A genetically modified solvent-tolerant bacterium for optimized production of a toxic fine chemical.

abstract：:Cost and energy reductions in the production process of bismuth chalcogenide (BC) semiconductor materials are essential to make thermoelectric generators comprised of BCs profitable and CO2 neutral over their life cycle. In this study, as an eco-friendly production method, bismuth selenide (Bi2Se3) nanoparticles were ...

journal_title：Applied microbiology and biotechnology

authors： Kuroda M,Suda S,Sato M,Ayano H,Ohishi Y,Nishikawa H,Soda S,Ike M

更新日期：2019-11-01 00:00:00

abstract：:Butenyl-spinosyn produced by Saccharopolyspora pogona exhibits strong insecticidal activity and broad pesticidal spectrum. However, its synthetic level was low in the wild-type strain. At present, important functional genes involved in butenyl-spinosyn biosynthesis remain unknown, which leads to difficulty in efficien...

journal_title：Applied microbiology and biotechnology

authors： Rang J,Zhu Z,Li Y,Cao L,He H,Tang J,Hu J,Chen J,Hu S,Huang W,Yu Z,Ding X,Sun Y,Xie Q,Xia L

更新日期：2021-01-23 00:00:00

abstract：:Biofilms are defined as microbial cell layers, which are irreversibly or reversibly attached on solid surfaces. These attached cells are embedded in a self-produced exopolysaccharide matrix, and exhibit different growth and bioactivity compared with suspended cells. With their high biomass density, stability, and pote...

journal_title：Applied microbiology and biotechnology

authors： Cheng KC,Demirci A,Catchmark JM

更新日期：2010-06-01 00:00:00

abstract：:Ammonia was observed as a potential significant factor to manipulate the abundance and activity of ammonia-oxidizing microorganisms (AOMs) in water environments. For the first time, this study confirmed this phenomenon by laboratory cultivation. In a series of estuarine sediment-coastal water microcosms, we investigat...

journal_title：Applied microbiology and biotechnology

authors： Zhang Y,Chen L,Dai T,Sun R,Wen D

更新日期：2015-08-01 00:00:00

abstract：:With their ability to lyse Gram-positive bacteria, phage lytic enzymes (or lysins) have received a great deal of attention as novel anti-infective agents. The number of known genes encoding these peptidoglycan hydrolases has increased markedly in recent years, due in large part to advances in DNA sequencing technology...

journal_title：Applied microbiology and biotechnology

authors： Schmitz JE,Ossiprandi MC,Rumah KR,Fischetti VA

更新日期：2011-03-01 00:00:00

abstract：INTRODUCTION:Hitherto the production of the biopolymer cyanophycin (CGP) using recombinant Escherichia coli strains and cheap mineral salts medium yielded only trace amounts of CGP (<0.5%, w/w) of the cell dry matter (CDM). This was probably due to the instability of the plasmids encoding the cyanophycin synthetase. M...

journal_title：Applied microbiology and biotechnology

authors： Kroll J,Klinter S,Steinbüchel A

更新日期：2011-02-01 00:00:00

abstract：:Humus as an electron mediator is recognized as an effective strategy to improve the biological transformation and degradation of toxic substances, yet the action of humus in microbial detoxification of chromate is still unknown. In this study, a humus-reducing strain 3C(3) was isolated from mangrove sediment. Based on...

journal_title：Applied microbiology and biotechnology

authors： Hong Y,Wu P,Li W,Gu J,Duan S

更新日期：2012-03-01 00:00:00

abstract：:The aim of this study was to elucidate the kinetic constraints during the redox biotransformation of the azo dye, Reactive Red 2 (RR2), and carbon tetrachloride (CT) mediated by soluble humic acids (HAs) and immobilized humic acids (HAi), as well as by the quinoid model compounds, anthraquinone-2,6-disulfonate (AQDS) ...

journal_title：Applied microbiology and biotechnology

authors： Cervantes FJ,Martínez CM,Gonzalez-Estrella J,Márquez A,Arriaga S

更新日期：2013-03-01 00:00:00

abstract：:An enzyme able to cleave the 3',5'-phosphate ring of 2'-methoxyethyl cyclic nucleotides (3',5'-cyclic nucleotide phosphodiesterase, EC 3.1.4.17) from Serratia marcescens DSM 30121 was used to deprotect the cyclic phosphate nucleotides after chemical alkylation. The process yielded 2'-O-alkylated nucleosides used as bu...

journal_title：Applied microbiology and biotechnology

authors： Marais G,Ghisalba O

更新日期：2005-02-01 00:00:00

abstract：:Production of (R)-3-hydroxybutyric acid [(R)-3-HB] by strain Halomonas sp. KM-1 under successive aeration conditions was investigated. The first aerobic condition allowed both cell growth and intracellular storage of poly-(R)-3-hydroxybutyric acid (PHB). The second microaerobic condition, achieved by reducing the cult...

