Abstract：
:In the course of more than 60-year history, penicillin G acylase (PGA) gained a unique position among enzymes used by pharmaceutical industry for production of β-lactam antibiotics. Kinetically controlled enzymatic syntheses of cephalosporins of novel generations in which PGA catalyzes coupling of activated acyl donor with nucleophile belong among the latest large-scale applications. Contrary to rather specific roles of other enzymes involved in β-lactam biocatalyses, the PGA seems to have the greatest potential. On the laboratory scale, other applications with industrial potential were described, e.g., directed evolution of the enzyme to meet specific demands of industrial processes or its modification into the enzyme catalyzing reactions with novel substrates. The fact that β-lactams represent the most important group of antibiotics comprising 65 % of the world antibiotic market explains such a tremendous and continuous interest in this enzyme. Indeed, the annual consumption of PGA has recently been estimated to range from 10 to 30 million tons. The application potential of the enzyme goes beyond the β-lactam biocatalysis due to its enantioselectivity and promiscuity: the PGA can be used for the production of achiral and chiral compounds convenient for the preparation of synthons and active pharmaceutical ingrediences, respectively. These biocatalyses, however, still wait for large-scale application.
journal_name
Appl Microbiol Biotechnoljournal_title
Applied microbiology and biotechnologyauthors
Marešová H,Plačková M,Grulich M,Kyslík Pdoi
10.1007/s00253-013-5492-7subject
Has Abstractpub_date
2014-04-01 00:00:00pages
2867-79issue
7eissn
0175-7598issn
1432-0614journal_volume
98pub_type
杂志文章,评审abstract：:This paper reports a novel integrated metabolic pathway of amino acid production through the utilization of phenol in Corynebacterium glutamicum. In the presence of 8.5 mM phenol, the level of glutamate and proline production increased up to 1.2- and 14.7-fold, respectively, compared to the control condition. In addit...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-009-2206-2
更新日期：2010-01-01 00:00:00
abstract：:Problems with deflocculation and solids separation in biological wastewater treatment systems are linked to fluctuations in physicochemical conditions. This study examined the composition of activated sludge bacterial communities in lab-scale sequencing batch reactors treating bleached kraft mill effluent, under trans...
journal_title：Applied microbiology and biotechnology
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更新日期：2010-11-01 00:00:00
abstract：:Although the niche differentiation of anammox bacteria has been extensively observed in a lab-scale reactor, the inherent mechanism for this ecological phenomenon is still elusive. Here, we combined the long-term reactor operation, genome-centered metagenome, and metatranscriptome analyses to gain insight into the sub...
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abstract：:Resolution of (R,S)-ibuprofen enantiomers by esterification in different organic solvents was studied using Candida cylindracea lipase. This enzyme preparation had high enantiospecificity for S(+)-ibuprofen in the esterification reaction of a racemic ibuprofen with primary alcohols. The esterification yields of second...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/BF00169420
更新日期：1992-10-01 00:00:00
abstract：:Nitrilase-mediated hydrolysis of isobutylsuccinonitrile (IBSN) is a highly attractive approach for (S)-3-cyano-5-methylhexanoic acid ((S)-CMHA), the critical chiral intermediate of pregabalin. In this study, a robust nitrilase from Arabis alpina (AaNIT) was screened and engineered. The N258D mutant was obtained with h...
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pub_type： 杂志文章
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更新日期：2020-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s00253-019-09744-9
更新日期：2019-04-01 00:00:00
abstract：:Enzymatic kinetic resolution is a widely used biotechnological tool for the production of enantiomerically pure/enriched compounds. This technique takes advantage of the enantioselectivity or enantiospecificity of an enzyme for one of the enantiomers of a racemic substrate to isolate the desired isomer. N-Carbamoyl-D-...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章,评审
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更新日期：2010-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：2016-08-01 00:00:00
abstract：:Hydroxy fatty acids (HFA) have gained importance because of their special properties such as higher viscosity and reactivity compared with other non-hydroxy fatty acids. The bacterial isolate Pseudomonas aeruginosa (PR3) was reported to produce mono-, di-, and trihydroxy fatty acids from different unsaturated fatty ac...
journal_title：Applied microbiology and biotechnology
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doi：10.1007/s00253-006-0662-5
更新日期：2007-02-01 00:00:00
abstract：:Mediated electrochemical detection of catabolism in prokaryotic cells is well documented; however, the application of this technique to eukaryotic cells has received less attention. Two catabolic substrate-dependent mediated electrochemical signals were detected in the yeast Saccharomyces cerevisiae. The signal using ...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-002-1108-3
更新日期：2002-10-01 00:00:00
abstract：:Terminal disinfection and daily cleaning have been performed in hospitals in Taiwan for many years to reduce the risks of healthcare-associated infections. However, the effectiveness of these cleaning approaches and dynamic changes of surface microbiota upon cleaning remain unclear. Here, we report the surface changes...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-016-7846-4
更新日期：2017-01-01 00:00:00
abstract：:Biotechnological production of vanillin is gaining momentum as the natural synthesis of vanillin that is very expensive. Ferulic acid (FA), a costly compound, is used as the substrate to produce vanillin biotechnologically and the making process is still expensive. Therefore, this study investigated the practical use ...
journal_title：Applied microbiology and biotechnology
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doi：10.1007/s00253-017-8283-8
更新日期：2017-07-01 00:00:00
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journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-012-4379-3
更新日期：2013-02-01 00:00:00
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journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章
doi：10.1007/s00253-015-6686-y
更新日期：2015-11-01 00:00:00
abstract：:The worldwide interest of the current era is to increase tendency towards the use of natural substances instead of synthetic ones. So, alternative and effective environment friendly sustainable technologies are highly needed. Due to a broad range of biological activities, fungi are considered as a significant source o...
journal_title：Applied microbiology and biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s00253-015-7231-8
更新日期：2016-02-01 00:00:00
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journal_title：Applied microbiology and biotechnology
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doi：10.1007/s00253-015-6416-5
更新日期：2015-07-01 00:00:00
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更新日期：2011-04-01 00:00:00
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journal_title：Applied microbiology and biotechnology
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journal_title：Applied microbiology and biotechnology
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更新日期：1998-09-01 00:00:00
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journal_title：Applied microbiology and biotechnology
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更新日期：1997-04-01 00:00:00
abstract：:Streptomyces is an interesting host for the secretory production of recombinant proteins because of its innate capacity to secrete proteins at high level in the culture medium. In this report, we evaluated the importance of the phage-shock protein A (PspA) homologue on the protein secretion yield in Streptomyces livid...
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更新日期：2007-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s00253-018-9216-x
更新日期：2018-09-01 00:00:00
abstract：:The molecular mechanisms active during the endophytic phase of the fungus Pochonia chlamydosporia are still poorly understood. In particular, few data are available on the links between the endophyte and the root response, as modulated by noncoding small RNAs. In this study, we describe the microRNAs (miRNAs) that are...
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更新日期：2018-01-01 00:00:00
