当前位置： SCI文献检索 > APPLIED MICROBIOLOGY AND BIOTECHNOLOGY期刊下所有文献 > Mutational analyses of Cry protein block7 polypeptides that facilitate the formation of protein inclusion in Escherichia coli.

Mutational analyses of Cry protein block7 polypeptides that facilitate the formation of protein inclusion in Escherichia coli.

Abstract：

:4AaCter is the polypeptide from the C-terminal extension of mosquitocidal Cry4Aa toxin, and facilitates formation of protein inclusion in Escherichia coli. It has been demonstrated that the use of 4AaCter as a peptide tag results in the efficient production of heterologous protein in E. coli. It has also been demonstrated that proteins are integrated, without losing their biological activities, into the protein inclusions. Although the mechanism to form protein inclusions in E. coli is unclear, highly conserved block7 sequence in 4AaCter is thought to be one of the functional factors. In this study, to analyze the ability of block7 to form protein inclusion, synthetic genes encoding the block7 polypeptide from selected 15 Cry proteins were constructed and expressed to produce glutathione S-transferase fusions in E. coli. Unexpectedly, only three of them (Cry5Ba, Cry32Aa, and Cry48Aa) formed protein inclusion as efficiently as that of Cry4Aa (>90% efficiency). The efficiencies in forming the protein inclusion were ranging from 39% to 66% for most of the tested block7s, and almost no protein inclusion was observed in Cry47Aa block7. This suggested that the ability of block7 to form the protein inclusion may vary with the type of Cry protein or the amino acid sequences. Mutational analyses revealed that substitution of the hydrophobic amino acids in block7 significantly affected the formation of protein inclusion, suggesting some important roles of these hydrophobic amino acid residues. Present results will contribute to develop a compact peptide tag based on block7 which forms the protein inclusion efficiently.

journal_name

Appl Microbiol Biotechnol

journal_title

Applied microbiology and biotechnology

authors

Hayakawa T,Shimizu Y,Ishida T,Sakai H

doi

10.1007/s00253-011-3216-4

subject

Has Abstract

pub_date

2011-06-01 00:00:00

pages

1943-51

issue

6

eissn

0175-7598

issn

1432-0614

journal_volume

90

pub_type

杂志文章

相关文献

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY文献大全
  • Streptomyces sp. JS520 produces exceptionally high quantities of undecylprodigiosin with antibacterial, antioxidative, and UV-protective properties.

    abstract：:A Gram-positive, red-pigment-producing bacterial strain, designated JS520 was isolated from the pristine sediment from the cave on mountain Miroc in Serbia. Strain was confirmed to belong to Streptomyces genus based on phenotypic and genetic analysis. Streptomyces sp. JS520 has the ability to produce exceptionally hig...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-012-4237-3

    authors： Stankovic N,Radulovic V,Petkovic M,Vuckovic I,Jadranin M,Vasiljevic B,Nikodinovic-Runic J

    更新日期：2012-12-01 00:00:00

  • The transcriptomic profile of Pseudozyma aphidis during production of mannosylerythritol lipids.

    abstract：:The basidiomycetous fungus Pseudozyma aphidis is able to convert vegetable oils to abundant amounts of the biosurfactant mannosylerythritol lipid (MEL) with a unique product pattern of MEL-A, MEL-B, MEL-C, and MEL-D. To investigate the metabolism of MEL production, we analyzed the transcriptome of P. aphidis DSM 70725...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-014-6359-2

    authors： Günther M,Grumaz C,Lorenz S,Stevens P,Lindemann E,Hirth T,Sohn K,Zibek S,Rupp S

    更新日期：2015-02-01 00:00:00

  • BioBrick™ compatible vector system for protein expression in Rhodobacter sphaeroides.

    abstract：:We report here the creation of a modular, plasmid-based protein expression system utilizing elements of the native Rhodobacter puf promoter in a BioBrick(TM)-based vector system with DsRed encoding a red fluorescent reporter protein. A suite of truncations of the puf promoter were made to assess the influence of diffe...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-014-5527-8

    authors： Tikh IB,Held M,Schmidt-Dannert C

    更新日期：2014-04-01 00:00:00

  • Metabolic engineering of Corynebacterium glutamicum for L-cysteine production.