journal_title：Applied microbiology and biotechnology

authors： Kawata Y,Kawasaki K,Shigeri Y

更新日期：2012-11-01 00:00:00

abstract：:DNA microarrays have found widespread use as a flexible tool to investigate bacterial metabolism. Their main advantage is the comprehensive data they produce on the transcriptional response of the whole genome to an environmental or genetic stimulus. This allows the microbiologist to monitor metabolism and to define s...

journal_title：Applied microbiology and biotechnology

authors： Ehrenreich A

更新日期：2006-11-01 00:00:00

abstract：:Conductive materials can facilitate syntrophic methane (CH4) production by improving direct interspecies electron transfer. The effect of a conductive material, ferroferric oxide (Fe3O4), on the anaerobic treatment of tryptone-based high-strength wastewater was investigated in two anaerobic sequencing batch reactors (...

journal_title：Applied microbiology and biotechnology

authors： Yin Q,He K,Liu A,Wu G

更新日期：2017-05-01 00:00:00

abstract：:Linkages among bioreactor operation and performance and microbial community structure were investigated for a fixed-bed biofilm system designed to remove perchlorate from drinking water. Perchlorate removal was monitored to evaluate reactor performance during and after the frequency and intensity of the backwash proce...

journal_title：Applied microbiology and biotechnology

authors： Li X,Yuen W,Morgenroth E,Raskin L

更新日期：2012-11-01 00:00:00

abstract：:In response to changing extracellular pH levels, phosphate-limited continuous cultures of Clostridium acetobutylicum reversibly switches its metabolism from the dominant formation of acids to the prevalent production of solvents. Previous experimental and theoretical studies have revealed that this pH-induced metaboli...

journal_title：Applied microbiology and biotechnology

authors： Millat T,Janssen H,Thorn GJ,King JR,Bahl H,Fischer RJ,Wolkenhauer O

更新日期：2013-07-01 00:00:00

abstract：:Corynebacterium glutamicum, a gram-positive soil bacterium, has been regarded as an aerobe because its growth by fermentative catabolism or by anaerobic respiration has, to this date, not been demonstrated. In this study, we report on the anaerobic growth of C. glutamicum in the presence of nitrate as a terminal elect...

journal_title：Applied microbiology and biotechnology

authors： Nishimura T,Vertès AA,Shinoda Y,Inui M,Yukawa H

更新日期：2007-06-01 00:00:00

abstract：:Atherosclerosis is a major cause of mortalities and morbidities worldwide. It is associated with hyperlipidemia and inflammation, and become chronic by triggering metabolites in different metabolic pathways. Disturbance in the human gut microbiota is now considered a critical factor in the atherosclerosis. Trimethylam...

journal_title：Applied microbiology and biotechnology

authors： Din AU,Hassan A,Zhu Y,Yin T,Gregersen H,Wang G

更新日期：2019-12-01 00:00:00

abstract：:Using organically synthesized hydroxyalkanoate coenzyme A thioesters, the activities of two short-chain polyhydroxalkanoate (PHA) synthases were investigated--Ralstonia eutropha PHA synthase (a type I PHA synthase) and Ectothiorhodospira shaposhnikovii PHA synthase (a type III synthase). The results indicate that the ...

journal_title：Applied microbiology and biotechnology

authors： Zhang S,Kamachi M,Takagi Y,Lenz RW,Goodwin S

更新日期：2001-07-01 00:00:00

abstract：:A Saccharomyces-cerevisiae-based simultaneous saccharification and fermentation (SSF) of lignocellulosic biomass is limited to an operating temperature of about 37 degrees C, and even a small increase in temperature can have a deleterious effect. This points to a need for a more thermotolerant yeast. To this end, S. c...

journal_title：Applied microbiology and biotechnology

authors： Kadam KL,Schmidt SL

更新日期：1997-12-01 00:00:00

abstract：:Short interfering RNA (siRNA) is one of the members of the family of RNA interference (RNAi). Coupled with the RNA-induced silencing complex (RISC), siRNA is able to trigger the cleavage of target RNAs which serve as a defensive system against pathogens. Meanwhile, siRNA in gene silencing opens a new avenue for the tr...

journal_title：Applied microbiology and biotechnology

authors： Tam C,Wong JH,Cheung RCF,Zuo T,Ng TB

更新日期：2017-10-01 00:00:00

abstract：:Novel alginate hydrogels with silver nanoparticles (AgNPs) and honey components were produced with the aim to target multidrug-resistant bacterial strains causing nosocomial wound infections. AgNP synthesis was optimized in highly concentrated honey solutions so that a 5-month stable, colloid solution with 50% of hone...

journal_title：Applied microbiology and biotechnology

authors： Stojkovska J,Petrovic P,Jancic I,Milenkovic MT,Obradovic B

更新日期：2019-10-01 00:00:00

abstract：:The response behavior of three dissimilatory perchlorate-reducing bacteria to different electron acceptors (nitrate, chlorate, and perchlorate) was investigated with two different assays. The observed response was species-specific, dependent on the prior growth conditions, and was inhibited by oxygen. We observed attr...

