pspA overexpression in Streptomyces lividans improves both Sec- and Tat-dependent protein secretion.

abstract：:The obligate photoautotrophic cyanobacterium Synechococcus PCC7942 and the photoheterotrophic heterocystous cyanobacterium Noctoc muscorum are able to reduce prochiral ketones asymmetrically to optical pure chiral alcohols without light. An example is the synthesis of S-pentafluoro(phenyl-)ethanol with an enantiomeric...

journal_title：Applied microbiology and biotechnology

authors： Havel J,Weuster-Botz D

更新日期：2007-07-01 00:00:00

abstract：:The cell envelope-associated proteinase (CEP) of Lactobacillus delbrueckii subsp. lactis CRL 581 (PrtL) has an essential role in bacterial growth, contributes to the flavor and texture development of fermented products, and can release bioactive health-beneficial peptides during milk fermentation. The genome of L. del...

journal_title：Applied microbiology and biotechnology

authors： Villegas JM,Brown L,Savoy de Giori G,Hebert EM

更新日期：2015-05-01 00:00:00

abstract：:The effects of copper (Cu), chromium (Cr), cadmium (Cd), lead (Pb) and zinc (Zn) on the biotransformation of organic acids (acetate, propionate and butyrate) and H2 were assessed in serum-bottle microcosms. Experiments were performed over a range of metal concentrations (20-200 mg/l) using biomass from an anaerobic bi...

journal_title：Applied microbiology and biotechnology

authors： Kong IC,Hubbard JS,Jones WJ

更新日期：1994-11-01 00:00:00

abstract：:Cultivation of Aspergillus terreus ATCC 20542 in a stirred tank bioreactor was performed to induce the biosynthesis of secondary metabolites and provide the bioprocess-related insights into the metabolic capabilities of the investigated strain. The activation of biosynthetic routes was attempted by the diversification...

journal_title：Applied microbiology and biotechnology

authors： Boruta T,Bizukojc M

更新日期：2016-04-01 00:00:00

abstract：:Polyunsaturated fatty acids (PUFAs) are essential lipids for cell function, normal growth, and development, serving as key structural components of biological membranes and modulating critical signal transduction events. Omega-3 (n-3) long chain PUFAs (LC-PUFAs) such as eicosapentaenoic acid (EPA) and docosahexaenoic ...

journal_title：Applied microbiology and biotechnology

authors： Wang M,Chen H,Ailati A,Chen W,Chilton FH,Todd Lowther W,Chen YQ

更新日期：2018-01-01 00:00:00

abstract：:Salicylate is an important intermediate in the bacterial degradation of polycyclic aromatic hydrocarbons and salicylate hydroxylases play essential roles in linking the peripheral and ring-cleavage catabolic pathways. Unlike the well-characterized salicylate 1-hydroxylases, the rarely occurred salicylate 5-hydroxylase...

journal_title：Applied microbiology and biotechnology

authors： Fang T,Zhou NY

更新日期：2014-01-01 00:00:00

abstract：:D-Xylonic acid is a versatile platform chemical with reported applications as complexing agent or chelator, in dispersal of concrete, and as a precursor for compounds such as co-polyamides, polyesters, hydrogels and 1,2,4-butanetriol. With increasing glucose prices, D-xylonic acid may provide a cheap, non-food derived...

journal_title：Applied microbiology and biotechnology

authors： Toivari MH,Nygård Y,Penttilä M,Ruohonen L,Wiebe MG

更新日期：2012-10-01 00:00:00

abstract：:Poly(hydroxyalkanoates) (PHAs) constitute biodegradable polyesters and are considered among the most promising candidates to replace common petrochemical plastics in various applications. To date, all commercial processes for PHA production employ microbial discontinuous fed-batch fermentations. These processes featur...

journal_title：Applied microbiology and biotechnology

authors： Atlić A,Koller M,Scherzer D,Kutschera C,Grillo-Fernandes E,Horvat P,Chiellini E,Braunegg G

更新日期：2011-07-01 00:00:00

abstract：:The ability of taxonomically different yeasts to synthesize pyruvic acid (PA) from glucose was studied. The study showed that many yeasts are able to produce PA from glucose under the condition of growth limitation by thiamine. This ability was found in the yeast Blastobotrys adeninivorans for the first time. The prod...

journal_title：Applied microbiology and biotechnology

authors： Kamzolova SV,Morgunov IG

更新日期：2016-09-01 00:00:00

abstract：:Saccharomyces cerevisiae is the organism of choice for many food and beverage fermentations because it thrives in high-sugar and high-ethanol conditions. However, the conditions encountered in bioethanol fermentation pose specific challenges, including extremely high sugar and ethanol concentrations, high temperature,...

journal_title：Applied microbiology and biotechnology

authors： Mukherjee V,Steensels J,Lievens B,Van de Voorde I,Verplaetse A,Aerts G,Willems KA,Thevelein JM,Verstrepen KJ,Ruyters S

