Phenotypic evaluation of natural and industrial Saccharomyces yeasts for different traits desirable in industrial bioethanol production.

abstract：:Fungi can grow in extreme habitats, such as natural stone and mineral building materials, sometimes causing deterioration. Efflorescence-concentrated salt deposits-results from water movement through building material; it can damage masonry materials and other bricks. Fungal isolate KUR1, capable of growth on, and dis...

journal_title：Applied microbiology and biotechnology

authors： Masaphy S,Lavi I,Sultz S,Zabari L

更新日期：2014-06-01 00:00:00

abstract：:In the course of more than 60-year history, penicillin G acylase (PGA) gained a unique position among enzymes used by pharmaceutical industry for production of β-lactam antibiotics. Kinetically controlled enzymatic syntheses of cephalosporins of novel generations in which PGA catalyzes coupling of activated acyl donor...

journal_title：Applied microbiology and biotechnology

authors： Marešová H,Plačková M,Grulich M,Kyslík P

更新日期：2014-04-01 00:00:00

abstract：:In this study, we investigated the possibility of using a modified hydantoinase process for the production of optically pure β-amino acids. Two aryl-substituted dihydropyrimidines D,L-6-phenyl-5,6-dihydrouracil (PheDU) and para-chloro-D,L-6-phenyl-5,6-dihydrouracil (pClPheDU) were synthesized. Hydrolysis of these nove...

journal_title：Applied microbiology and biotechnology

authors： Engel U,Syldatk C,Rudat J

更新日期：2012-06-01 00:00:00

abstract：:Real-time polymerase chain reaction (RT-PCR) was used to quantify seven species of lactic acid bacteria (LAB) in alfalfa silage prepared in the presence or absence of four commercial inoculants and in uninoculated corn stover harvested and stored under a variety of field conditions. Species-specific PCR primers were d...

journal_title：Applied microbiology and biotechnology

authors： Stevenson DM,Muck RE,Shinners KJ,Weimer PJ

更新日期：2006-07-01 00:00:00

abstract：:Cultivation of Aspergillus terreus ATCC 20542 in a stirred tank bioreactor was performed to induce the biosynthesis of secondary metabolites and provide the bioprocess-related insights into the metabolic capabilities of the investigated strain. The activation of biosynthetic routes was attempted by the diversification...

journal_title：Applied microbiology and biotechnology

authors： Boruta T,Bizukojc M

更新日期：2016-04-01 00:00:00

abstract：:Oleaginous yeasts (18 strains) were grown in ethanol media under various cultivation conditions (33 biomass samples). It was found that lipid and lipid-free fractions of dry biomass have elemental composition and biomass reductivity very close to values which can be considered as biological constants. The energy conte...

journal_title：Applied microbiology and biotechnology

authors： Minkevich IG,Dedyukhina EG,Chistyakova TI

更新日期：2010-10-01 00:00:00

abstract：:Toluene dioxygenase (TDO) catalyzes asymmetric cis-dihydroxylation of aromatic compounds. To achieve high efficient biotransformation of benzene to benzene cis-diols, Pseudomonas putida KT2442, Pseudomonas stutzeri 1317, and Aeromonas hydrophila 4AK4 were used as hosts to express TDO gene tod. Plasmid pSPM01, a deriva...

journal_title：Applied microbiology and biotechnology

authors： Ouyang SP,Sun SY,Liu Q,Chen J,Chen GQ

更新日期：2007-02-01 00:00:00

abstract：:D-lactic acid is used as a monomer in the production of poly-D-lactic acid (PDLA), which is used to form heat-resistant stereocomplex poly-lactic acid. To produce cost-effective D-lactic acid by using all sugars derived from biomass efficiently, xylose-assimilating genes encoding xylose isomerase and xylulokinase were...

journal_title：Applied microbiology and biotechnology

authors： Zhang Y,Vadlani PV,Kumar A,Hardwidge PR,Govind R,Tanaka T,Kondo A

更新日期：2016-01-01 00:00:00

abstract：:Agar is a major cell wall carbohydrate of red macroalgae (Rhodophyta). Sugars derived from agar, such as agarooligosaccharides (AOSs), neoagarooligosaccharides (NAOSs), neoagarobiose (NAB), and 3,6-anhydro-L-galactose (L-AHG), possess various physiological activities. These agar-derived sugars can be produced by hydro...

journal_title：Applied microbiology and biotechnology

authors： Yun EJ,Yu S,Kim KH

更新日期：2017-07-01 00:00:00

abstract：:To improve the stability and catalytic efficiency of pyranose 2-oxidase (P2Ox) by molecular enzyme evolution, we cloned P2Ox cDNA by RACE-PCR from a cDNA library derived from the basidiomycete Peniophora gigantea. The P2Ox gene was expressed in Escherichia coli BL21(DE3), yielding an intracellular and enzymatically ac...

