Novel approaches and achievements in biosynthesis of functional isoprenoids in Escherichia coli.

abstract：:In the course of more than 60-year history, penicillin G acylase (PGA) gained a unique position among enzymes used by pharmaceutical industry for production of β-lactam antibiotics. Kinetically controlled enzymatic syntheses of cephalosporins of novel generations in which PGA catalyzes coupling of activated acyl donor...

journal_title：Applied microbiology and biotechnology

authors： Marešová H,Plačková M,Grulich M,Kyslík P

更新日期：2014-04-01 00:00:00

abstract：:Cheese whey (CW), the liquid resulting from the precipitation and removal of milk casein during cheese-making, and the second cheese whey (SCW) derived from the production of cottage and ricotta cheeses are the main byproducts of dairy industry. The major constituent of CW and SCW is lactose, contributing to the high ...

journal_title：Applied microbiology and biotechnology

authors： Zotta T,Solieri L,Iacumin L,Picozzi C,Gullo M

更新日期：2020-04-01 00:00:00

abstract：:Previously, we reported that the expression of Bombyx mori 30Kc6 gene in Chinese hamster ovary (CHO) cells increases recombinant protein production by both inhibiting apoptosis and enhancing specific productivity. In this study, in order to gain a thorough understanding of the roles of 30Kc6 gene in antibody productio...

journal_title：Applied microbiology and biotechnology

authors： Wang Z,Ma X,Fan L,Rhee WJ,Park TH,Zhao L,Tan WS

更新日期：2012-06-01 00:00:00

abstract：:Overuse of broad-spectrum antibiotics to control human and plant pathogens greatly accelerated the development of antibiotic resistance among bacteria and fungi. Therefore, usage of new approaches is necessary to control outbreaks of phytopathogenic diseases as well as multidrug-resistant human pathogens. Many of the ...

journal_title：Applied microbiology and biotechnology

authors： Olishevska S,Nickzad A,Déziel E

更新日期：2019-02-01 00:00:00

abstract：:Physiological heterogeneity constitutes a critical parameter in biotechnological systems since both metabolite yield and productivity are often hampered by the presence of undesired physiological cell subpopulations. In the present study, the physiological status and functionality of Pseudomonas taetrolens cells were ...

journal_title：Applied microbiology and biotechnology

authors： Alonso S,Rendueles M,Díaz M

更新日期：2012-12-01 00:00:00

abstract：:The marine organism Moritella marina MP-1 produces the polyunsaturated fatty acid docosahexaenoic acid (DHA). While the basic metabolic pathway for DHA production in this organism has been identified, the impact of growth conditions on DHA production is largely unknown. This study examines the effect of supplemental c...

journal_title：Applied microbiology and biotechnology

authors： Kautharapu KB,Rathmacher J,Jarboe LR

更新日期：2013-04-01 00:00:00

abstract：:The microbial production of biofuels is a promising avenue for the development of viable processes for the generation of fuels from sustainable resources. In order to become cost and energy effective, these processes must utilize organisms that can be optimized to efficiently produce candidate fuels from a variety of ...

journal_title：Applied microbiology and biotechnology

authors： Clomburg JM,Gonzalez R

更新日期：2010-03-01 00:00:00

abstract：:Ralstonia eutropha H850 was labelled chromosomally with a gfp marker gene encoding for the green fluorescent protein, and designated R. eutropha H850g13. Visual observation of green fluorescent cells under an epifluorescence microscope, and PCR amplification products, confirmed that the bacterium was labelled with gfp...

journal_title：Applied microbiology and biotechnology

authors： Abbey AM,Beaudette LA,Lee H,Trevors JT

更新日期：2003-12-01 00:00:00

abstract：:Aspergillus niger phytase (PhyA) has been used as a feed supplement to reduce manure phosphorus excretion of swine and poultry but lacks sufficient thermostability for feed pelleting and appropriate pH-activity profile for phytate hydrolysis in the stomach of animals. Previously, a thermostable mutant PhyA18 and two p...

journal_title：Applied microbiology and biotechnology

authors： Zhang W,Lei XG

更新日期：2008-01-01 00:00:00

abstract：:Vibrio parahaemolyticus is one of the major pathogens responsible for vibriosis and zoonotic infections in teleosts, marine invertebrates, and also humans through consumption of contaminated or unprocessed seafood. Emergence of resistance against current accessible antibiotics and spread to the food chain and environm...

journal_title：Applied microbiology and biotechnology

authors： Karan S,Choudhury D,Dixit A

更新日期：2021-01-01 00:00:00

abstract：:Eight Escherichia coli strains were studied in minimal medium with a continuous flow system using confocal microscopy. K12 wild-type strains ATCC 25404 and MG1655 formed the best biofilms ( approximately 43 microm thick, 21 to 34% surface coverage). JM109, DH5alpha, and MG1655 motA formed intermediate biofilms ( appro...