    abstract：:L-cysteine, a valuable sulfur-containing amino acid, has been widely used in food, agriculture, and pharmaceutical industries. Due to the toxicity and complex regulation of L-cysteine, no efficient cell factory has yet been achieved for L-cysteine industrial production. In this study, the food-grade microorganism Cory...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-018-9547-7

    authors： Wei L,Wang H,Xu N,Zhou W,Ju J,Liu J,Ma Y

    更新日期：2019-02-01 00:00:00

  • Novel vitamin B12-producing Enterococcus spp. and preliminary in vitro evaluation of probiotic potentials.

    abstract：:Vitamin B12 is an essential nutrient required for crucial metabolic processes in humans. Vitamin B12-producing lactic acid bacteria (LAB) have been attracting increased attentions currently because of the generally recognized as safe (GRAS) status. Most of recent studies focused on Lactobacillus, and little is known a...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-017-8373-7

    authors： Li P,Gu Q,Wang Y,Yu Y,Yang L,Chen JV

    更新日期：2017-08-01 00:00:00

  • Increased intracellular calcium level and impaired nutrient absorption are important pathogenicity traits in the chicken intestinal epithelium during Campylobacter jejuni colonization.

    abstract：:Although a high number of chickens carry Campylobacter jejuni, the mechanistic action of colonization in the intestine is still poorly understood. The current study was therefore designed to investigate the effects of C. jejuni on glucose uptake, amino acids availability in digesta, and intracellular calcium [Ca(2+)]i...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-015-6543-z

    authors： Awad WA,Smorodchenko A,Hess C,Aschenbach JR,Molnár A,Dublecz K,Khayal B,Pohl EE,Hess M

    更新日期：2015-08-01 00:00:00

  • C16-Fengycin A affect the growth of Candida albicans by destroying its cell wall and accumulating reactive oxygen species.

    abstract：:Candida albicans is the most common clinical pathogenic fungus, which is highly susceptible to immunodeficiency. Development of novel antifungal agents has become a growing trend in the treatment of Candida infections. C16-Fengycin A, a lipopeptide isolated from Bacillus amyloliquefaciens fmb60 showed significant fung...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-019-10117-5

    authors： Liu Y,Lu J,Sun J,Zhu X,Zhou L,Lu Z,Lu Y

    更新日期：2019-11-01 00:00:00

  • Impact of protein uptake and degradation on recombinant protein secretion in yeast.

    abstract：:Protein titers, a key bioprocessing metric, depend both on the synthesis of protein and the degradation of protein. Secreted recombinant protein production in Saccharomyces cerevisiae is an attractive platform as minimal media can be used for cultivation, thus reducing fermentation costs and simplifying downstream pur...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-014-5783-7

    authors： Tyo KE,Liu Z,Magnusson Y,Petranovic D,Nielsen J

    更新日期：2014-08-01 00:00:00

  • Isolation of a bacterial consortium able to degrade the fungicide thiabendazole: the key role of a Sphingomonas phylotype.

    abstract：:Thiabendazole (TBZ) is a fungicide used in fruit-packaging plants. Its application leads to the production of wastewaters requiring detoxification. In the absence of efficient treatment methods, biological depuration of these effluents could be a viable alternative. However, nothing is known regarding the microbial de...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-017-8128-5

    authors： Perruchon C,Chatzinotas A,Omirou M,Vasileiadis S,Menkissoglou-Spiroudi U,Karpouzas DG

    更新日期：2017-05-01 00:00:00

  • Aroma compounds production by solid state fermentation, importance of in situ gas-phase recovery systems.

