A novel approach to microbial breeding--low-energy ion implantation.

abstract：:Engineered S. cerevisiae employing the xylose reductase pathway enables efficient xylose valorization to fuels and chemicals. However, toxicity of thermochemically pretreated biomass hydrolysate on S. cerevisiae is one of the key technical challenges to upgrade biomass-derived sugars including xylose and glucose into ...

journal_title：Applied microbiology and biotechnology

authors： Jayakody LN,Turner TL,Yun EJ,Kong II,Liu JJ,Jin YS

更新日期：2018-09-01 00:00:00

abstract：:The synthesis of rhamnosylated compounds has gained great importance since these compounds have potential therapeutic applications. The enzymatic approaches for glycosylation of bioactive molecules have been well developed; however, the enzymatic rhamnosylation has been largely hindered by lacking of the glycosyl dono...

journal_title：Applied microbiology and biotechnology

authors： Xu L,Liu X,Li Y,Yin Z,Jin L,Lu L,Qu J,Xiao M

更新日期：2019-10-01 00:00:00

abstract：:Cultivation processes involving filamentous fungi have been optimised for decades to obtain high product yields. Several bulk chemicals like citric acid and penicillin are produced this way. A simple adaptation of cultivation parameters for new production processes is not possible though. Models explaining the correla...

journal_title：Applied microbiology and biotechnology

authors： Grimm LH,Kelly S,Krull R,Hempel DC

更新日期：2005-12-01 00:00:00

abstract：:Using genetic hybridization analysis, pulsed-field gel electrophoresis of chromosomal DNA and PCR/RFLP analysis of the MET2 gene, we reidentified 11 Champagne yeast strains. Two of them, SCPP and SC4, were found to belong to Saccharomyces bayanus var. uvarum and the remaining strains to S. cerevisiae. Strain ...

journal_title：Applied microbiology and biotechnology

authors： Naumov GI,Naumova ES,Aigle M,Masneuf I,Belarbi A

更新日期：2001-01-01 00:00:00

abstract：:meta-Cleavage product (MCP) hydrolases can catalyze relatively low reactive carbon-carbon bond hydrolysis of products, which are derived from the meta-cleavage of catechols. The strict substrate selectivity of MCP hydrolases attracts an interest to understand the determinants of substrate specificity. Compared with co...

journal_title：Applied microbiology and biotechnology

authors： Zhou H,Qu Y,Shen E,Kong C,Zhang X,Ma Q,Zhou J

更新日期：2013-06-01 00:00:00

abstract：:Prenyltransferases of the dimethylallyltryptophan synthase (DMATS) superfamily are involved in the biosynthesis of secondary metabolites and show broad substrate specificity towards their aromatic substrates with a high regioselectivity for the prenylation reactions. Most members of this superfamily accepted as prenyl...

journal_title：Applied microbiology and biotechnology

authors： Pockrandt D,Sack C,Kosiol T,Li SM

更新日期：2014-06-01 00:00:00

abstract：:The suitability of culture supernatant from Streptomyces albus ATCC 3005 for use in the biobleaching of eucalyptus kraft pulp was investigated. S. albus was found to grow on a minimal salts medium containing oat spelts xylan and yeast extract as the main carbon and nitrogen sources, respectively. Maximal extracellular...

journal_title：Applied microbiology and biotechnology

authors： Antonopoulos VT,Hernandez M,Arias ME,Mavrakos E,Ball AS

更新日期：2001-10-01 00:00:00

abstract：:The formation and activity of an As(III)-oxidising biofilm in a bioreactor, using pozzolana as bacterial growth support, was studied for the purpose of optimising fixed-bed bioreactors for bioremediation. After 60 days of continuous functioning with an As(III)-contaminated effluent, the active biofilm was found to be ...

journal_title：Applied microbiology and biotechnology

authors： Michel C,Jean M,Coulon S,Dictor MC,Delorme F,Morin D,Garrido F

更新日期：2007-11-01 00:00:00

abstract：:After analysis of batch culture and identification of the ways for prolongation of citric acid active synthesis by yeast, repeat-batch (RB) cultivation was suggested. Yarrowia lipolytica strain RB cultivation was studied and optimal conditions for cultivation selected. It was shown that when applying RB cultivation, b...

journal_title：Applied microbiology and biotechnology

authors： Arzumanov TE,Shishkanova NV,Finogenova TV

更新日期：2000-05-01 00:00:00

abstract：:The saprotrophic fungus Penicillium griseofulvum was chosen as model organism to study responses to a mixture of hexachlorocyclohexane (HCH) isomers (α-HCH, β-HCH, γ-HCH, δ-HCH) and potentially toxic metals (vanadium, lead) in solid and liquid media. The P. griseofulvum FBL 500 strain was isolated from polluted soil c...

journal_title：Applied microbiology and biotechnology

authors： Ceci A,Pinzari F,Riccardi C,Maggi O,Pierro L,Petrangeli Papini M,Gadd GM,Persiani AM

