Isolation of a delta7-cholesterol desaturase from Tetrahymena thermophila.

Abstract:

:Cell-free preparations of Tetrahymena thermophila catalyze the direct desaturation of cholesterol to delta7-dehydrocholesterol (provitamin D3). The activity was isolated in the microsomal fraction from Tetrahymena homogenates. Delta7-desaturase activity was stimulated fivefold by the addition of 6 mM ATP. Other cofactors assayed, including NAD, NADP, NADH or NADPH, had no significant effect. The activity was found in microsomes prepared from stationary-phase cultures of the ciliate, grown either with or without added cholesterol, thus indicating that it is constitutively expressed in T. thermophila cells.

authors

Valcarce G,Florin-Christensen J,Nudel C

doi

10.1007/s002530051661

subject

Has Abstract

pub_date

2000-05-01 00:00:00

pages

591-5

issue

5

eissn

0175-7598

issn

1432-0614

journal_volume

53

pub_type

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