    abstract：:Flavour and fragrance compounds are extremely important for food, feed, cosmetic and pharmaceutical industries. In the last decades, due to the consumer's increased trend towards natural products, a great interest in natural aroma compounds has arisen to the detriment of chemically synthesised ones. Recently, solid st...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章,评审

    doi：10.1007/s00253-018-9157-4

    authors： Try S,Voilley A,Chunhieng T,De-Coninck J,Waché Y

    更新日期：2018-09-01 00:00:00

  • Applicability of pectate-entrapped Lactobacillus casei cells for L(+) lactic acid production from whey.

    abstract：:Lactic acid is a versatile organic acid, which finds major application in the food, pharmaceuticals, and chemical industries. Microbial fermentation has the advantage that by choosing a strain of lactic acid bacteria producing only one of the isomers, an optically pure product can be obtained. The production of L: (+)...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-006-0633-x

    authors： Panesar PS,Kennedy JF,Knill CJ,Kosseva MR

    更新日期：2007-02-01 00:00:00

  • Engineering Escherichia coli for efficient cellobiose utilization.

    abstract：:Escherichia coli normally cannot utilize the β-glucoside sugar cellobiose as a carbon and energy source unless a stringent selection pressure for survival is present. The cellobiose-utilization phenotype can be conferred by mutations in the two cryptic operons, chb and asc. In this study, the cellobiose-utilization ph...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-011-3434-9

    authors： Vinuselvi P,Lee SK

    更新日期：2011-10-01 00:00:00

  • Characterization of extracellular DNA production and flocculation of the marine photosynthetic bacterium Rhodovulum sulfidophilum.

    abstract：:The marine photosynthetic bacterium Rhodovulum sulfidophilum produces extracellular nucleic acids involved in its flocculation. Previously, we showed that the RNA fraction of these extracellular nucleic acids released into the culture medium contains mainly non-aminoacylated fully mature-sized tRNAs and fragments of 1...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-009-2031-7

    authors： Suzuki H,Daimon M,Awano T,Umekage S,Tanaka T,Kikuchi Y

    更新日期：2009-08-01 00:00:00

  • Amino acid substitutions in naphthalene dioxygenase from Pseudomonas sp. strain NCIB 9816-4 result in regio- and stereo-specific hydroxylation of flavanone and isoflavanone.

    abstract：:Wild-type naphthalene dioxygenase (NDO) from Pseudomonas sp. strain NCIB 9816-4 transforms relatively planar flavone and isoflavone to cis-dihydrodiols. However, this enzyme cannot catalyze the transformation of flavanone and isoflavanone in which a phenyl group bonds to the stereogenic C2 or C3 of the C-ring. Protein...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-012-3962-y

    authors： Seo J,Ryu JY,Han J,Ahn JH,Sadowsky MJ,Hur HG,Chong Y

    更新日期：2013-01-01 00:00:00

  • Recent developments in the taxonomic affiliation and phylogenetic positioning of fungi: impact in applied microbiology and environmental biotechnology.

    abstract：:The goal of modern taxonomy is to understand the relationships of living organisms in terms of evolutionary descent. Thereby, the relationships between living organisms are understood in terms of nested clades--every time a speciation event takes place, two new clades are produced. Life comprises three domains of livi...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章,评审

    doi：10.1007/s00253-011-3143-4

    authors： Voigt K,Kirk PM

    更新日期：2011-04-01 00:00:00

  • Application of 16S rRNA metagenomics to analyze bacterial communities at a respiratory care centre in Taiwan.

    abstract：:In this study, we applied a 16S ribosomal RNA (rRNA) metagenomics approach to survey inanimate hospital environments (IHEs) in a respiratory care center (RCC). A total of 16 samples, including 9 from medical devices and 7 from workstations, were analyzed. Besides, clinical isolates were retrospectively analyzed during...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-014-6176-7

    authors： Tang CY,Yiu SM,Kuo HY,Tan TS,Liao KH,Liu CC,Hon WK,Liou ML

    更新日期：2015-03-01 00:00:00

  • The occurrence of killer, sensitive, and neutral yeasts in Brazilian Riesling Italico grape must and the effect of neutral strains on killing behaviour.