更新日期：2018-01-01 00:00:00

abstract：:Four types of cell-surface-engineered yeast Saccharomyces cerevisiae displaying glucoamylase, namely, systems A, B, C, and D, were constructed to evaluate their performance in direct ethanol fermentation from raw corn starch. Systems A and B were glucoamylase-displaying nonflocculent yeast (YF237) types that secrete a...

journal_title：Applied microbiology and biotechnology

authors： Khaw TS,Katakura Y,Koh J,Kondo A,Ueda M,Shioya S

更新日期：2006-05-01 00:00:00

abstract：:MoHrip2, identified from Magnaporthe oryzae as an elicitor, can activate plant defense responses either in the form of recombinant protein in vitro or ectopic expressed protein in rice. However, its intrinsic function in the infective interaction of M. oryzae-rice is largely unknown. Here, we found that mohrip2 expres...

journal_title：Applied microbiology and biotechnology

authors： Nie H,Zhang L,Zhuang H,Yang X,Qiu D,Zeng H

更新日期：2019-08-01 00:00:00

abstract：:Biosurfactant rhamnolipids have been claimed to show biological activities of inhibiting the proliferation of cancer cells. In this study, the cytotoxicity of rhamnolipids was examined on four cancer cells (HepG2, Caco-2, Hela, MCF-7 cells) and two normal cells (HK-2 cell, primary hepatocyte). Interestingly, both canc...

journal_title：Applied microbiology and biotechnology

authors： Jiang L,Shen C,Long X,Zhang G,Meng Q

更新日期：2014-12-01 00:00:00

abstract：:In contrast to stringent (relA+) cells of Escherichia coli, relaxed (relA) cells excreted recombinant proteins (beta-lactamase, interferon alpha 1) into the culture medium during amino acid limitation. Comparative analyses of overall fatty acid composition in relA+ cells and relA cells were performed and revealed that...

journal_title：Applied microbiology and biotechnology

authors： Gitter B,Diefenbach R,Keweloh H,Riesenberg D

更新日期：1995-04-01 00:00:00

abstract：:Corynebacterium glutamicum ORF NCgl0328, designated noxA, encodes an NADH oxidase enzyme. The noxA gene, which was preferentially expressed in the log growth phase, was found to be under the control of the whcA, whcB, and whcE genes, which play regulatory roles in cells under oxidative stress. While noxA transcription...

journal_title：Applied microbiology and biotechnology

authors： Park JC,Kim Y,Lee HS

更新日期：2015-02-01 00:00:00

abstract：:Nitrite-dependent anaerobic methane oxidation (N-DAMO), which couples anaerobic methane oxidation and nitrite reduction, is a recently discovered bioprocess coupling microbial nitrogen and carbon cycles. The discovery of this microbial process challenges the traditional knowledge of global methane sinks and nitrogen l...

journal_title：Applied microbiology and biotechnology

authors： Wang J,Shen L,He Z,Hu J,Cai Z,Zheng P,Hu B

更新日期：2017-11-01 00:00:00

abstract：:Rhizopus oryzae is a filamentous fungus belonging to the Zygomycetes. It is among others known for its ability to produce the sustainable platform chemicals L: -(+)-lactic acid, fumaric acid, and ethanol. During glycolysis, all fermentable carbon sources are metabolized to pyruvate and subsequently distributed over th...

journal_title：Applied microbiology and biotechnology

authors： Meussen BJ,de Graaff LH,Sanders JP,Weusthuis RA

更新日期：2012-05-01 00:00:00

abstract：:Histone lysine acetylation orchestrates transcriptional activity essential for diverse cellular events across organisms, but it remains poorly understood how an acetylated lysine affects cellular functions in filamentous fungal pathogens. Here, we show the functions of a histone acetyltransferase that is phylogenetica...

journal_title：Applied microbiology and biotechnology

authors： Wang JJ,Cai Q,Qiu L,Ying SH,Feng MG

更新日期：2018-02-01 00:00:00

abstract：:Yeast are important production platforms for the generation of recombinant proteins. Nonetheless, their use has been restricted in the production of therapeutic proteins due to differences in their glycosylation profile with that of higher eukaryotes. The yeast strain Pichia pastoris is an industrially important organ...

journal_title：Applied microbiology and biotechnology

authors： Hopkins D,Gomathinayagam S,Lynaugh H,Stadheim TA,Hamilton SR

更新日期：2014-03-01 00:00:00

abstract：:Cyclodipeptide oxidases (CDOs) perform dehydrogenations on diketopiperazines and play an important role in the cyclodipeptide diversification. In this study, we investigated the two known CDOs AlbA/B and Ndas_1146/7 and one new member, CDO-Np. LC-MS monitoring of 32 cyclodipeptide biotransformations in E. coli reveale...

