Incorporation of non-standard amino acids into proteins: challenges, recent achievements, and emerging applications.

abstract：:The Gram-positive bacterium, Bacillus subtilis and related species are widely used industrially as hosts for producing enzymes. These species possess a high potential to produce secreted proteins into the culture medium. Nevertheless, the secretion of heterologous proteins by these species is frequently inefficient. I...

journal_title：Applied microbiology and biotechnology

authors： Kakeshita H,Kageyama Y,Ara K,Ozaki K,Nakamura K

更新日期：2011-03-01 00:00:00

abstract：:Mitochondrial dysfunction in Saccharomyces cerevisiae was selected as a marker of ion penetration following carbon ion beam (CIB) irradiation. Respiration-deficient mutants were screened. Following confirmation of negligible spontaneous mutation, eight genetically stable S. cerevisiae respiration-deficient mutant stra...

journal_title：Applied microbiology and biotechnology

authors： Guo X,Zhang M,Gao Y,Cao G,Yang Y,Lu D,Li W

更新日期：2019-02-01 00:00:00

abstract：:A Saccharomyces-cerevisiae-based simultaneous saccharification and fermentation (SSF) of lignocellulosic biomass is limited to an operating temperature of about 37 degrees C, and even a small increase in temperature can have a deleterious effect. This points to a need for a more thermotolerant yeast. To this end, S. c...

journal_title：Applied microbiology and biotechnology

authors： Kadam KL,Schmidt SL

更新日期：1997-12-01 00:00:00

abstract：:D-Amino acids exist widely in microbes, plants, animals, and food and can be applied in pharmaceutical, food, and cosmetics. Because of their widespread applications in industry, D-amino acids have recently received more and more attention. Enzymes including D-hydantoinase, N-acyl-D-amino acid amidohydrolase, D-amino ...

journal_title：Applied microbiology and biotechnology

authors： Gao X,Ma Q,Zhu H

更新日期：2015-04-01 00:00:00

abstract：:Increasing evidence suggests that maintenance of homeostasis between gut microbiota and host plays an important role in human health. Many diseases, such as those affecting the liver, have been linked to imbalances in gut microbial communities. However, it is not clear whether an imbalance in gut microbiota promotes t...

journal_title：Applied microbiology and biotechnology

authors： Hu X,Wang T,Liang S,Li W,Wu X,Jin F

更新日期：2015-11-01 00:00:00

abstract：:Acrylamide is an important bulk chemical used for producing polyacrylamide, which is widely applied in diverse fields, such as enhanced oil recovery and water treatment. Acrylamide production with a superior biocatalyst, free-resting Rhodococcus cells containing nitrile hydratase (NHase), has been proven to be simple ...

journal_title：Applied microbiology and biotechnology

authors： Jiao S,Li F,Yu H,Shen Z

更新日期：2020-02-01 00:00:00

abstract：:Lipoxygenase (LOX; EC 1.13.11.12) is an enzyme which is widely used in food industry to improve aroma and rheological or baking properties of foods. A series of studies have proven that the flexible regions negatively relates to the thermal stability of enzymes. In this study, two highly flexible regions, residues(20-...

journal_title：Applied microbiology and biotechnology

authors： Lu X,Liu S,Feng Y,Rao S,Zhou X,Wang M,Du G,Chen J

更新日期：2014-02-01 00:00:00

abstract：:The gene from Xanthomonas campestris pv. phaseoli that is involved in the C5 pathway of delta-amino-levulinic acid (ALA) of Escherichia coli. Subcloning of deletion fragments from the initial 2.5-kilobase (kb) chromosomal fragment allowed the isolation of a 1.6-kb fragment that could complement the hemM mutation. Nucl...

journal_title：Applied microbiology and biotechnology

authors： Asahara N,Murakami K,Korbrisate S,Hashimoto Y,Murooka Y

更新日期：1994-02-01 00:00:00

abstract：:β-Glucosidases designated MoCel3A and MoCel3B were successfully overexpressed in Magnaporthe oryzae. MoCel3A and MoCel3B showed optimal activity at 50 °C and pH 5.0-5.5. MoCel3A exhibited higher activity on higher degree of polymerization (DP) oligosaccharides and on β-1,3-linked oligosaccharides than on β-1,4-linked ...

journal_title：Applied microbiology and biotechnology

authors： Takahashi M,Konishi T,Takeda T

更新日期：2011-08-01 00:00:00

abstract：:As the initial step in a project to provide a more cost-effective source of the phytase enzyme, this paper reports on the use of a polyclonal antibody raised to phytase purified from an isolate of Aspergillus niger (A. ficuum) to screen an A. niger lambda gt11 expression library and the use of amino acid sequencing to...