更新日期：2014-11-01 00:00:00

abstract：:α-L-Rhamnosyl-β-D-glucosidase (rutinosidase) hydrolyzes the glycosidic linkage between the disaccharide 6-O-α-L-rhamnosyl-β-D-glucoside (rutinose) and the aglycone. We identified a hypothetical protein (annotated as AO090012000917) encoded in the Aspergillus oryzae genome that exhibits sequence similarity with Aspergi...

journal_title：Applied microbiology and biotechnology

authors： Ishikawa M,Kawasaki M,Shiono Y,Koseki T

更新日期：2018-04-01 00:00:00

abstract：:Hyaluronic acid (HA) is a ubiquitous polysaccharide found in humans, animals, bacteria, algae and molluscs. Simple yet sophisticated, HA demonstrates unique and valuable rheological properties. In solution, HA behaves as a stiffened random coil and the resultant behaviour, even at low concentrations, is far from Newto...

journal_title：Applied microbiology and biotechnology

authors： Marcellin E,Steen JA,Nielsen LK

更新日期：2014-08-01 00:00:00

abstract：:5-Hydroxytryptophan (5-HTP) is the precursor of the neurotransmitter serotonin and has been used for the treatment of various diseases such as depression, insomnia, chronic headaches, and binge eating associated obesity. The production of 5-HTP had been achieved in our previous report, by the development of a recombin...

journal_title：Applied microbiology and biotechnology

authors： Xu D,Fang M,Wang H,Huang L,Xu Q,Xu Z

更新日期：2020-03-01 00:00:00

abstract：:The osmotic tolerance of cells of Saccharomyces cerevisiae as a function of glycerol concentration and temperature has been investigated. Results show that under isothermal conditions (25 degrees C) cells are resistant (94% viability) to hyperosmotic treatment at 49.2 MPa. A thigher osmotic pressure, cell viability de...

journal_title：Applied microbiology and biotechnology

authors： Beney L,Marechal PA,Gervais P

更新日期：2001-08-01 00:00:00

abstract：:Synthetic biology aims at manipulating biological systems by rationally designed and genetically introduced components. Efforts in photoactuator engineering resulted in microorganisms reacting to extracellular light-cues with various cellular responses. Some of them lead to the formation of macroscopically observable ...

journal_title：Applied microbiology and biotechnology

authors： Renicke C,Taxis C

更新日期：2016-04-01 00:00:00

abstract：:Staphylococcus aureus causes severe infections and among all methicillin-resistant S. aureus (MRSA) remains a great challenge in spite of decade research of antibacterial compounds. Even though some synthetic antibiotics have been developed, they are not effective against MRSA, and hence, there is a search for natural...

journal_title：Applied microbiology and biotechnology

authors： Sinsinwar S,Vadivel V

更新日期：2020-10-01 00:00:00

abstract：:Escherichia coli normally cannot utilize the β-glucoside sugar cellobiose as a carbon and energy source unless a stringent selection pressure for survival is present. The cellobiose-utilization phenotype can be conferred by mutations in the two cryptic operons, chb and asc. In this study, the cellobiose-utilization ph...

journal_title：Applied microbiology and biotechnology

authors： Vinuselvi P,Lee SK

更新日期：2011-10-01 00:00:00

abstract：:Pseudomonas chlororaphis GP72 is a root-colonizing biocontrol strain isolated from the green pepper rhizosphere that synthesizes two phenazine derivatives: phenazine-1-carboxylic acid (PCA) and 2-hydroxyphenazine (2-OH-PHZ). The 2-OH-PHZ derivative shows somewhat stronger broad-spectrum antifungal activity than PCA, b...

journal_title：Applied microbiology and biotechnology

authors： Huang L,Chen MM,Wang W,Hu HB,Peng HS,Xu YQ,Zhang XH

更新日期：2011-01-01 00:00:00

abstract：:Benzene dioxygenase (BDO; EC 1.14.12.3) from Pseudomonas putida ML2 dihydroxylates benzene to produce cis-1,2-dihydroxy-cyclohexa-3,5-diene. As well as oxidising benzene and toluene, cell-free extracts of Escherichia coli JM109 expressing recombinant BDO oxidised cyclohexene, 1-methylcyclohexene and 3-methylcyclohexen...

journal_title：Applied microbiology and biotechnology

authors： Swift RJ,Carter SF,Widdowson DA,Mason JR,Leak DJ

更新日期：2001-06-01 00:00:00

abstract：:Low-energy ions exist widely in the natural world. People had neglected the interaction between low-energy ions and material; it was even more out of the question to study the relation of low-energy ions and the complicated organism until the biological effects of low-energy ion implantation were discovered in 1989. N...