journal_title：Applied microbiology and biotechnology

authors： Bastian S,Rekowski MJ,Witte K,Heckmann-Pohl DM,Giffhorn F

更新日期：2005-06-01 00:00:00

abstract：:Microbial aggregates of an aerobic granular sludge can be used for the treatment of industrial or municipal wastewater, but their formation from a microbial activated sludge requires several weeks. Therefore, the aim of this research was the selection of microbial cultures to shorten the granule-forming period from se...

journal_title：Applied microbiology and biotechnology

authors： Ivanov V,Wang XH,Tay ST,Tay JH

更新日期：2006-04-01 00:00:00

abstract：:During the genomics era, the use of thermostable DNA polymerases increased greatly. Many were identified and described-mainly of the genera Thermus, Thermococcus and Pyrococcus. Each polymerase has different features, resulting from origin and genetic modification. However, the rational choice of the adequate polymera...

journal_title：Applied microbiology and biotechnology

authors： Terpe K

更新日期：2013-12-01 00:00:00

abstract：:The natural genetic code only allows for 20 standard amino acids in protein translation, but genetic code reprogramming enables the incorporation of non-standard amino acids (NSAAs). Proteins containing NSAAs provide enhanced or novel properties and open diverse applications. With increased attention to the recent adv...

journal_title：Applied microbiology and biotechnology

authors： Jin X,Park OJ,Hong SH

更新日期：2019-04-01 00:00:00

abstract：:The extremely thermophilic anaerobic archaeon strain, HJ21, was isolated from a deep-sea hydrothermal vent, could produce hyperthermophilic alpha-amylase, and later was identified as Thermococcus from morphological, biochemical, and physiological characteristics and the 16S ribosomal RNA gene sequence. The extracellul...

journal_title：Applied microbiology and biotechnology

authors： Wang S,Lu Z,Lu M,Qin S,Liu H,Deng X,Lin Q,Chen J

更新日期：2008-09-01 00:00:00

abstract：:High poly(3-hydroxybutyrate) (PHB) content and volumetric productivity were achieved by fed-batch culture of Halomonas boliviensis using a defined medium. Initial shake flask cultivations in a minimal medium revealed that the growth of H. boliviensis was supported only when the medium was supplemented with aspartic ac...

journal_title：Applied microbiology and biotechnology

authors： Quillaguamán J,Doan-Van T,Guzmán H,Guzmán D,Martín J,Everest A,Hatti-Kaul R

更新日期：2008-02-01 00:00:00

abstract：:A heat-stable sarcosine oxidase produced by Bacillus sp. BSD-8 (SOX) had been studied and its complete gene sequence, which contained 1,164 bp nucleotides and encoded a protein of 387 amino acids, was obtained by DNA Walking method. The sox gene was cloned and functionally overexpressed in E. coli and the recombinant ...

journal_title：Applied microbiology and biotechnology

authors： Guo K,Ma X,Sun G,Zhao Y,Li X,Zhao W,Kai L

更新日期：2006-12-01 00:00:00

abstract：:The alkali-soluble glucan of the yeast cell wall contains beta-(1,3)- and (1,6)-D-linkages and systemically enhances the immune system. To isolate Saccharomyces cerevisiae mutants producing glucan with a high degree of beta-(1,6)-D-glycosidic bonds, a wild-type strain was mutagenized with ultraviolet light. The mutant...

journal_title：Applied microbiology and biotechnology

authors： Ha CH,Lim KH,Kim YT,Lim ST,Kim CW,Chang HI

更新日期：2002-03-01 00:00:00

abstract：:Shewanella decolorationis S12, a representative dissimilatory azo-reducing bacterium of Shewanella genus, can grow by coupling the oxidation of hydrogen to the reduction of azo compounds as the sole electron acceptor, indicating that an uptake hydrogenase is an important component for electron transfer for azoreductio...

journal_title：Applied microbiology and biotechnology

authors： Hong YG,Guo J,Sun GP

更新日期：2008-09-01 00:00:00

abstract：:The dibenzothiophene (DBT) desulfurizing operon, dsz, was introduced into various benzothiophene (BT)-desulfurizing bacteria using a Rhodococcus-E. coli shuttle vector. Of the tested recombinant bacteria, only those from Rhodococcus sp. strain T09 grew with both DBT and BT as the sole sulfur source. These recombinant ...

journal_title：Applied microbiology and biotechnology

authors： Matsui T,Hirasawa K,Konishi J,Tanaka Y,Maruhashi K,Kurane R

更新日期：2001-07-01 00:00:00

abstract：:We used biodegradable plastics as fermentation substrates for the filamentous fungus Aspergillus oryzae. This fungus could grow under culture conditions that contained emulsified poly-(butylene succinate) (PBS) and emulsified poly-(butylene succinate-co-adipate) (PBSA) as the sole carbon source, and could digest PBS a...