journal_title：Applied microbiology and biotechnology

authors： Mullaney EJ,Gibson DM,Ullah AH

更新日期：1991-08-01 00:00:00

abstract：:In order for microalgae-based processes to reach commercial production for biofuels and high-value products such as omega-3 fatty acids, it is necessary that economic feasibility be demonstrated at the industrial scale. Therefore, process optimization is critical to ensure that the maximum yield can be achieved from t...

journal_title：Applied microbiology and biotechnology

authors： Lowrey J,Armenta RE,Brooks MS

更新日期：2016-02-01 00:00:00

abstract：:Taxol is an anticancer identified in both endophytic fungus and its host plant. Plant Taxol is a diterpenoid with geranylgeranyl diphosphate (GGPP) mediates the biosynthesis of its terpenoid moiety. Previous report has suggested that fungal Taxol may require terpenoid pathway for its biosynthesis. Here in this study, ...

journal_title：Applied microbiology and biotechnology

authors： Soliman SSM,Mosa KA,El-Keblawy AA,Husseiny MI

更新日期：2017-10-01 00:00:00

abstract：:Extracellular polymeric substances (EPS) play an important role in the formation and activity of biofilms in wastewater treatment (WWT). The EPS of the denitrifying biomarker Comamonas denitrificans strain 110, produced in different culture media and growth modes, were characterized. The EPS mainly contained protein (...

journal_title：Applied microbiology and biotechnology

authors： Andersson S,Dalhammar G,Land CJ,Kuttuva Rajarao G

更新日期：2009-03-01 00:00:00

abstract：:An enzyme preparation from the thermophilic fungus Humicola insolens, Ultraflo L, was able to solubilise more than half of the biomass of brewer's grain and wheat bran, two agro-industrial co-products. While almost all of the ferulic acid was released in the free form, the majority of diferulates were released still a...

journal_title：Applied microbiology and biotechnology

authors： Faulds CB,Mandalari G,LoCurto R,Bisignano G,Waldron KW

更新日期：2004-06-01 00:00:00

abstract：:The membraneless bioelectrochemical reactor (Ml-BER) is useful for dark hydrogen fermentation. The effect of the electrochemical reaction on microorganisms in the Ml-BER was investigated using glucose as the substrate and compared with organisms in a membraneless non-bioelectrochemical reactor (Ml-NBER) and bioelectro...

journal_title：Applied microbiology and biotechnology

authors： Sasaki K,Morita M,Sasaki D,Ohmura N,Igarashi Y

更新日期：2013-08-01 00:00:00

abstract：:Saccharomyces cerevisiae is the organism of choice for many food and beverage fermentations because it thrives in high-sugar and high-ethanol conditions. However, the conditions encountered in bioethanol fermentation pose specific challenges, including extremely high sugar and ethanol concentrations, high temperature,...

journal_title：Applied microbiology and biotechnology

authors： Mukherjee V,Steensels J,Lievens B,Van de Voorde I,Verplaetse A,Aerts G,Willems KA,Thevelein JM,Verstrepen KJ,Ruyters S

更新日期：2014-11-01 00:00:00

abstract：:Maltol derivatives are utilized in a variety of fields due to their metal-chelating abilities, and modification of the 2-methyl side chain is known to effectively expand their functional diversity. In the present study, microbial enzymes were screened for hydroxylating activity towards the 2-methyl group in a maltol d...

journal_title：Applied microbiology and biotechnology

authors： Kozono I,Mihara K,Minagawa K,Hibi M,Ogawa J

更新日期：2017-09-01 00:00:00

abstract：:In the Candida antarctica lipase B-catalyzed hydrolysis of (R,S)-azolides derived from (R,S)-N-protected proline in water-saturated methyl tert-butyl ether (MTBE), high enzyme activity with excellent enantioselectivity (V (S) V (R) ...

journal_title：Applied microbiology and biotechnology

authors： Wu CH,Pen CW,Wang PY,Tsai SW

更新日期：2013-02-01 00:00:00

abstract：:This study found that the ratio of Thiothrix eikelboomii to total bacterial concentrations (TH/TB) (%) was a better indicator of bulking incidents affecting effluent quality compared to absolute T. eikelboomii abundance alone. This was determined using a genus-specific Thiothrix quantitative PCR primer and probe set, ...

journal_title：Applied microbiology and biotechnology

authors： Asvapathanagul P,Olson BH,Gedalanga PB,Hashemi A,Huang Z,La J

更新日期：2015-05-01 00:00:00

abstract：:The production of the anticoagulant drug heparin from non-animal sources has a number of advantages over the current commercial production of heparin. These advantages include better source material availability, improved quality control, and reduced concerns about animal virus or prion impurities. A bioengineered hep...

journal_title：Applied microbiology and biotechnology

authors： Wang Z,Yang B,Zhang Z,Ly M,Takieddin M,Mousa S,Liu J,Dordick JS,Linhardt RJ