journal_title：Applied microbiology and biotechnology

authors： Wood TK,González Barrios AF,Herzberg M,Lee J

更新日期：2006-09-01 00:00:00

abstract：:The 5'-untranslated leader sequence (UTLS) of the slpA gene from Lactobacillus acidophilus contributes to mRNA stabilization by producing a 5' stem and loop structure, and a high-level expression system for the lactic acid bacteria was developed using the UTLS in this study. A plasmid, which expresses alpha-amylase un...

journal_title：Applied microbiology and biotechnology

authors： Narita J,Ishida S,Okano K,Kimura S,Fukuda H,Kondo A

更新日期：2006-11-01 00:00:00

abstract：:Coxsackievirus A16 (CA16) is one of the major causative agents of hand, foot, and mouth disease (HFMD). No CA16 vaccine candidates have progressed to clinical trials so far. Immunogenicity studies indicated that different CA16 particles have much influence on the efficacy of a candidate vaccine. However, there are sti...

journal_title：Applied microbiology and biotechnology

authors： Ye X,Yang L,Jia J,Han J,Li S,Liu Y,Xu L,Zhao H,Chen Y,Li Y,Cheng T,Xia N

更新日期：2016-03-01 00:00:00

abstract：:Suspension cultures of the endemic South-African plant Cyclopia subternata were established for the first time and evaluated for the presence of isoflavones. The influence of light, as well as medium supplementation strategies with phenylalanine, casein hydrolysate and coconut water on biomass growth and isoflavone pr...

journal_title：Applied microbiology and biotechnology

authors： Kokotkiewicz A,Luczkiewicz M,Kowalski W,Badura A,Piekus N,Bucinski A

更新日期：2013-10-01 00:00:00

abstract：:Site-saturation mutagenesis (SSM) has been used in directed evolution of proteins for a long time. As a special form of saturation mutagenesis, it involves individual randomization at a given residue with formation of all 19 amino acids. To date, the most efficient embodiment of SSM is a one-step PCR-based approach us...

journal_title：Applied microbiology and biotechnology

authors： Li A,Acevedo-Rocha CG,Reetz MT

更新日期：2018-07-01 00:00:00

abstract：:The natural genetic code only allows for 20 standard amino acids in protein translation, but genetic code reprogramming enables the incorporation of non-standard amino acids (NSAAs). Proteins containing NSAAs provide enhanced or novel properties and open diverse applications. With increased attention to the recent adv...

journal_title：Applied microbiology and biotechnology

authors： Jin X,Park OJ,Hong SH

更新日期：2019-04-01 00:00:00

abstract：:Escherichia coli and Lactobacillus plantarum were subjected to final water potentials of -5.6 MPa and -11.5 MPa with three solutes: glycerol, sorbitol and NaCl. The water potential decrease was realized either rapidly (osmotic shock) or slowly (20 min) and a difference in cell viability between these conditions was on...

journal_title：Applied microbiology and biotechnology

authors： Poirier I,Maréchal PA,Evrard C,Gervais P

更新日期：1998-12-01 00:00:00

abstract：:The complete biodegradation of azo dye, Fast Acid Red GR, was observed under microaerophilic conditions by Shewanella decolorationis S12. Although the highest decolorizing rate was measured under anaerobic condition and the highest biomass was obtained under aerobic condition, a further biodegradation of decolorizing ...

journal_title：Applied microbiology and biotechnology

authors： Xu M,Guo J,Sun G

更新日期：2007-09-01 00:00:00

abstract：:Conventional acetone-butanol-ethanol (ABE) fermentation coupled with gas stripping is conducted under strict anaerobic conditions. In this work, a fed-batch ABE fermentation integrated with gas stripping (FAFIGS) system using a non-strict anaerobic butanol-producing symbiotic system, TSH06, was investigated for the ef...

journal_title：Applied microbiology and biotechnology

authors： Lin Z,Liu H,Yan X,Zhou Y,Cheng K,Zhang J

更新日期：2017-11-01 00:00:00

abstract：:Lactobacillus species are attractive hosts for the expression of heterologous proteins, antigens, vaccines, and drugs due to their GRAS (generally recognized as safe) status. The bioengineering techniques open new possibilities of improving Lactobacillus strains. In this regard, the control of the gene expression in L...