journal_title：Applied microbiology and biotechnology

authors： Gu SB,Li SC,Feng HY,Wu Y,Yu ZL

更新日期：2008-02-01 00:00:00

abstract：:Confocal Raman microspectral imaging (CRMI) is an advanced cell-imaging method that maps endogenous molecular compositions with their unique spectral fingerprint indicators. The aim of this work was to provide a visualized understanding of subcellular features of live osteosarcoma cells using a 532-nm laser excitation...

journal_title：Applied microbiology and biotechnology

authors： Li J,Qin J,Zhang X,Wang R,Liang Z,He Q,Wang Z,Wang K,Wang S

更新日期：2019-08-01 00:00:00

abstract：:Due to efficient photosynthetic capability, robust growth, and clear genetic background, cyanobacteria are recently used for production of different biofuel and biochemical molecules by genetic engineering and showed great potentials as the next-generation microbial cell factory. For improving the production of bio-pr...

journal_title：Applied microbiology and biotechnology

authors： Tan X,Liang F,Cai K,Lu X

更新日期：2013-07-01 00:00:00

abstract：:Reduction and biodegradation mechanisms of naphthylaminesulfonic azo dye amaranth using a newly isolated Shewanella decolorationis strain S12 were investigated. Under anaerobic conditions, amaranth was reduced by strain S12, and a stoichiometric amount of two reduction products RP-1 and RP-2 were generated. UV/visible...

journal_title：Applied microbiology and biotechnology

authors： Hong Y,Guo J,Xu Z,Mo C,Xu M,Sun G

更新日期：2007-06-01 00:00:00

abstract：:In oyster ponds, the marine diatom Haslea ostrearia synthesises and excretes a hydrosoluble pigment of commercial interest called marennin. During the benthic stage, when algal cells are naturally immobilised in their own polysaccharides, marennin production is higher. To optimise this production, axenic cultures of H...

journal_title：Applied microbiology and biotechnology

authors： Lebeau T,Gaudin P,Moan R,Robert JM

更新日期：2002-07-01 00:00:00

abstract：:The effects of pH control strategy and fermentative operation modes on the biosynthesis of pyrroloquinoline quinine (PQQ) were investigated systematically with Methylobacillus sp. CCTCC M2016079 in the present work. Firstly, the shake-flask cultivations and benchtop fermentations at various pH values ranging from 5.3 ...

journal_title：Applied microbiology and biotechnology

authors： Si Z,Machaku D,Wei P,Huang L,Cai J,Xu Z

更新日期：2017-06-01 00:00:00

abstract：:We have characterized a mutant of Streptomyces clavuligerus NRRL 3585 which is almost completely blocked in cephalosporin biosynthesis and exhibits depressed activities of both the delta(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV) synthetase and cyclase enzymes of the cephalosporin pathway. A wild-type DNA region ...

journal_title：Applied microbiology and biotechnology

authors： Piret J,Resendiz B,Mahro B,Zhang JY,Serpe E,Romero J,Connors N,Demain AL

更新日期：1990-02-01 00:00:00

abstract：:The synergistic effect between polyoxometalates (POMs), namely K(5)[SiW(11)V(V)O(40)]·11H(2)O and H(5)[PMo(10)V(V) (2)O(40)]·13H(2)O and laccase from ascomycete Myceliophthora thermophila has been employed for the first time in oxidative polymerization of catechol. Such a laccase-mediator system allowed the formation ...

journal_title：Applied microbiology and biotechnology

authors： Kim S,Silva C,Evtuguin DV,Gamelas JA,Cavaco-Paulo A

更新日期：2011-02-01 00:00:00

abstract：:The genetic diversity and expression of amoA of autotrophic ammonia oxidizers in wastewater treatment processes were investigated by RT-PCR and denaturing gradient gel electrophoresis (DGGE) in order to identify active components of ammonia-oxidizer populations in a such processes. Ammonia oxidizers, evidenced by the ...

journal_title：Applied microbiology and biotechnology

authors： Ebie Y,Noda N,Miura H,Matsumura M,Tsuneda S,Hirata A,Inamori Y

更新日期：2004-06-01 00:00:00

abstract：:Cell-free preparations of Tetrahymena thermophila catalyze the direct desaturation of cholesterol to delta7-dehydrocholesterol (provitamin D3). The activity was isolated in the microsomal fraction from Tetrahymena homogenates. Delta7-desaturase activity was stimulated fivefold by the addition of 6 mM ATP. Other cofact...

journal_title：Applied microbiology and biotechnology

authors： Valcarce G,Florin-Christensen J,Nudel C

更新日期：2000-05-01 00:00:00

abstract：:Suspension cultures of the endemic South-African plant Cyclopia subternata were established for the first time and evaluated for the presence of isoflavones. The influence of light, as well as medium supplementation strategies with phenylalanine, casein hydrolysate and coconut water on biomass growth and isoflavone pr...

journal_title：Applied microbiology and biotechnology

authors： Kokotkiewicz A,Luczkiewicz M,Kowalski W,Badura A,Piekus N,Bucinski A

更新日期：2013-10-01 00:00:00