journal_title：Applied microbiology and biotechnology

authors： Mikulski L,Schäfer J,Brockmeyer K,Kraut R,Li SM

更新日期：2020-03-01 00:00:00

abstract：:Economical production of medium-chain length polyhydroxyalkanoates (mcl-PHA) is dependent on efficient cultivation processes. This work describes growth and mcl-PHA synthesis characteristics of Pseudomonas putida LS46 when grown on medium-chain length fatty acids (octanoic acid) and lower-cost long-chain fatty acids (...

journal_title：Applied microbiology and biotechnology

authors： Blunt W,Dartiailh C,Sparling R,Gapes D,Levin DB,Cicek N

更新日期：2018-08-01 00:00:00

abstract：:By using immobilized baker's yeast repeatedly in isooctane with occasional reactivation by cultivation, we succeeded in the preparation of an organic solvent-tolerant strain, named KK21, which could grow in the presence of isooctane. This is the first report on an organic solvent-tolerant strain from baker's yeast. St...

journal_title：Applied microbiology and biotechnology

authors： Kawamoto T,Kanda T,Tanaka A

更新日期：2001-05-01 00:00:00

abstract：:Application of soil amendments has been wildly used to increase soil pH and control bacterial wilt. However, little is known about causal shifts in the rhizosphere microbial community of crops, especially when the field naturally harbors the disease of bacterial wilt to tobacco for many years due to long-term continuo...

journal_title：Applied microbiology and biotechnology

authors： Shen G,Zhang S,Liu X,Jiang Q,Ding W

更新日期：2018-11-01 00:00:00

abstract：:Pseudomonas putida KT2440 grew on glucose at a specific rate of 0.48 h(-1) but accumulated almost no poly-3-hydroxyalkanoates (PHA). Subsequent nitrogen limitation on nonanoic acid resulted in the accumulation of only 27% medium-chain-length PHA (MCL-PHA). In contrast, exponential nonanoic acid-limited growth (mu = 0....

journal_title：Applied microbiology and biotechnology

authors： Sun Z,Ramsay JA,Guay M,Ramsay BA

更新日期：2007-02-01 00:00:00

abstract：:Biofilms in drinking water distribution systems (DWDSs) could cause several types of problems, such as the deterioration of water quality, corrosion of pipe walls, and potential proliferation of opportunistic pathogens. In this study, ten biofilm samples from different pipe materials, including ductile cast iron pipe ...

journal_title：Applied microbiology and biotechnology

authors： Ren H,Wang W,Liu Y,Liu S,Lou L,Cheng D,He X,Zhou X,Qiu S,Fu L,Liu J,Hu B

更新日期：2015-12-01 00:00:00

abstract：:Because engineering of the 101.016-bp megaplasmid pKB1 of Gordonia westfalica Kb1 failed due to the absence of an effective transfer system, pKB1 was transferred by conjugation from G. westfalica Kb1 to a kanamycin-resistant mutant of Rhodococcus opacus PD630 at a frequency of about 6.2 x 10(-8) events per recipient c...

journal_title：Applied microbiology and biotechnology

authors： Bröker D,Arenskötter M,Steinbüchel A

更新日期：2008-01-01 00:00:00

abstract：:Aspartic proteases exhibit optimum enzyme activity under acidic conditions and have been extensively used in food, fermentation, and leather industries. In this study, a novel aspartic protease precursor (proTlAPA1) from Talaromyces leycettanus was identified and successfully expressed in Pichia pastoris. Subsequently...

journal_title：Applied microbiology and biotechnology

authors： Guo Y,Tu T,Zheng J,Ren Y,Wang Y,Bai Y,Su X,Wang Y,Yao B,Huang H,Luo H

更新日期：2020-06-01 00:00:00

abstract：:Magnetotactic bacteria are difficult to grow under defined conditions in culture, which has presented a major obstacle to commercial application of magnetosomes. We studied the relationships among the cell growth, magnetosome formation, dissolved oxygen concentration (DO), and the ability to supply oxygen to the cells...

journal_title：Applied microbiology and biotechnology

authors： Sun JB,Zhao F,Tang T,Jiang W,Tian JS,Li Y,Li JL

更新日期：2008-06-01 00:00:00

abstract：:The novel influenza A virus (IAV) defective interfering particle "OP7" inhibits IAV replication in a co-infection and was previously suggested as a promising antiviral agent. Here, we report a batch-mode cell culture-based production process for OP7. In the present study, a seed virus containing standard virus (STV) a...

journal_title：Applied microbiology and biotechnology

authors： Hein MD,Kollmus H,Marichal-Gallardo P,Püttker S,Benndorf D,Genzel Y,Schughart K,Kupke SY,Reichl U

更新日期：2021-01-01 00:00:00

abstract：:The genome of the coprophilous fungus Podospora anserina displays an impressive array of genes encoding hemicellulolytic enzymes. In this study, we focused on a putative carbohydrate esterase (CE) from family 16 (CE16) that bears a carbohydrate-binding module from family CBM1. The protein was heterologously expressed ...

journal_title：Applied microbiology and biotechnology

authors： Puchart V,Berrin JG,Haon M,Biely P

更新日期：2015-12-01 00:00:00