journal_title：Applied microbiology and biotechnology

authors： Peirotén Á,Landete JM

更新日期：2020-05-01 00:00:00

abstract：:This study was aimed to investigate the effect of a single-chain fragment variable antibody of connective tissue growth factor (anti-CTGF scFv) against the differentiation of fibroblast into myofibroblast. The scFv antibody was firstly expressed in Escherichia coli cells and was then purified by affinity chromatograph...

journal_title：Applied microbiology and biotechnology

authors： Wu G,Wang X,Deng X,Wu P,Xue X,Yan X,Wang X

更新日期：2012-03-01 00:00:00

abstract：:2-Oxoglutarate (2OG) is a metabolite from the highly conserved Krebs cycle and not only plays a critical role in metabolism but also acts as a signaling molecule in a variety of organisms. Environmental inorganic nitrogen is reduced to ammonium by microorganisms, whose metabolic pathways involve the conversion of 2OG ...

journal_title：Applied microbiology and biotechnology

authors： Zhang C,Wei ZH,Ye BC

更新日期：2013-09-01 00:00:00

abstract：:Epitope vaccine based on the enzyme urease of Helicobacter pylori is a promising option for prophylactic and therapeutic vaccination against H. pylori infection. In our previous study, the epitope vaccine CTB-UA, which was composed of the mucosal adjuvant cholera toxin B subunit (CTB) and an epitope (UreA₁₈₃₋₂₀₃) from...

journal_title：Applied microbiology and biotechnology

authors： Guo L,Liu K,Xu G,Li X,Tu J,Tang F,Xing Y,Xi T

更新日期：2012-09-01 00:00:00

abstract：:Purines are a primary source of carbon and nitrogen in soil; however, their metabolism is poorly understood in Streptomyces. Using a combination of proteomics, metabolomics, and metabolic engineering, we characterized the allantoin pathway in Streptomyces coelicolor. When cells grew in glucose minimal medium with alla...

journal_title：Applied microbiology and biotechnology

authors： Navone L,Casati P,Licona-Cassani C,Marcellin E,Nielsen LK,Rodriguez E,Gramajo H

更新日期：2014-01-01 00:00:00

abstract：:The existence of taste and odour (T&O) in drinking water is one of the principal causes of consumer complaints and is commonly related to algae growth. Numerous studies have confirmed the existence of algal blooms emerging specifically in low-temperature periods, herein referred to as "cold algae"; these include chrys...

journal_title：Applied microbiology and biotechnology

authors： Cen C,Zhang K,Zhang T,Zhou X,Pan R

更新日期：2020-11-01 00:00:00

abstract：:MoHrip2, identified from Magnaporthe oryzae as an elicitor, can activate plant defense responses either in the form of recombinant protein in vitro or ectopic expressed protein in rice. However, its intrinsic function in the infective interaction of M. oryzae-rice is largely unknown. Here, we found that mohrip2 expres...

journal_title：Applied microbiology and biotechnology

authors： Nie H,Zhang L,Zhuang H,Yang X,Qiu D,Zeng H

更新日期：2019-08-01 00:00:00

abstract：:Application of soil amendments has been wildly used to increase soil pH and control bacterial wilt. However, little is known about causal shifts in the rhizosphere microbial community of crops, especially when the field naturally harbors the disease of bacterial wilt to tobacco for many years due to long-term continuo...

journal_title：Applied microbiology and biotechnology

authors： Shen G,Zhang S,Liu X,Jiang Q,Ding W

更新日期：2018-11-01 00:00:00

abstract：:Three different mechanisms can be envisaged that are used by fungi to solubilize coal: the production of alkaline substances, the extrusion of chelators and, of special interest in the scope of biotechnology, the action of enzymes. Whether these mechanisms are operating separately or in various combinations has not ye...

journal_title：Applied microbiology and biotechnology

authors： Holker U,Ludwig S,Scheel T,Hofer M

更新日期：1999-07-01 00:00:00

abstract：:Shikimic acid is an industrially important chiral compound used as a key ingredient in formulation of drug Oseltamivir phosphate (Tamiflu) for the treatment of swine/avian flu. The high cost and limited availability of shikimic acid isolated from plants has detained the use of this valuable building block of the drug....

journal_title：Applied microbiology and biotechnology

authors： Rawat G,Tripathi P,Saxena RK

更新日期：2013-05-01 00:00:00

abstract：:The effects of methyl jasmonate (MJ) elicitation on the cell growth and accumulation of ginsenoside in 5-l bioreactor suspension cultures of Panax ginseng were investigated. Ginsenoside accumulation was enhanced by elicitation by MJ (in the range 50-400 microM); however, fresh weight, dry weight and growth ratio of th...

journal_title：Applied microbiology and biotechnology

authors： Thanh NT,Murthy HN,Yu KW,Hahn EJ,Paek KY

更新日期：2005-04-01 00:00:00