journal_title：Applied microbiology and biotechnology

authors： Maeda H,Yamagata Y,Abe K,Hasegawa F,Machida M,Ishioka R,Gomi K,Nakajima T

更新日期：2005-06-01 00:00:00

abstract：:His-Asn-His (HNH) proteins are a very common family of small nucleic acid-binding proteins that are generally associated with endonuclease activity and are found in all kingdoms of life. Although HNH endonucleases from mesophiles have been widely investigated, the biochemical functions of HNH endonucleases from thermo...

journal_title：Applied microbiology and biotechnology

authors： Zhang L,Huang Y,Xu D,Yang L,Qian K,Chang G,Gong Y,Zhou X,Ma K

更新日期：2016-09-01 00:00:00

abstract：:The behaviour of dry baker's yeast ( Saccharomyces cerevisiae type II, Sigma) used as biocatalyst without preliminary growth for the synthesis of 2-heptanol from 2-heptanone in a biphasic system is presented. Cells undergo intracellular trehalose consumption with a stoichiometric ethanol production during the first 15...

journal_title：Applied microbiology and biotechnology

authors： Cappaert L,Larroche C

更新日期：2004-06-01 00:00:00

abstract：:Peroxidases (PRX, EC 1.11.1.7) are widely distributed across microorganisms, plants, and animals; and, in plants, they have been implicated in a variety of secondary metabolic reactions. In particular, horseradish (Armoracia rusticana) root represents the main source of commercial PRX production. The prxC1a gene, whic...

journal_title：Applied microbiology and biotechnology

authors： Matsui T,Nakayama H,Yoshida K,Shinmyo A

更新日期：2003-10-01 00:00:00

abstract：:4AaCter is the polypeptide from the C-terminal extension of mosquitocidal Cry4Aa toxin, and facilitates formation of protein inclusion in Escherichia coli. It has been demonstrated that the use of 4AaCter as a peptide tag results in the efficient production of heterologous protein in E. coli. It has also been demonstr...

journal_title：Applied microbiology and biotechnology

authors： Hayakawa T,Shimizu Y,Ishida T,Sakai H

更新日期：2011-06-01 00:00:00

abstract：:The six- and seven-subunit Na+/H+ antiporters (Mrp) are widely distributed in bacteria. They are reported to be integral for pH homeostasis in alkaliphilic bacteria when adapting to high pH environments. In this study, operons encoding for the six-subunit Na+/H+ antiporters were found in the genomes of all studied Die...

journal_title：Applied microbiology and biotechnology

authors： Fang H,Qin XY,Zhang KD,Nie Y,Wu XL

更新日期：2018-04-01 00:00:00

abstract：:We in this study describe a new method for genomic studies of individual uncultured prokaryotic organisms, which was used for the isolation and partial genome sequencing of a soil archaeon. The diversity of Archaea in a soil sample was mapped by generating a clone library using group-specific primers in combination wi...

journal_title：Applied microbiology and biotechnology

authors： Kvist T,Ahring BK,Lasken RS,Westermann P

更新日期：2007-03-01 00:00:00

abstract：:Physiological heterogeneity constitutes a critical parameter in biotechnological systems since both metabolite yield and productivity are often hampered by the presence of undesired physiological cell subpopulations. In the present study, the physiological status and functionality of Pseudomonas taetrolens cells were ...

journal_title：Applied microbiology and biotechnology

authors： Alonso S,Rendueles M,Díaz M

更新日期：2012-12-01 00:00:00

abstract：:Epilactose (4-O-β-D-galactopyranosyl-D-mannose), an epimer of lactose, is a rare disaccharide existing extremely small quantities in heat-treated milk, in which epilactose is produced by non-enzymatic catalysis from lactose. This disaccharide is a kind of non-digestible carbohydrate, has a good prebiotic effect, and p...

journal_title：Applied microbiology and biotechnology

authors： Mu W,Li Q,Fan C,Zhou C,Jiang B

更新日期：2013-03-01 00:00:00

abstract：:Using molecular techniques and sequencing, we studied the intraspecific diversity of Oenococcus oeni, a lactic acid bacterium involved in red winemaking. A relationship between the phenotypic and genotypic characterization of 16 O. oeni strains isolated from wine with different levels of enological potential was shown...

journal_title：Applied microbiology and biotechnology

authors： Delaherche A,Bon E,Dupé A,Lucas M,Arveiler B,De Daruvar A,Lonvaud-Funel A

更新日期：2006-11-01 00:00:00

abstract：:Carvone, the principal component of spearmint oil, induces biodegradation of polychlorinated biphenyls (PCB) by Arthrobacter sp. strain B1B. This study investigated the effectiveness of the repeated application of carvone-induced bacteria for bioremediation of Aroclor-1242-contaminated soil. Control treatments compare...

journal_title：Applied microbiology and biotechnology

authors： Gilbert ES,Crowley DE

更新日期：1998-10-01 00:00:00