更新日期：2011-07-01 00:00:00

abstract：:A 6-kDa antifungal peptide with inhibitory activity on mycelial growth in Fusarium oxysporum, Mycosphaerella arachidicola, and Physalospora piricola was isolated from baby lima beans. The peptide suppressed growth in M. arachidicola with an IC(50) of 0.87 muM and inhibited activity of HIV-1 reverse transcriptase with ...

journal_title：Applied microbiology and biotechnology

authors： Wang HX,Ng TB

更新日期：2006-12-01 00:00:00

abstract：:Atrazine is transformed to N-isopropylammelide through hydroxyatrazine as an intermediate as indicated by high-performance liquid chromatography/mass spectroscopy in culture filtrates of Frankia alni ACN14a and Frankia sp. EuI1c. Both Frankia strains have the ability to degrade atrazine via dechlorination and dealkyla...

journal_title：Applied microbiology and biotechnology

authors： Rehan M,Kluge M,Fränzle S,Kellner H,Ullrich R,Hofrichter M

更新日期：2014-07-01 00:00:00

abstract：:Enterokinase is one of the most frequently used enzymes for the removal of affinity tags from target recombinant proteins. In this study, several fermentation strategies were assayed for the production of human enterokinase in Pichia pastoris under constitutive GAP promoter. Two of them with controlled specific growth...

journal_title：Applied microbiology and biotechnology

authors： Melicherová K,Krahulec J,Šafránek M,Lišková V,Hopková D,Széliová D,Turňa J

更新日期：2017-03-01 00:00:00

abstract：:The use of probiotics to treat gastrointestinal diseases such as diarrhea especially in children is becoming increasingly popular. Besides, the use of nanomaterials in food products is increasing rapidly especially in candies and chocolates. How these nanomaterials influence probiotic bacteria and their activity remai...

journal_title：Applied microbiology and biotechnology

authors： Khan ST,Saleem S,Ahamed M,Ahmad J

更新日期：2019-08-01 00:00:00

abstract：:In this study, we investigated the possibility of using a modified hydantoinase process for the production of optically pure β-amino acids. Two aryl-substituted dihydropyrimidines D,L-6-phenyl-5,6-dihydrouracil (PheDU) and para-chloro-D,L-6-phenyl-5,6-dihydrouracil (pClPheDU) were synthesized. Hydrolysis of these nove...

journal_title：Applied microbiology and biotechnology

authors： Engel U,Syldatk C,Rudat J

更新日期：2012-06-01 00:00:00

abstract：:The tandem multimeric expression of various peptides has been explored by many researchers. However, expression levels have usually not been proportional to the degree of multimerization. To increase the expression level in Escherichia coli of tandem multimers of a cationic antimicrobial peptide, buforin II, fused to ...

journal_title：Applied microbiology and biotechnology

authors： Lee JH,Kim MS,Cho JH,Kim SC

更新日期：2002-05-01 00:00:00

abstract：:The human granulocyte macrophage colony-stimulating factor (GM-CSF) is a glycoprotein with important clinical applications for the treatment of neutropenia and aplastic anemia and reducing infections associated with bone marrow transplants. We evaluated the potential for using a potato virus X (PVX) viral vector syste...

journal_title：Applied microbiology and biotechnology

authors： Zhou F,Wang ML,Albert HH,Moore PH,Zhu YJ

更新日期：2006-10-01 00:00:00

abstract：:The medicinal mushroom Agaricus blazei produced high amounts of laccase (up to 5,000 units l(-1)) in a complex, agitated liquid medium based on tomato juice, while only traces of the enzyme (<100 units l(-1)) were detected in synthetic glucose-based medium. Purification of the enzyme required three chromatographic ste...

journal_title：Applied microbiology and biotechnology

authors： Ullrich R,Huong le M,Dung NL,Hofrichter M

更新日期：2005-05-01 00:00:00

abstract：:The utilization of monochlorobenzoate isomers (2-, 3- and 4-chlorobenzoate) by anaerobic microbial consortia in River Nile sediments was systematically evaluated under denitrifying, Fe-reducing, sulfidogenic and methanogenic conditions. Loss of all three chlorobenzoates was noted in denitrifying cultures; furthermore,...

journal_title：Applied microbiology and biotechnology

authors： Kazumi J,Häggblom MM,Young LY

更新日期：1995-10-01 00:00:00

abstract：:Candida albicans is the most common clinical pathogenic fungus, which is highly susceptible to immunodeficiency. Development of novel antifungal agents has become a growing trend in the treatment of Candida infections. C16-Fengycin A, a lipopeptide isolated from Bacillus amyloliquefaciens fmb60 showed significant fung...

journal_title：Applied microbiology and biotechnology

authors： Liu Y,Lu J,Sun J,Zhu X,Zhou L,Lu Z,Lu Y

更新日期：2019-11-01 00:00:00