    abstract：:The occurrence of killer toxins amongst yeast in Brazilian Riesling Italico grape must was investigated by using the sensitive strain EMBRAPA-26B as a reference strain at 18 degrees C and 28 degrees C. From a total of 85 previously isolated yeasts, 21 strains showed ability to kill the sensitive strain on unbuffered g...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s002530050791

    authors： da Silva GA

    更新日期：1996-09-01 00:00:00

  • Functional analysis of putative transporters involved in oligotrophic growth of Rhodococcus erythropolis N9T-4.

    abstract：:Rhodococcus erythropolis N9T-4, which is an extremely oligotrophic bacterium, can survive in a completely inorganic medium with no additional carbon source. This bacterium utilizes atmospheric CO2, but does not require any additional energy source such as light and hydrogen gas, required by autotrophic microorganisms....

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-019-09714-1

    authors： Matsuoka T,Yoshida N

    更新日期：2019-05-01 00:00:00

  • Microbial oxidation of ebastine.

    abstract：:The microbial oxidation of ebastine to carebastine was investigated. Among the 15 micro-organisms examined, only the Cunninghamella strains showed the desired biotransformation. Cunninghamella blakesleeana oxidised the substrate within 7 days, via the intermediates alcohol and aldehyde, mainly to carebastine, the corr...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/BF00178610

    authors： Schwartz H,Liebig-Weber A,Hochstätter H,Böttcher H

    更新日期：1996-02-01 00:00:00

  • Biodegradation of textile azo dye by Shewanella decolorationis S12 under microaerophilic conditions.

    abstract：:The complete biodegradation of azo dye, Fast Acid Red GR, was observed under microaerophilic conditions by Shewanella decolorationis S12. Although the highest decolorizing rate was measured under anaerobic condition and the highest biomass was obtained under aerobic condition, a further biodegradation of decolorizing ...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-007-1032-7

    authors： Xu M,Guo J,Sun G

    更新日期：2007-09-01 00:00:00

  • Construction of new Pichia pastoris X-33 strains for production of lycopene and β-carotene.

    abstract：:In this study, we used the non-carotenogenic yeast Pichia pastoris X33 as a receptor for β-carotene-encoding genes, in order to obtain new recombinant strains capable of producing different carotenoidic compounds. We designed and constructed two plasmids, pGAPZA-EBI* and pGAPZA-EBI*L*, containing the genes encoding ly...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-011-3764-7

    authors： Araya-Garay JM,Feijoo-Siota L,Rosa-dos-Santos F,Veiga-Crespo P,Villa TG

    更新日期：2012-03-01 00:00:00

  • Biosynthesis of bioactive O-methylated flavonoids in Escherichia coli.

    abstract：:Two bioactive O-methylflavonoids, sakuranetin (7-O-methylnaringenin) and ponciretin (7-O-methylnaringenin), were synthesized in Escherichia coli. Sakuranetin inhibits germination of Magnaporthe grisea, and ponciretin is a potential inhibitor of Helicobacter pylori. To achieve this, we reconstructed the naringenin bios...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-013-5020-9

    authors： Kim MJ,Kim BG,Ahn JH

    更新日期：2013-08-01 00:00:00

  • High-level expression of a truncated 1,3-1,4-beta-D-glucanase from Fibrobacter succinogenes in Pichia pastoris by optimization of codons and fermentation.

    abstract：:1,3-1,4-beta-D-glucanase is an important endoglycosidase in the brewing and animal feed industries. To achieve high-level expression of recombinant glucanase in Pichia pastoris, we designed sequences encoding the alpha-factor signal peptide from Saccharomyces cerevisiae and the truncated 1,3-1,4-beta-D-glucanase from ...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-007-1290-4

    authors： Huang H,Yang P,Luo H,Tang H,Shao N,Yuan T,Wang Y,Bai Y,Yao B

    更新日期：2008-02-01 00:00:00

  • Establishment of a hyper-protein production system in submerged Aspergillus oryzae culture under tyrosinase-encoding gene (melO) promoter control.