journal_title：Applied microbiology and biotechnology

authors： Sun Y,Gustavson RL,Ali N,Weber KA,Westphal LL,Coates JD

更新日期：2009-10-01 00:00:00

abstract：:We collected several biofilm samples from Japanese rivers and established a reproducible multi-species biofilm model that can be analyzed in laboratories. Bacterial abundance at the generic level was highly similar between the planktonic and biofilm communities, whereas comparative metatranscriptomic analysis revealed...

journal_title：Applied microbiology and biotechnology

authors： Nakamura Y,Yamamoto N,Kino Y,Yamamoto N,Kamei S,Mori H,Kurokawa K,Nakashima N

更新日期：2016-08-01 00:00:00

abstract：:Extracellular polymeric substance (EPS) is proposed to facilitate calcium ion supersaturation through its nucleation effect during the microbially induced calcium carbonate precipitation (MICP) process. However, the supersaturation effect of Ca2+ via EPS in MICP has not been clearly demonstrated. Enhanced exopolysacch...

journal_title：Applied microbiology and biotechnology

authors： Kim HJ,Shin B,Lee YS,Park W

更新日期：2017-08-01 00:00:00

abstract：:Microtiter plates were used as minireactors to study Starmerella bombicola growth and sophorolipid (SL) production. Compositional analysis of SL mixtures by liquid chromatography with electrospray ionization tandem mass spectrometry showed similar results on SLs produced using the laboratory scale (shake flask) and th...

journal_title：Applied microbiology and biotechnology

authors： Ribeiro IA,Bronze MR,Castro MF,Ribeiro MH

更新日期：2013-03-01 00:00:00

abstract：:Bacillus thuringiensis bacteria show insecticidal activities that rely upon the production of insecticidal crystal proteins, which are encoded by cry or cyt genes and can target a variety of insect pests. It has been shown that cry1Ac is the only cry gene in B. thuringiensis subsp. kurstaki HD73 (B. thuringiensis HD73...

journal_title：Applied microbiology and biotechnology

authors： Zhang X,Gao T,Peng Q,Song L,Zhang J,Chai Y,Sun D,Song F

更新日期：2018-04-01 00:00:00

abstract：:This study examines the bioremediation potential and cadmium-induced cellular response on a molecular level in Candida tropicalis 3Aer. Spectroscopic analysis clearly illustrated the involvement of yeast cell wall components in biosorption. Cadmium bioaccumulation was confirmed by TEM, SEM, and EDX examination. TEM im...

journal_title：Applied microbiology and biotechnology

authors： Khan Z,Rehman A,Nisar MA,Zafar S,Hussain SZ,Zerr I,Hussain I,Waseem M,Arif M

更新日期：2017-10-01 00:00:00

abstract：:This paper reports the in vivo expression of the synthetic transposase gene tnp(a) from a hyperactive Tn5 tnp gene mutant in Streptomyces coelicolor. Using the synthetic tnp(a) gene adapted for Streptomyces codon usage, we showed random insertion of the transposon into the Streptomycetes genome. The insertion frequenc...

journal_title：Applied microbiology and biotechnology

authors： Petzke L,Luzhetskyy A

更新日期：2009-07-01 00:00:00

abstract：:Aromatic amino acid metabolism in yeast is an important source of secondary compounds that influence the aroma and flavour of alcoholic beverages and foods. Examples are the higher alcohol 2-phenylethanol, and its acetate ester, 2-phenylethyl acetate, which impart desirable floral aromas in wine, beer and baker's prod...

journal_title：Applied microbiology and biotechnology

authors： Cordente AG,Schmidt S,Beltran G,Torija MJ,Curtin CD

更新日期：2019-06-01 00:00:00

abstract：:Cupriavidus necator JMP134 utilizes meta-nitrophenol (MNP) as a sole source of carbon, nitrogen, and energy. The metabolic reconstruction of MNP degradation performed in silico suggested that the mnp cluster might have played important roles in MNP degradation. In order to experimentally confirm the prediction, we hav...

journal_title：Applied microbiology and biotechnology

authors： Yin Y,Xiao Y,Liu HZ,Hao F,Rayner S,Tang H,Zhou NY

更新日期：2010-08-01 00:00:00

abstract：:Bacteria from the genus Geobacillus are generally obligately thermophilic, with a unique bioenergy production capacity and unique enzymes. Geobacillus species were isolated primarily from hot springs, oilfields, and associated soils. They often exhibit unique survival patterns in these extreme oligotrophic environment...

journal_title：Applied microbiology and biotechnology

authors： Lin JH,Zhang KC,Tao WY,Wang D,Li S

更新日期：2019-10-01 00:00:00