reactive functional groups on the support may pro...

journal_title：Applied microbiology and biotechnology

authors： Silva ALP,da Silva Caridade TN,Magalhães RR,de Sousa KT,de Sousa CC,Vale JA

更新日期：2020-10-01 00:00:00

abstract：:Pyranose 2-oxidase (P2O) was purified 43-fold to apparent homogeneity from the basidiomycete Phanerochaete chrysosporium using liquid chromatography on phenyl Sepharose, Mono Q (twice) and phenyl Superose. The native enzyme has a molecular mass of about 250 kDa (based on native PAGE) and is composed of four identical ...

journal_title：Applied microbiology and biotechnology

authors： Artolozaga MJ,Kubátová E,Volc J,Kalisz HM

更新日期：1997-05-01 00:00:00

abstract：:In order for microalgae-based processes to reach commercial production for biofuels and high-value products such as omega-3 fatty acids, it is necessary that economic feasibility be demonstrated at the industrial scale. Therefore, process optimization is critical to ensure that the maximum yield can be achieved from t...

journal_title：Applied microbiology and biotechnology

authors： Lowrey J,Armenta RE,Brooks MS

更新日期：2016-02-01 00:00:00

abstract：:Overuse of broad-spectrum antibiotics to control human and plant pathogens greatly accelerated the development of antibiotic resistance among bacteria and fungi. Therefore, usage of new approaches is necessary to control outbreaks of phytopathogenic diseases as well as multidrug-resistant human pathogens. Many of the ...

journal_title：Applied microbiology and biotechnology

authors： Olishevska S,Nickzad A,Déziel E

更新日期：2019-02-01 00:00:00

abstract：:Staphylococcus aureus is a pathogen responsible for severe community- and nosocomially acquired infections. To fight pathogen intrusion, the innate immune system uses a plethora of weapons, with the generation of oxidative and nitrosative stresses among the most efficient. In this work, the S. aureus genome-wide trans...

journal_title：Applied microbiology and biotechnology

authors： Nobre LS,Saraiva LM

更新日期：2013-03-01 00:00:00

abstract：:The effect of sodium chloride (NaCl) on pullulan production by batch culture of Aureobasidium pullulans CCTCC M 2012259 was investigated. NaCl at 3 g/L improved the pullulan titer by 26.7% but reduced the molecular weight of pullulan to only 46.8% of that obtained in the control without NaCl. In order to elucidate the...

journal_title：Applied microbiology and biotechnology

authors： Wang DH,Ni TF,Ju XM,Wei GY

更新日期：2018-10-01 00:00:00

abstract：:Leuconostoc carnosum 4010 is a protective culture for meat products. It kills the foodborne pathogen Listeria monocytogenes by producing two class IIa (pediocin-like) bacteriocins, leucocin A and leucocin C. The genes for leucocin A production have previously been characterised from Leuconostoc gelidum UAL 187, wherea...

journal_title：Applied microbiology and biotechnology

authors： Wan X,Li R,Saris PE,Takala TM

更新日期：2013-04-01 00:00:00

abstract：:Shikimic acid is an industrially important chiral compound used as a key ingredient in formulation of drug Oseltamivir phosphate (Tamiflu) for the treatment of swine/avian flu. The high cost and limited availability of shikimic acid isolated from plants has detained the use of this valuable building block of the drug....

journal_title：Applied microbiology and biotechnology

authors： Rawat G,Tripathi P,Saxena RK

更新日期：2013-05-01 00:00:00

abstract：:To clarify the effect of type of foods on the intestinal environment, Far East Asian- (FEA; rich in rice starch, soy protein and soy oil) and Far East Asian marine- (FEAM; rich in rice starch, fish meal, fish oil and brown alga) modelled diets and sucrose, casein and beef tallow-rich (SCB) diet were prepared. After th...

journal_title：Applied microbiology and biotechnology

authors： An C,Kuda T,Yazaki T,Takahashi H,Kimura B

更新日期：2014-05-01 00:00:00

abstract：:Bionanocapsules (BNCs) are hollow nanoscale particles composed of L protein of the hepatitis B virus surface antigen that represent specific affinity for human hepatocytes. BNCs can transfer genes and drugs into human hepatocytes efficiently and specifically. BNC can be expressed in yeast cells. In this study, we deve...

journal_title：Applied microbiology and biotechnology

authors： Shishido T,Muraoka M,Ueda M,Seno M,Tanizawa K,Kuroda S,Fukuda H,Kondo A

更新日期：2006-12-01 00:00:00

abstract：:Plants as well as pro- and eukaryotic microorganisms are able to synthesise lysine via de novo synthesis. While plants and bacteria, with some exceptions, rely on variations of the meso-diaminopimelate pathway for lysine biosynthesis, fungi exclusively use the α-aminoadipate pathway. Although bacteria and fungi are, i...