    abstract：:UV-mediated mutagenesis generated a high glucoamylase-producing mutant of Aspergillus oryzae exhibiting strong melanization in solid-state culture. Expression of the glucoamylase-encoding gene (glaB), which is specifically expressed in solid-state culture, and the tyrosinase-encoding gene (melO), was analyzed using an...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s002530100771

    authors： Ishida H,Matsumura K,Hata Y,Kawato A,Suginami K,Abe Y,Imayasu S,Ichishima E

    更新日期：2001-10-01 00:00:00

  • Environmental evaluation of coexistence of denitrifying anaerobic methane-oxidizing archaea and bacteria in a paddy field.

    abstract：:The nitrate-dependent denitrifying anaerobic methane oxidation (DAMO) process, which is metabolized together by anaerobic methanotrophic archaea and NC10 phylum bacteria, is expected to be important for the global carbon and nitrogen cycles. However, there are little studies about the existence of this process and the...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-015-6986-2

    authors： Ding J,Fu L,Ding ZW,Lu YZ,Cheng SH,Zeng RJ

    更新日期：2016-01-01 00:00:00

  • Functions and impact of tal-like genes in animals with regard to applied aspects.

    abstract：:A large number of DNAs in eukaryote genomes can code for atypical transcripts, and their functions are controversial. It has been reported that the transcripts contain many small open reading frames (sORFs), which were originally considered as non-translatable RNAs. However, increasing evidence has suggested that some...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章,评审

    doi：10.1007/s00253-018-9159-2

    authors： Zhu M,Hu X,Cao G,Xue R,Gong C

    更新日期：2018-08-01 00:00:00

  • New available SCAR markers: potentially useful in distinguishing a commercial strain of the superior type from other strains of Lentinula edodes in China.

    abstract：:At present, more than 100 strains of Lentinula edodes are cultivated on a commercial scale in China. A simple, reliable, and effective method to distinguish some commercial strains of the superior type from other commercial strains is very important for the Lentinula industry. In this study, 23 commercial strains of L...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-008-1671-3

    authors： Li HB,Wu XQ,Peng HZ,Fu LZ,Wei HL,Wu QQ,Jin QY,Li N

    更新日期：2008-11-01 00:00:00

  • Comparative analyses of Podospora anserina secretomes reveal a large array of lignocellulose-active enzymes.

    abstract：:The genome of the coprophilous fungus Podospora anserina harbors a large and highly diverse set of putative lignocellulose-acting enzymes. In this study, we investigated the enzymatic diversity of a broad range of P. anserina secretomes induced by various carbon sources (dextrin, glucose, xylose, arabinose, lactose, c...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-014-5698-3

    authors： Poidevin L,Berrin JG,Bennati-Granier C,Levasseur A,Herpoël-Gimbert I,Chevret D,Coutinho PM,Henrissat B,Heiss-Blanquet S,Record E

    更新日期：2014-09-01 00:00:00

  • Methods for visualising active microbial benzene degraders in in situ microcosms.

    abstract：:Natural attenuation maybe a cost-efficient option for bioremediation of contaminated sites but requires knowledge about the activity of degrading microbes under in situ conditions. In order to link microbial activity to the spatial distribution of contaminant degraders, we combined the recently improved in situ microc...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-014-6037-4

    authors： Schurig C,Mueller CW,Höschen C,Prager A,Kothe E,Beck H,Miltner A,Kästner M

    更新日期：2015-01-01 00:00:00

  • Production of recombinant mink growth hormone in E. coli.

    abstract：:Escherichia coli cells expressing mink (Mustela vison) growth hormone were grown in a batch fermentation process. The expression level was estimated to be 27% of the total cellular protein after 3 h of induction with 1 mM isopropyl beta-D-thiogalactoside (IPTG). If the expression of mink growth hormone (mGH) was induc...

    journal_title：Applied microbiology and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s00253-006-0673-2

    authors： Sereikaite J,Statkute A,Morkunas M,Radzevicius K,Borromeo V,Secchi C,Bumelis VA

    更新日期：2007-02-01 00:00:00