journal_title：Applied microbiology and biotechnology

authors： Fazius F,Zaehle C,Brock M

更新日期：2013-05-01 00:00:00

abstract：:The effects of Bacillus sp. SLS18, a plant-growth-promoting endophyte, on the biomass production and Mn/Cd uptake of sweet sorghum (Sorghum bicolor L.), Phytolacca acinosa Roxb., and Solanum nigrum L. were investigated. SLS18 displayed multiple heavy metals and antibiotics resistances. The strain also exhibited the ca...

journal_title：Applied microbiology and biotechnology

authors： Luo S,Xu T,Chen L,Chen J,Rao C,Xiao X,Wan Y,Zeng G,Long F,Liu C,Liu Y

更新日期：2012-02-01 00:00:00

abstract：:Panax ginseng C.A. Meyer (ginseng) is a well-known medicinal plant that has been traditionally used in the oriental countries for centuries. Wild ginseng is a scarce and rare commodity. Field cultivation of the ginseng plant is a time-consuming and labor-intensive process. Ginsenosides, a group of glycosylated triterp...

journal_title：Applied microbiology and biotechnology

authors： Murthy HN,Georgiev MI,Kim YS,Jeong CS,Kim SJ,Park SY,Paek KY

更新日期：2014-01-01 00:00:00

abstract：:Photothermal cancer therapy, as a prospective approach for local cancer treatment, is attracting increasing interests. In this paper, gold nanorods were conjugated with folate (folate/AuNRs), and their photothermal effects on hepatocellular carcinoma cell line (HepG2) using MTT assay, flow cytometry, as well as on the...

journal_title：Applied microbiology and biotechnology

authors： Jin H,Yang P,Cai J,Wang J,Liu M

更新日期：2012-06-01 00:00:00

abstract：:Oxygenates, mainly methyl tert-butyl ether (MTBE), are commonly added to gasoline to enhance octane index and improve combustion efficiency. Other oxygenates used as gasoline additives are ethers such as ethyl tert-butyl ether (ETBE), tert-amyl methyl ether (TAME), and alcohols such as tert-butyl alcohol (TBA). As a r...

journal_title：Applied microbiology and biotechnology

authors： Fayolle F,Vandecasteele JP,Monot F

更新日期：2001-08-01 00:00:00

abstract：:Dialysis techniques are discussed as a means for effective removal of low-molecular-mass components from fermentation broth to reach high cell density. Reactor systems and process strategies, the relevant properties of membranes and examples for high-density fermentation with dialysis, and problems related to scale-up...

journal_title：Applied microbiology and biotechnology

authors： Pörtner R,Märkl H

更新日期：1998-10-01 00:00:00

abstract：:Escherichia coli K-12 was cultured under anaerobic conditions to form biofilm on carbon fiber electrodes in glucose-containing medium. The anodic current increased with the development of the biofilm and depended on the glucose concentration. Cyclic voltammetric results support the presence of a redox compound(s) excr...

journal_title：Applied microbiology and biotechnology

authors： Wang YF,Tsujimura S,Cheng SS,Kano K

更新日期：2007-10-01 00:00:00

abstract：:This paper reports the in vivo expression of the synthetic transposase gene tnp(a) from a hyperactive Tn5 tnp gene mutant in Streptomyces coelicolor. Using the synthetic tnp(a) gene adapted for Streptomyces codon usage, we showed random insertion of the transposon into the Streptomycetes genome. The insertion frequenc...

journal_title：Applied microbiology and biotechnology

authors： Petzke L,Luzhetskyy A

更新日期：2009-07-01 00:00:00

abstract：:The objectives of this work were to demonstrate the potential of a two-phase sequencing batch reactor in degrading xenobiotics and to evaluate the kinetic parameters leading to a mathematical model of the system. 4-Nitrophenol (4NP), a typical representative of substituted phenols, was selected as the target xenobioti...

journal_title：Applied microbiology and biotechnology

authors： Tomei MC,Annesini MC,Rita S,Daugulis AJ

更新日期：2008-10-01 00:00:00

abstract：:Isoprenoids, also referred to as terpenes, are the most diverse class of natural products appearing in a variety of natural sources, specifically in higher plants, and have a wide range of biological functions. This review describes novel or recent approaches and achievements in pathway engineering of Escherichia coli...

journal_title：Applied microbiology and biotechnology

authors： Harada H,Misawa N

更新日期：2009-10-01 00:00:00